Publications

Gilsbach, B. K., Ho, F. Y., Riebenbauer, B., Zhang, X., Guaitoli, G., Kortholt, A., & Gloeckner, C. J. (2024). Intramolecular feedback regulation of the LRRK2 Roc G domain by a LRRK2 kinase-dependent mechanism. eLife, 12, Article RP91083. https://doi.org/10.7554/eLife.91083

Iannotta, L., Fasiczka, R., Favetta, G., Zhao, Y., Giusto, E., Dall'Ara, E., Wei, J., Ho, F. Y., Ciriani, C., Cogo, S., Tessari, I., Iaccarino, C., Liberelle, M., Bubacco, L., Taymans, J.-M., Manzoni, C., Kortholt, A., Civiero, L., Hilfiker, S., ... Greggio, E. (2024). PAK6 rescues pathogenic LRRK2-mediated ciliogenesis and centrosomal cohesion defects in a mutation-specific manner. Cell death & disease, 15(10), Article 752. https://doi.org/10.1038/s41419-024-07124-4

Ahuja, G., Arauz, Y. L. A., van Heuvelen, M. J. G., Kortholt, A., Oroszi, T., & van der Zee, E. A. (2024). The effects of whole-body vibration therapy on immune and brain functioning: current insights in the underlying cellular and molecular mechanisms. Frontiers in Neurology, 15, Article 1422152. https://doi.org/10.3389/fneur.2024.1422152

Oun, A., Hoeksema, E., Soliman, A., Brouwer, F., García-Reyes, F., Pots, H., Trombetta-Lima, M., Kortholt, A., & Dolga, A. M. (2023). Characterization of Lipopolysaccharide Effects on LRRK2 Signaling in RAW Macrophages. International Journal of Molecular Sciences, 24(2), Article 1644. https://doi.org/10.3390/ijms24021644

Pathak, P., Alexander, K. K., Helton, L. G., Kentros, M., LeClair, T. J., Zhang, X., Ho, F. Y., Moore, T. T., Hall, S., Guaitoli, G., Gloeckner, C. J., Kortholt, A., Rideout, H., & Kennedy, E. J. (2023). Doubly Constrained C-terminal of Roc (COR) Domain-Derived Peptides Inhibit Leucine-Rich Repeat Kinase 2 (LRRK2) Dimerization. ACS chemical neuroscience, 14(11), 1971-1980. Article 00259. https://doi.org/10.1021/acschemneuro.3c00259

Zhang, X., & Kortholt, A. (2023). LRRK2 Structure-Based Activation Mechanism and Pathogenesis. Biomolecules, 13(4), Article 612. https://doi.org/10.3390/biom13040612

Iannotta, L., Emanuele, M., Favetta, G., Tombesi, G., Vandewynckel, L., Lara Ordóñez, A. J., Saliou, J.-M., Drouyer, M., Sibran, W., Civiero, L., Nichols, R. J., Athanasopoulos, P. S., Kortholt, A., Chartier-Harlin, M.-C., Greggio, E., & Taymans, J.-M. (2023). PAK6-mediated phosphorylation of PPP2R2C regulates LRRK2-PP2A complex formation. Frontiers in Molecular Neuroscience, 16, Article 1269387. https://doi.org/10.3389/fnmol.2023.1269387

Sabogal-Guáqueta, A. M., Marmolejo-Garza, A., Trombetta-Lima, M., Oun, A., Hunneman, J., Chen, T., Koistinaho, J., Lehtonen, S., Kortholt, A., Wolters, J. C., Bakker, B. M., Eggen, B. J. L., Boddeke, E., & Dolga, A. (2023). Species-specific metabolic reprogramming in human and mouse microglia during inflammatory pathway induction. Nature Communications, 14(1), Article 6454. https://doi.org/10.1038/s41467-023-42096-7

