Amperometric enzyme-based biosensors: refined bioanalytical tools for in vivo biomonitoring
|PhD ceremony:||Mr C.A. De Lima Braga Lopes Cordeiro|
|When:||January 12, 2018|
|Supervisors:||prof. dr. T.I.F.H. Cremers, prof. dr. B.H.C. Westerink|
|Where:||Academy building RUG|
|Faculty:||Science and Engineering|
Nowadays, in the most advanced clinical facilities, a new concept in medical care is emerging; Personalized Healthcare. This novel approach is based on patient individualization and relies on personalized health planning, enabled by personalized biomedical tools.
Ultimately, new biomedical tools, facilitated by advances in science and technology, will improve the ability to predict health risks, quantify the dynamics of disease development and allow implementation of individual therapeutic regimens. These new bioanalytical tools will accurately monitor (bio)chemical substance(s) (biomarker(s)), providing insightful personalized information on disease management.
In the case of diabetes, a worldwide leading cause of death, whose prevalence is predicted to largely increase, the key biomarker is glucose. Unfortunately, the existing biomedical tools for glucose (bio)monitoring are still far from enabling “truly” personalized diabetes management. Although several bioanalytical tools for glucose biomonitoring are currently under development, biosensors, in particular amperometric enzyme-based, are arguably the most promising ones.
In my thesis, I have studied in detail, the working mechanism of amperometric enzyme-based biosensors. Based on the added knowledge, I developed and characterized (in vitro and in vivo) refined amperometric enzyme-based biosensors for continuous glucose monitoring. Furthermore, I coupled them to wireless technology, aiming at better patient compliance. Additionally, I developed a multiplex biosensor, enabling simultaneous monitoring of glucose and other relevant biomarkers.
With this thesis, I tried to contribute to further increase the existing knowledge on biosensor technology. Also, I tried to emphasize the role of biosensors as a prospective tool for personalized biomonitoring, hopefully helping personalized healthcare to be one step closer to reality.