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Over onsMedische WetenschappenResearchDepartment of GeneticsGenome Analysis FacilityServices
University Medical Center Groningen

DNA sequencing

Next-generation DNA sequencing allows the comparison of the genetic content among samples and the identification of germline and somatic genetic variants, such as single nucleotide polymorphisms (SNPs), insertions and deletions, and copy number variants (CNVs). We can advise you on choosing the most suitable DNA sequencing method from our portfolio to answer your research question.

We support the following DNAseq applications:

(1) Exome-sequencing

We offer exome sequencing using Agilent SureSelectXT Human All Exon V6 capturing kits. DNA samples are processed with a liquid handler (microplate-format). After finishing the sequence library preparation and quality control, samples are loaded on to the Illumina NextSeq500 for paired-end sequencing. The number of generated reads will be determined in advance in consultation with the researcher.

(2) Targeted DNA sequencing of selected gene panels

We offer several selected gene panels for research purposes. DNA samples are processed with a liquid handler (microplate-format) into sequence libraries. After quantification of the library and quality control, samples are loaded on to the Illumina NextSeq500 for paired-end sequencing. The number of generated reads will be determined in advance in consultation with the researcher.

Conditions and disclaimers for DNA sequencing

We ask a failure fee for each project to allow for technical problems during the experiment.

To ensure optimal conditions for library preparation and high quality sequencing data, DNA samples have to be checked by Nanodrop for quantity and quality/integrity. We can facilitate this QC measurement for you. Quality criteria of DNA samples will be discussed in advance and described in a contract, which includes our terms of agreement and disclaimers. Under these conditions, we will make a second attempt to prepare the library if the first attempt fails. For this you will not incur additional costs. For samples of lower quality, we will only make one attempt, at the researcher’s own risk. If this fails, a second attempt will only be made if paid by the researcher. If the project is cancelled at any point by the researcher, all costs made up to that point will be billed to the researcher.

The number of reads we generate for your NGS library will be determined in advance of the experiment. If this threshold is not obtained because of technical problems, we will re-run the samples on a second sequencing run.

We can advise you on choosing the most suitable DNA sequencing method from our portfolio to answer your research question. However, downstream analysis is not part of our standard service. Should you need help, we can refer you to the Genomics Coordination Centre for more advanced data analysis services, such as mapping reads and variant calling.

Last modified:11 September 2017 4.47 p.m.