Poolman, Prof. Bert
It is one of the most fundamental questions in science: how can lifeless molecules come together to form a living cell? Bert Poolman, Professor of Biochemistry at the University of Groningen, has been working on this problem for over twenty years. He aims to understand life by trying to reconstruct it; he is building simplified artificial versions of biological systems that can be used as components for a synthetic cell.
Poolman was trained in bioenergetics and microbiology and moved to biochemistry and biophysics in later years. Central questions in his research are: How do molecules permeate biological membranes and how can one control solute fluxes? Throughout his career, he has made seminal contributions to the understanding of the dynamics and permeability of biological membranes and to the field of vectorial biochemistry, i.e. the role of electrochemical gradients in the fuelling and regulation of membrane transport. Poolman has a leading record in the energetics of membrane transport and cellular osmoregulation as well as the development of innovative technologies in membrane reconstitution.
In 2015 Poolman was awarded a European ERC Advanced Grant of 2,5 million euros for his project 'ABCVolume: The ABC of Cell Volume Regulation'. In 2024 he was awarded a European Synergy Grant of 5 million euros to design reaction networks for recycling of metabolic energy, production of cell building blocks and the construction of a minimal cell division system.
Poolman is program director of the Centre for Synthetic Biology. He has published over 270 articles in international peer-refereed scientific journals and supervised over 65 PhD students. He has been a member of the Royal Dutch Academy of Arts and Sciences (KNAW) since 2009.
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Publications
2025
Presutti,
C., Vreeker, E., Sasidharan, S.,
Ferdinando, Z., Stuart, M.,
Juhaniewicz-Dębińska, J., Maglia, G.,
Roos, W. H., & Poolman, B. (2025).
Balancing Permeability and Stability: A Study of Hybrid
Membranes for Synthetic Cells Using Lipids and PBd-b-PEO Block
Copolymers. Biomacromolecules, Article
4c01651. Advance online publication. https://doi.org/10.1021/acs.biomac.4c01651
2024
Heinen,
L., van den Noort, M., King, M. S., Kunji, E.
R. S., & Poolman, B. (2025). Synthetic
syntrophy for adenine nucleotide cross-feeding between
metabolically active nanoreactors. Nature
Nanotechnology, 20, 112-120. https://doi.org/10.1038/s41565-024-01811-1
Foster,
A. J., van den Noort, M., & Poolman,
B. (2024). Bacterial cell volume regulation and the
importance of cyclic di-AMP. Microbiology and
Molecular Biology Reviews, 88(2), Article
e0018123. https://doi.org/10.1128/mmbr.00181-23
Patiño-Ruiz,
M. F., Anshari, Z. R., Gaastra,
B., Slotboom, D. J., & Poolman,
B. (2024). Chemiosmotic nutrient transport in
synthetic cells powered by electrogenic antiport coupled to
decarboxylation. Nature
Communications, 15(1), Article 7976. https://doi.org/10.1038/s41467-024-52085-z
Coenradij,
J., Bailoni, E., & Poolman, B.
(2024). Construction of Out-of-Equilibrium Metabolic Networks
in Nano- and Micrometer-Sized Vesicles. Journal of
visualized experiments : JoVE, (206), Article 66627. https://doi.org/10.3791/66627
Linnik,
D., Maslov, I., Punter, C.
M., & Poolman, B. (2024). Dynamic
structure of E. coli cytoplasm: supramolecular complexes and cell
aging impact spatial distribution and mobility of proteins.
Communications biology, 7(1),
Article 508. https://doi.org/10.1038/s42003-024-06216-3
Szomek,
M., Akkerman, V., Lauritsen, L., Walther, H.-L., Juhl, A. D.,
Thaysen, K., Egebjerg, J. M., Covey, D. F., Lehmann, M., Wessig,
P., Foster, A. J., Poolman, B., Werner,
S., Schneider, G., Müller, P., & Wüstner, D. (2024).
Ergosterol promotes aggregation of natamycin in the yeast
plasma membrane. Biochimica et Biophysica Acta -
Biomembranes, 1866(7), Article 184350. https://doi.org/10.1016/j.bbamem.2024.184350
Nemchinova,
M., Schuurman-Wolters, G. K., Whittaker,
J. J., Arkhipova, V., Marrink, S. J.,
Poolman, B., & Guskov, A. (2024).
