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Fraaije, Prof. Marco

Marco Fraaije

Prof. Marco W. Fraaije studied Molecular Science at Wageningen University & Research, where he also gained his PhD. He was then a postdoctoral researcher at the Department of Genetics and Microbiology at the University of Pavia (Italy) and has worked at the University of Groningen since 1999. When he was an assistant professor, he led several studies centred around discovering new biocatalysts (enzymes) and researching their function.

At present, he is Professor of Molecular Enzymology. In 2016, he was awarded a Vici research grant and in 2018, he received the BIOCAT Science Award for his academic contribution to the biotechnology research field. Fraaije has published hundreds of articles, with a main focus on biocatalysis and enzyme engineering. His research revolves around discovering and designing new enzymes, focusing particularly on flavine adenine dinucleotide enzymes: those that use vitamin B2 to function.

In addition to the academic applications of his research, Fraaije is also building a bridge between the worlds of academia and industry. For example, he is the co-founder and current advisor of start-up company Gecco Biotech. Additionally, he has coordinated several EU-funded initiatives, among which the ROBOX project. This project is mainly aimed at designing robust enzymes that can be used in oxidation processes. These enzymes can replace chemical reactions that are currently being used in production processes. The aim is to reduce the need to use precious metals and toxic substances. Furthermore, his research group is working on computer-based engineering methods to adjust enzymes quickly for biotechnological applications.


Partipilo, M., Yang, G., Mascotti, L., Slotboom, D., & Fraaije, M. (2022). A conserved sequence motif in the E. coli soluble FAD-containing pyridine nucleotide transhydrogenase is important for reaction efficiency. The Journal of Biological Chemistry, 298(9), [102304].
Tong, Y., Loonstra, M. R., & Fraaije, M. (2022). Broadening the scope of the Flavin-tag method by improving flavin incorporation and incorporating flavin analogs. ChemBioChem, 23(11), [e202200144].
Venturi, S., Trajkovic, M., Colombo, D., Brenna, E., Fraaije, M. W., Gatti, F. G., Macchi, P., & Zamboni, E. (2022). Chemoenzymatic Synthesis of the Most Pleasant Stereoisomer of Jessemal. The Journal of Organic Chemistry, 87(9), 6499-6503.
de Gonzalo, G., Lončar, N., & Fraaije, M. (2022). Kinetic resolution of racemic benzofused alcohols catalysed by HMFO variants in presence of natural deep eutectic solvents. Biocatalysis and Biotransformation.
Boverio, A., Widodo, W. S., Santema, L. L., Rozeboom, H. J., Xiang, R., Guallar, V., Mattevi, A., & Fraaije, M. W. (2022). Structural Elucidation and Engineering of a Bacterial Carbohydrate Oxidase. Biochemistry, [2c00307].
de Gonzalo, G., Loncar, N., & Fraaije, M. (2022). Sulphoxidation reactions catalysed by the Baeyer-Villiger monooxygenase OTEMO from Pseudomonas putida ATCC 17453. Biocatalysis and Biotransformation, [2113519].


Rembeza, E., Boverio, A., Fraaije, M. W., & Engqvist, M. (2022). Discovery of two novel oxidases using a high-throughput activity screen. ChemBioChem, 23(2), [e202100510].
Lee, M., Drenth, J., Trajkovic, M., de Jong, R. M., & Fraaije, M. W. (2022). Introducing an Artificial Deazaflavin Cofactor in Escherichia coli and Saccharomyces cerevisiae. ACS Synthetic Biology, 11(2), 938-952.
Drenth, J., Yang, G., Paul, C. E., & Fraaije, M. W. (2021). A Tailor-Made Deazaflavin-Mediated Recycling System for Artificial Nicotinamide Cofactor Biomimetics. ACS Catalysis, 11, 11561-11569.
Laura, A., Gran-Scheuch, A., Guo, Y., Trajkovic, M., Saifuddin, M., Fraaije, M. W., & Mattevi, A. (2021). Discovery, biocatalytic exploration and structural analysis of a 4-ethylphenol oxidase from Gulosibacter chungangensis. ChemBioChem, [cbic.202100457].
