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Poolman, Prof. Bert

Bert Poolman
Bert Poolman
Bert Poolman was trained in bioenergetics and microbiology and moved to biochemistry and biophysics in later years. Central questions in his research are: How do molecules permeate biological membranes and how can one control solute fluxes? Throughout his career, he has made seminal contributions to the understanding of the dynamics and permeability of biological membranes and to the field of vectorial biochemistry, i.e. the role of electrochemical gradients in the fuelling and regulation of membrane transport. Poolman has a leading record in the energetics of membrane transport and cellular osmoregulation as well as the development of innovative technologies in membrane reconstitution. He has advanced the field of ATP-binding cassette (ABC) transporters, one of the largest known protein families, by combining functional and structural studies.

Bert Poolman is scientific director of the Groningen Biomolecular Sciences and Biotechnology Institute, and he is program director of the Centre for Synthetic Biology. He has published over 270 articles in international peer-refereed scientific journals and supervised about 50 PhD students. He is ISI highly-cited researcher and member of the Faculty 1000 board. Poolman has been a member of the Royal Dutch Academy of Arts and Sciences (KNAW) since 2009.

In April 2015 Poolman was awarded a European ERC Advanced Grant of 2,5 million euros for his project 'ABCVolume: The ABC of Cell Volume Regulation'.

Previously in the news

Article for De Groene Amsterdammer
Article for De Groene Amsterdammer

2021

Iyer, A., Baranov, M., Foster, A. J., Chordia, S., Roelfes, G., Vlijm, R., van den Bogaart, G., & Poolman, B. (2021). Chemogenetic Tags with Probe Exchange for Live-Cell Fluorescence Microscopy. ACS chemical biology, 16(5), 891-904. [acschembio.1c00100]. https://doi.org/10.1021/acschembio.1c00100
Åberg, C., & Poolman, B. (2021). Glass-Like Characteristics of Intracellular Motion in Human Cells. Biophysical Journal, 120(11), 2355-2366. https://doi.org/10.1016/j.bpj.2021.04.011
Alvarez-Sieiro, P., Sikkema, H. R., & Poolman, B. (2021). Heterodimer Formation of the Homodimeric ABC Transporter OpuA. International Journal of Molecular Sciences, 22(11), [5912]. https://doi.org/10.3390/ijms22115912
Sikkema, H. R., & Poolman, B. (2021). In silico method for selecting residue pairs for single-molecule microscopy and spectroscopy. Scientific Reports, 11(1), [5756]. https://doi.org/10.1038/s41598-021-85003-0
Tran, B. M., Prabha, H., Iyer, A., O'Byrne, C., Abee, T., & Poolman, B. (2021). Measurement of Protein Mobility in Listeria monocytogenes Reveals a Unique Tolerance to Osmotic Stress and Temperature Dependence of Diffusion. Frontiers in Microbiology, 12, [640149]. https://doi.org/10.3389/fmicb.2021.640149
van den Noort, M., de Boer, M., & Poolman, B. (2021). Stability of Ligand-induced Protein Conformation Influences Affinity in Maltose-binding Protein. Journal of Molecular Biology, 433(15), [167036]. https://doi.org/10.1016/j.jmb.2021.167036
Plötz, E., Schuurman-Wolters, G., Zijlstra, N., Jager, A., Griffith, D., Guskov, A., Gouridis, G., Poolman, B., & Cordes, T. (2021). Structural and biophysical characterization of the tandem substrate-binding domains of the ABC importer GlnPQ. Open Biology, 11(4), [200406]. https://doi.org/10.1098/rsob.200406, https://doi.org/10.1098/rsob.200406

