Poolman, Prof. Bert
Bert Poolman was trained in bioenergetics and microbiology and moved to biochemistry and biophysics in later years. Central questions in his research are: How do molecules permeate biological membranes and how can one control solute fluxes? Throughout his career, he has made seminal contributions to the understanding of the dynamics and permeability of biological membranes and to the field of vectorial biochemistry, i.e. the role of electrochemical gradients in the fuelling and regulation of membrane transport. Poolman has a leading record in the energetics of membrane transport and cellular osmoregulation as well as the development of innovative technologies in membrane reconstitution. He has advanced the field of ATP-binding cassette (ABC) transporters, one of the largest known protein families, by combining functional and structural studies.
Bert Poolman is scientific director of the Groningen Biomolecular Sciences and Biotechnology Institute, and he is program director of the Centre for Synthetic Biology. He has published over 270 articles in international peer-refereed scientific journals and supervised about 50 PhD students. He is ISI highly-cited researcher and member of the Faculty 1000 board. Poolman has been a member of the Royal Dutch Academy of Arts and Sciences (KNAW) since 2009.
In April 2015 Poolman was awarded a European ERC Advanced Grant of 2,5 million euros for his project 'ABCVolume: The ABC of Cell Volume Regulation'.
Previously in the news
2023
Bailoni,
E., Partipilo, M., Coenradij,
J., Grundel, D. A. J., Slotboom, D.
J., & Poolman, B. (2023). Minimal
Out-of-Equilibrium Metabolism for Synthetic Cells: A Membrane
Perspective. ACS Synthetic Biology,
12. https://doi.org/10.1021/acssynbio.3c00062
Tran,
B. M., Linnik, D. S., Punter, C.
M., Śmigiel, W. M., Mantovanelli,
L., Iyer, A., O’Byrne, C., Abee, T.,
Johansson, J., & Poolman, B. (2023).
Super-resolving microscopy reveals the localizations and
movement dynamics of stressosome proteins in Listeria
monocytogenes. Communications biology,
6(1), [51]. https://doi.org/10.1038/s42003-023-04423-y
2022
Bailoni,
E., & Poolman, B. (2022). ATP
Recycling Fuels Sustainable Glycerol 3-Phosphate Formation in
Synthetic Cells Fed by Dynamic Dialysis. ACS
Synthetic Biology, 11(7), 2348–2360.
[2c00075]. https://doi.org/10.1021/acssynbio.2c00075
Frallicciardi,
J., Gabba, M., & Poolman, B. (2022).
Determining small-molecule permeation through lipid
membranes. Nature protocols,
17, 2620-2646. https://doi.org/10.1038/s41596-022-00734-2
Frallicciardi,
J., Melcr, J., Siginou, P.,
Marrink, S. J., & Poolman, B. (2022).
Membrane thickness, lipid phase and sterol type are
determining factors in the permeability of membranes to small
solutes. Nature Communications,
13(1), [1605]. https://doi.org/10.1038/s41467-022-29272-x
Szomek,
M., Reinholdt, P., Walther, H. L., Scheidt, H. A., Müller,
P., Obermaier, S., Poolman, B., Kongsted,
J., & Wüstner, D. (2022). Natamycin sequesters
ergosterol and interferes with substrate transport by the lysine
transporter Lyp1 from yeast. Biochimica et
Biophysica Acta - Biomembranes, 1864(11),
[184012]. https://doi.org/10.1016/j.bbamem.2022.184012
Smigiel,
W. M., Mantovanelli, L., Linnik, D.
S., Punter, M., Silberberg, J., Xiang, L., Xu,
K., & Poolman, B. (2022). Protein diffusion
in Escherichia coli cytoplasm scales with the mass ofthe complexes
and is location dependent. Science
Advances, 8(32), [eabo5387]. https://doi.org/10.1126/sciadv.abo5387
Aarts,
N., Bovenberg, R., Dogterom, M., van Est, R.,
Gerritsen, J., Macnaghten, P., Poolman, B., Robaey,
Z., Rasmussen, S., Ruivenkamp, G., Thole, E., Tremmel, G., van der
Vlugt, C., & Zwart, H. (2022). Society and synthetic
cells: A position paper by the Future Panel on Synthetic
Life. Rathenau Instituut.