Marchand, A., Sarchione, A., Athanasopoulos, P., Roy, H. B.-L., Goveas, L., Romain, MAGNEZ., Drouyer, M., Emanuele, M., Ho, F. Y., Liberelle, M., Melnyk, P., Nicolas, LEBEGUE., Thuru, X., Nichols, R. J., Greggio, E., kortholt, A., Galli, T., chartier-harlin, M., & Taymans, J.-M. (2022). A Phosphosite Mutant Approach on LRRK2 Links Phosphorylation and Dephosphorylation to Protective and Deleterious Markers, Respectively. Cells, 11(6), Article 1018. https://doi.org/10.3390/cells11061018

Xu, X., Pots, H., Gilsbach, B. K., Parsons, D., Veltman, D. M., Ramachandra, S. G., Li, H., Kortholt, A., & Jin, T. (2022). C2GAP2 is a common regulator of Ras signaling for chemotaxis, phagocytosis, and macropinocytosis. Frontiers in Immunology, 13, Article 1075386. https://doi.org/10.3389/fimmu.2022.1075386

Skakun, V. V., Yatskou, M. M., Nederveen-Schippers, L., Kortholt, A., & Apanasovich, V. V. (2022). Component Analysis of Photon Counting Histograms in Fluorescence Fluctuation Spectroscopy Experiments. Journal of applied spectroscopy, 89(5), 930-939. https://doi.org/10.1007/s10812-022-01450-1

Rideout, H., Greggio, E., Kortholt, A., & Nichols, R. J. (2022). Editorial: LRRK2-Fifteen Years From Cloning to the Clinic. Frontiers in Neuroscience, 16, Article 880914. https://doi.org/10.3389/fnins.2022.880914

Oun, A., Soliman, A., Trombetta-Lima, M., Tzepapadaki, A., Tsagkari, D., Kortholt, A., & Dolga, A. M. (2022). LRRK2 protects immune cells against erastin-induced ferroptosis. Neurobiology of Disease, 175, Article 105917. https://doi.org/10.1016/j.nbd.2022.105917

Singh, R. K., Soliman, A., Guaitoli, G., Störmer, E., von Zweydorf, F., Dal Maso, T., Oun, A., Van Rillaer, L., Schmidt, S. H., Chatterjee, D., David, J. A., Pardon, E., Schwartz, T. U., Knapp, S., Kennedy, E. J., Steyaert, J., Herberg, F. W., Kortholt, A., Gloeckner, C. J., & Versées, W. (2022). Nanobodies as allosteric modulators of Parkinson's disease-associated LRRK2. Proceedings of the National Academy of Sciences of the United States of America, 119(9), Article e2112712119. https://doi.org/10.1073/pnas.2112712119

Arenales Arauz, Y. L., Ahuja, G., Kamsma, Y. P. T., Kortholt, A., van der Zee, E. A., & van Heuvelen, M. J. G. (2022). Potential of Whole-Body Vibration in Parkinson's disease: A systematic review and meta-analysis of human and animal studies. Biology, 11(8), Article 11081238. https://doi.org/10.3390/biology11081238

Cankara, F. N., Kuş, M. S., Günaydın, C., Şafak, S., Bilge, S. S., Ozmen, O., Tural, E., & Kortholt, A. (2022). The beneficial effect of salubrinal on neuroinflammation and neuronal loss in intranigral LPS-induced hemi-Parkinson disease model in rats. Immunopharmacology and Immunotoxicology, 44(2), 168-177. https://doi.org/10.1080/08923973.2021.2023174

Oun, A., Sabogal-Guaqueta, A. M., Galuh, S., Alexander, A., Kortholt, A., & Dolga, A. M. (2022). The multifaceted role of LRRK2 in Parkinson's disease: From human iPSC to organoids. Neurobiology of Disease, 173, Article 105837. https://doi.org/10.1016/j.nbd.2022.105837

