Scheffers group Publications

Cavalca, L., Atlason, Ú. Á., Trofin, A., Ribeiro, C. M., Pavan, F. R., Deuss, P. J., & Scheffers, D.-J. (2024). Selectivity and Activity of Benzene-1,2,4-triol and its Dimers as Antimicrobial Compounds Against Xanthomonas citri subsp. citri. ChemPlusChem, Article e202300616. Advance online publication. https://doi.org/10.1002/cplu.202300616

Bramkamp, M., & Scheffers, D.-J. (2023). Bacterial membrane dynamics: Compartmentalization and repair. Molecular Microbiology, 120(4), Article 15077. https://doi.org/10.1111/mmi.15077

Ziylan, Z. S., de Putter, G. J., Roelofs, M., van Dijl, J. M., Scheffers, D. J., & Walvoort, M. T. C. (2023). Evaluation of Kdo-8-N₃ incorporation into lipopolysaccharides of various Escherichia coli strains. RSC Chemical Biology, 4(11), 884–893. https://doi.org/10.1039/d3cb00110e

Chakraborty, P., Oosterhuis, D., Bonsignore, R., Casini, A., Olinga, P., & Scheffers, D.-J. (2021). An organogold compound as potential antimicrobial agent against drug resistant bacteria: Initial mechanistic insights. ChemMedChem, 16(19), 3060-3070. https://doi.org/10.1002/cmdc.202100342

Polaquini, C. R., Marques, B. C., Ayusso, G. M., Morão, L. G., Sardi, J. C. O., Campos, D. L., Silva, I. C., Cavalca, L. B., Scheffers, D.-J., Rosalen, P. L., Pavan, F. R., Ferreira, H., & Regasini, L. O. (2021). Antibacterial activity of a new monocarbonyl analog of curcumin MAC 4 is associated with divisome disruption. Bioorganic Chemistry, 109, Article 104668. https://doi.org/10.1016/j.bioorg.2021.104668

Cavalca, L. B., Lahive, C. W., Gijsbers, F., Pavan, F. R., Scheffers, D.-J., & Deuss, P. J. (2021). Benzenetriol-Derived Compounds against Citrus Canker. Molecules, 26(5), Article 1436. https://doi.org/10.3390/molecules26051436

Li, Z., Chakraborty, P., de Vries, R. H., Song, C., Zhao, X., Roelfes, G., Scheffers, D.-J., & Kuipers, O. P. (2020). Characterization of two relacidines belonging to a novel class of circular lipopeptides that act against Gram-negative bacterial pathogens. Environmental Microbiology, 22(12), 5125-5136. https://doi.org/10.1111/1462-2920.15145

Zielińska, A., Savietto, A., de Sousa Borges, A., Martinez, D., Berbon, M., Roelofsen, J. R., Hartman, A. M., de Boer, R., Van der Klei, I. J., Hirsch, A. K., Habenstein, B., Bramkamp, M., & Scheffers, D.-J. (2020). Flotillin-mediated membrane fluidity controls peptidoglycan synthesis and MreB movement. eLife, 9, 1-21. Article e57179. https://doi.org/10.7554/eLife.57179

Morao, L. G., Lorenzoni, A. S. G., Chakraborty, P., Ayusso, G. M., Cavalca, L. B., Santos, M. B., Marques, B. C., Dilarri, G., Zamuner, C., Regasini, L. O., Ferreira, H., & Scheffers, D.-J. (2020). Investigating the Modes of Action of the Antimicrobial Chalcones BC1 and T9A. Molecules, 25(20), Article 4596. https://doi.org/10.3390/molecules25204596

Li, Z., de Vries, R. H., Chakraborty, P., Song, C., Zhao, X., Scheffers, D.-J., Roelfes, G., & Kuipers, O. P. (2020). Novel modifications of nonribosomal peptides from Brevibacillus laterosporus MG64 and investigation of their mode of action. Applied and environmental microbiology, 86(24), 1-14. Article e01981-20. https://doi.org/10.1128/AEM.01981-20

