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Corporate AcademyPart of University of Groningen

Corporate Academy

Safe Microbiological Techniques (SMT) course

This basic course teaches the students how to work safely with microorganisms and materials which can contain microorganisms and why you should do this. The course will lead to a certificate needed to be allowed to work in an ML-I laboratory. The course includes a theoretical and a practical part. The theoretical part consists of lectures and an e-learning.

Theoretical part:

Before the course, the RUG e-learning module (Biological Agents, module ML-I) will be followed and completed by passing a test. The module gives the student the basic knowledge for working with microorganisms.
The lectures will build on the e-learning and discuss examples from daily work and the application of law and regulations in institutions such as the RUG.

Practical part:

Techniques to safely deal with microorganisms. Working asceptically. Inoculating and purifying by sub culturing of bacteria cultures or samples. Determining the degree of contamination of air. Survival of a model microorganism on clothing. The formation of aerosols. Autoclave validation with a biological indicator. Test effectiveness of hand washing and hand disinfection. Disinfection of surfaces. Determination of bacteria in a mixture. Analysis of normal human microbiota present in students.

At the end of the course, the student is able to:
1.reproduce the rules for working according to SMT (Safe Microbiological Techniques) and shows that he/she can observe these SMT rules while working in the laboratory.
2.explain how micro-organisms spread.
3.explain why certain actions in the lab entail biological risks and how these risks can be mitigated.
4.explain how micro-organisms can be killed or removed and understands the potential pitfalls of disinfection and sterilization.
5.practise aseptical and purifying techniques


The course will take 3 days from 9:00-14:00


The course is planned from January 16th until January 18th.


The course will be given in the Linnaeusborg.


The course is free for members and employees form the RUG.

Last modified:21 March 2018 1.51 p.m.