Cogo, S., Ho, F. Y., Tosoni, E., Tomkins, J. E., Tessari, I., Iannotta, L., Montine, T. J., Manzoni, C., Lewis, P. A., Bubacco, L., Chartier Harlin, M.-C., Taymans, J.-M., Kortholt, A., Nichols, J., Cendron, L., Civiero, L., & Greggio, E. (2022). The Roc domain of LRRK2 as a hub for protein-protein interactions: a focus on PAK6 and its impact on RAB phosphorylation. Brain Research, 1778, Article 147781. https://doi.org/10.1016/j.brainres.2022.147781

Rosenbusch, I., Oun, A., Sanislav, O., Lay, S. T., Keizer-Gunnink, I., Annesley, S. J., Fisher, P. R., Dolga, A., & Kortholt, A. (2021). A conserved role for LRRK2 and Roco proteins in the regulation of mitochondrial activity. Frontiers in Cell and Developmental Biology, 9, Article 734554. https://doi.org/10.3389/fcell.2021.734554

Helton, L. G., Soliman, A., von Zweydorf, F., Kentros, M., Manschwetus, J. T., Hall, S., Gilsbach, B., Ho, F. Y., Athanasopoulos, P. S., Singh, R. K., LeClair, T. J., Versées, W., Raimondi, F., Herberg, F. W., Gloeckner, C. J., Rideout, H., Kortholt, A., & Kennedy, E. J. (2021). Allosteric Inhibition of Parkinson's-Linked LRRK2 by Constrained Peptides. ACS chemical biology, 16(11), 2326–2338. https://doi.org/10.1021/acschembio.1c00487

Nederveen-Schippers, L. M., Pathak, P., Keizer-Gunnink, I., Westphal, A. H., van Haastert, P. J. M., Borst, J. W., Kortholt, A., & Skakun, V. (2021). Combined FCS and PCH analysis to quantify protein dimerization in living cells. International Journal of Molecular Sciences, 22(14), Article 7300. https://doi.org/10.3390/ijms22147300

van Haastert, P. J. M., Keizer-Gunnink, I., Pots, H., Ortiz-Mateos, C., Veltman, D., van Egmond, W., & Kortholt, A. (2021). Forty-five years of cGMP research in Dictyostelium: Understanding the regulation and function of the cGMP pathway for cell movement and chemotaxis. Molecular Biology of the Cell, 32(20), 1-14. Article mbcE21040171. https://doi.org/10.1091/mbc.E21-04-0171

Wojewska, D. N., & Kortholt, A. (2021). Lrrk2 targeting strategies as potential treatment of parkinson’s disease. Biomolecules, 11(8), Article 1101. https://doi.org/10.3390/biom11081101

Xu, X., Bhimani, S., Pots, H., Wen, X., Jeon, T. J., Kortholt, A., & Jin, T. (2021). Membrane Targeting of C2GAP1 Enables Dictyostelium discoideum to Sense Chemoattractant Gradient at a Higher Concentration Range. Frontiers in Cell and Developmental Biology, 9, Article 725073. https://doi.org/10.3389/fcell.2021.725073

van Haastert, P. J. M. (2021). Short- and long-term memory of moving amoeboid cells. PLoS ONE, 16(2), Article e0246345. https://doi.org/10.1371/journal.pone.0246345

Konstantinidou, M., Oun, A., Pathak, P., Zhang, B., Wang, Z., ter Brake, F., Dolga, A., Kortholt, A., & Doemling, A. (2021). The tale of proteolysis targeting chimeras (PROTACs) for leucine-rich repeat kinase 2 (LRRK2). ChemMedChem, 16(6), 959-965. https://doi.org/10.1002/cmdc.202000872

Soliman, A., Cankara, F. N., & Kortholt, A. (2020). Allosteric inhibition of LRRK2, where are we now. Biochemical Society Transactions, 48(5), 2185-2194. Article BST20200424. https://doi.org/10.1042/BST20200424

Leemans, M., Galicia, C., Deyaert, E., Daems, E., Krause, L., Paesmans, J., Pardon, E., Steyaert, J., Kortholt, A., Sobott, F., Klostermeier, D., & Versées, W. (2020). Allosteric modulation of the GTPase activity of a bacterial LRRK2 homolog by conformation-specific Nanobodies. Biochemical Journal, 477(7), 1203-1218. https://doi.org/10.1042/BCJ20190843