2021
Iyer,
A., Baranov, M., Foster, A.
J., Chordia, S., Roelfes,
G., Vlijm, R., van den Bogaart,
G., & Poolman, B. (2021).
Chemogenetic Tags with Probe Exchange for Live-Cell
Fluorescence Microscopy. ACS chemical
biology, 16(5), 891-904. [acschembio.1c00100].
https://doi.org/10.1021/acschembio.1c00100
Mantovanelli,
L., Gaastra, B. F., & Poolman,
B. (2021). Fluorescence-based sensing of the
bioenergetic and physicochemical status of the cell. In M.
A. Model, & I. Levitan (Eds.), Current Topics in Membranes:
New Methods and Sensors for Membrane and Cell Volume Research
(Vol. 88, pp. 1-54). (Current Topics in Membranes). ACADEMIC PRESS
INC ELSEVIER SCIENCE. https://doi.org/10.1016/bs.ctm.2021.10.002
Åberg,
C., & Poolman, B. (2021). Glass-Like
Characteristics of Intracellular Motion in Human Cells.
Biophysical Journal, 120(11),
2355-2366. https://doi.org/10.1016/j.bpj.2021.04.011
Alvarez-Sieiro,
P., Sikkema, H. R., & Poolman,
B. (2021). Heterodimer Formation of the Homodimeric
ABC Transporter OpuA. International Journal of
Molecular Sciences, 22(11), [5912]. https://doi.org/10.3390/ijms22115912
Sikkema,
H. R., & Poolman, B. (2021). In
silico method for selecting residue pairs for single-molecule
microscopy and spectroscopy. Scientific
Reports, 11(1), [5756]. https://doi.org/10.1038/s41598-021-85003-0
Tran,
B. M., Prabha, H., Iyer, A., O'Byrne, C., Abee,
T., & Poolman, B. (2021). Measurement of
Protein Mobility in Listeria monocytogenes Reveals a Unique
Tolerance to Osmotic Stress and Temperature Dependence of
Diffusion. Frontiers in Microbiology,
12, [640149]. https://doi.org/10.3389/fmicb.2021.640149
Partipilo,
M., Ewins, E. J., Frallicciardi, J., Robinson,
T., Poolman, B., & Slotboom, D. J.
(2021). Minimal Pathway for the Regeneration of Redox
Cofactors. JACS Au, 1(12),
2280-2293. https://doi.org/10.1021/jacsau.1c00406
van
den Noort, M., de Boer, M., &
Poolman, B. (2021). Stability of Ligand-induced
Protein Conformation Influences Affinity in Maltose-binding
Protein. Journal of Molecular Biology,
433(15), [167036]. https://doi.org/10.1016/j.jmb.2021.167036
Plötz,
E., Schuurman-Wolters, G., Zijlstra, N., Jager, A.,
Griffith, D., Guskov, A., Gouridis, G., Poolman,
B., & Cordes, T. (2021). Structural
and biophysical characterization of the tandem substrate-binding
domains of the ABC importer GlnPQ. Open
Biology, 11(4), [200406]. https://doi.org/10.1098/rsob.200406
2020
Henderson,
R. K., de Valk, S. C., Poolman, B., & Mans, R.
(2021). Energy coupling of membrane transport and efficiency
of sucrose dissimilation in yeast. Metabolic
Engineering, 65, 243-254. https://doi.org/10.1016/j.ymben.2020.11.014
Pols,
T., Singh, S., Deelman-Driessen, C., Gaastra, B.
F., & Poolman, B. (2021). Enzymology
of the pathway for ATP production by arginine breakdown.
The FEBS Journal, 288(1), 293-309.
[febs.15337]. https://doi.org/10.1111/febs.15337
Liu,
B., Mavrova, S. N., van den Berg, J., Kristensen, S.
K., Mantovanelli, L., Veenhoff, L.
