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Fraaije, Prof. Marco

Marco Fraaije
Marco Fraaije

Prof. Marco W. Fraaije studied Molecular Science at Wageningen University & Research, where he also gained his PhD. He was then a postdoctoral researcher at the Department of Genetics and Microbiology at the University of Pavia (Italy) and has worked at the University of Groningen since 1999. When he was an assistant professor, he led several studies centred around discovering new biocatalysts (enzymes) and researching their function.

At present, he is Professor of Molecular Enzymology. In 2016, he was awarded a Vici research grant and in 2018, he received the BIOCAT Science Award for his academic contribution to the biotechnology research field. Fraaije has published hundreds of articles, with a main focus on biocatalysis and enzyme engineering. His research revolves around discovering and designing new enzymes, focusing particularly on flavine adenine dinucleotide enzymes: those that use vitamin B2 to function.

In addition to the academic applications of his research, Fraaije is also building a bridge between the worlds of academia and industry. For example, he is the co-founder and current advisor of start-up company Gecco Biotech. Additionally, he has coordinated several EU-funded initiatives, among which the ROBOX project. This project is mainly aimed at designing robust enzymes that can be used in oxidation processes. These enzymes can replace chemical reactions that are currently being used in production processes. The aim is to reduce the need to use precious metals and toxic substances. Furthermore, his research group is working on computer-based engineering methods to adjust enzymes quickly for biotechnological applications.


Guo, Y., Ashley, B., Marić, I., Saifuddin, M., Dunleavy, T., Onet, A., & Fraaije, M. W. (2024). Biocatalytic Oxidative Amination of para-Substituted Phenols. ChemCatChem, Article e202400277. Advance online publication.
Russo, S., Rozeboom, H. J., Wijma, H. J., Poelarends, G. J., & Fraaije, M. W. (2024). Biochemical, kinetic, and structural characterization of a Bacillus tequilensis nitroreductase. The FEBS Journal, Article 17210. Advance online publication.
Santema, L. L., Rotilio, L., Xiang, R., Tjallinks, G., Guallar, V., Mattevi, A., & Fraaije, M. W. (2024). Discovery and biochemical characterization of thermostable glycerol oxidases. Applied Microbiology and Biotechnology, 108(1), 1-14. Article 61.
Lee, M., & Fraaije, M. W. (2024). Equipping Saccharomyces cerevisiae with an Additional Redox Cofactor Allows F 420-Dependent Bioconversions in Yeast. ACS Synthetic Biology, 13(3), 921–929.
Yang, G., Pećanac, O., Wijma, H. J., Rozeboom, H. J., de Gonzalo, G., Fraaije, M. W., & Mascotti, M. L. (2024). Evolution of the catalytic mechanism at the dawn of the Baeyer-Villiger monooxygenases. Cell reports, 43(5), Article 114130.
Boverio, A., Jamil, N., Mannucci, B., Mascotti, M. L., Fraaije, M. W., & Mattevi, A. (2024). Structure, mechanism, and evolution of the last step in vitamin C biosynthesis. Nature Communications, 15(1), Article 4158.
Russo, S., Prats Luján, A., Fraaije, M., & Poelarends, G. J. (2024). Synthesis of Pharmaceutically Relevant Arylamines Enabled by a Nitroreductase from Bacillus tequilensis. ChemBioChem, 25(10), Article e202300846.
Fraaije, M. W., & Tong, Y. (2024). Variant flavoproteins comprising covalently tethered flavin cofactor, and means and methods related thereto. (Patent No. WO2024136655).


Santema, L. L., Basile, L., Binda, C., & Fraaije, M. W. (2024). Discovery and structural characterization of a thermostable bacterial monoamine oxidase. The FEBS Journal, 291(5), 849-864.
Rotilio, L., Boverio, A., Nguyen, Q.-T., Mannucci, B., Fraaije, M., & Mattevi, A. (2023). A biosynthetic aspartate N-hydroxylase performs successive oxidations by holding intermediates at a site away from the catalytic center. The Journal of Biological Chemistry, 299(7), Article 104904.
Arentshorst, M., Kooloth Valappil, P., Mózsik, L., Regensburg-Tuïnk, T. J. G., Seekles, S., Tjallinks, G., Fraaije, M., Visser, J., & Ram, A. F. J. (2023). A CRISPR/Cas9-based multicopy integration system for protein production in Aspergillus niger. The FEBS Journal, 5127-5140. Article 16891.