Morales Angeles, D., & Scheffers, D.-J. (2020). The Cell Wall of Bacillus subtilis. Current Issues in Molecular Biology, 41, 539-596. https://doi.org/10.21775/cimb.041.539

Morales Angeles, D., Macia Valero, A., Bohorquez Suarez, L., & Scheffers, D.-J. (2020). The PASTA domains of Bacillus subtilis PBP2B stabilize the interaction of PBP2B with DivIB. Microbiology, 166(9), 826–836. Article 000957. https://doi.org/10.1099/mic.0.000957

Polaquini, C. R., Morão, L. G., Nazaré, A. C., Torrezan, G. S., Dilarri, G., Cavalca, L. B., Campos, D. L., Silva, I. C., Pereira, J. A., Scheffers, D.-J., Duque, C., Pavan, F. R., Ferreira, H., & Regasini, L. O. (2019). Antibacterial activity of 3,3'-dihydroxycurcumin (DHC) is associated with membrane perturbation. Bioorganic Chemistry, 90, Article 103031. https://doi.org/10.1016/j.bioorg.2019.103031

Morão, L. G., Polaquini, C. R., Kopacz, M., Torrezan, G. S., Ayusso, G. M., Dilarri, G., Cavalca, L. B., Zielińska, A., Scheffers, D.-J., Regasini, L. O., & Ferreira, H. (2019). A simplified curcumin targets the membrane of Bacillus subtilis. MicrobiologyOpen, 8(4), Article 683. https://doi.org/10.1002/mbo3.683

Kopacz, M. M., Lorenzoni, A. S. G., Polaquini, C. R., Regasini, L. O., & Scheffers, D.-J. (2019). Purification and characterization of FtsZ from the citrus canker pathogen Xanthomonas citri subsp. citri. MicrobiologyOpen, 8(5), Article e00706. https://doi.org/10.1002/mbo3.706

Savietto, A., Polaquini, C. R., Kopacz, M., Scheffers, D.-J., Marques, B. C., Regasini, L. O., & Ferreira, H. (2018). Antibacterial activity of monoacetylated alkyl gallates against Xanthomonas citri subsp citri. Archives of Microbiology, 200(6), 929-937. https://doi.org/10.1007/s00203-018-1502-6

Nazaré, A. C., Polaquini, C. R., Cavalca, L. B., Anselmo, D. B., Saiki, M. D. F. C., Monteiro, D. A., Zielinska, A., Rahal, P., Gomes, E., Scheffers, D.-J., Ferreira, H., & Regasini, L. O. (2018). Design of Antibacterial Agents: Alkyl Dihydroxybenzoates against Xanthomonas citri subsp. citri. International Journal of Molecular Sciences, 19(10), 1-15. https://doi.org/10.3390/ijms19103050

Dockerty, P., Edens, J. G., Tol, M. B., Morales Angeles, D., Domenech Pena, A., Liu, Y., Hirsch, A. K. H., Veening, J.-W., Scheffers, D.-J., & Witte, M. D. (2017). Bicyclic enol cyclocarbamates inhibit penicillin-binding proteins. Organic & Biomolecular Chemistry, 15(4), 894-910. https://doi.org/10.1039/c6ob01664b

Król, E., de Sousa Borges, A., Kopacz, M., & Scheffers, D.-J. (2017). Metal-dependent SpoIIE oligomerization stabilizes FtsZ during asymmetric division in Bacillus subtilis. PLoS ONE, 12(3), Article e0174713. https://doi.org/10.1371/journal.pone.0174713

Morales Angeles, D., Liu, Y., Hartman, A. M., Borisova, M., de Sousa Borges, A., de Kok, N., Beilharz, K., Veening, J.-W., Mayer, C., Hirsch, A. K. H., & Scheffers, D.-J. (2017). Pentapeptide-rich peptidoglycan at the Bacillus subtilis cell-division site. Molecular Microbiology, 104(2), 319-333. https://doi.org/10.1111/mmi.13629