Yatskou, M. M., Skakun, V. V., Nederveen-Schippers, L., Kortholt, A., & Apanasovich, V. V. (2020). Complex Analysis of Fluorescence Intensity Fluctuations of Molecular Compounds. Journal of applied spectroscopy, 87(4), 685-692. https://doi.org/10.1007/s10812-020-01055-6

Buckley, C. M., Pots, H., Gueho, A., Vines, J. H., Munn, C. J., Phillips, B. A., Gilsbach, B., Traynor, D., Nikolaev, A., Soldati, T., Parnell, A. J., Kortholt, A., & King, J. S. (2020). Coordinated Ras and Rac Activity Shapes Macropinocytic Cups and Enables Phagocytosis of Geometrically Diverse Bacteria. Current Biology, 30(15), 2912-2926.e5. https://doi.org/10.1016/j.cub.2020.05.049

Cankara, F. N., Günaydın, C., Bilge, S. S., Özmen, Ö., & Kortholt, A. (2020). The neuroprotective action of lenalidomide on rotenone model of Parkinson's Disease: Neurotrophic and supportive actions in the substantia nigra pars compacta. Neuroscience Letters, 738, Article 135308. https://doi.org/10.1016/j.neulet.2020.135308

van Haastert, P. J. M. (2020). Symmetry Breaking during Cell Movement in the Context of Excitability, Kinetic Fine-Tuning and Memory of Pseudopod Formation. Cells, 9(8), Article 1809. https://doi.org/10.3390/cells9081809

van Haastert, P. J. M. (2020). Unified control of amoeboid pseudopod extension in multiple organisms by branched F-actin in the front and parallel F-actin/myosin in the cortex. PLoS ONE, 15(12), Article e0243442. https://doi.org/10.1371/journal.pone.0243442

Sabogal-Guáqueta, A. M., Hobbie, F., Keerthi, A., Oun, A., Kortholt, A., Boddeke, E., & Dolga, A. (2019). Linalool attenuates oxidative stress and mitochondrial dysfunction mediated by glutamate and NMDA toxicity. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 118, Article 109295. https://doi.org/10.1016/j.biopha.2019.109295

Wauters, L., Versées, W., & Kortholt, A. (2019). Roco Proteins: GTPases with a Baroque Structure and Mechanism. International Journal of Molecular Sciences, 20(1), Article ijms20010147. https://doi.org/10.3390/ijms20010147

Deyaert, E., Leemans, M., Singh, R. K., Gallardo, R., Steyaert, J., Kortholt, A., Lauer, J., & Versées, W. (2019). Structure and nucleotide-induced conformational dynamics of the Chlorobium tepidum Roco protein. Biochemical Journal, 476(1), 51-66. https://doi.org/10.1042/BCJ20180803

Wauters, L., Terheyden, S., Gilsbach, B. K., Leemans, M., Athanasopoulos, P. S., Guaitoli, G., Wittinghofer, A., Gloeckner, C. J., Versées, W., & Kortholt, A. (2018). Biochemical and kinetic properties of the complex Roco G-protein cycle. Biological Chemistry, 399(12), 1447-1456. https://doi.org/10.1515/hsz-2018-0227

van Haastert, P. J. M., Keizer-Gunnink, I., & Kortholt, A. (2018). The cytoskeleton regulates symmetry transitions in moving amoeboid cells. Journal of Cell Science, 131(7), Article UNSP jcs208892. https://doi.org/10.1242/jcs.208892

Athanasopoulos, P. S., Heumann, R., & Kortholt, A. (2018). The role of (auto)-phosphorylation in the complex activation mechanism of LRRK2. Biological Chemistry, 399(7), 643-647. https://doi.org/10.1515/hsz-2017-0332