Exploring the Ligand Binding and Conformational Dynamics of
the Substrate-Binding Domain 1 of the ABC Transporter GlnPQ.
The Journal of Physical Chemistry B,
128(32), 7822-7832. https://doi.org/10.1021/acs.jpcb.4c02662
Karapanagioti,
F., Atlason, Ú. Á., Slotboom,
D. J., Poolman, B., & Obermaier,
S. (2024). Fitness landscape of substrate-adaptive
mutations in evolved amino acid-polyamine-organocation
transporters. eLife, 13,
Article RP93971. https://doi.org/10.7554/eLife.93971
Monterroso,
B., Margolin, W., Boersma, A. J., Rivas, G., Poolman,
B., & Zorrilla, S. (2024). Macromolecular
Crowding, Phase Separation, and Homeostasis in the Orchestration of
Bacterial Cellular Functions. Chemical
reviews, 124(4), 1899–1949. Article
3c00622. https://doi.org/10.1021/acs.chemrev.3c00622
Foster,
A. J., Li, H., Drougkas, P.,
Schuurman-Wolters, G. K., ten Kate, J., Paulino,
C., & Poolman, B. (2024).
Membrane-embedded CdaA is required for efficient synthesis of
second messenger cyclic di-AMP. Communications
biology, 7(1), Article 1710. https://doi.org/10.1038/s42003-024-07420-x
van
der Sleen, L., Stevens, J. A., Marrink,
S. J., Poolman, B., & Tych, K.
(2024). Probing the stability and interdomain interactions in
the ABC transporter OpuA using single-molecule optical
tweezers. Cell reports,
43(4), Article 114110. https://doi.org/10.1016/j.celrep.2024.114110
Bailoni,
E., Patiño-Ruiz, M. F., Stan, A. R.,
Schuurman-Wolters, G. K., Exterkate, M., Driessen, A.
J. M., & Poolman, B. (2024).
Synthetic Vesicles for Sustainable Energy Recycling and
Delivery of Building Blocks for Lipid Biosynthesis.
ACS Synthetic Biology, 13(5),
1549–1561. https://doi.org/10.1021/acssynbio.4c00073
Iyer,
A., Frallicciardi, J., le Paige, U. B. A.,
Narasimhan, S., Luo, Y., Alvarez Sieiro, P., Syga, L.,
van den Brekel, F., Minh Tran, B., Tjioe, R.,
Schuurman-Wolters, G., C A Stuart, M., Baldus,
M., van Ingen, H., & Poolman, B. (2024). The
structure and function of the bacterial osmotically inducible
protein Y. Journal of Molecular
Biology, 436(16), Article 168668. https://doi.org/10.1016/j.jmb.2024.168668
van
den Noort, M., Drougkas, P., Paulino,
C., & Poolman, B. (2024). The
substrate-binding domains of the osmoregulatory ABC importer OpuA
transiently interact. eLife,
12, Article 90996. https://doi.org/10.7554/eLife.90996
2023
Minh
Tran, B., Michiel Punter, C., Linnik,
D., Iyer, A., & Poolman, B.
(2024). Single-protein diffusion in the periplasm of
Escherichia coli. Journal of Molecular
Biology, 436(4), Article 168420. https://doi.org/10.1016/j.jmb.2023.168420
Bailoni,
E., Partipilo, M., Coenradij,
J., Grundel, D. A. J., Slotboom, D.
J., & Poolman, B. (2023). Minimal
Out-of-Equilibrium Metabolism for Synthetic Cells: A Membrane
Perspective. ACS Synthetic Biology,
12. https://doi.org/10.1021/acssynbio.3c00062
Poolman,
B. (2023). Physicochemical homeostasis in
bacteria. FEMS Microbiology Reviews,
47(4), Article fuad033. https://doi.org/10.1093/femsre/fuad033
Mantovanelli,
L., Linnik, D. S., Punter,
M., Kojakhmetov, H. J., Śmigiel, W.
M., & Poolman, B. (2023). Simulation-based
Reconstructed Diffusion unveils the effect of aging on protein
diffusion in Escherichia coli. PLoS Computational
Biology, 19(9), Article e1011093. https://doi.org/10.1371/journal.pcbi.1011093
Tran,
B. M., Linnik, D. S., Punter, C.
M., Śmigiel, W. M., Mantovanelli,
L., Iyer, A., O’Byrne, C., Abee, T.,
Johansson, J., & Poolman, B. (2023).