Tjallinks, G., Martin, C., & Fraaije, M. W. (2021). Enantioselective oxidation of secondary alcohols by the flavoprotein alcohol oxidase from Phanerochaete chrysosporium. Archives of Biochemistry and Biophysics, 704, [108888].
Tong, Y., Lee, M., Drenth, J., & Fraaije, M. W. (2021). Flavin-Tag: A Facile Method for Site-Specific Labeling of Proteins with a Flavin Fluorophore. BIOCONJUGATE CHEMISTRY, 32(8), 1559-1563.
Savino, S., Daniël‐Moráh Meijer, J., Rozeboom, H. J., van Beek, H. L., & Fraaije, M. W. (2021). Kinetic and structural properties of a robust bacterial L-amino acid oxidase. Catalysts, 11(11), [1309].
Fraaije, M. W., Trajkovic, M., & Martin, C. (2021). Means and methods for selective double diol oxidation. (Patent No. WO2021071356).
Purwani, N. N., Martin, C., Savino, S., & Fraaije, M. W. (2021). Modular assembly of phosphite dehydrogenase and phenylacetone monooxygenase for tuning cofactor regeneration. Biomolecules, 11(6), [905].
Mascotti, M. L., Juri Ayub, M., & Fraaije, M. W. (2021). On the diversity of F420 -dependent oxidoreductases: A sequence- and structure-based classification. Proteins, 89(11), 1497-1507. [26170].
Gran-Scheuch, A., Aalbers, F., Woudstra, Y., Parra, L., & Fraaije, M. W. (2021). Optimizing the linker length for fusing an alcohol dehydrogenase with a cyclohexanone monooxygenase. In M. Merkx (Ed.), Linkers in Biomacromolecules (Vol. 647, pp. 107-143). (Methods in Enzymology). Elsevier.
Lončar, N., Konukoglu, F., Fraaije, M., Eryilmaz, J., & Tuncer, E. (2021). Process for dyeing textiles. (Patent No. WO2021013371).
Zuccarello, L., Barbosa, C., Galdino, E., Lončar, N., Silveira, C. M., Fraaije, M. W., & Todorovic, S. (2021). Serr spectroelectrochemistry as a guide for rational design of dyp-based bioelectronics devices. International Journal of Molecular Sciences, 22(15), [7998].
Gran-Scheuch, A., Parra, L., & Fraaije, M. W. (2021). Systematic Assessment of Uncoupling in Flavoprotein Oxidases and Monooxygenases. ACS Sustainable Chemistry and Engineering.
Kardashliev, T., Weingartner, A., Romero, E., Schwaneberg, U., Fraaije, M., Panke, S., & Held, M. (2021). Whole-cell screening of oxidative enzymes using genetically encoded sensors. Chemical Science, 12(44), 14766-14772. [d1sc02578c].


Bailleul, G., Nicoll, C. R., Mascotti, M. L., Mattevi, A., & Fraaije, M. W. (2021). Ancestral reconstruction of mammalian FMO1 enables structural determination, revealing unique features that explain its catalytic properties. The Journal of Biological Chemistry, 296, [100221].
Martin, C., Tjallinks, G., Trajkovic, M., & Fraaije, M. (2021). Facile Stereoselective Reduction of Prochiral Ketones by using an F 420-dependent alcohol dehydrogenase. ChemBioChem, 22(1), 156-159. [cbic.202000651].
Zitare, U. A., Habib, M. H., Rozeboom, H., Mascotti, M. L., Todorovic, S., & Fraaije, M. W. (2021). Mutational and structural analysis of an ancestral fungal dye decolorizing peroxidase. The FEBS Journal, 288(11), 3602-3618. [febs.15687].
Savino, S., Jensen, S., Terwisscha van Scheltinga, A., & Fraaije, M. W. (2020). Analysis of the structure and substrate scope of chitooligosaccharide oxidase reveals high affinity for C2-modified glucosamines. FEBS Letters, 594(17), 2819-2828.
Aalbers, F. S., Fürst, M. J., Rovida, S., Trajkovic, M., Gómez Castellanos, J. R., Bartsch, S., Vogel, A., Mattevi, A., & Fraaije, M. W. (2020). Approaching boiling point stability of an alcohol dehydrogenase through computationally-guided enzyme engineering. eLife, 9, [ e54639].