2020

Pols, T., Singh, S., Deelman-Driessen, C., Gaastra, B. F., & Poolman, B. (2021). Enzymology of the pathway for ATP production by arginine breakdown. The FEBS Journal, 288(1), 293-309. [febs.15337]. https://doi.org/10.1111/febs.15337
Henderson, R. K., de Valk, S. C., Poolman, B., & Mans, R. (2020). Energy coupling of membrane transport and efficiency of sucrose dissimilation in yeast. Metabolic Engineering. https://doi.org/10.1016/j.ymben.2020.11.014
Liu, B., Mavrova, S. N., van den Berg, J., Kristensen, S. K., Mantovanelli, L., Veenhoff, L. M., Poolman, B., & Boersma, A. J. (2020). Erratum. ACS Sensors, 5(5), 1500-1500. https://doi.org/10.1021/acssensors.0c00744
Van't Klooster, J. S., Bianchi, F., Doorn, R. B., Lorenzon, M., Lusseveld, J. H., Punter, C. M., & Poolman, B. (2020). Extracellular loops matter: Subcellular location and function of the lysine transporter Lyp1 from Saccharomyces cerevisiae. The FEBS Journal, 287(20), 4401-4414. [febs.15262]. https://doi.org/10.1111/febs.15262
Sikkema, H. R., van den Noort, M., Rheinberger, J., de Boer, M., Krepel, S. T., Schuurman-Wolters, G. K., Paulino, C., & Poolman, B. (2020). Gating by ionic strength and safety check by cyclic-di-AMP in the ABC transporter OpuA. Science Advances, 6(47), [eabd7697]. https://doi.org/10.1126/sciadv.abd7697
Ruiz, S. J., van 't Klooster, J. S., Bianchi, F., & Poolman, B. (2020). Growth Inhibition by Amino Acids in Saccharomyces cerevisiae. Microorganisms, 9(1), 1-17. [9010007]. https://doi.org/10.3390/microorganisms9010007
de Boer, M., Cordes, T., & Poolman, B. (2020). Kinetic Modelling of Transport Inhibition by Substrates in ABC Importers. Journal of Molecular Biology, 432(20), 5565-5576. https://doi.org/10.1016/j.jmb.2020.08.008
van 't Klooster, J. S., Cheng, T-Y., Sikkema, H. R., Jeucken, A., Moody, D. B., & Poolman, B. (2020). Membrane Lipid Requirements of the Lysine Transporter Lyp1 from Saccharomyces cerevisiae. Journal of Molecular Biology, 432(14), 4023-4031. https://doi.org/10.1016/j.jmb.2020.04.029
van 't Klooster, J. S., Cheng, T-Y., Sikkema, H. R., Jeucken, A., Moody, B., & Poolman, B. (2020). Periprotein lipidomes of Saccharomyces cerevisiae provide a flexible environment for conformational changes of membrane proteins. eLife, 9, [e57003]. https://doi.org/10.7554/eLife.57003
Marinus, N., Tahiri, N., Duca, M., Mouthaan, L. M. C. M., Bianca, S., van den Noort, M., Poolman, B., Witte, M. D., & Minnaard, A. J. (2020). Stereoselective Protection-Free Modification of 3-Keto-saccharides. Organic letters, 22(14), 5622-5626. https://doi.org/10.1021/acs.orglett.0c01986
Thomas, C., Aller, S. G., Beis, K., Carpenter, E. P., Chang, G., Chen, L., Dassa, E., Dean, M., Duong Van Hoa, F., Ekiert, D., Ford, R., Gaudet, R., Gong, X., Holland, I. B., Huang, Y., Kahne, D. K., Kato, H., Koronakis, V., Koth, C. M., ... Tampé, R. (2020). Structural and functional diversity calls for a new classification of ABC transporters. FEBS Letters, 594(23), 3767-3775. [1873-3468.13935]. https://doi.org/10.1002/1873-3468.13935