M., Poolman, B., & Boersma, A. J. (2020).
Erratum. ACS Sensors,
5(5), 1500-1500. https://doi.org/10.1021/acssensors.0c00744
Van't
Klooster, J. S., Bianchi, F., Doorn, R. B.,
Lorenzon, M., Lusseveld, J. H., Punter, C. M.,
& Poolman, B. (2020). Extracellular loops matter:
Subcellular location and function of the lysine transporter Lyp1
from Saccharomyces cerevisiae. The FEBS
Journal, 287(20), 4401-4414. [febs.15262]. https://doi.org/10.1111/febs.15262
Sikkema,
H. R., van den Noort, M., Rheinberger,
J., de Boer, M., Krepel, S. T.,
Schuurman-Wolters, G. K., Paulino, C., &
Poolman, B. (2020). Gating by ionic strength and
safety check by cyclic-di-AMP in the ABC transporter OpuA.
Science Advances, 6(47), [eabd7697].
https://doi.org/10.1126/sciadv.abd7697
Ruiz,
S. J., van 't Klooster, J. S., Bianchi,
F., & Poolman, B. (2020). Growth
Inhibition by Amino Acids in Saccharomyces cerevisiae.
Microorganisms, 9(1), 1-17.
[9010007]. https://doi.org/10.3390/microorganisms9010007
de
Boer, M., Cordes, T., & Poolman,
B. (2020). Kinetic Modelling of Transport Inhibition
by Substrates in ABC Importers. Journal of
Molecular Biology, 432(20), 5565-5576. https://doi.org/10.1016/j.jmb.2020.08.008
van
't Klooster, J. S., Cheng, T-Y., Sikkema, H.
R., Jeucken, A., Moody, D. B., &
Poolman, B. (2020). Membrane Lipid Requirements of the
Lysine Transporter Lyp1 from Saccharomyces cerevisiae.
Journal of Molecular Biology,
432(14), 4023-4031. https://doi.org/10.1016/j.jmb.2020.04.029
van
't Klooster, J. S., Cheng, T-Y., Sikkema, H.
R., Jeucken, A., Moody, B., &
Poolman, B. (2020). Periprotein lipidomes of
Saccharomyces cerevisiae provide a flexible environment for
conformational changes of membrane proteins.
eLife, 9, [e57003]. https://doi.org/10.7554/eLife.57003
Marinus,
N., Tahiri, N., Duca, M., Mouthaan, L. M. C.
M., Bianca, S., van den Noort,
M., Poolman, B., Witte, M.
D., & Minnaard, A. J. (2020).
Stereoselective Protection-Free Modification of
3-Keto-saccharides. Organic letters,
22(14), 5622-5626. https://doi.org/10.1021/acs.orglett.0c01986
Thomas,
C., Aller, S. G., Beis, K., Carpenter, E. P., Chang, G., Chen, L.,
Dassa, E., Dean, M., Duong Van Hoa, F., Ekiert, D., Ford, R.,
Gaudet, R., Gong, X., Holland, I. B., Huang, Y., Kahne, D. K.,
Kato, H., Koronakis, V., Koth, C. M., ... Tampé, R. (2020).
Structural and functional diversity calls for a new
classification of ABC transporters. FEBS
Letters, 594(23), 3767-3775.
[1873-3468.13935]. https://doi.org/10.1002/1873-3468.13935
2019
Syga,
L., De Vries, R. H., van Oosterhout, H., Bartelds, R.,
Boersma, A. J., Roelfes, G., & Poolman,
B. (2020). A trifunctional linker for palmitoylation
and peptide and protein localization in biological
membranes. ChemBioChem,
21(9), 1320-1328. https://doi.org/10.1002/cbic.201900655
Rempel,
I. L., Popken, P., Ghavami, A., Mishra, A., Hapsari,
R. A., Wolters, A. H. G., Veldsink, A.
C., Klaassens, M., Meinema, A. C., Poolman,
B., Giepmans, B. N. G., Onck, P.
R., Steen, A., & Veenhoff, L.