Soibelmann Glock Lorenzoni, A., Dantas, G., Bergsma, T., Ferreira, H., & Scheffers, D. (2017). Xanthomonas citri MinC Oscillates from Pole to Pole to Ensure Proper Cell Division and Shape. Frontiers in Microbiology, 8, 1-12. Article 1352. https://doi.org/10.3389/fmicb.2017.01352

de Sousa Borges, A., & Scheffers, D.-J. (2016). Bacterial dynamin as a membrane puncture repair kit. Environmental Microbiology, 18(8), 2298-2301. https://doi.org/10.1111/1462-2920.13218

Pereira, A. R., Hsin, J., Król, E., Tavares, A. C., Flores, P., Hoiczyk, E., Ng, N., Dajkovic, A., Brun, Y. V., VanNieuwenhze, M. S., Roemer, T., Carballido-Lopez, R., Scheffers, D.-J., Huang, K. C., & Pinho, M. G. (2016). FtsZ-Dependent Elongation of a Coccoid Bacterium. mBio, 7(5), 1-9. Article e00908-16 . https://doi.org/10.1128/mBio.00908-16

Trip, E. N., & Scheffers, D.-J. (2015). A 1 MDa protein complex containing critical components of the Escherichia coli divisome. Scientific Reports, 5, Article 18190. https://doi.org/10.1038/srep18190

Krol, E., de Sousa Borges, A., da Silva, I., Polaquini, C., Regasini, L., Ferreira, H., & Scheffers, D. (2015). Antibacterial activity of alkyl gallates is a combination of direct targeting of FtsZ and permeabilization of bacterial membranes. Frontiers in Microbiology, 6, Article 390. https://doi.org/10.3389/fmicb.2015.00390

Silva, I., Pavan, F. R., Polaquini, C., Regasini, L. O., Scheffers, D. .-J., & Ferreira, H. (2015). Assessment of cyto/genotoxicity of the antibacterial heptyl gallate in HepG2 cells and the possible target in bacteria. Toxicology Letters, 238(2, Supplement), S328-S328. https://doi.org/10.1016/j.toxlet.2015.08.935

Tol, M. B., Angeles, D. M., & Scheffers, D.-J. (2015). In vivo cluster formation of nisin and Lipid II is correlated with membrane depolarization. Antimicrobial Agents and Chemotherapy, 59(6), 3683-3686. https://doi.org/10.1128/AAC.04781-14

Scheffers, D.-J., & Tol, M. B. (2015). LipidII: Just Another Brick in the Wall? PLoS Pathogens, 11(12), Article e1005213. https://doi.org/10.1371/journal.ppat.1005213

de Sousa Borges, A., de Keyzer, J., Driessen, A. J. M., & Scheffers, D.-J. (2015). The Escherichia coli membrane protein insertase YidC assists in the biogenesis of Penicillin Binding Proteins. Journal of Bacteriology, 197, 1444-1450. https://doi.org/10.1128/JB.02556-14

Geng, Y., Keyzer, J. D., Scheffers, D.-J., & Driessen, A. J. M. (2014). Defining the Region of Bacillus subtilis SpoIIIJ That Is Essential for Its Sporulation-Specific Function. Journal of Bacteriology, 196(7), 1318-1324. https://doi.org/10.1128/JB.01084-13

Trip, E. N., Veening, J.-W., Stewart, E. J., Errington, J., & Scheffers, D.-J. (2013). Balanced transcription of cell division genes in Bacillus subtilis as revealed by single cell analysis. Environmental Microbiology, 15(12), 3196-3209. https://doi.org/10.1111/1462-2920.12148