Liu, Y., Lacal, J., Firtel, R. A., & Kortholt, A. (2018). Connecting G protein signaling to chemoattractant-mediated cell polarity and cytoskeletal reorganization. Small GTPases, 9(4), 360-364. https://doi.org/10.1080/21541248.2016.1235390

Deyaert, E., Wauters, L., Guaitoli, G., Konijnenberg, A., Leemans, M., Terheyden, S., Petrovic, A., Gallardo, R., Nederveen-Schippers, L. M., Athanasopoulos, P. S., Pots, H., Van Haastert, P. J. M., Sobott, F., Gloeckner, C. J., Efremov, R., Kortholt, A., & Versées, W. (2017). A homologue of the Parkinson's disease-associated protein LRRK2 undergoes a monomer-dimer transition during GTP turnover. Nature Communications, 8(1), 1-12. Article 1008. https://doi.org/10.1038/s41467-017-01103-4

van Haastert, P. J. M., Keizer-Gunnink, I., & Kortholt, A. (2017). Coupled Excitable Ras and F-actin activation mediate spontaneous pseudopod formation and directed cell movement. Molecular Biology of the Cell, 28(7), 922-934. https://doi.org/10.1091/mbc.E16-10-0733

Xu, X., Wen, X., Veltman, D. M., Keizer-Gunnink, I., Pots, H., Kortholt, A., & Jin, T. (2017). GPCR-controlled membrane recruitment of negative regulator C2GAP1 locally inhibits Ras signaling for adaptation and long-range chemotaxis. Proceedings of the National Academy of Sciences of the United States of America, 114(47), 10092-10101. https://doi.org/10.1073/pnas.1703208114

Hilbi, H., & Kortholt, A. (2017). Role of the small GTPase Rap1 in signal transduction, cell dynamics and bacterial infection. Small GTPases, 10(5), 336-342. https://doi.org/10.1080/21541248.2017.1331721

Deyaert, E., Kortholt, A., & Versées, W. (2017). The LRR-Roc-COR module of the Chlorobium tepidum Roco protein: crystallization and X-ray crystallographic analysis. Acta Crystallographica Section F: Structural Biology Communications , 73(9), 520-524. https://doi.org/10.1107/S2053230X17011955

Rosenbusch, K. E., & Kortholt, A. (2016). Activation Mechanism of LRRK2 and Its Cellular Functions in Parkinson's Disease. Parkinsons disease, 2016, Article ID 7351985. https://doi.org/10.1155/2016/7351985

Liu, Y., Lacal, J., Veltman, D. M., Fusetti, F., van Haastert, P. J. M., Firtel, R. A., & Kortholt, A. (2016). A Gα-Stimulated RapGEF Is a Receptor-Proximal Regulator of Dictyostelium Chemotaxis. Developmental Cell, 37(5), 458-472. https://doi.org/10.1016/j.devcel.2016.05.001

Skoge, M., Wong, E., Hamza, B., Bae, A., Martel, J., Kataria, R., Keizer-Gunnink, I., Kortholt, A., Van Haastert, P. J. M., Charras, G., Janetopoulos, C., & Irimia, D. (2016). A Worldwide Competition to Compare the Speed and Chemotactic Accuracy of Neutrophil-Like Cells. PLoS ONE, 11(6), Article e0154491. https://doi.org/10.1371/journal.pone.0154491

Plak, K., Pots, H., Van Haastert, P. J. M., & Kortholt, A. (2016). Direct Interaction between TalinB and Rap1 is necessary for adhesion of Dictyostelium cells. BMC Cell Biology, 17(1), 1-8. https://doi.org/10.1186/s12860-015-0078-0

Kamp, M. E., Liu, Y., & Kortholt, A. (2016). Function and Regulation of Heterotrimeric G Proteins during Chemotaxis. International Journal of Molecular Sciences, 17(1), Article 90. https://doi.org/10.3390/ijms17010090