Marić, I., Guo, Y., Fürst, M. J. L. J., Van Aelst, K., Van den Bosch, S., De Simone, M., Martins, L. O., Sels, B. F., & Fraaije, M. W. (2023). A One-Pot, Whole-Cell Biocatalysis Approach for Vanillin Production using Lignin Oil. Advanced Synthesis and Catalysis, 365(22), 3987-3995.
Boverio, A., van Beek, H. L., Savino, S., Ranoux, A., Huijgen, W. J. J., Raaijmakers, H. W. C., Fraaije, M. W., & Lončar, N. (2023). Biochemical and Structural Characterization of a Uronic Acid Oxidase from Citrus sinensis. ChemCatChem, 15(21), Article e202300847.
Tong, Y., Rozeboom, H. J., Loonstra, M. R., Wijma, H. J., & Fraaije, M. W. (2023). Characterization of two bacterial multi-flavinylated proteins harboring multiple covalent flavin cofactors. BBA Advances, 4, Article 100097.
Tjallinks, G., Boverio, A., Jager, A. W., Kaya, S. G., Mattevi, A., & Fraaije, M. W. (2023). Efficient Oxidation of 5-Hydroxymethylfurfural Using a Flavoprotein Oxidase from the Honeybee Apis mellifera. ChemBioChem, 24(24), Article e202300588.
Bailleul, G., Yang, G., Nicoll, C. R., Mattevi, A., Fraaije, M. W., & Mascotti, M. L. (2023). Evolution of enzyme functionality in the flavin-containing monooxygenases. Nature Communications, 14(1), Article 1042.
Tong, Y., Kaya, S. G., Russo, S., Rozeboom, H. J., Wijma, H. J., & Fraaije, M. W. (2023). Fixing Flavins: Hijacking a Flavin Transferase for Equipping Flavoproteins with a Covalent Flavin Cofactor. Journal of the American Chemical Society, 145(49), 27140–27148.
Yang, G., Wijma, H. J., Rozeboom, H. J., Mascotti, M. L., & Fraaije, M. W. (2023). Identification and characterization of archaeal and bacterial F420-dependent thioredoxin reductases. The FEBS Journal, 290(19), 4777-4791.
Nicoll, C. R., Massari, M., Fraaije, M. W., Mascotti, M. L., & Mattevi, A. (2023). Impact of ancestral sequence reconstruction on mechanistic and structural enzymology. Current Opinion in Structural Biology, 82, Article 102669.
Guo, Y., Alvigini, L., Saifuddin, M., Ashley, B., Trajkovic, M., Alonso-Cotchico, L., Mattevi, A., & Fraaije, M. W. (2023). One-pot biocatalytic synthesis of rac-syringaresinol from a lignin-derived phenol. ACS Catalysis, 13(22), 14639-14649.
Tjallinks, G., Boverio, A., Maric, I., Rozeboom, H., Arentshorst, M., Visser, J., Ram, A. F. J., Mattevi, A., & Fraaije, M. W. (2023). Structure elucidation and characterization of patulin synthase, insights into the formation of a fungal mycotoxin. The FEBS Journal, 290(21), 5114-5126. Article 16804.
Eggerichs, D., Weindorf, N., Mascotti, M. L., Welzel, N., Fraaije, M. W., & Tischler, D. (2023). Vanillyl alcohol oxidase from Diplodia corticola: Residues Ala420 and Glu466 allow for efficient catalysis of syringyl derivatives. The Journal of Biological Chemistry, 299(7), Article 104898.


de Gonzalo, G., Loncar, N., & Fraaije, M. (2024). Sulphoxidation reactions catalysed by the Baeyer-Villiger monooxygenase OTEMO from Pseudomonas putida ATCC 17453. Biocatalysis and Biotransformation, 42(1), 77-84. Article 2113519.
Boverio, A., Widodo, W. S., Santema, L. L., Rozeboom, H. J., Xiang, R., Guallar, V., Mattevi, A., & Fraaije, M. W. (2023). Structural Elucidation and Engineering of a Bacterial Carbohydrate Oxidase. Biochemistry, 62(2), 429-436.
Partipilo, M., Yang, G., Mascotti, L., Slotboom, D., & Fraaije, M. (2022). A conserved sequence motif in the E. coli soluble FAD-containing pyridine nucleotide transhydrogenase is important for reaction efficiency. The Journal of Biological Chemistry, 298(9), Article 102304.