Super-resolving microscopy reveals the localizations and
movement dynamics of stressosome proteins in Listeria
monocytogenes. Communications biology,
6(1), Article 51. https://doi.org/10.1038/s42003-023-04423-y
2022
Bailoni,
E., & Poolman, B. (2022). ATP
Recycling Fuels Sustainable Glycerol 3-Phosphate Formation in
Synthetic Cells Fed by Dynamic Dialysis. ACS
Synthetic Biology, 11(7), 2348–2360.
Article 2c00075. https://doi.org/10.1021/acssynbio.2c00075
Frallicciardi,
J., Gabba, M., & Poolman, B. (2022).
Determining small-molecule permeation through lipid
membranes. Nature protocols,
17, 2620-2646. https://doi.org/10.1038/s41596-022-00734-2
Frallicciardi,
J., Melcr, J., Siginou, P.,
Marrink, S. J., & Poolman, B. (2022).
Membrane thickness, lipid phase and sterol type are
determining factors in the permeability of membranes to small
solutes. Nature Communications,
13(1), Article 1605. https://doi.org/10.1038/s41467-022-29272-x
Szomek,
M., Reinholdt, P., Walther, H. L., Scheidt, H. A., Müller,
P., Obermaier, S., Poolman, B., Kongsted,
J., & Wüstner, D. (2022). Natamycin sequesters
ergosterol and interferes with substrate transport by the lysine
transporter Lyp1 from yeast. Biochimica et
Biophysica Acta - Biomembranes, 1864(11),
Article 184012. https://doi.org/10.1016/j.bbamem.2022.184012
Smigiel,
W. M., Mantovanelli, L., Linnik, D.
S., Punter, M., Silberberg, J., Xiang, L., Xu,
K., & Poolman, B. (2022). Protein diffusion
in Escherichia coli cytoplasm scales with the mass ofthe complexes
and is location dependent. Science
Advances, 8(32), Article eabo5387. https://doi.org/10.1126/sciadv.abo5387
Aarts,
N., Bovenberg, R., Dogterom, M., van Est, R.,
Gerritsen, J., Macnaghten, P., Poolman, B., Robaey,
Z., Rasmussen, S., Ruivenkamp, G., Thole, E., Tremmel, G., van der
Vlugt, C., & Zwart, H. (2022). Society and synthetic
cells: A position paper by the Future Panel on Synthetic
Life. Rathenau Instituut.
2021
Iyer,
A., Baranov, M., Foster, A.
J., Chordia, S., Roelfes,
G., Vlijm, R., van den Bogaart,
G., & Poolman, B. (2021).
Chemogenetic Tags with Probe Exchange for Live-Cell
Fluorescence Microscopy. ACS chemical
biology, 16(5), 891-904. Article
acschembio.1c00100. https://doi.org/10.1021/acschembio.1c00100
Mantovanelli,
L., Gaastra, B. F., & Poolman,
B. (2021). Fluorescence-based sensing of the
bioenergetic and physicochemical status of the cell. In M.
A. Model, & I. Levitan (Eds.), Current Topics in Membranes:
New Methods and Sensors for Membrane and Cell Volume Research
(Vol. 88, pp. 1-54). (Current Topics in Membranes). ACADEMIC PRESS
INC ELSEVIER SCIENCE. https://doi.org/10.1016/bs.ctm.2021.10.002
Åberg,
C., & Poolman, B. (2021). Glass-Like
Characteristics of Intracellular Motion in Human Cells.
Biophysical Journal, 120(11),
2355-2366. https://doi.org/10.1016/j.bpj.2021.04.011
Alvarez-Sieiro,
P., Sikkema, H. R., & Poolman,
B. (2021). Heterodimer Formation of the Homodimeric
ABC Transporter OpuA. International Journal of
Molecular Sciences, 22(11), Article 5912. https://doi.org/10.3390/ijms22115912
Sikkema,
H. R., & Poolman, B. (2021). In
silico method for selecting residue pairs for single-molecule
microscopy and spectroscopy. Scientific
Reports, 11(1), Article 5756. https://doi.org/10.1038/s41598-021-85003-0
Tran,
B. M., Prabha, H., Iyer, A., O'Byrne, C., Abee,
T., & Poolman, B. (2021). Measurement of
Protein Mobility in Listeria monocytogenes Reveals a Unique
Tolerance to Osmotic Stress and Temperature Dependence of
Diffusion. Frontiers in Microbiology,
12, Article 640149. https://doi.org/10.3389/fmicb.2021.640149
Partipilo,
M., Ewins, E. J., Frallicciardi, J., Robinson,
T., Poolman, B., & Slotboom, D. J.
