Single-Molecule Nanopore Analysis

Single-molecule Enzymology

We are developing a new technology to investigate single-enzymatic reactions by using a protein nanopore. Single enzymes are covalently attached within the lumen of the pore and the enzymatic activity or the unfolding kinetics measured by the disruption of the ionic current through the pore.

Publications

Soskine M, Biesemans A, Maglia G. Single-Molecule Analyte Recognition with ClyA Nanopores Equipped with Internal Protein Adaptors. J Am Chem Soc. 6;137(17):5793-7 (2015). DOI

Soskine, M, Biesemans, A, De Maeyer, M, and Maglia, G. Tuning the Size and Properties of ClyA Nanopores Assisted by Directed Evolution. J Am Chem Soc (2013). DOI

DNA Analysis

Nanopores might be used to sequence or analyse DNA at low cost and high speed. We are studying the fundamental properties of DNA confinement into nanopores. We are also modifying ClyA nanopore for the analysis of double strand DNA.

Publications

Stoddart D, Franceschini L, Heron A, Bayley H, Maglia G. DNA stretching and optimization of nucleobase recognition in enzymatic nanopore sequencing. Nanotech. (2015) Feb 27;26(8):084002. DOI

Franceschini, L., Mikhailova, E., Bayley, H., and Maglia, G. Nucleobase recognition at alkaline pH and apparent pKa of single DNA bases immobilised within a biological nanopore. ChemComm 1;48(10):1520-2 (2012). DOI

Restrepo, M.R., Mikhailova, E., Bayley, H., and Maglia, G. Controlled translocation of individual DNA molecules through protein nanopores with engineered molecular brakes. Nano Lett 11(2):746-50 (2011). DOI

Maglia, G.Restrepo, M.R., Mikhailova, E., & Bayley, H. Enhanced translocation of single DNA molecules through alpha-hemolysin nanopores by manipulation of internal charge. Proc Natl Acad Sci USA 105 (50), 19720-19725 (2008). DOI