Poolman, prof. dr. Bert
Bert Poolman is opgeleid in de bio-energetica en microbiologie, en schakelde later over naar de biochemie en biofysica. De centrale vragen in zijn onderzoek luiden: Hoe dringen moleculen door biologische membranen en hoe kunnen we stromen van opgeloste stoffen reguleren? Gedurende zijn hele loopbaan heeft Poolman grote bijdragen geleverd aan een beter begrip van de dynamiek en structuur van biologische membranen en aan het vakgebied van de vector-biochemie, dat wil zeggen de rol die elektrochemische gradiënten spelen in de aandrijving en regulering van membraantransport. Poolman is een vooraanstaande figuur op het gebied van de membraantransport en de wijze waarop cellen hun volume reguleren, alsmede de ontwikkeling van innovatieve technologieën op het gebied van membraanreconstructie. Hij heeft vooruitgang gebracht in het vakgebied van ABC (‘ATP-binding cassette’)-transporters, een van de grootste bekende proteïne-families, door functioneel en structureel onderzoek met elkaar te combineren.
Labyrint Napraatsessie over extremofielen en de wereld van microben
Poolman is directeur van het Groningen Biomolecular Sciences and Biotechnology Instituut en wetenschappelijk directeur van het Centrum voor Synthetische Biologie aan de Rijksuniversiteit Groningen. Hij heeft meer dan 270 publicaties op zijn naam staan in ‘peer refereed’ internationale wetenschappelijke tijdschriften. Hij begeleidde circa 50 promovendi. Bert Poolman is erkend als ‘ISI highly-cited’ onderzoeker en is bestuurslid van de ‘Faculty of 1000’. Sinds 2009 is hij lid van de KNAW.
In april 2015 ontving Poolman een Europese ERC Advanced Grant van 2,5 miljoen euro. Poolman zal daarmee een synthetische cel bouwen om de volumeregulering van een levende cel na te bootsen en te bestuderen.
Eerder in het nieuws
2024
Monterroso,
B., Margolin, W., Boersma, A. J., Rivas, G., Poolman,
B., & Zorrilla, S. (2024). Macromolecular
Crowding, Phase Separation, and Homeostasis in the Orchestration of
Bacterial Cellular Functions. Chemical
reviews, 124(4), 1899–1949. Article
3c00622. https://doi.org/10.1021/acs.chemrev.3c00622
van
der Sleen, L., Stevens, J. A., Marrink,
S. J., Poolman, B., & Tych, K.
(2024). Probing the stability and interdomain interactions in
the ABC transporter OpuA using single-molecule optical
tweezers. Cell reports,
43(4), Article 114110. Advance online publication. https://doi.org/10.1016/j.celrep.2024.114110
2023
Minh
Tran, B., Michiel Punter, C., Linnik,
D., Iyer, A., & Poolman, B.
(2024). Single-protein diffusion in the periplasm of
Escherichia coli. Journal of Molecular
Biology, 436(4), Article 168420. https://doi.org/10.1016/j.jmb.2023.168420
Bailoni,
E., Partipilo, M., Coenradij,
J., Grundel, D. A. J., Slotboom, D.
J., & Poolman, B. (2023). Minimal
Out-of-Equilibrium Metabolism for Synthetic Cells: A Membrane
Perspective. ACS Synthetic Biology,
12. https://doi.org/10.1021/acssynbio.3c00062
Poolman,
B. (2023). Physicochemical homeostasis in
bacteria. FEMS Microbiology Reviews,
47(4), Article fuad033. https://doi.org/10.1093/femsre/fuad033
Mantovanelli,
L., Linnik, D. S., Punter,
M., Kojakhmetov, H. J., Śmigiel, W.
M., & Poolman, B. (2023). Simulation-based
Reconstructed Diffusion unveils the effect of aging on protein
diffusion in Escherichia coli. PLoS Computational
Biology, 19(9), Article e1011093. https://doi.org/10.1371/journal.pcbi.1011093
Tran,
B. M., Linnik, D. S., Punter, C.
M., Śmigiel, W. M., Mantovanelli,
L., Iyer, A., O’Byrne, C., Abee, T.,
Johansson, J., & Poolman, B. (2023).
Super-resolving microscopy reveals the localizations and
movement dynamics of stressosome proteins in Listeria
monocytogenes. Communications biology,
6(1), Article 51. https://doi.org/10.1038/s42003-023-04423-y
2022
Bailoni,
E., & Poolman, B. (2022). ATP
Recycling Fuels Sustainable Glycerol 3-Phosphate Formation in
Synthetic Cells Fed by Dynamic Dialysis. ACS
Synthetic Biology, 11(7), 2348–2360.
