PhD ceremony: Ms. V. Todde, 11.00 uur, Doopsgezinde Kerk, Oude Boteringestraat 33, Groningen
Dissertation: Organelle autophagy in yeast
Promotor(s): prof. I.J. van der Klei
Faculty: Mathematics and Natural Sciences
Organelles (e.g. peroxisomes, vacuoles etc) biogenesis and degradation (autophagy) is finely regulated. In this thesis we study different modes of organelle autophagy in yeast cells and the role of certain autophagic proteins. In Chapter 1 is presented an overview ofautophagy-related human diseases. In Chapter 2 we have shown: 1) that the peroxine Pex14p has no role in microautophagy; 2) the phosphorylation state of Pex14 may represent a signalling machinery for peroxisome degradation; 3) minor amounts of Pex14p are required for macropexophagy. In Chapter 3 Is shown that HpAtg19-like protein is not involved in peroxisome autophagy. In Chapter 4 We observed a dual location of S. cerevisiae Atg22 on the vacuole and peroxisome membranes without functions in peroxisomal biogenesis or autophagy. Chapter 5 describes the details of the autophagy phenotype of an S. cerevisiae atg22 mutant: 1) autophagic vesicles accumulates in the vacuole of nitrogen starved cells; 2) is a microautophagic phenotype; 3) is unrelated to leucine; 4) is showed a novel phenotype in oleate media (unrelated to leucine) similar for vacuolar lipase mutant atg15 and not to the protease pep4. Probably, the phenotype is related to malfunction in lipid degradation of microautophagic vesicles maybe related to a reduced vacuolar lipase activity in atg22 cells and possibly due to the accumulation of lipids. Chapter 6 describes a novel mode of lipid body (LB) degradation in vacuoles of S. cerevisiae cells via microautophagy. This process occurs and is stimulated in oleic acid growing cells independent by general autophagy.
Households that own their own home paid 776 euros to the municipality on average this year, which is 5.0 percent more than last year. 295 euros of this amount covers the immovable property tax (ozb), 283 euros are paid for waste collection levy, and...
1.5 miljoen euro voor De Donkerte van het Waddengebied
Three researchers from the University of Groningen have been awarded an ERC Advanced Grant. These three researchers are Prof. Gerard Roelfes (Stratingh Institute for Chemistry), Prof. Leon Koopmans (Kapteyn Astronomical Institute) and Prof. Erwin de...