VIDI

Generation of small designer protein catalysts with desired reaction specificity is a challenging task. A new and very promising approach is the rational discovery of new promiscuous reactions in existing enzymes, and exploiting this promiscuity to create tailor-made biocatalysts. The goal of this VIDI project is to develop small designer enzymes for non-natural carbonyl transformation reactions on the basis of the tautomerase superfamily scaffold through directed evolution, rational engineering, genome mining, and chemical synthesis.