2019

Syga, L., De Vries, R. H., van Oosterhout, H., Bartelds, R., Boersma, A. J., Roelfes, G., & Poolman, B. (2020). A trifunctional linker for palmitoylation and peptide and protein localization in biological membranes. ChemBioChem, 21(9), 1320-1328. https://doi.org/10.1002/cbic.201900655
Rempel, I. L., Popken, P., Ghavami, A., Mishra, A., Hapsari, R. A., Wolters, A. H. G., Veldsink, A. C., Klaassens, M., Meinema, A. C., Poolman, B., Giepmans, B. N. G., Onck, P. R., Steen, A., & Veenhoff, L. M. (2020). Flexible and Extended Linker Domains Support Efficient Targeting of Heh2 to the Inner Nuclear Membrane. Structure, 28(2), 185-195.e5. https://doi.org/10.1016/j.str.2019.11.003
Gabba, M., & Poolman, B. (2020). Physiochemical Modeling of Vesicle Dynamics upon Osmotic Upshift. Biophysical Journal, 118(2), 435-447. [bpj.2019.11.3383]. https://doi.org/10.1016/j.bpj.2019.11.3383
Gabba, M., Frallicciardi, J., van 't Klooster, J., Henderson, R., Syga, Ł., Mans, R., van Maris, A. J. A., & Poolman, B. (2020). Weak Acid Permeation in Synthetic Lipid Vesicles and Across the Yeast Plasma Membrane. Biophysical Journal, 118(2), 422-434. https://doi.org/10.1016/j.bpj.2019.11.3384
Pols, T., Sikkema, H. R., Gaastra, B. F., Frallicciardi, J., Śmigiel, W. M., Singh, S., & Poolman, B. (2019). A synthetic metabolic network for physicochemical homeostasis. Nature Communications, 10(1), [4239]. https://doi.org/10.1038/s41467-019-12287-2
Price, C. E., Branco Dos Santos, F., Hesseling, A., Uusitalo, J. J., Bachmann, H., Benavente, V., Goel, A., Berkhout, J., Bruggeman, F. J., Marrink, S-J., Montalban-Lopez, M., de Jong, A., Kok, J., Molenaar, D., Poolman, B., Teusink, B., & Kuipers, O. P. (2019). Adaption to glucose limitation is modulated by the pleotropic regulator CcpA, independent of selection pressure strength. BMC Evolutionary Biology, 19(1), [15]. https://doi.org/10.1186/s12862-018-1331-x
Sikkema, H. R., Gaastra, B. F., Pols, T., & Poolman, B. (2019). Cell fueling and metabolic energy conservation in synthetic cells. ChemBioChem, 20(20 SI), 2581 –2592. https://doi.org/10.1002/cbic.201900398
de Boer, M., Gkouridis, G., Vietrov, R., Begg, S. L., Schuurman-Wolters, G. K., Husada, F., Eleftheriadis, N., Poolman, B., McDevitt, C. A., & Cordes, T. (2019). Conformational and dynamical plasticity in substrate-binding proteins underlies selective transport in ABC importers. eLife, 8, [e44652]. https://doi.org/10.7554/eLife.44652
Liu, B., Hasrat, Z., Poolman, B., & Boersma, A. J. (2019). Decreased Effective Macromolecular Crowding in Escherichia coli Adapted to Hyperosmotic Stress. Journal of Bacteriology, 201(10), [e00708-18]. https://doi.org/10.1128/JB.00708-18
Smigiel, W. M., Lefrancois, P., & Poolman, B. (2019). Physicochemical considerations for bottom-up synthetic biology. Emerging topics in life sciences, 3(5), 445-458. [ETLS20190017]. https://doi.org/10.1042/ETLS20190017
Bianchi, F., Van't Klooster, J. S., Ruiz, S. J., & Poolman, B. (2019). Regulation of Amino Acid Transport in Saccharomyces cerevisiae. Microbiology and Molecular Biology Reviews, 83(4), [e00024-19]. https://doi.org/10.1128/MMBR.00024-19