M. (2020). Flexible and Extended Linker Domains
Support Efficient Targeting of Heh2 to the Inner Nuclear
Membrane. Structure, 28(2),
185-195.e5. https://doi.org/10.1016/j.str.2019.11.003
Gabba,
M., & Poolman, B. (2020). Physiochemical
Modeling of Vesicle Dynamics upon Osmotic Upshift.
Biophysical Journal, 118(2),
435-447. [bpj.2019.11.3383]. https://doi.org/10.1016/j.bpj.2019.11.3383
Gabba,
M., Frallicciardi, J., van 't Klooster,
J., Henderson, R., Syga, Ł., Mans, R., van Maris, A. J.
A., & Poolman, B. (2020). Weak Acid
Permeation in Synthetic Lipid Vesicles and Across the Yeast Plasma
Membrane. Biophysical Journal,
118(2), 422-434. https://doi.org/10.1016/j.bpj.2019.11.3384
Price,
C. E., Branco Dos Santos, F., Hesseling, A., Uusitalo, J. J.,
Bachmann, H., Benavente, V., Goel, A., Berkhout, J., Bruggeman, F.
J., Marrink, S-J., Montalban-Lopez, M., de Jong,
A., Kok, J., Molenaar, D., Poolman,
B., Teusink, B., & Kuipers, O. P. (2019).
Adaption to glucose limitation is modulated by the pleotropic
regulator CcpA, independent of selection pressure strength.
BMC Evolutionary Biology, 19(1),
[15]. https://doi.org/10.1186/s12862-018-1331-x
Pols,
T., Sikkema, H. R., Gaastra, B. F.,
Frallicciardi, J., Śmigiel, W. M., Singh, S.,
& Poolman, B. (2019). A synthetic metabolic
network for physicochemical homeostasis. Nature
Communications, 10(1), [4239]. https://doi.org/10.1038/s41467-019-12287-2
Sikkema,
H. R., Gaastra, B. F., Pols, T., &
Poolman, B. (2019). Cell fueling and metabolic energy
conservation in synthetic cells.
ChemBioChem, 20(20 SI), 2581
–2592. https://doi.org/10.1002/cbic.201900398
de
Boer, M., Gkouridis, G., Vietrov, R., Begg, S. L.,
Schuurman-Wolters, G. K., Husada, F., Eleftheriadis, N.,
Poolman, B., McDevitt, C. A., & Cordes, T.
(2019). Conformational and dynamical plasticity in
substrate-binding proteins underlies selective transport in ABC
importers. eLife, 8,
[e44652]. https://doi.org/10.7554/eLife.44652
Liu,
B., Hasrat, Z., Poolman, B., & Boersma, A. J.
(2019). Decreased Effective Macromolecular Crowding in
Escherichia coli Adapted to Hyperosmotic Stress.
Journal of Bacteriology, 201(10),
[e00708-18]. https://doi.org/10.1128/JB.00708-18
Smigiel,
W. M., Lefrancois, P., & Poolman, B.
(2019). Physicochemical considerations for bottom-up
synthetic biology. Emerging topics in life
sciences, 3(5), 445-458. [ETLS20190017]. https://doi.org/10.1042/ETLS20190017
Bianchi,
F., Van't Klooster, J. S., Ruiz, S. J., & Poolman,
B. (2019). Regulation of Amino Acid Transport in
Saccharomyces cerevisiae. Microbiology and
Molecular Biology Reviews, 83(4), [e00024-19].
https://doi.org/10.1128/MMBR.00024-19
2018
Henderson,
R. K., Fendler, K., & Poolman, B. (2019).
Coupling efficiency of secondary active transporters.
Current Opinion in Biotechnology,
58, 62-71. https://doi.org/10.1016/j.copbio.2018.11.005
Marques,
W. L., Mans, R., Henderson, R. K., Marella, E. R., Horst, J. T.,
Hulster, E. D., Poolman, B., Daran, J-M., Pronk, J.
T., Gombert, A. K., & van Maris, A. J. A. (2018).