Article 2c00075. https://doi.org/10.1021/acssynbio.2c00075
Frallicciardi,
J., Gabba, M., & Poolman, B. (2022).
Determining small-molecule permeation through lipid
membranes. Nature protocols,
17, 2620-2646. https://doi.org/10.1038/s41596-022-00734-2
Frallicciardi,
J., Melcr, J., Siginou, P.,
Marrink, S. J., & Poolman, B. (2022).
Membrane thickness, lipid phase and sterol type are
determining factors in the permeability of membranes to small
solutes. Nature Communications,
13(1), Article 1605. https://doi.org/10.1038/s41467-022-29272-x
Szomek,
M., Reinholdt, P., Walther, H. L., Scheidt, H. A., Müller,
P., Obermaier, S., Poolman, B., Kongsted,
J., & Wüstner, D. (2022). Natamycin sequesters
ergosterol and interferes with substrate transport by the lysine
transporter Lyp1 from yeast. Biochimica et
Biophysica Acta - Biomembranes, 1864(11),
Article 184012. https://doi.org/10.1016/j.bbamem.2022.184012
Smigiel,
W. M., Mantovanelli, L., Linnik, D.
S., Punter, M., Silberberg, J., Xiang, L., Xu,
K., & Poolman, B. (2022). Protein diffusion
in Escherichia coli cytoplasm scales with the mass ofthe complexes
and is location dependent. Science
Advances, 8(32), Article eabo5387. https://doi.org/10.1126/sciadv.abo5387
Aarts,
N., Bovenberg, R., Dogterom, M., van Est, R.,
Gerritsen, J., Macnaghten, P., Poolman, B., Robaey,
Z., Rasmussen, S., Ruivenkamp, G., Thole, E., Tremmel, G., van der
Vlugt, C., & Zwart, H. (2022). Society and synthetic
cells: A position paper by the Future Panel on Synthetic
Life. Rathenau Instituut.
2021
Iyer,
A., Baranov, M., Foster, A.
J., Chordia, S., Roelfes,
G., Vlijm, R., van den Bogaart,
G., & Poolman, B. (2021).
Chemogenetic Tags with Probe Exchange for Live-Cell
Fluorescence Microscopy. ACS chemical
biology, 16(5), 891-904. Article
acschembio.1c00100. https://doi.org/10.1021/acschembio.1c00100
Mantovanelli,
L., Gaastra, B. F., & Poolman,
B. (2021). Fluorescence-based sensing of the
bioenergetic and physicochemical status of the cell. In M.
A. Model, & I. Levitan (Eds.), Current Topics in Membranes:
New Methods and Sensors for Membrane and Cell Volume Research
(Vol. 88, pp. 1-54). (Current Topics in Membranes). ACADEMIC PRESS
INC ELSEVIER SCIENCE. https://doi.org/10.1016/bs.ctm.2021.10.002
Åberg,
C., & Poolman, B. (2021). Glass-Like
Characteristics of Intracellular Motion in Human Cells.
Biophysical Journal, 120(11),
2355-2366. https://doi.org/10.1016/j.bpj.2021.04.011
Alvarez-Sieiro,
P., Sikkema, H. R., & Poolman,
B. (2021). Heterodimer Formation of the Homodimeric
ABC Transporter OpuA. International Journal of
Molecular Sciences, 22(11), Article 5912. https://doi.org/10.3390/ijms22115912
Sikkema,
H. R., & Poolman, B. (2021). In
silico method for selecting residue pairs for single-molecule
microscopy and spectroscopy. Scientific
Reports, 11(1), Article 5756. https://doi.org/10.1038/s41598-021-85003-0
Tran,
B. M., Prabha, H., Iyer, A., O'Byrne, C., Abee,
T., & Poolman, B. (2021). Measurement of
Protein Mobility in Listeria monocytogenes Reveals a Unique
Tolerance to Osmotic Stress and Temperature Dependence of
Diffusion. Frontiers in Microbiology,
12, Article 640149. https://doi.org/10.3389/fmicb.2021.640149
Partipilo,
M., Ewins, E. J., Frallicciardi, J., Robinson,
T., Poolman, B., & Slotboom, D. J.