2018

Henderson, R. K., Fendler, K., & Poolman, B. (2019). Coupling efficiency of secondary active transporters. Current Opinion in Biotechnology, 58, 62-71. https://doi.org/10.1016/j.copbio.2018.11.005
Marques, W. L., Mans, R., Henderson, R. K., Marella, E. R., Horst, J. T., Hulster, E. D., Poolman, B., Daran, J-M., Pronk, J. T., Gombert, A. K., & van Maris, A. J. A. (2018). Combined engineering of disaccharide transport and phosphorolysis for enhanced ATP yield from sucrose fermentation in Saccharomyces cerevisiae. Metabolic Engineering, 45, 121-133. https://doi.org/10.1016/j.ymben.2017.11.012
Lycklama A Nijeholt, J. A., Vietrov, R., Schuurman-Wolters, G. K., & Poolman, B. (2018). Energy coupling efficiency in the Type I ABC transporter GlnPQ. Journal of Molecular Biology, 430(6), 853-866. https://doi.org/10.1016/j.jmb.2018.02.001
Schavemaker, P. E., Boersma, A. J., & Poolman, B. (2018). How Important Is Protein Diffusion in Prokaryotes? Frontiers in Molecular Biosciences, 5, [fmolb.2018.00093]. https://doi.org/10.3389/fmolb.2018.00093
Schavemaker, P. E., & Poolman, B. (2018). (Membrane) Protein Production in Context. Trends in Biochemical Sciences, 43(11), 858-868. https://doi.org/10.1016/j.tibs.2018.08.009
Syga, Ł., Spakman, D., Punter, C. M., & Poolman, B. (2018). Method for immobilization of living and synthetic cells for high-resolution imaging and single-particle tracking. Scientific Reports, 8(1), [13789]. https://doi.org/10.1038/s41598-018-32166-y
Bartelds, R., Nematollahi, M. H., Pols, T., Stuart, M. C. A., Pardakhty, A., Asadikaram, G., & Poolman, B. (2018). Niosomes, an alternative for liposomal delivery. PLoS ONE, 13(4), [e0194179]. https://doi.org/10.1371/journal.pone.0194179
Schuurman-Wolters, G. K., de Boer, M., Pietrzyk, M. K., & Poolman, B. (2018). Protein linkers provide limits on the domain interactions in the ABC importer GlnPQ and determine the rate of transport. Journal of Molecular Biology, 430(8), 1249-1262. [j.jmb.2018.02.014]. https://doi.org/10.1016/j.jmb.2018.02.014
Bianchi, F., Syga, Ł., Moiset, G., Spakman, D., Schavemaker, P. E., Punter, C. M., Seinen, A-B., van Oijen, A. M., Robinson, A., & Poolman, B. (2018). Steric exclusion and protein conformation determine the localization of plasma membrane transporters. Nature Communications, 9(1), [501]. https://doi.org/10.1038/s41467-018-02864-2
Liu, B., Mavrova, S. N., van den Berg, J., Kristensen, S. K., Mantovanelli, L., Veenhoff, L. M., Poolman, B., & Boersma, A. J. (2018). The influence of fluorescent protein maturation on FRET measurements in living cells. ACS Sensors, 3(9), 1735-1742. https://doi.org/10.1021/acssensors.8b00473

2017

Cozzoli, L., Gjonaj, L., Stuart, M. C. A., Poolman, B., & Roelfes, G. (2018). Responsive DNA G-quadruplex micelles. Chemical communications (Cambridge, England), 54, 260-263. https://doi.org/10.1039/c7cc07899d
Franken, L., Oostergetel, G., Pijning, T., Puri, P., Arkhipova, V. I., Boekema, E., Poolman, B., & Guskov, A. (2017). A general mechanism of ribosome dimerization revealed by single-particle cryo-electron microscopy. Nature Communications, 8, [722]. https://doi.org/10.1038/s41467-017-00718-x
Liu, B., Åberg, C., van Eerden, F. J., Marrink, S., Poolman, B., & Boersma, A. J. (2017). Design and Properties of Genetically Encoded Probes for Sensing Macromolecular Crowding. Biophysical Journal, 112(9), 1929-1939. https://doi.org/10.1016/j.bpj.2017.04.004
Liu, B., Poolman, B., & Boersma, A. J. (2017). Ionic strength sensing in living cells. ACS chemical biology, 12(10), 2510-2514. [acschembio.7b00348]. https://doi.org/10.1021/acschembio.7b00348
Bartelds, R., Barnoud, J., J. Boersma, A., J. Marrink, S., & Poolman, B. (2017). Lipid phase separation in the presence of hydrocarbons in giant unilamellar vesicles. AIMS Biophysics, 4(4), 528-542. https://doi.org/10.3934/biophy.2017.4.528
van den Berg, J., Boersma, A. J., & Poolman, B. (2017). Microorganisms maintain crowding homeostasis. Nature Reviews Microbiology, 15, 309-318. [nrmicro.2017.17]. https://doi.org/10.1038/nrmicro.2017.17
Henderson, R., & Poolman, B. (2017). Proton-solute coupling mechanism of the maltose transporter from Saccharomyces cerevisiae. Scientific Reports, 7(1), 1-12. [14375]. https://doi.org/10.1038/s41598-017-14438-1
Van Meervelt, V., Soskine, M., Singh, S., Schuurman-Wolters, G., Wijma, H. J., Poolman, B., & Maglia, G. (2017). Real-time conformational changes and controlled orientation of native proteins inside a protein nanoreactor. Journal of the American Chemical Society, 139(51), 18640-18646. [jacs.7b10106]. https://doi.org/10.1021/jacs.7b10106
Schavemaker, P. E., Śmigiel, W. M., & Poolman, B. (2017). Ribosome surface properties may impose limits on the nature of the cytoplasmic proteome. eLife, 6, [eLife.30084]. https://doi.org/10.7554/eLife.30084
Poolman, B., & Noireaux, V. (2017). Synthetic Biology: From engineering living systems to the bottom=up construction of synthetic cells. In J. van Doorn, J. Luirink, J. van der Oost, B. Oudega, L. Robertson, H. Smit, P. Willemsen, & C. Biemans (Eds.), MIGHTY MICROBES: THE AMAZING WORLD OF MICROORGANISMS (1 ed., Vol. 1, pp. 215-217). Microcanon Foundation.