(2021). Minimal Pathway for the Regeneration of Redox
Cofactors. JACS Au, 1(12),
2280-2293. https://doi.org/10.1021/jacsau.1c00406
van
den Noort, M., de Boer, M., &
Poolman, B. (2021). Stability of Ligand-induced
Protein Conformation Influences Affinity in Maltose-binding
Protein. Journal of Molecular Biology,
433(15), Article 167036. https://doi.org/10.1016/j.jmb.2021.167036
Plötz,
E., Schuurman-Wolters, G., Zijlstra, N., Jager, A.,
Griffith, D., Guskov, A., Gouridis, G., Poolman,
B., & Cordes, T. (2021). Structural
and biophysical characterization of the tandem substrate-binding
domains of the ABC importer GlnPQ. Open
Biology, 11(4), Article 200406. https://doi.org/10.1098/rsob.200406
2020
Henderson,
R. K., de Valk, S. C., Poolman, B., & Mans, R.
(2021). Energy coupling of membrane transport and efficiency
of sucrose dissimilation in yeast. Metabolic
Engineering, 65, 243-254. https://doi.org/10.1016/j.ymben.2020.11.014
Pols,
T., Singh, S., Deelman-Driessen, C., Gaastra, B.
F., & Poolman, B. (2021). Enzymology
of the pathway for ATP production by arginine breakdown.
The FEBS Journal, 288(1), 293-309.
Article febs.15337. https://doi.org/10.1111/febs.15337
Liu,
B., Mavrova, S. N., van den Berg, J., Kristensen, S.
K., Mantovanelli, L., Veenhoff, L.
M., Poolman, B., & Boersma, A. J. (2020).
Erratum. ACS Sensors,
5(5), 1500-1500. https://doi.org/10.1021/acssensors.0c00744
Van't
Klooster, J. S., Bianchi, F., Doorn, R. B.,
Lorenzon, M., Lusseveld, J. H., Punter, C. M.,
& Poolman, B. (2020). Extracellular loops matter:
Subcellular location and function of the lysine transporter Lyp1
from Saccharomyces cerevisiae. The FEBS
Journal, 287(20), 4401-4414. Article
febs.15262. https://doi.org/10.1111/febs.15262
Sikkema,
H. R., van den Noort, M., Rheinberger,
J., de Boer, M., Krepel, S. T.,
Schuurman-Wolters, G. K., Paulino, C., &
Poolman, B. (2020). Gating by ionic strength and
safety check by cyclic-di-AMP in the ABC transporter OpuA.
Science Advances, 6(47), Article
eabd7697. https://doi.org/10.1126/sciadv.abd7697
Ruiz,
S. J., van 't Klooster, J. S., Bianchi,
F., & Poolman, B. (2020). Growth
Inhibition by Amino Acids in Saccharomyces cerevisiae.
Microorganisms, 9(1), 1-17. Article
9010007. https://doi.org/10.3390/microorganisms9010007
de
Boer, M., Cordes, T., & Poolman,
B. (2020). Kinetic Modelling of Transport Inhibition
by Substrates in ABC Importers. Journal of
Molecular Biology, 432(20), 5565-5576. https://doi.org/10.1016/j.jmb.2020.08.008
van
't Klooster, J. S., Cheng, T.-Y., Sikkema, H.
R., Jeucken, A., Moody, D. B., &
Poolman, B. (2020). Membrane Lipid Requirements of the
Lysine Transporter Lyp1 from Saccharomyces cerevisiae.
Journal of Molecular Biology,
432(14), 4023-4031. https://doi.org/10.1016/j.jmb.2020.04.029
van
't Klooster, J. S., Cheng, T.-Y., Sikkema, H.