Ueoka, R., Meoded, R. A., Gran-Scheuch, A., Bhushan, A., Fraaije, M. W., & Piel, J. (2020). Corrigendum to: Genome Mining of Oxidation Modules in trans -Acyltransferase Polyketide Synthases Reveals a Culturable Source for Lobatamides (Angewandte Chemie International Edition, (2020), 59, 20, (7761-7765), 10.1002/anie.201916005). Angewandte Chemie-International Edition, 59(29), 11698-11698.
Ueoka, R., Meoded, R., Gran-Scheuch, A., Bhushan, A., Fraaije, M., & Piel, J. (2020). Genome mining of oxidation modules in trans-acyltransferase polyketide synthases reveals a culturable source for lobatamides. Angewandte Chemie (International ed. in English), 59(20), 7761-7765.
Román, R., Lončar, N., Casablancas, A., Fraaije, M. W., & Gonzalez, G. (2020). High-level production of industrially relevant oxidases by a two-stage fed-batch approach: Overcoming catabolite repression in arabinose-inducible Escherichia coli systems. Applied Microbiology and Biotechnology, 104(12), 5337-5345.
Romero, E., Savino, S., Fraaije, M. W., & Loncar, N. (2020). Mechanistic and Crystallographic Studies of Azoreductase AzoA from Bacillus wakoensis A01. ACS chemical biology, 15(2), 504-512. [acschembio.9b00970].
Venturi, S., Brenna, E., Colombo, D., Fraaije, M. W., Gatti, F. G., Macchi, P., Monti, D., Trajkovic, M., & Zamboni, E. (2020). Multienzymatic Stereoselective Reduction of Tetrasubstituted Cyclic Enones to Halohydrins with Three Contiguous Stereogenic Centers. ACS Catalysis, 10(21), 13050-13057.
de Gonzalo, G., Martin, C., & Fraaije, M. W. (2020). Positive Impact of Natural Deep Eutectic Solvents on the Biocatalytic Performance of 5-Hydroxymethyl-Furfural Oxidase. Catalysts, 10(4), [447].
Lončar, N., Eryilmaz, J., Senel, E., Kaplan, G., Fraaije, M., & Cobanoglu, O. (2020). Process and apparatus for dyeing textiles. (Patent No. WO2020015839).
Martin, C., Trajkovic, M., & Fraaije, M. (2020). Production of Hydroxy Acids: Selective Double Oxidation of Diols by Flavoprotein Alcohol Oxidase. Angewandte Chemie (International ed. in English), 59(12), 4869-4872.
Fabara, A. N., & Fraaije, M. W. (2020). Production of indigo through the use of a dual-function substrate and a bifunctional fusion enzyme. Enzyme and Microbial Technology, 142, [109692].
Nicoll, C. R., Bailleul, G., Fiorentini, F., Mascotti, M. L., Fraaije, M. W., & Mattevi, A. (2020). Publisher Correction: Ancestral-sequence reconstruction unveils the structural basis of function in mammalian FMOs (Nature Structural & Molecular Biology, (2020), 27, 1, (14-24), 10.1038/s41594-019-0347-2). Nature Structural & Molecular Biology, 27(2), 222.
Silveira, C. M., Moe, E., Fraaije, M., Martins, L. O., & Todorovic, S. (2020). Resonance Raman view of the active site architecture in bacterial DyP-type peroxidases. RSC Advances, 10(19), 11095-11104.
Lončar, N., Rozeboom, H. J., Franken, L. E., Stuart, M. C. A., & Fraaije, M. W. (2020). Structure of a robust bacterial protein cage and its application as a versatile biocatalytic platform through enzyme encapsulation. Biochemical and Biophysical Research Communications, 529(3), 548-553. [j.bbrc.2020.06.059].
Tong, Y., Trajkovic, M., Savino, S., van Berkel, W. J. H., & Fraaije, M. W. (2020). Substrate binding tunes the reactivity of hispidin 3-hydroxylase, a flavoprotein monooxygenase involved in fungal bioluminescence. The Journal of Biological Chemistry, 295(47), 16013-16022.
Martin, C., Binda, C., Fraaije, M. W., & Mattevi, A. (2020). The multipurpose family of flavoprotein oxidases. In P. Chaiyen, & F. Tamanoi (Eds.), Flavin-Dependent Enzymes : Mechanisms, Structures and Applications (Vol. 47, pp. 63-86). (The Enzymes). Elsevier.