2016

Popov-Čeleketić, D., Bianchi, F., Ruiz, S. J., Meutiawati, F., & Poolman, B. (2016). A Plasma Membrane Association Module in Yeast Amino Acid Transporters. The Journal of Biological Chemistry, 291(31), 16024-16037. https://doi.org/10.1074/jbc.M115.706770
Scheepers, G. H., Lycklama A Nijeholt, J. A., & Poolman, B. (2016). An updated structural classification of substrate-binding proteins. FEBS Letters, 590(23), 4393-4401. https://doi.org/10.1002/1873-3468.12445
Bianchi, F., Klooster, J. S. V. ., Ruiz, S. J., Luck, K., Pols, T., Urbatsch, I. L., & Poolman, B. (2016). Asymmetry in inward- and outward-affinity constant of transport explain unidirectional lysine flux in Saccharomyces cerevisiae. Scientific Reports, 6, 1-13. [31443]. https://doi.org/10.1038/srep31443
van den Berg, J., & Poolman, B. (2016). Compartmental Volume Regulation of E. Coli under Various Growth Conditions. Biophysical Journal, 110(3), 35A-35A. https://doi.org/10.1016/j.bpj.2015.11.259
Ruiz, S. J., Schuurman-Wolters, G. K., & Poolman, B. (2016). Crystal Structure of the Substrate-Binding Domain from Listeria monocytogenes Bile-Resistance Determinant BilE. Liquid Crystals, 6(12), 1-16. [cryst6120162]. https://doi.org/10.3390/cryst6120162
Maglia, G., Soskine, M., Biesemans, A., Meervelt, V., & Poolman, B. (2016). Nanopores with internal protein adaptors. (Patent No. WO2016166232). https://worldwide.espacenet.com/publicationDetails/originalDocument?CC=WO&NR=2016166232A1&KC=A1&FT=D&ND=3&date=20161020&DB=&locale=en_EP
van den Berg, J., Galbiati, H., Rasmussen, A., Miller, S., & Poolman, B. (2016). On the mobility, membrane location and functionality of mechanosensitive channels in Escherichia coli. Scientific Reports, 6, [32709 ]. https://doi.org/10.1038/srep32709
van Gijtenbeek, L. A., Robinson, A., van Oijen , A., Poolman, B., & Kok, J. (2016). On the Spatial Organization of mRNA, Plasmids, and Ribosomes in a Bacterial Host Overexpressing Membrane Proteins. PLoS genetics, 12(12), 1-28. [e1006523]. https://doi.org/10.1371/journal.pgen.1006523
Boersma, A., Liu, B., Åberg, C., & Poolman, B. (2016). Quantification of Macromolecular Crowding in Living Cells. Biophysical Journal, 110(3), 368A-368A. https://doi.org/10.1016/j.bpj.2015.11.1984