Boekema, E. J., Scheffers, D.-J., van Bezouwen, L. S., Bolhuis, H., & Folea, I. M. (2013). Focus on Membrane Differentiation and Membrane Domains in the Prokaryotic Cell. Journal of Molecular Microbiology and Biotechnology, 23(4-5), 345-356. https://doi.org/10.1159/000351361

Król, E., & Scheffers, D.-J. (2013). FtsZ Polymerization Assays: Simple Protocols and Considerations. Journal of Visualized Experiments, (81), e50844-1-e50844-13. https://doi.org/10.3791/50844

Ballesteros-Plaza, D., Holguera, I., Scheffers, D.-J., Salas, M., & Munoz-Espin, D. (2013). Phage phi 29 protein p1 promotes replication by associating with the FtsZ ring of the divisome in Bacillus subtilis. Proceedings of the National Academy of Sciences of the United States of America, 110(30), 12313-12318. https://doi.org/10.1073/pnas.1311524110

Lages, M. C. A., Beilharz, K., Angeles, D. M., Veening, J.-W., & Scheffers, D.-J. (2013). The localization of key Bacillus subtilis penicillin binding proteins during cell growth is determined by substrate availability. Environmental Microbiology, 15(12), 3272-3281. https://doi.org/10.1111/1462-2920.12206

Krol, E., van Kessel, S. P., van Bezouwen, L. S., Kumar, N., Boekema, E. J., & Scheffers, D.-J. (2012). Bacillus subtilis SepF Binds to the C-Terminus of FtsZ. PLoS ONE, 7(8), Article e43293. https://doi.org/10.1371/journal.pone.0043293

Yu, Z., Bekker, M., Tramonti, A., Cook, G. M., van Ulsen, P., Scheffers, D.-J., de Mattos, J. T., De Biase, D., & Luirink, J. (2011). Activators of the Glutamate-Dependent Acid Resistance System Alleviate Deleterious Effects of YidC Depletion in Escherichia coli. Journal of Bacteriology, 193(6), 1308-1316. https://doi.org/10.1128/JB.01209-10

Guendogdu, M. E., Kawai, Y., Pavlendova, N., Ogasawara, N., Errington, J., Scheffers, D.-J., Hamoen, L. W., & Gündoğdu, M. E. (2011). Large ring polymers align FtsZ polymers for normal septum formation. EMBO Journal, 30(3), 617-626. https://doi.org/10.1038/emboj.2010.345

Fernandes de Oliveira, I. F., Sousa Borges, A. D., Kooij, V., Bartosiak-Jentys, J., Luirink, J., & Scheffers, D.-J. (2010). Characterization of ftsZ Mutations that Render Bacillus subtilis Resistant to MinC. PLoS ONE, 5(8), Article e12048. https://doi.org/10.1371/journal.pone.0012048

Ribeiro, J. P., Palczewska, M., André, S., Cañada, F. J., Gabius, H.-J., Jiménez-Barbero, J., Mellström, B., Naranjo, J. R., Scheffers, D.-J., & Groves, P. (2010). Diffusion nuclear magnetic resonance spectroscopy detects substoichiometric concentrations of small molecules in protein samples. Analytical Biochemistry, 396(1), 117-123. https://doi.org/10.1016/j.ab.2009.09.001

Pop, O. I., Soprova, Z., Koningstein, G., Scheffers, D.-J., Ulsen, P. V., Wickström, D., Gier, J.-W. D., & Luirink, J. (2009). YidC is required for the assembly of the MscL homopentameric pore. The FEBS Journal, 276(17), 4891-4899. https://doi.org/10.1111/j.1742-4658.2009.07188.x

Formstone, A., Carballido-López, R., Noirot, P., Errington, J., & Scheffers, D.-J. (2008). Localization and Interactions of Teichoic Acid Synthetic Enzymes in Bacillus subtilis. Journal of Bacteriology, 190(5), 1812-1821. https://doi.org/10.1128/JB.01394-07