Savino, S., & Fraaije, M. W. (2020). The vast repertoire of carbohydrate oxidases: An overview. Biotechnology Advances, 51, [107634].
Niero, M., Righetto, I., Beneventi, E., de Laureto, P. P., Fraaije, M. W., Filippini, F., & Bergantino, E. (2020). Unique Features of a New Baeyer-Villiger Monooxygenase from a Halophilic Archaeon. Catalysts, 10(1), [128].
Ewing, T. A., Gygli, G., Fraaije, M. W., & van Berkel, W. J. H. (2020). Vanillyl alcohol oxidase. In P. Chaiyen, & F. Tamanoi (Eds.), Flavin-Dependent Enzymes : Mechanisms, Structures and Applications (Vol. 47, pp. 87-116). (The Enzymes). Elsevier.


Fabara, A. N., & Fraaije, M. W. (2020). An overview of microbial indigo-forming enzymes. Applied Microbiology and Biotechnology, 104(3), 925-933.
Maenpuen, S., Pongsupasa, V., Pensook, W., Anuwan, P., Kraivisitkul, N., Pinthong, C., Phonbuppha, J., Luanloet, T., Wijma, H. J., Fraaije, M. W., Lawan, N., Chaiyen, P., Wongnate, T., & Kraivisitkul, N. (2020). Creating Flavin Reductase Variants with Thermostable and Solvent‐Tolerant Properties by Rational‐Design Engineering. ChemBioChem, 21(10), 1481-1491.
Nicoll, C. R., Bailleul, G., Fiorentini, F., Mascotti, M. L., Fraaije, M. W., & Mattevi, A. (2019). Ancestral-sequence reconstruction unveils the structural basis of function in mammalian FMOs. Nature Structural & Molecular Biology, 27(1), 14-24.
Furst, M. J. L. J., Gran-Scheuch, A., Aalbers, F. S., & Fraaije, M. W. (2019). Baeyer-Villiger Monooxygenases: Tunable Oxidative Biocatalysts. ACS Catalysis, 9(12), 11207-11241.
Fürst, M. J. L. J., Fiorentini, F., & Fraaije, M. W. (2019). Beyond active site residues: overall structural dynamics control catalysis in flavin-containing and heme-containing monooxygenases. Current Opinion in Structural Biology, 59, 29-37.
Gandomkar, S., Jost, E., Loidolt, D., Swoboda, A., Pickl, M., Elaily, W., Daniel, B., Fraaije, M. W., Macheroux, P., & Kroutil, W. (2019). Biocatalytic Enantioselective Oxidation of Sec-Allylic Alcohols with Flavin-Dependent Oxidases. Advanced Synthesis & Catalysis, 361(22), 5264-5271.
Habib, M. H., Rozeboom, H. J., & Fraaije, M. W. (2019). Characterization of a New DyP-Peroxidase from the Alkaliphilic Cellulomonad, Cellulomonas bogoriensis. Molecules, 24(7), [1208].
Lončar, N., Fiorentini, F., Bailleul, G., Savino, S., Romero, E., Mattevi, A., & Fraaije, M. W. (2019). Characterization of a thermostable flavin-containing monooxygenase from Nitrincola lacisaponensis (NiFMO). Applied Microbiology and Biotechnology, 103(4), 1755-1764.
Drenth, J., Trajkovic, M., & Fraaije, M. W. (2019). Chemoenzymatic Synthesis of an Unnatural Deazaflavin Cofactor That Can Fuel F-420-Dependent Enzymes. ACS Catalysis, 9(7), 6435-6443.
Huang, L., Aalbers, F. S., Tang, W., Röllig, R., Fraaije, M. W., & Kara, S. (2019). Convergent cascade catalyzed by monooxygenase - alcohol dehydrogenase fusion applied in organic media. ChemBioChem, 20(13), 1653-1658.
Tischler, D., van Berkel, W. J. H., & Fraaije, M. W. (2019). Editorial: Actinobacteria, a Source of Biocatalytic Tools. Frontiers in Microbiology, 10, [800].