Combined engineering of disaccharide transport and
phosphorolysis for enhanced ATP yield from sucrose fermentation in
Saccharomyces cerevisiae. Metabolic
Engineering, 45, 121-133. https://doi.org/10.1016/j.ymben.2017.11.012
Lycklama
A Nijeholt, J. A., Vietrov, R., Schuurman-Wolters, G.
K., & Poolman, B. (2018). Energy
coupling efficiency in the Type I ABC transporter GlnPQ.
Journal of Molecular Biology,
430(6), 853-866. https://doi.org/10.1016/j.jmb.2018.02.001
Schavemaker,
P. E., Boersma, A. J., & Poolman, B. (2018).
How Important Is Protein Diffusion in Prokaryotes?
Frontiers in Molecular Biosciences,
5, [fmolb.2018.00093]. https://doi.org/10.3389/fmolb.2018.00093
Schavemaker,
P. E., & Poolman, B. (2018). (Membrane)
Protein Production in Context. Trends in
Biochemical Sciences, 43(11), 858-868. https://doi.org/10.1016/j.tibs.2018.08.009
Syga,
Ł., Spakman, D., Punter, C. M., &
Poolman, B. (2018). Method for immobilization of
living and synthetic cells for high-resolution imaging and
single-particle tracking. Scientific
Reports, 8(1), [13789]. https://doi.org/10.1038/s41598-018-32166-y
Bartelds,
R., Nematollahi, M. H., Pols, T., Stuart, M. C. A.,
Pardakhty, A., Asadikaram, G., & Poolman, B.
(2018). Niosomes, an alternative for liposomal
delivery. PLoS ONE, 13(4),
[e0194179]. https://doi.org/10.1371/journal.pone.0194179
Schuurman-Wolters,
G. K., de Boer, M., Pietrzyk, M. K.,
& Poolman, B. (2018). Protein linkers provide
limits on the domain interactions in the ABC importer GlnPQ and
determine the rate of transport. Journal of
Molecular Biology, 430(8), 1249-1262.
[j.jmb.2018.02.014]. https://doi.org/10.1016/j.jmb.2018.02.014
Bianchi,
F., Syga, Ł., Moiset, G., Spakman, D., Schavemaker, P.
E., Punter, C. M., Seinen, A-B., van Oijen, A.
M., Robinson, A., & Poolman, B. (2018).
Steric exclusion and protein conformation determine the
localization of plasma membrane transporters.
Nature Communications, 9(1), [501].
https://doi.org/10.1038/s41467-018-02864-2
Liu,
B., Mavrova, S. N., van den Berg, J., Kristensen, S.
K., Mantovanelli, L., Veenhoff, L.
M., Poolman, B., & Boersma, A. J. (2018).
The influence of fluorescent protein maturation on FRET
measurements in living cells. ACS
Sensors, 3(9), 1735-1742. https://doi.org/10.1021/acssensors.8b00473
2017
Cozzoli,
L., Gjonaj, L., Stuart, M. C. A., Poolman,
B., & Roelfes, G. (2018). Responsive
DNA G-quadruplex micelles. Chemical communications
(Cambridge, England), 54, 260-263. https://doi.org/10.1039/c7cc07899d
Franken,
L., Oostergetel, G., Pijning, T., Puri, P., Arkhipova,
V. I., Boekema, E., Poolman, B., & Guskov,
A. (2017). A general mechanism of ribosome
dimerization revealed by single-particle cryo-electron
microscopy. Nature Communications,
8, [722]. https://doi.org/10.1038/s41467-017-00718-x
Liu,
B., Åberg, C., van Eerden, F. J., Marrink,
S., Poolman, B., & Boersma, A. J. (2017).
Design and Properties of Genetically Encoded Probes for
Sensing Macromolecular Crowding. Biophysical
Journal, 112(9), 1929-1939. https://doi.org/10.1016/j.bpj.2017.04.004
Liu,
B., Poolman, B., & Boersma, A. J. (2017).
Ionic strength sensing in living cells. ACS
chemical biology, 12(10), 2510-2514.