Tong, Y., Loonstra, M. R., & Fraaije, M. (2022). Broadening the scope of the Flavin-tag method by improving flavin incorporation and incorporating flavin analogs. ChemBioChem, 23(11), Article e202200144.
Venturi, S., Trajkovic, M., Colombo, D., Brenna, E., Fraaije, M. W., Gatti, F. G., Macchi, P., & Zamboni, E. (2022). Chemoenzymatic Synthesis of the Most Pleasant Stereoisomer of Jessemal. The Journal of Organic Chemistry, 87(9), 6499-6503.
de Gonzalo, G., Lončar, N., & Fraaije, M. (2022). Kinetic resolution of racemic benzofused alcohols catalysed by HMFO variants in presence of natural deep eutectic solvents. Biocatalysis and Biotransformation, 41(2), 145-152.
Guo, Y., Alvigini, L., Trajkovic, M., Alonso-Cotchico, L., Monza, E., Savino, S., Marić, I., Mattevi, A., & Fraaije, M. W. (2022). Structure- and computational-aided engineering of an oxidase to produce isoeugenol from a lignin-derived compound. Nature Communications, 13(1), Article 7195.


Gran-Scheuch, A., Parra, L., & Fraaije, M. W. (2023). Systematic Assessment of Uncoupling in Flavoprotein Oxidases and Monooxygenases. ACS Sustainable Chemistry and Engineering, 4948–4959.
Rembeza, E., Boverio, A., Fraaije, M. W., & Engqvist, M. (2022). Discovery of two novel oxidases using a high-throughput activity screen. ChemBioChem, 23(2), Article e202100510.
Lee, M., Drenth, J., Trajkovic, M., de Jong, R. M., & Fraaije, M. W. (2022). Introducing an Artificial Deazaflavin Cofactor in Escherichia coli and Saccharomyces cerevisiae. ACS Synthetic Biology, 11(2), 938-952.
Drenth, J., Yang, G., Paul, C. E., & Fraaije, M. W. (2021). A Tailor-Made Deazaflavin-Mediated Recycling System for Artificial Nicotinamide Cofactor Biomimetics. ACS Catalysis, 11, 11561-11569.
Laura, A., Gran-Scheuch, A., Guo, Y., Trajkovic, M., Saifuddin, M., Fraaije, M. W., & Mattevi, A. (2021). Discovery, Biocatalytic Exploration and Structural Analysis of a 4-Ethylphenol Oxidase from Gulosibacter chungangensis. ChemBioChem, 22(22), 3225-3233. Article cbic.202100457.
Tjallinks, G., Martin, C., & Fraaije, M. W. (2021). Enantioselective oxidation of secondary alcohols by the flavoprotein alcohol oxidase from Phanerochaete chrysosporium. Archives of Biochemistry and Biophysics, 704, Article 108888.
Cibulka, R., & Fraaije, M. W. (2021). Flavin-Based Catalysis - Preface. In R. Cibulka, & M. Fraaije (Eds.), Flavin-Based Catalysis: Principles and Applications (pp. xi-xii). Wiley.
Tong, Y., Lee, M., Drenth, J., & Fraaije, M. W. (2021). Flavin-Tag: A Facile Method for Site-Specific Labeling of Proteins with a Flavin Fluorophore. BIOCONJUGATE CHEMISTRY, 32(8), 1559-1563.
Savino, S., Daniël‐Moráh Meijer, J., Rozeboom, H. J., van Beek, H. L., & Fraaije, M. W. (2021). Kinetic and structural properties of a robust bacterial L-amino acid oxidase. Catalysts, 11(11), Article 1309.
Fraaije, M. W., Trajkovic, M., & Martin, C. (2021). Means and methods for selective double diol oxidation. (Patent No. WO2021071356).
Cibulka, R., & Fraaije, M. W. (2021). Modes of Flavin-Based Catalysis. In R. Cibulka, & M. W. Fraaije (Eds.), Flavin-Based Catalysis: Principles and Applications (pp. 97-124). Wiley.
Purwani, N. N., Martin, C., Savino, S., & Fraaije, M. W. (2021). Modular assembly of phosphite dehydrogenase and phenylacetone monooxygenase for tuning cofactor regeneration. Biomolecules, 11(6), Article 905.