2015

Fulyani, F., Schuurman-Wolters, G., Slotboom, D-J., & Poolman, B. (2016). Relative rates of amino acid import via the ABC transporter GlnPQ determine the growth performance of Lactococcus lactis. Journal of Bacteriology, 198(3), 477-485. [JB.00685-15]. https://doi.org/10.1128/JB.00685-15
Boersma, A. J., Zuhorn, I. S., & Poolman, B. (2015). A sensor for quantification of macromolecular crowding in living cells. Nature Methods, 12(3), 227-229. https://doi.org/10.1038/nmeth.3257
Swier, L. J. Y. M., Slotboom, D. J., & Poolman, B. (2015). ABC importers. In A. George (Ed.), ABC Transporters - 40 Years on (pp. 3-36). Springer International Publishing. https://doi.org/10.1007/978-3-319-23476-2_1
Swier, L. J. Y. M., Slotboom, D., & Poolman, B. (2015). ABC transporters: A structure-function perspective. In A. M. George (Ed.), ABC Transporters - 40 Years on (1 ed., Vol. 1, pp. 3-36). Springer Verlag.
Laba, J. K., Steen, A., Popken, P., Chernova, A., Poolman, B., & Veenhoff, L. M. (2015). Active Nuclear Import of Membrane Proteins Revisited. Cells, 4(4), 653-673. https://doi.org/10.3390/cells4040653
Gouridis, G., Schuurman-Wolters, G., Ploetz, E., Husada, F., Vietrov, R., de Boer, M., Cordes, T., & Poolman, B. (2015). Conformational dynamics in substrate-binding domains influences transport in the ABC importer GlnPQ. Nature Structural & Molecular Biology, 22(1), 57-64. https://doi.org/10.1038/nsmb.2929
Spitzer, J., Pielak, G. J., & Poolman, B. (2015). Emergence of life: Physical chemistry changes the paradigm. Biology Direct, 10(33). https://doi.org/10.1186/s13062-015-0060-y
Ho, F. Y., & Poolman, B. (2015). Engineering Escherichia coli for Functional Expression of Membrane Proteins. Methods in Enzymology, 556, 3-21. https://doi.org/10.1016/bs.mie.2015.01.003
Driessen, A. J. M., & Poolman, B. (2015). In memoriam: Wilhelmus Nicolaas Konings (1937-2014). Extremophiles, 19, 233-234. https://doi.org/10.1007/s00792-015-0736-3
Kralt, A., Carretta, M., Mari, M., Reggiori, F., Steen, A., Poolman, B., & Veenhoff, L. M. (2015). Intrinsically disordered linker and plasma membrane-binding motif sort ist2 and ssy1 to junctions. Traffic, 16(2), 135-147. https://doi.org/10.1111/tra.12243
Goel, A., Eckhardt, T. H., Puri, P., de Jong, A., Branco dos Santos, F., Giera, M., Fusetti, F., de Vos, W. M., Kok, J., Poolman, B., Molenaar, D., Kuipers, O. P., & Teusink, B. (2015). Protein costs do not explain evolution of metabolic strategies and regulation of ribosomal content: does protein investment explain an anaerobic bacterial Crabtree effect? Molecular Microbiology, 97(1), 77-92. https://doi.org/10.1111/mmi.13012
Zollmann, T., Moiset Coll, G., Tumulka, F., Tampé, R., Poolman, B., & Abele, R. (2015). Single liposome analysis of peptide translocation by the ABC transporter TAPL. Proceedings of the National Academy of Sciences of the United States of America, 112(7), 2046-2051. https://doi.org/10.1073/pnas.1418100112
Popken, P., Ghavami, A., Onck, P. R., Poolman, B., & Veenhoff, L. M. (2015). Size-dependent leak of soluble and membrane proteins through the yeast nuclear pore complex. Molecular Biology of the Cell, 26(7), 1386-1394. https://doi.org/10.1091/mbc.E14-07-1175
Husada, F., Gouridis, G., Vietrov, R., Schuurman-Wolters, G. K., Ploetz, E., de Boer, M., Poolman, B., & Cordes, T. (2015). Watching conformational dynamics of ABC transporters with single-molecule tools. Biochemical Society Transactions, 43(5), 1041-1047. https://doi.org/10.1042/BST20150140