[acschembio.7b00348]. https://doi.org/10.1021/acschembio.7b00348
Bartelds,
R., Barnoud, J., J. Boersma, A., J. Marrink, S.,
& Poolman, B. (2017). Lipid phase separation in
the presence of hydrocarbons in giant unilamellar vesicles.
AIMS Biophysics, 4(4), 528-542. https://doi.org/10.3934/biophy.2017.4.528
van
den Berg, J., Boersma, A. J., & Poolman, B.
(2017). Microorganisms maintain crowding homeostasis.
Nature Reviews Microbiology, 15,
309-318. [nrmicro.2017.17]. https://doi.org/10.1038/nrmicro.2017.17
Henderson,
R., & Poolman, B. (2017). Proton-solute
coupling mechanism of the maltose transporter from Saccharomyces
cerevisiae. Scientific Reports,
7(1), 1-12. [14375]. https://doi.org/10.1038/s41598-017-14438-1
Van
Meervelt, V., Soskine, M., Singh, S., Schuurman-Wolters,
G., Wijma, H. J., Poolman,
B., & Maglia, G. (2017). Real-time
conformational changes and controlled orientation of native
proteins inside a protein nanoreactor. Journal of
the American Chemical Society, 139(51),
18640-18646. [jacs.7b10106]. https://doi.org/10.1021/jacs.7b10106
Schavemaker,
P. E., Śmigiel, W. M., & Poolman, B. (2017).
Ribosome surface properties may impose limits on the nature
of the cytoplasmic proteome. eLife,
6, [eLife.30084]. https://doi.org/10.7554/eLife.30084
Poolman,
B., & Noireaux, V. (2017). Synthetic Biology: From
engineering living systems to the bottom=up construction of
synthetic cells. In J. van Doorn, J. Luirink, J. van der
Oost, B. Oudega, L. Robertson, H. Smit, P. Willemsen, & C.
Biemans (Eds.), MIGHTY MICROBES: THE AMAZING WORLD OF
MICROORGANISMS (1 ed., Vol. 1, pp. 215-217). Microcanon
Foundation.
2016
Scheepers,
G. H., Lycklama A Nijeholt, J. A., & Poolman, B.
(2016). An updated structural classification of
substrate-binding proteins. FEBS
Letters, 590(23), 4393-4401. https://doi.org/10.1002/1873-3468.12445
Popov-Čeleketić,
D., Bianchi, F., Ruiz, S. J., Meutiawati, F.,
& Poolman, B. (2016). A Plasma Membrane
Association Module in Yeast Amino Acid Transporters.
The Journal of Biological Chemistry,
291(31), 16024-16037. https://doi.org/10.1074/jbc.M115.706770
Bianchi,
F., Klooster, J. S. V. ., Ruiz, S. J., Luck,
K., Pols, T., Urbatsch, I. L., & Poolman, B.
(2016). Asymmetry in inward- and outward-affinity constant of
transport explain unidirectional lysine flux in Saccharomyces
cerevisiae. Scientific Reports,
6, 1-13. [31443]. https://doi.org/10.1038/srep31443
van
den Berg, J., & Poolman, B. (2016).
Compartmental Volume Regulation of E. Coli under Various
Growth Conditions. Biophysical
Journal, 110(3), 35A-35A. https://doi.org/10.1016/j.bpj.2015.11.259
Ruiz,
S. J., Schuurman-Wolters, G. K., & Poolman, B.
(2016). Crystal Structure of the Substrate-Binding Domain
from Listeria monocytogenes Bile-Resistance Determinant
BilE. Liquid Crystals, 6(12),
1-16. [cryst6120162]. https://doi.org/10.3390/cryst6120162
Maglia,
G., Soskine, M., Biesemans, A., Meervelt, V., &
Poolman, B. (2016). Nanopores with
internal protein adaptors. (Patent No.
