L. (Lisanne) Hameleers, PhD

10/2015 - 08/2018

Prof. B. Moerschbacher
WWU – IBBP, moersch uni-muenster.de

Westfälische Wilhelms-Universität Münster (WWU) – M.Sc. Biotechnology

Title of the master’s thesis written at the Institute of Plant Biology & Biotechnology:

“Heterologous expression & characterization of chitin modifying enzymes of Aspergillus niger in vitro and in vivo”

Heterologous protein expression:

• Various cloning techniques (gibson assembly, rolling circle, back2back)

• E. coli, P. pastoris, A. niger & HEK293T cells as expression systems
• Fermentation in 2-, 10-, 30- and 500 L scale

• Protein engineering by directed & random mutagenesis
• Screening of a mutant library in 96-well scale
• Enzyme activity assays, inhibition & kinetic studies
• Structural analysis with PyMol & homology modeling

• HPLC, LC-ESI-MS

• Production of monoclonal & chimeric antibodies
• Fluorescence-Activated Cell Sorting (FACS)
• Cell culture with HEK293T & VERO cells

• Agrobacterium tumefacience mediated transformation
• Experience in plant tissue culture

• Trained as project manager (according to §15 GenTSV)
  

10/2012 - 08/2015

Dr. G. Noll
WWU - IBBP, gnoll uni-muenster.de

Westfälische Wilhelms-Universität Münster (WWU) – B.Sc. Bioscience

Title of the bachelor’s thesis written at the institute of plant biology & biotechnology:

“Identification & molecular characterization of FT-homologs from Taraxacum ssp. ”

High school – St. Ursula Geilenkirchen

A-Levels (higher education entrance qualification) with focus on mathematics & biology

04- 05/2018

Prof. A.F.J. Ram      

LU-IBL, a.f.j.ram@biology.leidenuniv.nl

Universiteit Leiden (LU) – The Netherlands

Co-worker within the European Industrial Biotechnology project (FunChi)

Gene function analysis of chitin modifying enzymes by knock-out & overexpression experiments in Aspergillus niger

07 - 12/2016

Prof. S. B. Boscardin

USP - ICB, sbboscardin usp.br

Universidade de São Paulo (USP) - Brazil

Participant in the DAAD’s UNIBRAL program with a full study grant. Research at the institute of biomedicine in the department of immunology

Universidade da Coruña - Spain

Participant in the ERASMUS program with a focus on microbiology & applied plant physiology

05/2017 - 09/2018

04/2015 - 04/2016

Research assistant at Fraunhofer - IME Münster

• Assistance in running projects

Student association

• Organization of diverse events for students
• Member of examination boards
• Responsible for supporting students abroad

• Excellent skills with Microsoft Office Tools, Inkscape (graphic program), electronic-lab journal
  
• Basic skills in Python

• English - B2/C2
• Dutch   - B2
• Spanish/ Portuguese/ French - basic skills