2014

Velasquez Guzman, J., Schuurman-Wolters, G., Birkner, J. P., Abee, T., & Poolman, B. (2014). Bacillus subtilis spore protein SpoVAC functions as a mechanosensitive channel. Molecular Microbiology. https://doi.org/10.1111/mmi.12591
Moiset, G., López, C. A., Bartelds, R., Syga, L., Rijpkema, E., Cukkemane, A., Baldus, M., Poolman, B., & Marrink, S. J. (2014). Disaccharides Impact the Lateral Organization of Lipid Membranes. Journal of the American Chemical Society, 136(46), 16167-16175. https://doi.org/10.1021/ja505476c
Gul, N., Linares, D. M., Ho, F. Y., & Poolman, B. (2014). Evolved Escherichia coli Strains for Amplified, Functional Expression of Membrane Proteins. Journal of Molecular Biology, 426(1), 136-149. https://doi.org/10.1016/j.jmb.2013.09.009
Mika, J., Schavemaker, P., Krasnikov, V., & Poolman, B. (2014). Impact of osmotic stress on protein diffusion in Lactococcus lactis. Molecular Microbiology, 94(4), 857-870. https://doi.org/10.1111/mmi.12800
Poolman, B., Martinez Linares, D., & Gul, N. (2014). Improved means and methods for expressing recombinant proteins. (Patent No. EP2796556). https://nl.espacenet.com/publicationDetails/originalDocument?CC=EP&NR=2796556A1&KC=A1&FT=D&ND=3&date=20141029&DB=&locale=nl_NL
Puri, P., Eckhardt, T. H., Franken, L. E., Fusetti, F., Stuart, M. C. A., Boekema, E. J., Kuipers, O. P., Kok, J., & Poolman, B. (2014). Lactococcus lactis YfiA is necessary and sufficient for ribosome dimerization. Molecular Microbiology, 91(2), 394-407. https://doi.org/10.1111/mmi.12468
Driessen, A., Kaback, H. R., Poolman, B., & Walker, J. E. (2014). Obituary: Wilhelmus Nicolaas Konings (1937-2014). Biochemist e-volution, 36(6), 42-43.
Puri, P., Goel, A., Bochynska, A., & Poolman, B. (2014). Regulation of acetate kinase isozymes and its importance for mixed-acid fermentation in Lactococcus lactis. Journal of Bacteriology, 196(7), 1386-1393. https://doi.org/10.1128/JB.01277-13
Franciosi, L., Postma, D. S., van den Berge, M., Govorukhina, N., Horvatovich, P. L., Fusetti, F., Poolman, B., Lodewijk, M. E., Timens, W., Bischoff, R., & ten Hacken, N. (2014). Susceptibility to COPD: Differential Proteomic Profiling after Acute Smoking. PLoS ONE, 9(7), [e102037]. https://doi.org/10.1371/journal.pone.0102037
Puri, P., Wetzel, C., Saffert, P., Gaston, K. W., Russel, S. P., Cordero Varela, J. A., van der Vlies, P., Zhang, G., Limbach, P. A., Ignatova, Z., & Poolman, B. (2014). Systematic identification of tRNAome and its dynamics in Lactococcus lactis. Molecular Microbiology, 93(5), 944-956. https://doi.org/10.1111/mmi.12710
Jansma, M., Aalbers, F. S., & Poolman, B. (2014). Van lezen naar schrijven. In W. Hoekstra, T. van Maris, B. Poolman, J. Pronk, & R. Buiter (Eds.), Synthetische Biologie: Creatief met Cellen (cahier 4 ed., Vol. 33, pp. 7-19). (Cahier Biowetenschappen en Maatschappij). Drukkerij Tesink, Zutphen. http://tijdschrift.biomaatschappij.nl