R., Jeucken, A., Moody, B., &
Poolman, B. (2020). Periprotein lipidomes of
Saccharomyces cerevisiae provide a flexible environment for
conformational changes of membrane proteins.
eLife, 9, Article e57003. https://doi.org/10.7554/eLife.57003
Marinus,
N., Tahiri, N., Duca, M., Mouthaan, L. M. C.
M., Bianca, S., van den Noort,
M., Poolman, B., Witte, M.
D., & Minnaard, A. J. (2020).
Stereoselective Protection-Free Modification of
3-Keto-saccharides. Organic letters,
22(14), 5622-5626. https://doi.org/10.1021/acs.orglett.0c01986
Thomas,
C., Aller, S. G., Beis, K., Carpenter, E. P., Chang, G., Chen, L.,
Dassa, E., Dean, M., Duong Van Hoa, F., Ekiert, D., Ford, R.,
Gaudet, R., Gong, X., Holland, I. B., Huang, Y., Kahne, D. K.,
Kato, H., Koronakis, V., Koth, C. M., ... Tampé, R. (2020).
Structural and functional diversity calls for a new
classification of ABC transporters. FEBS
Letters, 594(23), 3767-3775. Article
1873-3468.13935. https://doi.org/10.1002/1873-3468.13935
2019
Syga,
L., De Vries, R. H., van Oosterhout, H., Bartelds, R.,
Boersma, A. J., Roelfes, G., & Poolman,
B. (2020). A trifunctional linker for palmitoylation
and peptide and protein localization in biological
membranes. ChemBioChem,
21(9), 1320-1328. https://doi.org/10.1002/cbic.201900655
Rempel,
I. L., Popken, P., Ghavami, A., Mishra, A., Hapsari,
R. A., Wolters, A. H. G., Veldsink, A.
C., Klaassens, M., Meinema, A. C., Poolman,
B., Giepmans, B. N. G., Onck, P.
R., Steen, A., & Veenhoff, L.
M. (2020). Flexible and Extended Linker Domains
Support Efficient Targeting of Heh2 to the Inner Nuclear
Membrane. Structure, 28(2),
185-195.e5. https://doi.org/10.1016/j.str.2019.11.003
Gabba,
M., & Poolman, B. (2020). Physiochemical
Modeling of Vesicle Dynamics upon Osmotic Upshift.
Biophysical Journal, 118(2),
435-447. Article bpj.2019.11.3383. https://doi.org/10.1016/j.bpj.2019.11.3383
Gabba,
M., Frallicciardi, J., van 't Klooster,
J., Henderson, R., Syga, Ł., Mans, R., van Maris, A. J.
A., & Poolman, B. (2020). Weak Acid
Permeation in Synthetic Lipid Vesicles and Across the Yeast Plasma
Membrane. Biophysical Journal,
118(2), 422-434. https://doi.org/10.1016/j.bpj.2019.11.3384
Price,
C. E., Branco Dos Santos, F., Hesseling, A., Uusitalo, J. J.,
Bachmann, H., Benavente, V., Goel, A., Berkhout, J., Bruggeman, F.
J., Marrink, S.-J., Montalban-Lopez, M., de
Jong, A., Kok, J., Molenaar, D., Poolman,
B., Teusink, B., & Kuipers, O. P. (2019).
Adaption to glucose limitation is modulated by the pleotropic
regulator CcpA, independent of selection pressure strength.
BMC Evolutionary Biology, 19(1),
Article 15. https://doi.org/10.1186/s12862-018-1331-x
Pols,
T., Sikkema, H. R., Gaastra, B. F.,
Frallicciardi, J., Śmigiel, W. M., Singh, S.,
& Poolman, B. (2019). A synthetic metabolic
network for physicochemical homeostasis. Nature
Communications, 10(1), Article 4239. https://doi.org/10.1038/s41467-019-12287-2
Sikkema,
H. R., Gaastra, B. F., Pols, T., &
Poolman, B. (2019). Cell fueling and metabolic energy
conservation in synthetic cells.
ChemBioChem, 20(20 SI),
2581–2592. https://doi.org/10.1002/cbic.201900398
de
Boer, M., Gkouridis, G., Vietrov, R., Begg, S. L.,
Schuurman-Wolters, G. K., Husada, F., Eleftheriadis, N.,
Poolman, B., McDevitt, C. A., & Cordes, T.