WO2016166232). https://worldwide.espacenet.com/publicationDetails/originalDocument?CC=WO&NR=2016166232A1&KC=A1&FT=D&ND=3&date=20161020&DB=&locale=en_EP
van
den Berg, J., Galbiati, H., Rasmussen, A., Miller, S., &
Poolman, B. (2016). On the mobility, membrane location
and functionality of mechanosensitive channels in Escherichia
coli. Scientific Reports, 6,
[32709 ]. https://doi.org/10.1038/srep32709
van
Gijtenbeek, L. A., Robinson, A., van Oijen , A.,
Poolman, B., & Kok, J. (2016). On the Spatial
Organization of mRNA, Plasmids, and Ribosomes in a Bacterial Host
Overexpressing Membrane Proteins. PLoS
genetics, 12(12), 1-28. [e1006523]. https://doi.org/10.1371/journal.pgen.1006523
Boersma,
A., Liu, B., Åberg, C., & Poolman,
B. (2016). Quantification of Macromolecular Crowding
in Living Cells. Biophysical Journal,
110(3), 368A-368A. https://doi.org/10.1016/j.bpj.2015.11.1984
2015
Fulyani,
F., Schuurman-Wolters, G., Slotboom,
D-J., & Poolman, B. (2016). Relative
rates of amino acid import via the ABC transporter GlnPQ determine
the growth performance of Lactococcus lactis.
Journal of Bacteriology, 198(3),
477-485. [JB.00685-15]. https://doi.org/10.1128/JB.00685-15
Swier,
L. J. Y. M., Slotboom, D. J., &
Poolman, B. (2015). ABC importers. In A. George
(Ed.), ABC Transporters - 40 Years on (pp. 3-36). Springer
International Publishing. https://doi.org/10.1007/978-3-319-23476-2_1
Swier,
L. J. Y. M., Slotboom, D., & Poolman,
B. (2015). ABC transporters: A structure-function
perspective. In A. M. George (Ed.), ABC Transporters -
40 Years on (1 ed., Vol. 1, pp. 3-36). Springer Verlag.
Laba,
J. K., Steen, A., Popken, P., Chernova, A.,
Poolman, B., & Veenhoff, L. M. (2015).
Active Nuclear Import of Membrane Proteins Revisited.
Cells, 4(4), 653-673. https://doi.org/10.3390/cells4040653
Boersma,
A. J., Zuhorn, I. S., & Poolman, B.
(2015). A sensor for quantification of macromolecular
crowding in living cells. Nature
Methods, 12(3), 227-229. https://doi.org/10.1038/nmeth.3257
Gouridis,
G., Schuurman-Wolters, G., Ploetz, E., Husada, F.,
Vietrov, R., de Boer, M., Cordes,
T., & Poolman, B. (2015).
Conformational dynamics in substrate-binding domains
influences transport in the ABC importer GlnPQ.
Nature Structural & Molecular Biology,
22(1), 57-64. https://doi.org/10.1038/nsmb.2929
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Emergence of life: Physical chemistry changes the
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Engineering Escherichia coli for Functional Expression of
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Kralt,
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& Veenhoff, L. M. (2015). Intrinsically disordered
linker and plasma membrane-binding motif sort ist2 and ssy1 to
junctions. Traffic, 16(2),
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Goel,
A., Eckhardt, T. H., Puri, P., de Jong, A., Branco dos
Santos, F., Giera, M., Fusetti, F., de Vos, W. M., Kok,
J., Poolman, B., Molenaar, D., Kuipers,
O. P., & Teusink, B. (2015). Protein costs do not
explain evolution of metabolic strategies and regulation of
ribosomal content: does protein investment explain an anaerobic
bacterial Crabtree effect? Molecular
Microbiology, 97(1), 77-92. https://doi.org/10.1111/mmi.13012
Zollmann,
T., Moiset Coll, G., Tumulka, F., Tampé, R., Poolman,
B., & Abele, R. (2015). Single liposome analysis
of peptide translocation by the ABC transporter TAPL.
Proceedings of the National Academy of Sciences of the
United States of America, 112(7), 2046-2051.
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Popken,
P., Ghavami, A., Onck, P. R., Poolman,
B., & Veenhoff, L. M. (2015).
Size-dependent leak of soluble and membrane proteins through
the yeast nuclear pore complex. Molecular Biology
of the Cell, 26(7), 1386-1394. https://doi.org/10.1091/mbc.E14-07-1175
Husada,
F., Gouridis, G., Vietrov, R., Schuurman-Wolters, G.