Drenth, J., & Fraaije, M. W. (2021). Natural Flavins: Occurrence, Role, and Noncanonical Chemistry. In R. Cibulka, & M. W. F. (Eds.), Flavin-Based Catalysis: Principles and Applications (pp. 29-65). Wiley.
Mascotti, M. L., Juri Ayub, M., & Fraaije, M. W. (2021). On the diversity of F420 -dependent oxidoreductases: A sequence- and structure-based classification. Proteins, 89(11), 1497-1507. Article 26170.
Gran-Scheuch, A., Aalbers, F., Woudstra, Y., Parra, L., & Fraaije, M. W. (2021). Optimizing the linker length for fusing an alcohol dehydrogenase with a cyclohexanone monooxygenase. In M. Merkx (Ed.), Linkers in Biomacromolecules (Vol. 647, pp. 107-143). (Methods in Enzymology). Elsevier.
Lončar, N., Konukoglu, F., Fraaije, M., Eryilmaz, J., & Tuncer, E. (2021). Process for dyeing textiles. (Patent No. WO2021013371).
Zuccarello, L., Barbosa, C., Galdino, E., Lončar, N., Silveira, C. M., Fraaije, M. W., & Todorovic, S. (2021). Serr spectroelectrochemistry as a guide for rational design of dyp-based bioelectronics devices. International Journal of Molecular Sciences, 22(15), Article 7998.
Kardashliev, T., Weingartner, A., Romero, E., Schwaneberg, U., Fraaije, M., Panke, S., & Held, M. (2021). Whole-cell screening of oxidative enzymes using genetically encoded sensors. Chemical Science, 12(44), 14766-14772. Article d1sc02578c.


Bailleul, G., Nicoll, C. R., Mascotti, M. L., Mattevi, A., & Fraaije, M. W. (2021). Ancestral reconstruction of mammalian FMO1 enables structural determination, revealing unique features that explain its catalytic properties. The Journal of Biological Chemistry, 296, Article 100221.
Martin, C., Tjallinks, G., Trajkovic, M., & Fraaije, M. (2021). Facile Stereoselective Reduction of Prochiral Ketones by using an F 420-dependent alcohol dehydrogenase. ChemBioChem, 22(1), 156-159. Article cbic.202000651.
Zitare, U. A., Habib, M. H., Rozeboom, H., Mascotti, M. L., Todorovic, S., & Fraaije, M. W. (2021). Mutational and structural analysis of an ancestral fungal dye decolorizing peroxidase. The FEBS Journal, 288(11), 3602-3618. Article febs.15687.
Savino, S., Jensen, S., Terwisscha van Scheltinga, A., & Fraaije, M. W. (2020). Analysis of the structure and substrate scope of chitooligosaccharide oxidase reveals high affinity for C2-modified glucosamines. FEBS Letters, 594(17), 2819-2828.
Aalbers, F. S., Fürst, M. J., Rovida, S., Trajkovic, M., Gómez Castellanos, J. R., Bartsch, S., Vogel, A., Mattevi, A., & Fraaije, M. W. (2020). Approaching boiling point stability of an alcohol dehydrogenase through computationally-guided enzyme engineering. eLife, 9, Article e54639.
Ueoka, R., Meoded, R. A., Gran-Scheuch, A., Bhushan, A., Fraaije, M. W., & Piel, J. (2020). Corrigendum to: Genome Mining of Oxidation Modules in trans -Acyltransferase Polyketide Synthases Reveals a Culturable Source for Lobatamides (Angewandte Chemie International Edition, (2020), 59, 20, (7761-7765), 10.1002/anie.201916005). Angewandte Chemie-International Edition, 59(29), 11698-11698.
Ueoka, R., Meoded, R., Gran-Scheuch, A., Bhushan, A., Fraaije, M., & Piel, J. (2020). Genome mining of oxidation modules in trans-acyltransferase polyketide synthases reveals a culturable source for lobatamides. Angewandte Chemie (International ed. in English), 59(20), 7761-7765.
Román, R., Lončar, N., Casablancas, A., Fraaije, M. W., & Gonzalez, G. (2020). High-level production of industrially relevant oxidases by a two-stage fed-batch approach: Overcoming catabolite repression in arabinose-inducible Escherichia coli systems. Applied Microbiology and Biotechnology, 104(12), 5337-5345.