(2021). Minimal Pathway for the Regeneration of Redox
Cofactors. JACS Au, 1(12),
2280-2293. https://doi.org/10.1021/jacsau.1c00406
van
den Noort, M., de Boer, M., &
Poolman, B. (2021). Stability of Ligand-induced
Protein Conformation Influences Affinity in Maltose-binding
Protein. Journal of Molecular Biology,
433(15), Article 167036. https://doi.org/10.1016/j.jmb.2021.167036
Plötz,
E., Schuurman-Wolters, G., Zijlstra, N., Jager, A.,
Griffith, D., Guskov, A., Gouridis, G., Poolman,
B., & Cordes, T. (2021). Structural
and biophysical characterization of the tandem substrate-binding
domains of the ABC importer GlnPQ. Open
Biology, 11(4), Article 200406. https://doi.org/10.1098/rsob.200406
2020
Henderson,
R. K., de Valk, S. C., Poolman, B., & Mans, R.
(2021). Energy coupling of membrane transport and efficiency
of sucrose dissimilation in yeast. Metabolic
Engineering, 65, 243-254. https://doi.org/10.1016/j.ymben.2020.11.014
Pols,
T., Singh, S., Deelman-Driessen, C., Gaastra, B.
F., & Poolman, B. (2021). Enzymology
of the pathway for ATP production by arginine breakdown.
The FEBS Journal, 288(1), 293-309.
Article febs.15337. https://doi.org/10.1111/febs.15337
Liu,
B., Mavrova, S. N., van den Berg, J., Kristensen, S.
K., Mantovanelli, L., Veenhoff, L.
M., Poolman, B., & Boersma, A. J. (2020).
Erratum. ACS Sensors,
5(5), 1500-1500. https://doi.org/10.1021/acssensors.0c00744
Van't
Klooster, J. S., Bianchi, F., Doorn, R. B.,
Lorenzon, M., Lusseveld, J. H., Punter, C. M.,
& Poolman, B. (2020). Extracellular loops matter:
Subcellular location and function of the lysine transporter Lyp1
from Saccharomyces cerevisiae. The FEBS
Journal, 287(20), 4401-4414. Article
febs.15262. https://doi.org/10.1111/febs.15262
Sikkema,
H. R., van den Noort, M., Rheinberger,
J., de Boer, M., Krepel, S. T.,
Schuurman-Wolters, G. K., Paulino, C., &
Poolman, B. (2020). Gating by ionic strength and
safety check by cyclic-di-AMP in the ABC transporter OpuA.
Science Advances, 6(47), Article
eabd7697. https://doi.org/10.1126/sciadv.abd7697
Ruiz,
S. J., van 't Klooster, J. S., Bianchi,
F., & Poolman, B. (2020). Growth
Inhibition by Amino Acids in Saccharomyces cerevisiae.
Microorganisms, 9(1), 1-17. Article
9010007. https://doi.org/10.3390/microorganisms9010007
de
Boer, M., Cordes, T., & Poolman,
B. (2020). Kinetic Modelling of Transport Inhibition
by Substrates in ABC Importers. Journal of
Molecular Biology, 432(20), 5565-5576. https://doi.org/10.1016/j.jmb.2020.08.008
van
't Klooster, J. S., Cheng, T.-Y., Sikkema, H.
R., Jeucken, A., Moody, D. B., &
Poolman, B. (2020). Membrane Lipid Requirements of the
Lysine Transporter Lyp1 from Saccharomyces cerevisiae.
Journal of Molecular Biology,
432(14), 4023-4031. https://doi.org/10.1016/j.jmb.2020.04.029
van
't Klooster, J. S., Cheng, T.-Y., Sikkema, H.
R., Jeucken, A., Moody, B., &
Poolman, B. (2020). Periprotein lipidomes of
Saccharomyces cerevisiae provide a flexible environment for
conformational changes of membrane proteins.
eLife, 9, Article e57003. https://doi.org/10.7554/eLife.57003
Marinus,
N., Tahiri, N., Duca, M., Mouthaan, L. M. C.
M., Bianca, S., van den Noort,
M., Poolman, B., Witte, M.
D., & Minnaard, A. J. (2020).
Stereoselective Protection-Free Modification of
3-Keto-saccharides. Organic letters,
22(14), 5622-5626. https://doi.org/10.1021/acs.orglett.0c01986
Thomas,
C., Aller, S. G., Beis, K., Carpenter, E. P., Chang, G., Chen, L.,
Dassa, E., Dean, M., Duong Van Hoa, F., Ekiert, D., Ford, R.,
Gaudet, R., Gong, X., Holland, I. B., Huang, Y., Kahne, D. K.,
Kato, H., Koronakis, V., Koth, C. M., ... Tampé, R. (2020).
