Optical Microscopy & Spectroscopy
- Cellular imaging: we use a dual-color confocal microscope for time-lapse imaging of dynamic processes in cells or GUVs.
- Protein & lipid diffusion: the diffusion of lipids1,2 and proteins 2,3 is measured by fluorescence correlation spectroscopy (FCS) and fluorescence-recovery after photobleached (FRAP)-based methods.
- Transport through membrane pores: a method termed dual-color fluorescence burst analysis (DCFBA) has bee developed to probe (macro)molecular transport through mechanosensitive channels and peptide pores. Selective-FRAP is used to probe the in situ transport through the yeast nuclear pore complex.
- Kahya, N., Scherfeld, D., Bacia, K., Poolman, B., and Schwille, P. (2003)
Probing lipid mobility of raft-exhibiting model membranes by Fluorescence Correlation Spectroscopy.
J. Biol. Chem., 278, 28109-28115.
- Doeven, M.K., Folgering, J.H.A., Krasnikov, V., Geertsma, E.R., van den Bogaart, G., and Poolman, B. (2005) Distribution, Lateral Mobility and Function of Membrane Proteins Incorporated into Giant Unilamellar Vesicles. Biophys. J., 88, 1134-1142.
- van den Bogaart, G., Hermans, N., Krasnikov, V., and Poolman, B. (2007) Protein mobility and diffusive barriers in Escherichia coli: consequences of osmotic stress. Molec. Microbiol., 64, 858-871 .
|Last modified:||03 October 2012 12.04 p.m.|