Romero, E., Savino, S., Fraaije, M. W., & Loncar, N. (2020). Mechanistic and Crystallographic Studies of Azoreductase AzoA from Bacillus wakoensis A01. ACS chemical biology, 15(2), 504-512. Article acschembio.9b00970.
Venturi, S., Brenna, E., Colombo, D., Fraaije, M. W., Gatti, F. G., Macchi, P., Monti, D., Trajkovic, M., & Zamboni, E. (2020). Multienzymatic Stereoselective Reduction of Tetrasubstituted Cyclic Enones to Halohydrins with Three Contiguous Stereogenic Centers. ACS Catalysis, 10(21), 13050-13057.
de Gonzalo, G., Martin, C., & Fraaije, M. W. (2020). Positive Impact of Natural Deep Eutectic Solvents on the Biocatalytic Performance of 5-Hydroxymethyl-Furfural Oxidase. Catalysts, 10(4), Article 447.
Lončar, N., Eryilmaz, J., Senel, E., Kaplan, G., Fraaije, M., & Cobanoglu, O. (2020). Process and apparatus for dyeing textiles. (Patent No. WO2020015839).
Martin, C., Trajkovic, M., & Fraaije, M. (2020). Production of Hydroxy Acids: Selective Double Oxidation of Diols by Flavoprotein Alcohol Oxidase. Angewandte Chemie (International ed. in English), 59(12), 4869-4872.
Fabara, A. N., & Fraaije, M. W. (2020). Production of indigo through the use of a dual-function substrate and a bifunctional fusion enzyme. Enzyme and Microbial Technology, 142, Article 109692.
Nicoll, C. R., Bailleul, G., Fiorentini, F., Mascotti, M. L., Fraaije, M. W., & Mattevi, A. (2020). Publisher Correction: Ancestral-sequence reconstruction unveils the structural basis of function in mammalian FMOs (Nature Structural & Molecular Biology, (2020), 27, 1, (14-24), 10.1038/s41594-019-0347-2). Nature Structural & Molecular Biology, 27(2), 222.
Silveira, C. M., Moe, E., Fraaije, M., Martins, L. O., & Todorovic, S. (2020). Resonance Raman view of the active site architecture in bacterial DyP-type peroxidases. RSC Advances, 10(19), 11095-11104.
Lončar, N., Rozeboom, H. J., Franken, L. E., Stuart, M. C. A., & Fraaije, M. W. (2020). Structure of a robust bacterial protein cage and its application as a versatile biocatalytic platform through enzyme encapsulation. Biochemical and Biophysical Research Communications, 529(3), 548-553. Article j.bbrc.2020.06.059.
Tong, Y., Trajkovic, M., Savino, S., van Berkel, W. J. H., & Fraaije, M. W. (2020). Substrate binding tunes the reactivity of hispidin 3-hydroxylase, a flavoprotein monooxygenase involved in fungal bioluminescence. The Journal of Biological Chemistry, 295(47), 16013-16022.
Martin, C., Binda, C., Fraaije, M. W., & Mattevi, A. (2020). The multipurpose family of flavoprotein oxidases. In P. Chaiyen, & F. Tamanoi (Eds.), Flavin-Dependent Enzymes : Mechanisms, Structures and Applications (Vol. 47, pp. 63-86). (The Enzymes). Elsevier.
Savino, S., & Fraaije, M. W. (2020). The vast repertoire of carbohydrate oxidases: An overview. Biotechnology Advances, 51, Article 107634.
Niero, M., Righetto, I., Beneventi, E., de Laureto, P. P., Fraaije, M. W., Filippini, F., & Bergantino, E. (2020). Unique Features of a New Baeyer-Villiger Monooxygenase from a Halophilic Archaeon. Catalysts, 10(1), Article 128.
Ewing, T. A., Gygli, G., Fraaije, M. W., & van Berkel, W. J. H. (2020). Vanillyl alcohol oxidase. In P. Chaiyen, & F. Tamanoi (Eds.), Flavin-Dependent Enzymes : Mechanisms, Structures and Applications (Vol. 47, pp. 87-116). (The Enzymes). Elsevier.


Fabara, A. N., & Fraaije, M. W. (2020). An overview of microbial indigo-forming enzymes. Applied Microbiology and Biotechnology, 104(3), 925-933.