Structural and functional diversity calls for a new
classification of ABC transporters. FEBS
Letters, 594(23), 3767-3775. Article
1873-3468.13935. https://doi.org/10.1002/1873-3468.13935
2019
Syga,
L., De Vries, R. H., van Oosterhout, H., Bartelds, R.,
Boersma, A. J., Roelfes, G., & Poolman,
B. (2020). A trifunctional linker for palmitoylation
and peptide and protein localization in biological
membranes. ChemBioChem,
21(9), 1320-1328. https://doi.org/10.1002/cbic.201900655
Rempel,
I. L., Popken, P., Ghavami, A., Mishra, A., Hapsari,
R. A., Wolters, A. H. G., Veldsink, A.
C., Klaassens, M., Meinema, A. C., Poolman,
B., Giepmans, B. N. G., Onck, P.
R., Steen, A., & Veenhoff, L.
M. (2020). Flexible and Extended Linker Domains
Support Efficient Targeting of Heh2 to the Inner Nuclear
Membrane. Structure, 28(2),
185-195.e5. https://doi.org/10.1016/j.str.2019.11.003
Gabba,
M., & Poolman, B. (2020). Physiochemical
Modeling of Vesicle Dynamics upon Osmotic Upshift.
Biophysical Journal, 118(2),
435-447. Article bpj.2019.11.3383. https://doi.org/10.1016/j.bpj.2019.11.3383
Gabba,
M., Frallicciardi, J., van 't Klooster,
J., Henderson, R., Syga, Ł., Mans, R., van Maris, A. J.
A., & Poolman, B. (2020). Weak Acid
Permeation in Synthetic Lipid Vesicles and Across the Yeast Plasma
Membrane. Biophysical Journal,
118(2), 422-434. https://doi.org/10.1016/j.bpj.2019.11.3384
Price,
C. E., Branco Dos Santos, F., Hesseling, A., Uusitalo, J. J.,
Bachmann, H., Benavente, V., Goel, A., Berkhout, J., Bruggeman, F.
J., Marrink, S.-J., Montalban-Lopez, M., de
Jong, A., Kok, J., Molenaar, D., Poolman,
B., Teusink, B., & Kuipers, O. P. (2019).
Adaption to glucose limitation is modulated by the pleotropic
regulator CcpA, independent of selection pressure strength.
BMC Evolutionary Biology, 19(1),
Article 15. https://doi.org/10.1186/s12862-018-1331-x
Pols,
T., Sikkema, H. R., Gaastra, B. F.,
Frallicciardi, J., Śmigiel, W. M., Singh, S.,
& Poolman, B. (2019). A synthetic metabolic
network for physicochemical homeostasis. Nature
Communications, 10(1), Article 4239. https://doi.org/10.1038/s41467-019-12287-2
Sikkema,
H. R., Gaastra, B. F., Pols, T., &
Poolman, B. (2019). Cell fueling and metabolic energy
conservation in synthetic cells.
ChemBioChem, 20(20 SI),
2581–2592. https://doi.org/10.1002/cbic.201900398
de
Boer, M., Gkouridis, G., Vietrov, R., Begg, S. L.,
Schuurman-Wolters, G. K., Husada, F., Eleftheriadis, N.,
Poolman, B., McDevitt, C. A., & Cordes, T.
(2019). Conformational and dynamical plasticity in
substrate-binding proteins underlies selective transport in ABC
importers. eLife, 8, Article
e44652. https://doi.org/10.7554/eLife.44652
Liu,
B., Hasrat, Z., Poolman, B., & Boersma, A. J.
(2019). Decreased Effective Macromolecular Crowding in
Escherichia coli Adapted to Hyperosmotic Stress.
Journal of Bacteriology, 201(10),
Article e00708-18. https://doi.org/10.1128/JB.00708-18
Smigiel,
W. M., Lefrancois, P., & Poolman, B.
(2019). Physicochemical considerations for bottom-up
synthetic biology. Emerging topics in life
sciences, 3(5), 445-458. Article ETLS20190017.
https://doi.org/10.1042/ETLS20190017
Bianchi,
F., Van't Klooster, J. S., Ruiz, S. J., & Poolman,
B. (2019). Regulation of Amino Acid Transport in
Saccharomyces cerevisiae. Microbiology and
Molecular Biology Reviews, 83(4), Article
e00024-19. https://doi.org/10.1128/MMBR.00024-19
2018
Henderson,
R. K., Fendler, K., & Poolman, B. (2019).
Coupling efficiency of secondary active transporters.
Current Opinion in Biotechnology,
58, 62-71. https://doi.org/10.1016/j.copbio.2018.11.005
Marques,
W. L., Mans, R., Henderson, R. K., Marella, E. R., Horst, J. T.,
Hulster, E. D., Poolman, B., Daran, J.-M., Pronk, J.