Guaitoli, G., Raimondi, F., Gilsbach, B. K., Gómez-Llorente, Y., Deyaert, E., Renzi, F., Li, X., Schaffner, A., Jagtap, P. K. A., Boldt, K., von Zweydorf, F., Gotthardt, K., Lorimer, D. D., Yue, Z., Burgin, A., Janjic, N., Sattler, M., Versées, W., Ueffing, M., ... Gloeckner, C. J. (2016). Structural model of the dimeric Parkinson's protein LRRK2 reveals a compact architecture involving distant interdomain contacts. Proceedings of the National Academy of Sciences of the United States of America, 113(30), E4357-E4366. https://doi.org/10.1073/pnas.1523708113

Khanna, A., Lotfi, P., Chavan, A. J., Montaño, N. M., Bolourani, P., Weeks, G., Shen, Z., Briggs, S. P., Pots, H., Van Haastert, P. J. M., Kortholt, A., & Charest, P. G. (2016). The small GTPases Ras and Rap1 bind to and control TORC2 activity. Scientific Reports, 6, 1-9. Article 25823. https://doi.org/10.1038/srep25823

Terheyden, S., Nederveen-Schippers, L. M., & Kortholt, A. (2016). The unconventional G-protein cycle of LRRK2 and Roco proteins. Biochemical Society Transactions, 44(6), 1611-1616. https://doi.org/10.1042/BST20160224

Postma, M., & van Haastert, P. J. M. (2016). Mathematics of Experimentally Generated Chemoattractant Gradients. Methods in Molecular Biology, 1407, 381-396. https://doi.org/10.1007/978-1-4939-3480-5_26

Rudi, K., Ho, F. Y., Gilsbach, B. K., Pots, H., Wittinghofer, A., Kortholt, A., & Klare, J. P. (2015). Conformational heterogeneity of the Roc domains in C. tepidum Roc-COR and implications for human LRRK2 Parkinson mutations. Bioscience reports, 35(5), 1-12. Article e00254. https://doi.org/10.1042/BSR20150128

Wu, J., Pipathsouk, A., Keizer-Gunnink, A., Fusetti, F., Alkema, W., Liu, S., Altschuler, S., Wu, L., Kortholt, A., & Weiner, O. D. (2015). Homer3 regulates the establishment of neutrophil polarity. Molecular Biology of the Cell, 26(9), 1629-1639. https://doi.org/10.1091/mbc.E14-07-1197

Terheyden, S., Ho, F. Y., Gilsbach, B. K., Wittinghofer, A., & Kortholt, A. (2015). Revisiting the Roco G-protein cycle. Biochemical Journal, 465(1), 139-147. https://doi.org/10.1042/BJ20141095

Gilsbach, B. K., Messias, A. C., Ito, G., Sattler, M., Alessi, D. R., Wittinghofer, A., & Kortholt, A. (2015). Structural Characterization of LRRK2 Inhibitors. Journal of Medicinal Chemistry, 58(9), 3751−3756. https://doi.org/10.1021/jm5018779

Plak, K., Keizer-Gunnink, I., van Haastert, P. J. M., & Kortholt, A. (2014). Rap1-dependent pathways coordinate cytokinesis in Dictyostelium. Molecular Biology of the Cell, 25(25), 4195-4204. https://doi.org/10.1091/mbc.E14-08-1285

Gilsbach, B. K., & Kortholt, A. (2014). Structural biology of the LRRK2 GTPase and kinase domains: implications for regulation. Frontiers in Molecular Neuroscience, 7, Article 32. https://doi.org/10.3389/fnmol.2014.00032

Koelsch, V., Shen, Z., Lee, S., Plak, K., Lotfi, P., Chang, J., Charest, P. G., Romero, J. L., Jeon, T. J., Kortholt, A., Briggs, S. P., & Firtel, R. A. (2013). Daydreamer, a Ras effector and GSK-3 substrate, is important for directional sensing and cell motility. Molecular Biology of the Cell, 24(2), 100-114. https://doi.org/10.1091/mbc.E12-04-0271

