In the past decades there is growing interest in the application of therapeutic proteins for the treatment of various diseases. Polymer based injectable depot preparations that release proteins over a prolonged period of time may form an attractive alternative to the conventional injections of these drugs that usually require frequent administrations. Hot melt extrusion (HME) is a generally applied technology for the preparation of polymer based implants for the controlled release of drugs. However, HME has not been extensively explored for the controlled release of proteins yet. The technique may be harmful to the proteins due to their sensitive nature. The aim of the present study was to investigate the stabilization of proteins prior to, during and after encapsulation into polymer matrices by HME as well as during the release of the proteins from the matrices.
Sugar glass technology was applied to stabilize proteins and the mechanism of stabilization was studied. Various sugar glass stabilized proteins were encapsulated into novel biodegradable multiblock copolymers using HME, which could be accomplished at relatively low temperatures (thus favourable in view of protein stability). As a result of pre-stabilization using sugar glasses and the low process temperature, the proteins remained fully stable during the HME process. It was shown that the protein release from polymer depots can be tailored by co-incorporation of sugars as pore forming excipients but also by varying protein size and polymer composition. Polymer degradation rate appeared to be an important factor in the design of depot formulations. However, too fast degradation did lead to unwanted polymer-protein interactions, hampering protein release.
In conclusion, in this thesis the interplay between different polymer and protein related factors relevant for the development of a platform technology for successful stabilization, encapsulation and controlled release of proteins is described.
Ms M. (Milica) Stankovic
December 08, 2014
prof. dr. H.W. (Erik) Frijlink
Every two weeks, UG Makers puts the spotlight on a researcher who has created something tangible, ranging from homemade measuring equipment for academic research to small or larger products that can change our daily lives. That is how UG...
As a part of the Jantina Tammes School, the 'Language and AI' theme is an interdisciplinary initiative that aims to encourage collaboration among academics, PhD candidates, students, and industry representatives who share a keen interest in the...
Prof. Moniek Tromp has been appointed Captain of Science of the Chemistry Top Sector by the Minister of Economic Affairs and Climate Policy. As from 1 July 2023, she succeeded Prof. Bert Weckhuysen from Utrecht University.
The UG website uses functional and anonymous analytics cookies. Please answer the question of whether you want to accept
or reject other cookies (such as tracking cookies).
If no choice is made, only basic cookies will be stored. More information