T., Gombert, A. K., & van Maris, A. J. A. (2018).
Combined engineering of disaccharide transport and
phosphorolysis for enhanced ATP yield from sucrose fermentation in
Saccharomyces cerevisiae. Metabolic
Engineering, 45, 121-133. https://doi.org/10.1016/j.ymben.2017.11.012
Lycklama
A Nijeholt, J. A., Vietrov, R., Schuurman-Wolters, G.
K., & Poolman, B. (2018). Energy
coupling efficiency in the Type I ABC transporter GlnPQ.
Journal of Molecular Biology,
430(6), 853-866. https://doi.org/10.1016/j.jmb.2018.02.001
Schavemaker,
P. E., Boersma, A. J., & Poolman, B. (2018).
How Important Is Protein Diffusion in Prokaryotes?
Frontiers in Molecular Biosciences,
5, Article fmolb.2018.00093. https://doi.org/10.3389/fmolb.2018.00093
Schavemaker,
P. E., & Poolman, B. (2018). (Membrane)
Protein Production in Context. Trends in
Biochemical Sciences, 43(11), 858-868. https://doi.org/10.1016/j.tibs.2018.08.009
Syga,
Ł., Spakman, D., Punter, C. M., &
Poolman, B. (2018). Method for immobilization of
living and synthetic cells for high-resolution imaging and
single-particle tracking. Scientific
Reports, 8(1), Article 13789. https://doi.org/10.1038/s41598-018-32166-y
Bartelds,
R., Nematollahi, M. H., Pols, T., Stuart, M. C. A.,
Pardakhty, A., Asadikaram, G., & Poolman, B.
(2018). Niosomes, an alternative for liposomal
delivery. PLoS ONE, 13(4),
Article e0194179. https://doi.org/10.1371/journal.pone.0194179
Schuurman-Wolters,
G. K., de Boer, M., Pietrzyk, M. K.,
& Poolman, B. (2018). Protein linkers provide
limits on the domain interactions in the ABC importer GlnPQ and
determine the rate of transport. Journal of
Molecular Biology, 430(8), 1249-1262. Article
j.jmb.2018.02.014. https://doi.org/10.1016/j.jmb.2018.02.014
Bianchi,
F., Syga, Ł., Moiset, G., Spakman, D., Schavemaker, P.
E., Punter, C. M., Seinen, A.-B., van Oijen, A.
M., Robinson, A., & Poolman, B. (2018).
Steric exclusion and protein conformation determine the
localization of plasma membrane transporters.
Nature Communications, 9(1), Article
501. https://doi.org/10.1038/s41467-018-02864-2
Liu,
B., Mavrova, S. N., van den Berg, J., Kristensen, S.
K., Mantovanelli, L., Veenhoff, L.
M., Poolman, B., & Boersma, A. J. (2018).
The influence of fluorescent protein maturation on FRET
measurements in living cells. ACS
Sensors, 3(9), 1735-1742. https://doi.org/10.1021/acssensors.8b00473
2017
Cozzoli,
L., Gjonaj, L., Stuart, M. C. A., Poolman,
B., & Roelfes, G. (2018). Responsive
DNA G-quadruplex micelles. Chemical communications
(Cambridge, England), 54, 260-263. https://doi.org/10.1039/c7cc07899d
Franken,
L., Oostergetel, G., Pijning, T., Puri, P., Arkhipova,
V. I., Boekema, E., Poolman, B., & Guskov,
A. (2017). A general mechanism of ribosome
dimerization revealed by single-particle cryo-electron
microscopy. Nature Communications,
8, Article 722. https://doi.org/10.1038/s41467-017-00718-x
Liu,
B., Åberg, C., van Eerden, F. J., Marrink,
S., Poolman, B., & Boersma, A. J. (2017).
Design and Properties of Genetically Encoded Probes for
Sensing Macromolecular Crowding. Biophysical
Journal, 112(9), 1929-1939. https://doi.org/10.1016/j.bpj.2017.04.004
Liu,
B., Poolman, B., & Boersma, A. J. (2017).
Ionic strength sensing in living cells. ACS
chemical biology, 12(10), 2510-2514. Article
acschembio.7b00348. https://doi.org/10.1021/acschembio.7b00348
Bartelds,
R., Barnoud, J., J. Boersma, A., J. Marrink, S.,
& Poolman, B. (2017). Lipid phase separation in
the presence of hydrocarbons in giant unilamellar vesicles.
AIMS Biophysics, 4(4), 528-542. https://doi.org/10.3934/biophy.2017.4.528
van
den Berg, J., Boersma, A. J., & Poolman, B.