Fürst, M., Kerschbaumer, B., Rinnofner, C., Migglautsch, A., Winkler, M., & Fraaije, M. (2019). Exploring the Biocatalytic Potential of a Self-Sufficient Cytochr ome P450 from Thermothelomyces thermophila. Advanced Synthesis & Catalysis, 361(11), 2487–2496.
Loncar, N., Draskovic, N., Bozic, N., Romero, E., Simic, S., Opsenica, I., Vujcic, Z., & Fraaije, M. W. (2019). Expression and Characterization of a Dye-Decolorizing Peroxidase from Pseudomonas Fluorescens Pf0-1. Catalysts, 9(5), [463].
Fraaije, M. W., Romero Guzman, E., Mattevi, A., & Nguyen, Q-T. (2019). Mutant alcohol oxidases and use thereof in the conversion of diols and polyols. (Patent No. WO2019212351).
Fürst, M., Boonstra, M., Bandstra, S., & Fraaije, M. (2019). Stabilization of cyclohexanone monooxygenase by computational and experimental library design. Biotechnology and Bioengineering, 116(9), 2167-2177.
Lončar, N., van Beek, H. L., & Fraaije, M. W. (2019). Structure-Based Redesign of a Self-Sufficient Flavin-Containing Monooxygenase towards Indigo Production. International Journal of Molecular Sciences, 20(24), [6148].
Mourelle-Insua, A., Aalbers, F. S., Lavandera, I., Gotor-Fernandez, V., & Fraaije, M. W. (2019). What to sacrifice? Fusions of cofactor regenerating enzymes with Baeyer-Villiger monooxygenases and alcohol dehydrogenases for self-sufficient redox biocatalysis. Tetrahedron, 75(13), 1832-1839.


Aalbers, F., & Fraaije, M. (2019). Design of artificial alcohol oxidases: alcohol dehydrogenase - NADPH oxidase fusions for continuous oxidations. ChemBioChem, 20(1), 51-56. [cbic.201800421].
Aalbers, F., & Fraaije, M. (2019). Enzyme fusions in biocatalysis: Coupling reactions by pairing enzymes. ChemBioChem, 20(1), 20-28. [cbic.201800394].
Ferrari, A. R., Rozeboom, H. J., Vugts, A. S. C., Koetsier, M. J., Floor, R., & Fraaije, M. W. (2018). Characterization of Two VAO-Type Flavoprotein Oxidases from Myceliophthora thermophila. Molecules, 23(1).
Martin, C., Ovalle Maqueo, A., Wijma, H. J., & Fraaije, M. W. (2018). Creating a more robust 5-hydroxymethylfurfural oxidase by combining computational predictions with a novel effective library design. Biotechnology for Biofuels, 11, 1-9. [56].
Mathew, S., Trajkovic, M., Kumar, H., Nguyen, Q-T., & Fraaije, M. W. (2018). Enantio- and regioselective ene-reductions using F420H2-dependent enzymes. Chemical communications (Cambridge, England), 54(79), 11208-11211. [C8CC04449J].
Fürst, M., Martin, C., Lončar, N., & Fraaije, M. (2018). Experimental Protocols for Generating Focused Mutant Libraries and Screening for Thermostable Proteins. In N. Scrutton (Ed.), Enzymes in Synthetic Biology (pp. 151-187). (Methods in Enzymology; Vol. 608). Academic Press.
Pickl, M., Swoboda, A., Romero, E., Winkler, C., Binda, C., Mattevi, A., Faber, K., & Fraaije, M. (2018). Kinetic Resolution of sec-Thiols by Enantioselective Oxidation with Rationally Engineered 5-(Hydroxymethyl) furfural Oxidase. Angewandte Chemie - International Edition, 57(11), 2864-2868. [anie.201713189].
Gran-Scheuch, A., Trajkovic, M., Parra, L., & Fraaije, M. W. (2018). Mining the Genome of: Two New Type I Baeyer-Villiger Monooxygenases From Atacama Desert. Frontiers in Microbiology, 9, [1609].
Li, G., Garcia-Borràs, M., Fürst, M., Ilie, A., Fraaije, M., Houk, K. N., & Reetz, M. T. (2018). Overriding Traditional Electronic Effects in Biocatalytic Baeyer-Villiger Reactions by Directed Evolution. Journal of the American Chemical Society, 140(33), 10464-10472. [jacs.8b04742].
