How poppy flowers get those vibrant colours that entice insects

With bright reds and yellows – and even the occasional white – poppies are very bright and colourful. Their petals, however, are also very thin; they are made up of just three layers of cells. University of Groningen scientists Casper van der Kooi and Doekele Stavenga used microscopy and mathematical models describing how light interacts with petals to find out how the vibrant colours are created. The results will be included in a special edition of the Journal of Comparative Physiology A, which focuses on the relationship between insects and flowers.

Van der Kooi’s main research focus is the evolution of flower colour, and the interaction between flower colour and pollinators. This led him to investigate how petals produce their visual signals. He explains why the flowers of poppies (Papaver, Meconopsis and related species) are interesting: ‘The common poppy is an extreme case, it has very thin petals that nevertheless cause a very high scattering of light. Poppies also contain high concentrations of pigments.’

Jigsaw pieces

The researchers collected petals from different poppy species and studied their structures using different techniques. They discovered that the pigment was only present in the two outer cell layers and not in the middle layer. The pigmented cells had a fascinating shape, with many invaginations that made them look like complicated jigsaw pieces. ‘This creates many air-filled gaps between the cells, which cause the reflection of light on the cell/air boundary’, says Van der Kooi.

Furthermore, the petals contained huge amounts of pigment. ‘They are among the highest concentrations that I have ever measured in any flower.’ Indeed, the characteristic black markings at the centre of some poppy flowers are caused by extreme concentrations of red pigment. Van der Kooi concludes that dense pigmentation together with strong scattering cause the striking poppy colours in the red parts of the petal.

The new findings can be linked to previous work on poppy colour evolution. Intriguingly, poppies in the Middle East reflect no ultraviolet light, while the same species in Europe do. This difference may be due to their preferred pollinators. ‘In Europe, poppies are pollinated mostly by bees, which cannot see red very well; however, they will pick up ultraviolet.’ In contrast, poppies in the Middle East are pollinated by beetles that do see red colours. ‘Moreover, previous studies have shown that the black spots at the heart of some poppies mimic the presence of a female beetle. This is a way for the flowers to attract male beetles. A case of sexual mimicry, as occurs in other plants such as orchids’, explains Van der Kooi.

Evolution

The next question will be how these jigsaw-like cells and the air gaps that cause the efficient scattering have evolved. ‘These cell shapes are commonly present in leaves, so that might be a clue.’ Furthermore, results suggest that poppies evolved ultraviolet signals when they began growing in more northern regions. It makes the evolutionary history of these brightly coloured flowers an interesting object of study.

The paper by Van der Kooi and Stavenga will be included in a special edition of the Journal of Comparative Physiology A, edited by Friedrich Barth (University of Vienna). This special edition, with the title Insects and Flowers. New insights into an old partnership, is due to appear in print late this spring. The paper has already been published online.

Reference: CJ van der Kooi & DG Stavenga (2019) Vividly coloured poppy flowers due to dense pigmentation and strong scattering in thin petals. Journal of Comparative Physiology A, 2019. DOI: 10.1007/s00359-018-01313-1