(2019). Conformational and dynamical plasticity in
substrate-binding proteins underlies selective transport in ABC
importers. eLife, 8, Article
e44652. https://doi.org/10.7554/eLife.44652
Liu,
B., Hasrat, Z., Poolman, B., & Boersma, A. J.
(2019). Decreased Effective Macromolecular Crowding in
Escherichia coli Adapted to Hyperosmotic Stress.
Journal of Bacteriology, 201(10),
Article e00708-18. https://doi.org/10.1128/JB.00708-18
Smigiel,
W. M., Lefrancois, P., & Poolman, B.
(2019). Physicochemical considerations for bottom-up
synthetic biology. Emerging topics in life
sciences, 3(5), 445-458. Article ETLS20190017.
https://doi.org/10.1042/ETLS20190017
Bianchi,
F., Van't Klooster, J. S., Ruiz, S. J., & Poolman,
B. (2019). Regulation of Amino Acid Transport in
Saccharomyces cerevisiae. Microbiology and
Molecular Biology Reviews, 83(4), Article
e00024-19. https://doi.org/10.1128/MMBR.00024-19
2018
Henderson,
R. K., Fendler, K., & Poolman, B. (2019).
Coupling efficiency of secondary active transporters.
Current Opinion in Biotechnology,
58, 62-71. https://doi.org/10.1016/j.copbio.2018.11.005
Marques,
W. L., Mans, R., Henderson, R. K., Marella, E. R., Horst, J. T.,
Hulster, E. D., Poolman, B., Daran, J.-M., Pronk, J.
T., Gombert, A. K., & van Maris, A. J. A. (2018).
Combined engineering of disaccharide transport and
phosphorolysis for enhanced ATP yield from sucrose fermentation in
Saccharomyces cerevisiae. Metabolic
Engineering, 45, 121-133. https://doi.org/10.1016/j.ymben.2017.11.012
Lycklama
A Nijeholt, J. A., Vietrov, R., Schuurman-Wolters, G.
K., & Poolman, B. (2018). Energy
coupling efficiency in the Type I ABC transporter GlnPQ.
Journal of Molecular Biology,
430(6), 853-866. https://doi.org/10.1016/j.jmb.2018.02.001
Schavemaker,
P. E., Boersma, A. J., & Poolman, B. (2018).
How Important Is Protein Diffusion in Prokaryotes?
Frontiers in Molecular Biosciences,
5, Article fmolb.2018.00093. https://doi.org/10.3389/fmolb.2018.00093
Schavemaker,
P. E., & Poolman, B. (2018). (Membrane)
Protein Production in Context. Trends in
Biochemical Sciences, 43(11), 858-868. https://doi.org/10.1016/j.tibs.2018.08.009
Syga,
Ł., Spakman, D., Punter, C. M., &
Poolman, B. (2018). Method for immobilization of
living and synthetic cells for high-resolution imaging and
single-particle tracking. Scientific
Reports, 8(1), Article 13789. https://doi.org/10.1038/s41598-018-32166-y
Bartelds,
R., Nematollahi, M. H., Pols, T., Stuart, M. C. A.,
Pardakhty, A., Asadikaram, G., & Poolman, B.
(2018). Niosomes, an alternative for liposomal
delivery. PLoS ONE, 13(4),
Article e0194179. https://doi.org/10.1371/journal.pone.0194179
Schuurman-Wolters,
G. K., de Boer, M., Pietrzyk, M. K.,
& Poolman, B. (2018). Protein linkers provide
limits on the domain interactions in the ABC importer GlnPQ and
determine the rate of transport. Journal of
Molecular Biology, 430(8), 1249-1262. Article
j.jmb.2018.02.014. https://doi.org/10.1016/j.jmb.2018.02.014
Bianchi,
F., Syga, Ł., Moiset, G., Spakman, D., Schavemaker, P.
E., Punter, C. M., Seinen, A.-B., van Oijen, A.
M., Robinson, A., & Poolman, B. (2018).
Steric exclusion and protein conformation determine the
localization of plasma membrane transporters.
Nature Communications, 9(1), Article
501. https://doi.org/10.1038/s41467-018-02864-2
Liu,
B., Mavrova, S. N., van den Berg, J., Kristensen, S.
K., Mantovanelli, L., Veenhoff, L.
M., Poolman, B., & Boersma, A. J. (2018).
