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founded in 1614 - top 100 university
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Experimental Hematology
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Protocols
protocols
general lab safety rules
Safety issues regarding retro/lenti transductions
generation of lentiviral particles
transduction protocol for human CB CD34+ cells
transduction protocol for AML CD34+ cells
LTC-IC
long-term MS5 cocultures using (transduced) AML CD34+ cells
long-term MS5 cocultures using transduced (CB) CD34+ cells
CFC assays
cytokine-driven liquid culture differentiation assays
mini-midi-maxi-mega
generation of stable PG13 retro-producer cell lines
Q-PCR
isolation of RNA
Western blot procedures
Rubidium Chloride method for competent E.Coli
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