Mascotti, M. L., Kumar, H., Nguyen, Q-T., Ayub, M. J., & Fraaije, M. W. (2018). Reconstructing the evolutionary history of F-420-dependent dehydrogenases. Scientific Reports, 8(1), [17571].
Romero, E., Gómez Castellanos, J. R., Gadda, G., Fraaije, M. W., & Mattevi, A. (2018). Same Substrate, Many Reactions: Oxygen Activation in Flavoenzymes. Chemical reviews, 118(4), 1742-1769.
Fürst, M., Romero Guzman, E., Gómez Castellanos, J. R., Fraaije, M., & Mattevi, A. (2018). Side-Chain Pruning Has Limited Impact on Substrate Preference in a Promiscuous Enzyme. ACS Catalysis, 8(12), 11648-11656.
Nguyen, Q-T., Romero, E., Dijkman, W., de Vasconcellos, S. P., Binda, C., Mattevi, A., & Fraaije, M. W. (2018). Structure-based engineering of Phanerochaete chrysosporium alcohol oxidase for enhanced oxidative power towards glycerol. Biochemistry, 57(43), 6209-6218.
Habib, M., Trajkovic, M., & Fraaije, M. W. (2018). The Biocatalytic Synthesis of Syringaresinol from 2,6-Dimethoxy-4-allylphenol in One-Pot Using a Tailored Oxidase/Peroxidase System. ACS Catalysis, 8(6), 5549-5552.


Beyer, N., Kulig, J. K., Fraaije, M. W., Hayes, M. A., & Janssen, D. B. (2018). Exploring PTDH-P450BM3 variants for the synthesis of drug metabolites. ChemBioChem, 19(4), 326-337. [cbic.201700470].
Delgove, M., Fürst, M., Fraaije, M., Bernaerts, K., & De Wildeman, S. M. A. (2018). Exploring the substrate scope of Baeyer-Villiger monooxygenases with branched lactones as entry towards polyesters. ChemBioChem, 19(4), 354-360.
Burgener, S., Schwander, T., Romero, E., Fraaije, M. W., & Erb, T. J. (2018). Molecular Basis for Converting (2S)-Methylsuccinyl-CoA Dehydrogenase into an Oxidase. Molecules, 23(1), [68].
Habib, M. H. M., Deuss, P. J., Loncar, N., Trajkovic, M., & Fraaije, M. W. (2017). A Biocatalytic One-Pot Approach for the Preparation of Lignin Oligomers Using an Oxidase/Peroxidase Cascade Enzyme System. Advanced Synthesis & Catalysis, 359(19), 3354-3361.
Fiorentini, F., Romero, E., Fraaije, M. W., Faber, K., Hall, M., & Mattevi, A. (2017). Baeyer-Villiger Monooxygenase FMO5 as Entry Point in Drug Metabolism. ACS chemical biology, 12(9), 2379-2387.
Kumar, H., & Fraaije, M. (2017). Conversion of Furans by Baeyer-Villiger Monooxygenases. Catalysts, 7(6), [179].
Aalbers, F. S., & Fraaije, M. W. (2017). Coupled reactions by coupled enzymes: alcohol to lactone cascade with alcohol dehydrogenase-cyclohexanone monooxygenase fusions. Applied Microbiology and Biotechnology, 20(101), 7557-7565.
Colpa, D. I., Loncar, N., Schmidt, M., & Fraaije, M. (2017). Creating peroxidase-oxidase fusion enzymes as toolbox for cascade reactions. ChemBioChem, 18, 2226-2230. [cbic.201700478].
Magnani, F., Nenci, S., Fananas, E. M., Ceccon, M., Romero, E., Fraaije, M. W., & Mattevi, A. (2017). Crystal structures and atomic model of NADPH oxidase. Proceedings of the National Academy of Science of the United States of America, 114(26), 6764-6769.
van Beek, H. L., Romero, E., & Fraaije, M. W. (2017). Engineering Cyclohexanone Monooxygenase for the Production of Methyl Propanoate. ACS chemical biology, 12(1), 291-299. [acschembio.6b00965].