The influence of fluorescent protein maturation on FRET
measurements in living cells. ACS
Sensors, 3(9), 1735-1742. https://doi.org/10.1021/acssensors.8b00473
2017
Cozzoli,
L., Gjonaj, L., Stuart, M. C. A., Poolman,
B., & Roelfes, G. (2018). Responsive
DNA G-quadruplex micelles. Chemical communications
(Cambridge, England), 54, 260-263. https://doi.org/10.1039/c7cc07899d
Franken,
L., Oostergetel, G., Pijning, T., Puri, P., Arkhipova,
V. I., Boekema, E., Poolman, B., & Guskov,
A. (2017). A general mechanism of ribosome
dimerization revealed by single-particle cryo-electron
microscopy. Nature Communications,
8, Article 722. https://doi.org/10.1038/s41467-017-00718-x
Liu,
B., Åberg, C., van Eerden, F. J., Marrink,
S., Poolman, B., & Boersma, A. J. (2017).
Design and Properties of Genetically Encoded Probes for
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Liu,
B., Poolman, B., & Boersma, A. J. (2017).
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chemical biology, 12(10), 2510-2514. Article
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Bartelds,
R., Barnoud, J., J. Boersma, A., J. Marrink, S.,
& Poolman, B. (2017). Lipid phase separation in
the presence of hydrocarbons in giant unilamellar vesicles.
AIMS Biophysics, 4(4), 528-542. https://doi.org/10.3934/biophy.2017.4.528
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den Berg, J., Boersma, A. J., & Poolman, B.
(2017). Microorganisms maintain crowding homeostasis.
Nature Reviews Microbiology, 15,
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Henderson,
R., & Poolman, B. (2017). Proton-solute
coupling mechanism of the maltose transporter from Saccharomyces
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Van
Meervelt, V., Soskine, M., Singh, S., Schuurman-Wolters,
G., Wijma, H. J., Poolman,
B., & Maglia, G. (2017). Real-time
conformational changes and controlled orientation of native
proteins inside a protein nanoreactor. Journal of
the American Chemical Society, 139(51),
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Schavemaker,
P. E., Śmigiel, W. M., & Poolman, B. (2017).
Ribosome surface properties may impose limits on the nature
of the cytoplasmic proteome. eLife,
6, Article eLife.30084. https://doi.org/10.7554/eLife.30084
Poolman,
B., & Noireaux, V. (2017). Synthetic Biology: From
engineering living systems to the bottom=up construction of
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2016
Scheepers,
G. H., Lycklama A Nijeholt, J. A., & Poolman, B.
(2016). An updated structural classification of
substrate-binding proteins. FEBS
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Popov-Čeleketić,
D., Bianchi, F., Ruiz, S. J., Meutiawati, F.,
& Poolman, B. (2016). A Plasma Membrane
Association Module in Yeast Amino Acid Transporters.
The Journal of Biological Chemistry,
291(31), 16024-16037. https://doi.org/10.1074/jbc.M115.706770
Bianchi,
F., Klooster, J. S. V. ., Ruiz, S. J., Luck,
K., Pols, T., Urbatsch, I. L., & Poolman, B.
(2016). Asymmetry in inward- and outward-affinity constant of
transport explain unidirectional lysine flux in Saccharomyces
cerevisiae. Scientific Reports,
6, 1-13. Article 31443. https://doi.org/10.1038/srep31443
van
den Berg, J., & Poolman, B. (2016).