Maenpuen, S., Pongsupasa, V., Pensook, W., Anuwan, P., Kraivisitkul, N., Pinthong, C., Phonbuppha, J., Luanloet, T., Wijma, H. J., Fraaije, M. W., Lawan, N., Chaiyen, P., Wongnate, T., & Kraivisitkul, N. (2020). Creating Flavin Reductase Variants with Thermostable and Solvent‐Tolerant Properties by Rational‐Design Engineering. ChemBioChem, 21(10), 1481-1491.
Nicoll, C. R., Bailleul, G., Fiorentini, F., Mascotti, M. L., Fraaije, M. W., & Mattevi, A. (2019). Ancestral-sequence reconstruction unveils the structural basis of function in mammalian FMOs. Nature Structural & Molecular Biology, 27(1), 14-24.
Furst, M. J. L. J., Gran-Scheuch, A., Aalbers, F. S., & Fraaije, M. W. (2019). Baeyer-Villiger Monooxygenases: Tunable Oxidative Biocatalysts. ACS Catalysis, 9(12), 11207-11241.
Fürst, M. J. L. J., Fiorentini, F., & Fraaije, M. W. (2019). Beyond active site residues: overall structural dynamics control catalysis in flavin-containing and heme-containing monooxygenases. Current Opinion in Structural Biology, 59, 29-37.
Gandomkar, S., Jost, E., Loidolt, D., Swoboda, A., Pickl, M., Elaily, W., Daniel, B., Fraaije, M. W., Macheroux, P., & Kroutil, W. (2019). Biocatalytic Enantioselective Oxidation of Sec-Allylic Alcohols with Flavin-Dependent Oxidases. Advanced Synthesis & Catalysis, 361(22), 5264-5271.
Habib, M. H., Rozeboom, H. J., & Fraaije, M. W. (2019). Characterization of a New DyP-Peroxidase from the Alkaliphilic Cellulomonad, Cellulomonas bogoriensis. Molecules, 24(7), Article 1208.
Lončar, N., Fiorentini, F., Bailleul, G., Savino, S., Romero, E., Mattevi, A., & Fraaije, M. W. (2019). Characterization of a thermostable flavin-containing monooxygenase from Nitrincola lacisaponensis (NiFMO). Applied Microbiology and Biotechnology, 103(4), 1755-1764.
Drenth, J., Trajkovic, M., & Fraaije, M. W. (2019). Chemoenzymatic Synthesis of an Unnatural Deazaflavin Cofactor That Can Fuel F-420-Dependent Enzymes. ACS Catalysis, 9(7), 6435-6443.
Huang, L., Aalbers, F. S., Tang, W., Röllig, R., Fraaije, M. W., & Kara, S. (2019). Convergent cascade catalyzed by monooxygenase - alcohol dehydrogenase fusion applied in organic media. ChemBioChem, 20(13), 1653-1658.
Tischler, D., van Berkel, W. J. H., & Fraaije, M. W. (2019). Editorial: Actinobacteria, a Source of Biocatalytic Tools. Frontiers in Microbiology, 10, Article 800.
Fürst, M., Kerschbaumer, B., Rinnofner, C., Migglautsch, A., Winkler, M., & Fraaije, M. (2019). Exploring the Biocatalytic Potential of a Self-Sufficient Cytochr ome P450 from Thermothelomyces thermophila. Advanced Synthesis & Catalysis, 361(11), 2487–2496.
Loncar, N., Draskovic, N., Bozic, N., Romero, E., Simic, S., Opsenica, I., Vujcic, Z., & Fraaije, M. W. (2019). Expression and Characterization of a Dye-Decolorizing Peroxidase from Pseudomonas Fluorescens Pf0-1. Catalysts, 9(5), Article 463.
Fraaije, M. W., Romero Guzman, E., Mattevi, A., & Nguyen, Q.-T. (2019). Mutant alcohol oxidases and use thereof in the conversion of diols and polyols. (Patent No. WO2019212351).
Fürst, M., Boonstra, M., Bandstra, S., & Fraaije, M. (2019). Stabilization of cyclohexanone monooxygenase by computational and experimental library design. Biotechnology and Bioengineering, 116(9), 2167-2177.
Lončar, N., van Beek, H. L., & Fraaije, M. W. (2019). Structure-Based Redesign of a Self-Sufficient Flavin-Containing Monooxygenase towards Indigo Production. International Journal of Molecular Sciences, 20(24), Article 6148.