Nguyen, Q. T., Mattevi, A., & Fraaije, M. W. (2017). Expanding the Repertoire of Flavoenzyme-Based Biocatalysis. In T. Matsuda (Ed.), Future Directions in Biocatalysis: Second Edition (2nd ed., pp. 119-133). Elsevier.
Oberleitner, N., Ressmann, A. K., Bica, K., Gaertner, P., Fraaije, M. W., Bornscheuer, U. T., Rudroff, F., & Mihovilovic, M. D. (2017). From waste to value - direct utilization of limonene from orange peel in a biocatalytic cascade reaction towards chiral carvolactone. Green Chemistry, 19(2), 367-371.
Kumar, H., Nguyen, Q-T., Binda, C., Mattevi, A., & Fraaije, M. W. (2017). Isolation and characterization of a thermostable F420:NADPH oxidoreductase from Thermobifida fusca. The Journal of Biological Chemistry, 292, 10123-10130. [jbc.M117.787754].
Li, G., Fürst, M. J. L. J., Mansouri, H. R., Ressmann, A. K., Ilie, A., Rudroff, F., Mihovilovic, M. D., Fraaije, M. W., & Reetz, M. T. (2017). Manipulating the stereoselectivity of the thermostable Baeyer-Villiger monooxygenase TmCHMO by directed evolution. Organic & Biomolecular Chemistry, 15, 9824-9829.
Huang, L., Romero, E., Ressmann, A. K., Rudroff, F., Hollmann, F., Fraaije, M. W., & Kara, S. (2017). Nicotinamide Adenine Dinucleotide-Dependent Redox-Neutral Convergent Cascade for Lactonizations with Type II Flavin-Containing Monooxygenase. Advanced Synthesis & Catalysis, 359(12), 2142-2148.
Fürst, M. J. L. J., Savino, S., Dudek, H. M., Gómez Castellanos, J. R., Gutiérrez de Souza, C., Rovida, S., Fraaije, M. W., & Mattevi, A. (2017). Polycyclic Ketone Monooxygenase from the Thermophilic Fungus Thermothelomyces thermophila: A Structurally Distinct Biocatalyst for Bulky Substrates. Journal of the American Chemical Society, 139(2), 627-630.
de Gonzalo, G., Fürst, M., & Fraaije, M. (2017). Polycyclic Ketone Monooxygenase (PockeMO): A Robust Biocatalyst for the Synthesis of Optically Active Sulfoxides. Catalysts, 7(10), 1-10. [288].
Pickl, M., Winkler, C. K., Glueck, S. M., Fraaije, M. W., & Faber, K. (2017). Rational Engineering of a Flavoprotein Oxidase for Improved Direct Oxidation of Alcohols to Carboxylic Acids. Molecules, 22(12), 1-8. [2205].
Ewing, T. A., Fraaije, M. W., Mattevi, A., & van Berkel, W. J. H. (2017). The VAO/PCMH flavoprotein family. Archives of Biochemistry and Biophysics, 632, 104-117.
Ewing, T. A., Nguyen, Q-T., Allan, R. C., Gygli, G., Romero, E., Binda, C., Fraaije, M. W., Mattevi, A., & van Berkel, W. J. H. (2017). Two tyrosine residues, Tyr-108 and Tyr-503, are responsible for the deprotonation of phenolic substrates in vanillyl-alcohol oxidase. The Journal of Biological Chemistry, 292(35), 14668-14679.


Nguyen, Q-T., Trinco, G., Binda, C., Mattevi, A., & Fraaije, M. W. (2017). Discovery and characterization of an F420-dependent glucose-6-phosphate dehydrogenase (Rh-FGD1) from Rhodococcus jostii RHA1. Applied Microbiology and Biotechnology, 101(7), 2831-2842.
Beyer, N., Kulig, J. K., Bartsch, A., Hayes, M. A., Janssen, D. B., & Fraaije, M. W. (2017). P450BM3 fused to phosphite dehydrogenase allows phosphite-driven selective oxidations. Applied Microbiology and Biotechnology, 101, 2319-2331.
de Gonzalo, G., Colpa, D. I., Habib, M. H. M., & Fraaije, M. W. (2016). Bacterial enzymes involved in lignin degradation. Journal of Biotechnology, 236, 110-119.
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