Development and use of engineered peptide deformylases in chemoenzymatic peptide synthesis
PhD ceremony: Ms. C. di Toma, 12.45 uur, Academiegebouw, Broerstraat 5, Groningen
Dissertation: Development and use of engineered peptide deformylases in chemoenzymatic peptide synthesis
Promotor(s): Prof. D.B. Janssen
Faculty: Mathematics and Natural Sciences
The goal of the research reported in this thesis is to explore the potential of the use of peptide deformylase in chemo-enzymatic peptide synthesis. PDF is suitable for the selective N-terminal deformylation of certain N-formyl-peptides without concurrent peptide bond hydrolysis. The challenge of applying PDF industrially prompted us to focus on the development of a new purification method to remove proteins with peptidase activity from the PDF. The use of enzyme engineering in combination with anion-exchange chromatography was proved to be a cost-efficient and industrially applicable purification method. Other aspects such as improving the stability and modifying the substrate specificity were also investigated. In order to broaden the specificity of PDF, we decided to use saturated mutagenesis at positions that play an important role in substrate recognition. A fast assay for the rapid selection of improved peptide deformylase variants in E. coli colonies was developed to analyze the constructed libraries. Various clones displaying new substrate specificity were recovered, sequenced and partially characterized.
Last modified: | 13 March 2020 01.00 a.m. |
More news
-
29 April 2024
Tactile sensors
Every two weeks, UG Makers puts the spotlight on a researcher who has created something tangible, ranging from homemade measuring equipment for academic research to small or larger products that can change our daily lives. That is how UG...
-
29 April 2024
Behind the scenes: how UG and Hanze UAS students are jointly developing a Mars rover
This year the students of the Makercie team are participating in the physical edition of the European Rover Challenge in Poland. Read more about the team and the collaboration between the RUG and Hanze UAS here.
-
23 April 2024
Nine MSCA Doctoral Network grants for FSE researchers
Nine researchers of the Faculty of Science and Engineering have received a Horizon Europe Marie Sklodowska Curie Doctoral Network grant.