Emergent Functions
The emergence of an enzymatic function can reveal functional insights and allows engineering biological systems with enhanced properties. In the first example (a-b), we engineered an aHL nanopore to function as GroES, a protein that in complex with GroEL forms a two-stroke protein-folding nanomachine. In the second example (c-d), we engineered a biological nanopore to catalyse the selective transport of DNA across a biological membrane.
Publications
Ho CW, Van Meervelt V, Tsai KC, De Temmerman PJ, Mast J, and Maglia G. Engineering a nanopore with co-chaperonin function. Science Advances.11 Dec. Vol. 1, no. 11, e1500905 (2015) DOI: 10.1126/sciadv.1500905
Franceschini, L., Soskine, M, Biesemans, A, and Maglia, G. A nanopore machine promotes the vectorial transport of DNA across membranes. Nature Commun (2013). DOI: 10.1038/ncomms3415
Maglia, G., Heron, A. J., Hwang, W. L., Holden, M. A., Mikhailova, E., Li, Q., Cheley, S., and Bayley, H. Droplet networks with incorporated protein diodes show collective properties. Nature Nanotechnol 4 (7), 437-440 (2009). DOI
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