Scheffers, D.-J. (2008). The effect of MinC on FtsZ polymerization is pH dependent and can be counteracted by ZapA. FEBS Letters, 582(17), 2601-2608. https://doi.org/10.1016/j.febslet.2008.06.038

Scheffers, D.-J. (2007). Cell wall growth during elongation and division: one ring to bind them? Molecular Microbiology, 64(4), 877-880. https://doi.org/10.1111/j.1365-2958.2007.05731.x

Scheffers, D.-J., Robichon, C., Haan, G. J., Blaauwen, T. D., Koningstein, G., Bloois, E. V., Beckwith, J., & Luirink, J. (2007). Contribution of the FtsQ Transmembrane Segment to Localization to the Cell Division Site. Journal of Bacteriology, 189(20), 7273-7280. https://doi.org/10.1128/JB.00723-07

Scheffers, D.-J., & Pinho, M. G. (2005). Bacterial Cell Wall Synthesis: New Insights from Localization Studies. Microbiology and Molecular Biology Reviews, 69(4), 585-607. https://doi.org/10.1128/MMBR.69.4.585-607.2005

Scheffers, D.-J. (2005). Dynamic localization of penicillin-binding proteins during spore development in Bacillus subtilis. Microbiology, 151(3), 999-1012. https://doi.org/10.1099/mic.0.27692-0

Scheffers, D.-J., & Errington, J. (2004). PBP1 Is a Component of the Bacillus subtilis Cell Division Machinery. Journal of Bacteriology, 186(15), 5153-5156. https://doi.org/10.1128/JB.186.15.5153-5156.2004

Koppelman, CM., Aarsman, MEG., Postmus, J., Pas, E., Muijsers, AO., Scheffers, DJ., Nanninga, N., & den Blaauwen, T. (2004). R174 of Escherichia coli FtsZ is involved in membrane interaction and protofilament bundling, and is essential for cell division. Molecular Microbiology, 51(3), 645-657. https://doi.org/10.1046/j.1365-2958.2003.03876.x

Scheffers, D.-J., Jones, L. J. F., & Errington, J. (2004). Several distinct localization patterns for penicillin-binding proteins in Bacillus subtilis. Molecular Microbiology, 51(3), 749-764.

Errington, J., Daniel, R. A., & Scheffers, D.-J. (2003). Cytokinesis in Bacteria. Microbiology and Molecular Biology Reviews, 67(1), 52-65. https://doi.org/10.1128/MMBR.67.1.52-65.2003

Scheffers, D.-J., de Wit, J. G., Blaauwen, T. D., & Driessen, A. J. M. (2002). GTP hydrolysis of cell division protein FtsZ: Evidence that the active site is formed by the association of monomers: Evidence that the Active Site Is Formed by the Association of Monomers. Biochemistry, 41(2), 521 - 529. https://doi.org/10.1021/bi011370i

Scheffers, D.-J., & Driessen, A. J. M. (2002). Immediate GTP hydrolysis upon FtsZ polymerization. Molecular Microbiology, 43(6), 1517 - 1521. https://doi.org/10.1046/j.1365-2958.2002.02828.x

Scheffers, D.-J., de Wit, J. G., Blaauwen, T. D., & Driessen, A. J. M. (2001). Substitution of a conserved aspartate allows cation-induced polymerization of FtsZ. FEBS Letters, 494(1-2), 34 - 37. https://doi.org/10.1016/S0014-5793(01)02310-9

Scheffers, D.-J., & Driessen, A. J. M. (2001). The polymerization mechanism of the bacterial cell division protein FtsZ. FEBS Letters, 506(1), 6 - 10. https://doi.org/10.1016/S0014-5793(01)02855-1

Scheffers, D. J., den Blaauwen, T., & Driessen, A. J. (2000). Non-hydrolysable GTP-gamma-S stabilizes the FtsZ polymer in a GDP-bound state. Molecular Microbiology, 35(5), 1211-1219. https://doi.org/10.1046/j.1365-2958.2000.01791.x