Mourelle-Insua, A., Aalbers, F. S., Lavandera, I., Gotor-Fernandez, V., & Fraaije, M. W. (2019). What to sacrifice? Fusions of cofactor regenerating enzymes with Baeyer-Villiger monooxygenases and alcohol dehydrogenases for self-sufficient redox biocatalysis. Tetrahedron, 75(13), 1832-1839.


Aalbers, F., & Fraaije, M. (2019). Design of artificial alcohol oxidases: alcohol dehydrogenase - NADPH oxidase fusions for continuous oxidations. ChemBioChem, 20(1), 51-56. Article cbic.201800421.
Aalbers, F., & Fraaije, M. (2019). Enzyme fusions in biocatalysis: Coupling reactions by pairing enzymes. ChemBioChem, 20(1), 20-28. Article cbic.201800394.
Ferrari, A. R., Rozeboom, H. J., Vugts, A. S. C., Koetsier, M. J., Floor, R., & Fraaije, M. W. (2018). Characterization of Two VAO-Type Flavoprotein Oxidases from Myceliophthora thermophila. Molecules, 23(1).
Martin, C., Ovalle Maqueo, A., Wijma, H. J., & Fraaije, M. W. (2018). Creating a more robust 5-hydroxymethylfurfural oxidase by combining computational predictions with a novel effective library design. Biotechnology for Biofuels, 11, 1-9. Article 56.
Mathew, S., Trajkovic, M., Kumar, H., Nguyen, Q.-T., & Fraaije, M. W. (2018). Enantio- and regioselective ene-reductions using F420H2-dependent enzymes. Chemical communications (Cambridge, England), 54(79), 11208-11211. Article C8CC04449J.
Fürst, M., Martin, C., Lončar, N., & Fraaije, M. (2018). Experimental Protocols for Generating Focused Mutant Libraries and Screening for Thermostable Proteins. In N. Scrutton (Ed.), Enzymes in Synthetic Biology (pp. 151-187). (Methods in Enzymology; Vol. 608). Academic Press.
Pickl, M., Swoboda, A., Romero, E., Winkler, C., Binda, C., Mattevi, A., Faber, K., & Fraaije, M. (2018). Kinetic Resolution of sec-Thiols by Enantioselective Oxidation with Rationally Engineered 5-(Hydroxymethyl) furfural Oxidase. Angewandte Chemie - International Edition, 57(11), 2864-2868. Article anie.201713189.
Gran-Scheuch, A., Trajkovic, M., Parra, L., & Fraaije, M. W. (2018). Mining the Genome of: Two New Type I Baeyer-Villiger Monooxygenases From Atacama Desert. Frontiers in Microbiology, 9, Article 1609.
Li, G., Garcia-Borràs, M., Fürst, M., Ilie, A., Fraaije, M., Houk, K. N., & Reetz, M. T. (2018). Overriding Traditional Electronic Effects in Biocatalytic Baeyer-Villiger Reactions by Directed Evolution. Journal of the American Chemical Society, 140(33), 10464-10472. Article jacs.8b04742.
Mascotti, M. L., Kumar, H., Nguyen, Q.-T., Ayub, M. J., & Fraaije, M. W. (2018). Reconstructing the evolutionary history of F-420-dependent dehydrogenases. Scientific Reports, 8(1), Article 17571.
Romero, E., Gómez Castellanos, J. R., Gadda, G., Fraaije, M. W., & Mattevi, A. (2018). Same Substrate, Many Reactions: Oxygen Activation in Flavoenzymes. Chemical reviews, 118(4), 1742-1769.


Beyer, N., Kulig, J. K., Fraaije, M. W., Hayes, M. A., & Janssen, D. B. (2018). Exploring PTDH-P450BM3 variants for the synthesis of drug metabolites. ChemBioChem, 19(4), 326-337. Article cbic.201700470.
Delgove, M., Fürst, M., Fraaije, M., Bernaerts, K., & De Wildeman, S. M. A. (2018). Exploring the substrate scope of Baeyer-Villiger monooxygenases with branched lactones as entry towards polyesters. ChemBioChem, 19(4), 354-360.
Burgener, S., Schwander, T., Romero, E., Fraaije, M. W., & Erb, T. J. (2018). Molecular Basis for Converting (2S)-Methylsuccinyl-CoA Dehydrogenase into an Oxidase. Molecules, 23(1), Article 68.
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