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Over onsFaculty of Science and EngineeringOnderzoekGRIPAnalytical Biochemistry

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Analytics for Biologics – A4B – 15 PhD positions in a multidisciplinary research training program in qualitative and quantitative analysis and purification of therapeutic protein species.

The graduate school Analytics for Biologics (A4B) is offering 15 PhD positions with the aim to produce the much-needed specialists in field of production, down-stream processing and analysis of therapeutic proteins (TP). This Marie Sklodowska-Curie Innovative Training Network (ITN) is composed of 11 European academic and industrial institutions and provides the optimal scientific network for training schools and individual research projects toward a common goal.

Context: The market for therapeutic Proteins (TPs) is rapidly growing, causing a high demand in specialists in the field of protein production and analysis, which currently cannot be met by individual European universities. Production of TPs is associated with the generation of a mixture of very similar TP species (TP-S). Some of them are poorly effective or even harmful, with side effects up to life threatening allergic shocks. Since protein species are coded by the same gene and thereby chemically often very similar, their separation is difficult, making their (quantitative and qualitative) analysis one of the most challenging problems in analytical chemistry. A4B aims, therefore, at the development of methods for effective removal of TP-S with improved purification methods and the development of respective analytical tools to qualitatively and quantitatively detect the diverse TP-S. We will employ and improve a bioanalytical toolbox focusing on the identity and chemical composition of individual TP-S including post-translational modifications (PTMs) using high-end electrophoretic, liquid chromatographic (LC), and mass spectrometric (MS) approaches in combination with bioinformatics. The developed methods will be tested for their applicability in improving TP manufacturing, in pharmacokinetics and in protein metabolism.

Within her/his respective project, each PhD student will receive a background in production, analysis and economic exploitation of TPs, and training in relevant soft skills. This training will be provided during dedicated Training Schools, soft skills training sessions and a number of workshops to be hosted at the different partner institutions.

A mobility rule applies, notably applicants must not have spent more than 12 months in the country of the host institution within the 3 years immediately prior to their recruitment.

To apply, applicants must:

- have a Master’s degree (or equivalent) with outstanding performance

- be in the first four years of their research careers and have not been awarded a doctoral degree

- have excellent English skills

- send a detailed Curriculum Vitae

- send a personal statement describing interests, confirming readiness to move to the host institution and awareness of mobility opportunities within this 4-year PhD program

- provide two academic references

- indicate which project(s) she/he is applying for

- please find more details about the Research Group at the University of Groningen hosting ESRs 9 and 10 at our homepage: www.biomac.nl )

              ESR1: Fast quantification of intact TP-S

              ESR2: Improvement of liquid chromatography for analysis and purification of TP-S via rational protein purification parameter screening and sample displacement chromatography

ESR3: Algorithms for quantitative TP-S analytics

              ESR4: Development and use of monoclonal affinity proteins

              ESR5: New methods for the mass spectrometric glycosylation analysis of TP-S

              ESR6: Separation and mass spectrometric characterization of intact TP-S including conformers and complexes

ESR7: Comprehensive characterization of TP-S by top-down mass spectrometry (TDMS) and its expansion to large proteins

ESR 8: Automatable and high throughput techniques to determine site specificity and quantification of N and O glycan profiles of EPO and TNF-AB

ESR 9, 10 and 15: Pharmacokinetics and Metabolism: Analysis of TP species in complex matrices

ESR11: Protein quantification by isotope labeling

              ESR12: Continuous separation of recombinant antibodies by non-chromatography methods

ESR13: Improvement of the production process

ESR14: Development of Multiple Reaction Monitoring (MRM) / Data-Independent-Acquisition (DIA) for protein quantification

Laatst gewijzigd:24 juli 2017 10:35