2013

Moiset, G., Cirac, A. D., Stuart, M. C. A., Marrink, S-J., Sengupta, D., & Poolman, B. (2013). Dual action of BPC194: a membrane active peptide killing bacterial cells. PLoS ONE, 8(4), [e61541]. https://doi.org/10.1371/journal.pone.0061541
Fulyani, F., Schuurman-Wolters, G. K., Vujicic - Zagar, A., Guskov, A., Slotboom, D-J., & Poolman, B. (2013). Functional Diversity of Tandem Substrate-Binding Domains in ABC Transporters from Pathogenic Bacteria. Structure, 21(10), 1879-1888. https://doi.org/10.1016/j.str.2013.07.020
Gul, N., & Poolman, B. (2013). Functional reconstitution and osmoregulatory properties of the ProU ABC transporter from Escherichia coli. Molecular Membrane Biology, 30(2), 138-148. https://doi.org/10.3109/09687688.2012.754060
Spitzer, J., & Poolman, B. (2013). How crowded is the prokaryotic cytoplasm? FEBS Letters, 587(14), 2094-2098. https://doi.org/10.1016/j.febslet.2013.05.051
Remus, D. M., Bongers, R. S., Meijerink, M., Fusetti, F., Poolman, B., de Vos, P., Wells, J. M., Kleerebezem, M., & Bron, P. A. (2013). Impact of Lactobacillus plantarum Sortase on Target Protein Sorting, Gastrointestinal Persistence, and Host Immune Response Modulation. Journal of Bacteriology, 195(3), 502-509. https://doi.org/10.1128/JB.01321-12
Poolman, B., Linares, D. M., & Gul, N. (2013). Increased production of membrane proteins. (Patent No. P100103EP00).
van den Bogaart, G., Meinema, A. C., Krasnikov, V., Veenhoff, L. M., & Poolman, B. (2013). Nuclear transport factor directs localization of protein synthesis during mitosis (Corrigendum; vol 11, pg 350, 2009). Nature Cell Biology, 15(4), 441. https://doi.org/10.1038/ncb2728
Mika, J. T., Birkner, J. P., Poolman, B., & Kocer, A. (2013). On the role of individual subunits in MscL gating: "All for one, one for all?". The FASEB Journal, 27(3), 882-892. https://doi.org/10.1096/fj.12-214361
Karasawa, A., Swier, L. J. Y. M., Stuart, M. C. A., Brouwers, J., Helms, B., & Poolman, B. (2013). Physicochemical factors controlling the activity and energy coupling of an ionic strength-gated ATP-binding cassette (ABC) transporter. J. Biol. Chem., 288(41), 29862-29871. https://doi.org/10.1074/jbc.M113.499327
Franciosi, L., Govorukhina, N., Fusetti, F., Poolman, B., Lodewijk, M. E., Timens, W., Postma, D., ten Hacken, N., & Bischoff, R. (2013). Proteomic analysis of human epithelial lining fluid by microfluidics-based nanoLC-MS/MS: A feasibility study. Electrophoresis, 34(18), 2683-2694. https://doi.org/10.1002/elps.201300020
Meinema, A. C., Poolman, B., & Veenhoff, L. M. (2013). Quantitative Analysis of Membrane Protein Transport Across the Nuclear Pore Complex. Traffic, 14(5), 487-501. https://doi.org/10.1111/tra.12048
Poolman, B., & Slotboom, D-J. (2013). Substrate Capture by ABC Transporters. In G. C. K. Roberts (Ed.), Encyclopedia of Biophysics (pp. 2503-2509). Springer.

2012

Gandhi, T., Puri, P., Fusetti, F., Breitling, R., Poolman, B., & Permentier, H. P. (2012). Effect of iTRAQ Labeling on the Relative Abundance of Peptide Fragment Ions Produced by MALDI-MS/MS. Journal of Proteome Research, 11(8), 4044-4051. https://doi.org/10.1021/pr300083x
Gul, N., Schuurman-Wolters, G., Karasawa, A., & Poolman, B. (2012). Functional Characterization of Amphipathic alpha-Helix in the Osmoregulatory ABC Transporter OpuA. Biochemistry, 51(25), 5142-5152. https://doi.org/10.1021/bi300451a
Birkner, J. P., Poolman, B., & Kocer, A. (2012). Hydrophobic gating of mechanosensitive channel of large conductance evidenced by single-subunit resolution. Proceedings of the National Academy of Sciences of the United States of America, 109(32), 12944-12949. https://doi.org/10.1073/pnas.1205270109
Poolman, B. (2012). On the Role of CBS Domains in Osmoregulatory ABC Transporters. Biophysical Journal, 102(3), 626a-626a. https://doi.org/10.1016/j.bpj.2011.11.3410
Berntsson, R. P. -A., ter Beek, J., Dosz-Majsnerowska, M., Duurkens, R. H., Puri, P., Poolman, B., & Slotboom, D-J. (2012). Structural divergence of paralogous S components from ECF-type ABC transporters. Proceedings of the National Academy of Sciences of the United States of America, 109(35), 13990-13995. https://doi.org/10.1073/pnas.1203219109
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