(2017). Microorganisms maintain crowding homeostasis.
Nature Reviews Microbiology, 15,
309-318. Article nrmicro.2017.17. https://doi.org/10.1038/nrmicro.2017.17
Henderson,
R., & Poolman, B. (2017). Proton-solute
coupling mechanism of the maltose transporter from Saccharomyces
cerevisiae. Scientific Reports,
7(1), 1-12. Article 14375. https://doi.org/10.1038/s41598-017-14438-1
Van
Meervelt, V., Soskine, M., Singh, S., Schuurman-Wolters,
G., Wijma, H. J., Poolman,
B., & Maglia, G. (2017). Real-time
conformational changes and controlled orientation of native
proteins inside a protein nanoreactor. Journal of
the American Chemical Society, 139(51),
18640-18646. Article jacs.7b10106. https://doi.org/10.1021/jacs.7b10106
Schavemaker,
P. E., Śmigiel, W. M., & Poolman, B. (2017).
Ribosome surface properties may impose limits on the nature
of the cytoplasmic proteome. eLife,
6, Article eLife.30084. https://doi.org/10.7554/eLife.30084
Poolman,
B., & Noireaux, V. (2017). Synthetic Biology: From
engineering living systems to the bottom=up construction of
synthetic cells. In J. van Doorn, J. Luirink, J. van der
Oost, B. Oudega, L. Robertson, H. Smit, P. Willemsen, & C.
Biemans (Eds.), Mighty Microbes: the amazing world of
microorganisms (1 ed., Vol. 1, pp. 215-217). Microcanon
Foundation. https://www.noireauxlab.org/
2016
Scheepers,
G. H., Lycklama A Nijeholt, J. A., & Poolman, B.
(2016). An updated structural classification of
substrate-binding proteins. FEBS
Letters, 590(23), 4393-4401. https://doi.org/10.1002/1873-3468.12445
Popov-Čeleketić,
D., Bianchi, F., Ruiz, S. J., Meutiawati, F.,
& Poolman, B. (2016). A Plasma Membrane
Association Module in Yeast Amino Acid Transporters.
The Journal of Biological Chemistry,
291(31), 16024-16037. https://doi.org/10.1074/jbc.M115.706770
Bianchi,
F., Klooster, J. S. V. ., Ruiz, S. J., Luck,
K., Pols, T., Urbatsch, I. L., & Poolman, B.
(2016). Asymmetry in inward- and outward-affinity constant of
transport explain unidirectional lysine flux in Saccharomyces
cerevisiae. Scientific Reports,
6, 1-13. Article 31443. https://doi.org/10.1038/srep31443
van
den Berg, J., & Poolman, B. (2016).
Compartmental Volume Regulation of E. Coli under Various
Growth Conditions. Biophysical
Journal, 110(3), 35A-35A. https://doi.org/10.1016/j.bpj.2015.11.259
Ruiz,
S. J., Schuurman-Wolters, G. K., & Poolman, B.
(2016). Crystal Structure of the Substrate-Binding Domain
from Listeria monocytogenes Bile-Resistance Determinant
BilE. Liquid Crystals, 6(12),
1-16. Article cryst6120162. https://doi.org/10.3390/cryst6120162
Maglia,
G., Soskine, M., Biesemans, A., Meervelt, V., &
Poolman, B. (2016). Nanopores with
internal protein adaptors. (Patent No.
WO2016166232). https://worldwide.espacenet.com/publicationDetails/originalDocument?CC=WO&NR=2016166232A1&KC=A1&FT=D&ND=3&date=20161020&DB=&locale=en_EP
van
den Berg, J., Galbiati, H., Rasmussen, A., Miller, S., &
Poolman, B. (2016). On the mobility, membrane location
and functionality of mechanosensitive channels in Escherichia
coli. Scientific Reports, 6,
Article 32709 . https://doi.org/10.1038/srep32709
van
Gijtenbeek, L. A., Robinson, A., van Oijen , A.,
Poolman, B., & Kok, J. (2016). On the Spatial
Organization of mRNA, Plasmids, and Ribosomes in a Bacterial Host
Overexpressing Membrane Proteins. PLoS
genetics, 12(12), 1-28. Article e1006523. https://doi.org/10.1371/journal.pgen.1006523
Boersma,
A., Liu, B., Åberg, C., & Poolman,
B. (2016). Quantification of Macromolecular Crowding
in Living Cells. Biophysical Journal,
110(3), 368A-368A. https://doi.org/10.1016/j.bpj.2015.11.1984
2015
Fulyani,
F., Schuurman-Wolters, G., Slotboom,
D.-J., & Poolman, B. (2016). Relative
rates of amino acid import via the ABC transporter GlnPQ determine
the growth performance of Lactococcus lactis.
