Optical Microscopy & Spectroscopy

Cellular imaging: we use a dual-color confocal microscope for time-lapse imaging of dynamic processes in cells or GUVs.
Protein & lipid diffusion: the diffusion of lipids^1,2 and proteins ^2,3 is measured by fluorescence correlation spectroscopy (FCS) and fluorescence-recovery after photobleached (FRAP)-based methods.
Transport through membrane pores: a method termed dual-color fluorescence burst analysis (DCFBA) has bee developed to probe (macro)molecular transport through mechanosensitive channels and peptide pores. Selective-FRAP is used to probe the in situ transport through the yeast nuclear pore complex.

Kahya, N., Scherfeld, D., Bacia, K., Poolman, B., and Schwille, P. (2003)
Probing lipid mobility of raft-exhibiting model membranes by Fluorescence Correlation Spectroscopy.
J. Biol. Chem., 278, 28109-28115.
Doeven, M.K., Folgering, J.H.A., Krasnikov, V., Geertsma, E.R., van den Bogaart, G., and Poolman, B. (2005) Distribution, Lateral Mobility and Function of Membrane Proteins Incorporated into Giant Unilamellar Vesicles. Biophys. J., 88, 1134-1142.
van den Bogaart, G., Hermans, N., Krasnikov, V., and Poolman, B. (2007) Protein mobility and diffusive barriers in Escherichia coli: consequences of osmotic stress. Molec. Microbiol., 64, 858-871 .

Last modified:

03 October 2012 10.04 a.m.