K., Ploetz, E., de Boer, M., Poolman,
B., & Cordes, T. (2015). Watching
conformational dynamics of ABC transporters with single-molecule
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Bacillus subtilis spore protein SpoVAC functions as a
mechanosensitive channel. Molecular
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Moiset,
G., López, C. A., Bartelds, R., Syga, L., Rijpkema,
E., Cukkemane, A., Baldus, M., Poolman,
B., & Marrink, S. J. (2014).
Disaccharides Impact the Lateral Organization of Lipid
Membranes. Journal of the American Chemical
Society, 136(46), 16167-16175. https://doi.org/10.1021/ja505476c
Gul,
N., Linares, D. M., Ho, F. Y., & Poolman,
B. (2014). Evolved Escherichia coli Strains for
Amplified, Functional Expression of Membrane Proteins.
Journal of Molecular Biology,
426(1), 136-149. https://doi.org/10.1016/j.jmb.2013.09.009
Mika,
J., Schavemaker, P., Krasnikov, V., &
Poolman, B. (2014). Impact of osmotic stress on
protein diffusion in Lactococcus lactis. Molecular
Microbiology, 94(4), 857-870. https://doi.org/10.1111/mmi.12800
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Puri,
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C. A., Boekema, E. J., Kuipers, O. P.,
Kok, J., & Poolman, B. (2014).
Lactococcus lactis YfiA is necessary and sufficient for
ribosome dimerization. Molecular
Microbiology, 91(2), 394-407. https://doi.org/10.1111/mmi.12468
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J. E. (2014). Obituary: Wilhelmus Nicolaas Konings
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Puri,
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Regulation of acetate kinase isozymes and its importance for
mixed-acid fermentation in Lactococcus lactis.
Journal of Bacteriology, 196(7),
1386-1393. https://doi.org/10.1128/JB.01277-13
Franciosi,
L., Postma, D. S., van den Berge,
M., Govorukhina, N., Horvatovich, P.
L., Fusetti, F., Poolman, B., Lodewijk, M.
E., Timens, W., Bischoff, R., &
ten Hacken, N. (2014). Susceptibility to COPD:
Differential Proteomic Profiling after Acute Smoking.
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Puri,
P., Wetzel, C., Saffert, P., Gaston, K. W., Russel, S. P., Cordero
Varela, J. A., van der Vlies, P., Zhang, G., Limbach, P. A.,
Ignatova, Z., & Poolman, B. (2014).
Systematic identification of tRNAome and its dynamics in
Lactococcus lactis. Molecular
Microbiology, 93(5), 944-956. https://doi.org/10.1111/mmi.12710
Jansma,
M., Aalbers, F. S., & Poolman, B. (2014).
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2013
Moiset,
G., Cirac, A. D., Stuart, M. C. A., Marrink,
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Dual action of BPC194: a membrane active peptide killing
bacterial cells. PLoS ONE,
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& Poolman, B. (2013). Functional Diversity of
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Pathogenic Bacteria. Structure,
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transporter from Escherichia coli. Molecular
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Spitzer,
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prokaryotic cytoplasm? FEBS Letters,
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Remus,
D. M., Bongers, R. S., Meijerink, M., Fusetti, F., Poolman,
B., de Vos, P., Wells, J. M., Kleerebezem, M.,
& Bron, P. A. (2013). Impact of Lactobacillus plantarum
Sortase on Target Protein Sorting, Gastrointestinal Persistence,
and Host Immune Response Modulation. Journal of
Bacteriology, 195(3), 502-509. https://doi.org/10.1128/JB.01321-12
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den Bogaart, G., Meinema, A. C., Krasnikov, V.,
Veenhoff, L. M., & Poolman, B. (2013).
Nuclear transport factor directs localization of protein
synthesis during mitosis (Corrigendum; vol 11, pg 350,
2009). Nature Cell Biology,
15(4), 441. https://doi.org/10.1038/ncb2728
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