Journal of Bacteriology, 198(3),
477-485. Article JB.00685-15. https://doi.org/10.1128/JB.00685-15
Swier,
L. J. Y. M., Slotboom, D., & Poolman,
B. (2015). ABC Importers. In A. M. George
(Ed.), ABC Transporters - 40 Years on (1 ed., Vol. 1, pp.
3-36). Springer Verlag. https://doi.org/10.1007/978-3-319-23476-2_1
Laba,
J. K., Steen, A., Popken, P., Chernova, A.,
Poolman, B., & Veenhoff, L. M. (2015).
Active Nuclear Import of Membrane Proteins Revisited.
Cells, 4(4), 653-673. https://doi.org/10.3390/cells4040653
Boersma,
A. J., Zuhorn, I. S., & Poolman, B.
(2015). A sensor for quantification of macromolecular
crowding in living cells. Nature
Methods, 12(3), 227-229. https://doi.org/10.1038/nmeth.3257
Gouridis,
G., Schuurman-Wolters, G., Ploetz, E., Husada, F.,
Vietrov, R., de Boer, M., Cordes,
T., & Poolman, B. (2015).
Conformational dynamics in substrate-binding domains
influences transport in the ABC importer GlnPQ.
Nature Structural & Molecular Biology,
22(1), 57-64. https://doi.org/10.1038/nsmb.2929
Spitzer,
J., Pielak, G. J., & Poolman, B. (2015).
Emergence of life: Physical chemistry changes the
paradigm. Biology Direct,
10(33). https://doi.org/10.1186/s13062-015-0060-y
Ho,
F. Y., & Poolman, B. (2015).
Engineering Escherichia coli for Functional Expression of
Membrane Proteins. Methods in
Enzymology, 556, 3-21. https://doi.org/10.1016/bs.mie.2015.01.003
Driessen,
A. J. M., & Poolman, B. (2015). In
memoriam: Wilhelmus Nicolaas Konings (1937-2014).
Extremophiles, 19, 233-234. https://doi.org/10.1007/s00792-015-0736-3
Kralt,
A., Carretta, M., Mari, M., Reggiori,
F., Steen, A., Poolman, B.,
& Veenhoff, L. M. (2015). Intrinsically disordered
linker and plasma membrane-binding motif sort ist2 and ssy1 to
junctions. Traffic, 16(2),
135-147. https://doi.org/10.1111/tra.12243
Goel,
A., Eckhardt, T. H., Puri, P., de Jong, A., Branco dos
Santos, F., Giera, M., Fusetti, F., de Vos, W. M., Kok,
J., Poolman, B., Molenaar, D., Kuipers,
O. P., & Teusink, B. (2015). Protein costs do not
explain evolution of metabolic strategies and regulation of
ribosomal content: does protein investment explain an anaerobic
bacterial Crabtree effect? Molecular
Microbiology, 97(1), 77-92. https://doi.org/10.1111/mmi.13012
Zollmann,
T., Moiset Coll, G., Tumulka, F., Tampé, R., Poolman,
B., & Abele, R. (2015). Single liposome analysis
of peptide translocation by the ABC transporter TAPL.
Proceedings of the National Academy of Sciences of the
United States of America, 112(7), 2046-2051.
https://doi.org/10.1073/pnas.1418100112
Popken,
P., Ghavami, A., Onck, P. R., Poolman,
B., & Veenhoff, L. M. (2015).
Size-dependent leak of soluble and membrane proteins through
the yeast nuclear pore complex. Molecular Biology
of the Cell, 26(7), 1386-1394. https://doi.org/10.1091/mbc.E14-07-1175
Husada,
F., Gouridis, G., Vietrov, R., Schuurman-Wolters, G.
K., Ploetz, E., de Boer, M., Poolman,
B., & Cordes, T. (2015). Watching
conformational dynamics of ABC transporters with single-molecule
tools. Biochemical Society
Transactions, 43(5), 1041-1047. https://doi.org/10.1042/BST20150140
2014
Velasquez
Guzman, J., Schuurman-Wolters, G., Birkner, J.
P., Abee, T., & Poolman, B. (2014).
Bacillus subtilis spore protein SpoVAC functions as a
mechanosensitive channel. Molecular
Microbiology. https://doi.org/10.1111/mmi.12591
Moiset,
G., López, C. A., Bartelds, R., Syga, L., Rijpkema,
E., Cukkemane, A., Baldus, M., Poolman,
B., & Marrink, S. J. (2014).