Compartmental Volume Regulation of E. Coli under Various
Growth Conditions. Biophysical
Journal, 110(3), 35A-35A. https://doi.org/10.1016/j.bpj.2015.11.259
Ruiz,
S. J., Schuurman-Wolters, G. K., & Poolman, B.
(2016). Crystal Structure of the Substrate-Binding Domain
from Listeria monocytogenes Bile-Resistance Determinant
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Maglia,
G., Soskine, M., Biesemans, A., Meervelt, V., &
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van
den Berg, J., Galbiati, H., Rasmussen, A., Miller, S., &
Poolman, B. (2016). On the mobility, membrane location
and functionality of mechanosensitive channels in Escherichia
coli. Scientific Reports, 6,
Article 32709 . https://doi.org/10.1038/srep32709
van
Gijtenbeek, L. A., Robinson, A., van Oijen , A.,
Poolman, B., & Kok, J. (2016). On the Spatial
Organization of mRNA, Plasmids, and Ribosomes in a Bacterial Host
Overexpressing Membrane Proteins. PLoS
genetics, 12(12), 1-28. Article e1006523. https://doi.org/10.1371/journal.pgen.1006523
Boersma,
A., Liu, B., Åberg, C., & Poolman,
B. (2016). Quantification of Macromolecular Crowding
in Living Cells. Biophysical Journal,
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2015
Fulyani,
F., Schuurman-Wolters, G., Slotboom,
D.-J., & Poolman, B. (2016). Relative
rates of amino acid import via the ABC transporter GlnPQ determine
the growth performance of Lactococcus lactis.
Journal of Bacteriology, 198(3),
477-485. Article JB.00685-15. https://doi.org/10.1128/JB.00685-15
Swier,
L. J. Y. M., Slotboom, D., & Poolman,
B. (2015). ABC Importers. In A. M. George
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Laba,
J. K., Steen, A., Popken, P., Chernova, A.,
Poolman, B., & Veenhoff, L. M. (2015).
Active Nuclear Import of Membrane Proteins Revisited.
Cells, 4(4), 653-673. https://doi.org/10.3390/cells4040653
Boersma,
A. J., Zuhorn, I. S., & Poolman, B.
(2015). A sensor for quantification of macromolecular
crowding in living cells. Nature
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Gouridis,
G., Schuurman-Wolters, G., Ploetz, E., Husada, F.,
Vietrov, R., de Boer, M., Cordes,
T., & Poolman, B. (2015).
Conformational dynamics in substrate-binding domains
influences transport in the ABC importer GlnPQ.
Nature Structural & Molecular Biology,
22(1), 57-64. https://doi.org/10.1038/nsmb.2929
Spitzer,
J., Pielak, G. J., & Poolman, B. (2015).
Emergence of life: Physical chemistry changes the
paradigm. Biology Direct,
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F. Y., & Poolman, B. (2015).
Engineering Escherichia coli for Functional Expression of
Membrane Proteins. Methods in
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Driessen,
A. J. M., & Poolman, B. (2015). In
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Extremophiles, 19, 233-234. https://doi.org/10.1007/s00792-015-0736-3
Kralt,
A., Carretta, M., Mari, M., Reggiori,
F., Steen, A., Poolman, B.,
& Veenhoff, L. M. (2015). Intrinsically disordered
linker and plasma membrane-binding motif sort ist2 and ssy1 to
junctions. Traffic, 16(2),
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Goel,
A., Eckhardt, T. H., Puri, P., de Jong, A., Branco dos
Santos, F., Giera, M., Fusetti, F., de Vos, W. M., Kok,
J., Poolman, B., Molenaar, D., Kuipers,
O. P., & Teusink, B. (2015). Protein costs do not
explain evolution of metabolic strategies and regulation of
ribosomal content: does protein investment explain an anaerobic
bacterial Crabtree effect? Molecular
Microbiology, 97(1), 77-92. https://doi.org/10.1111/mmi.13012
Zollmann,
T., Moiset Coll, G., Tumulka, F., Tampé, R., Poolman,
B., & Abele, R. (2015). Single liposome analysis
of peptide translocation by the ABC transporter TAPL.
Proceedings of the National Academy of Sciences of the
United States of America, 112(7), 2046-2051.
https://doi.org/10.1073/pnas.1418100112
Popken,
P., Ghavami, A., Onck, P. R., Poolman,
B., & Veenhoff, L. M. (2015).
Size-dependent leak of soluble and membrane proteins through
the yeast nuclear pore complex. Molecular Biology
of the Cell, 26(7), 1386-1394. https://doi.org/10.1091/mbc.E14-07-1175
Husada,
F., Gouridis, G., Vietrov, R., Schuurman-Wolters, G.
K., Ploetz, E., de Boer, M., Poolman,
B., & Cordes, T. (2015). Watching
conformational dynamics of ABC transporters with single-molecule
tools. Biochemical Society
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2014
Velasquez
Guzman, J., Schuurman-Wolters, G., Birkner, J.
P., Abee, T., & Poolman, B. (2014).
Bacillus subtilis spore protein SpoVAC functions as a
mechanosensitive channel. Molecular
Microbiology. https://doi.org/10.1111/mmi.12591
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