Disaccharides Impact the Lateral Organization of Lipid
Membranes. Journal of the American Chemical
Society, 136(46), 16167-16175. https://doi.org/10.1021/ja505476c
Gul,
N., Linares, D. M., Ho, F. Y., & Poolman,
B. (2014). Evolved Escherichia coli Strains for
Amplified, Functional Expression of Membrane Proteins.
Journal of Molecular Biology,
426(1), 136-149. https://doi.org/10.1016/j.jmb.2013.09.009
Mika,
J., Schavemaker, P., Krasnikov, V., &
Poolman, B. (2014). Impact of osmotic stress on
protein diffusion in Lactococcus lactis. Molecular
Microbiology, 94(4), 857-870. https://doi.org/10.1111/mmi.12800
Poolman,
B., Martinez Linares, D., & Gul, N. (2014). Improved means and methods for expressing
recombinant proteins. (Patent No. EP2796556). https://nl.espacenet.com/publicationDetails/originalDocument?CC=EP&NR=2796556A1&KC=A1&FT=D&ND=3&date=20141029&DB=&locale=nl_NL
Puri,
P., Eckhardt, T. H., Franken, L. E., Fusetti, F., Stuart, M.
C. A., Boekema, E. J., Kuipers, O. P.,
Kok, J., & Poolman, B. (2014).
Lactococcus lactis YfiA is necessary and sufficient for
ribosome dimerization. Molecular
Microbiology, 91(2), 394-407. https://doi.org/10.1111/mmi.12468
Driessen,
A., Kaback, H. R., Poolman, B., & Walker,
J. E. (2014). Obituary: Wilhelmus Nicolaas Konings
(1937-2014). Biochemist e-volution,
36(6), 42-43.
Puri,
P., Goel, A., Bochynska, A., & Poolman, B. (2014).
Regulation of acetate kinase isozymes and its importance for
mixed-acid fermentation in Lactococcus lactis.
Journal of Bacteriology, 196(7),
1386-1393. https://doi.org/10.1128/JB.01277-13
Franciosi,
L., Postma, D. S., van den Berge,
M., Govorukhina, N., Horvatovich, P.
L., Fusetti, F., Poolman, B., Lodewijk, M.
E., Timens, W., Bischoff, R., &
ten Hacken, N. (2014). Susceptibility to COPD:
Differential Proteomic Profiling after Acute Smoking.
PLoS ONE, 9(7), Article e102037. https://doi.org/10.1371/journal.pone.0102037
Puri,
P., Wetzel, C., Saffert, P., Gaston, K. W., Russel, S. P., Cordero
Varela, J. A., van der Vlies, P., Zhang, G., Limbach, P. A.,
Ignatova, Z., & Poolman, B. (2014).
Systematic identification of tRNAome and its dynamics in
Lactococcus lactis. Molecular
Microbiology, 93(5), 944-956. https://doi.org/10.1111/mmi.12710
Jansma,
M., Aalbers, F. S., & Poolman, B. (2014).
Van lezen naar schrijven. In W. Hoekstra, T. van
Maris, B. Poolman, J. Pronk, & R. Buiter (Eds.),
Synthetische Biologie: Creatief met Cellen (cahier 4 ed.,
Vol. 33, pp. 7-19). (Cahier Biowetenschappen en Maatschappij).
Drukkerij Tesink, Zutphen. http://tijdschrift.biomaatschappij.nl
2013
Moiset,
G., Cirac, A. D., Stuart, M. C. A., Marrink,
S.-J., Sengupta, D., & Poolman, B. (2013).
Dual action of BPC194: a membrane active peptide killing
bacterial cells. PLoS ONE,
8(4), Article e61541. https://doi.org/10.1371/journal.pone.0061541
Fulyani,
F., Schuurman-Wolters, G. K., Vujicic - Zagar,
A., Guskov, A., Slotboom, D.-J.,
& Poolman, B. (2013). Functional Diversity of
Tandem Substrate-Binding Domains in ABC Transporters from
Pathogenic Bacteria. Structure,
21(10), 1879-1888. https://doi.org/10.1016/j.str.2013.07.020
Gul,
N., & Poolman, B. (2013). Functional
reconstitution and osmoregulatory properties of the ProU ABC
transporter from Escherichia coli. Molecular
Membrane Biology, 30(2), 138-148. https://doi.org/10.3109/09687688.2012.754060
| Laatst gewijzigd: | 09 juni 2023 20:36 |
View this page in: English