Kataria, R., Xu, X., Fusetti, F., Keizer-Gunnink, I., Jin, T., van Haastert, P. J. M., & Kortholt, A. (2013). Dictyostelium Ric8 is a nonreceptor guanine exchange factor for heterotrimeric G proteins and is important for development and chemotaxis. Proceedings of the National Academy of Sciences of the United States of America, 110(16), 6424-6429. https://doi.org/10.1073/pnas.1301851110

Plak, K., Veltman, D., Fusetti, F., Beeksma, J., Rivero, F., Van Haastert, P. J. M., & Kortholt, A. (2013). GxcC connects Rap and Rac signaling during Dictyostelium development. BMC Cell Biology, 14(1), 6-1-6-9. Article 6. https://doi.org/10.1186/1471-2121-14-6

Kortholt, A., Keizer-Gunnink, I., Kataria, R., & Van Haastert, P. J. M. (2013). Ras activation and symmetry breaking during Dictyostelium chemotaxis. Journal of Cell Science, 126(19), 4502-4513. https://doi.org/10.1242/jcs.132340

Kataria, R., van Haastert, P. J. M., & Kortholt, A. (2013). Reply to Tall et al. Dictyostelium Ric8 does not have a chaperoning function during development and chemotaxis. Proceedings of the National Academy of Sciences of the United States of America, 110(34), E3149-E3149. https://doi.org/10.1073/pnas.1309837110

Mueller-Taubenberger, A., Kortholt, A., & Eichinger, L. (2013). Simple system - substantial share: The use of Dictyosrelium in cell biology and molecular medicine. European Journal of Cell Biology, 92(2), 45-53. https://doi.org/10.1016/j.ejcb.2012.10.003

Veltman, D. M., & Van Haastert, P. J. M. (2013). Extrachromosomal inducible expression. Methods in Molecular Biology, 983, 269-281. https://doi.org/10.1007/978-1-62703-302-2_14

Kortholt, A., van Egmond, W. N., Plak, K., Bosgraaf, L., Keizer-Gunnink, I., & van Haastert, P. J. M. (2012). Multiple Regulatory Mechanisms for the Dictyostelium Roco Protein GbpC. The Journal of Biological Chemistry, 287(4), 2749-2758. https://doi.org/10.1074/jbc.M111.315739

Gilsbach, B. K., Ho, F. Y., Vetter, I. R., van Haastert, P. J. M., Wittinghofer, A., & Kortholt, A. (2012). Roco kinase structures give insights into the mechanism of Parkinson disease-related leucine-rich-repeat kinase 2 mutations. Proceedings of the National Academy of Sciences of the United States of America, 109(26), 10322-10327. https://doi.org/10.1073/pnas.1203223109

Kortholt, A., Kataria, R., Keizer-Gunnink, I., Van Egmond, W. N., Khanna, A., & Van Haastert, P. J. M. (2011). Dictyostelium chemotaxis: essential Ras activation and accessory signalling pathways for amplification. Embo Reports, 12(12), 1273-1279. https://doi.org/10.1038/embor.2011.210

Van Haastert, P. J. M. (2011). Amoeboid Cells Use Protrusions for Walking, Gliding and Swimming. PLoS ONE, 6(11), Article e27532. https://doi.org/10.1371/journal.pone.0027532

Neilson, M. P., Veltman, D. M., van Haastert, P. J. M., Webb, S. D., Mackenzie, J. A., & Insall, R. H. (2011). Chemotaxis: A Feedback-Based Computational Model Robustly Predicts Multiple Aspects of Real Cell Behaviour. PLOS BIOLOGY, 9(5), Article 1000618. https://doi.org/10.1371/journal.pbio.1000618

Van Haastert, P. J. M. (2011). How Cells Use Pseudopods for Persistent Movement and Navigation. Science signaling, 4(159), Article 6. https://doi.org/10.1126/scisignal.2001708

Kortholt, A., Bolourani, P., Rehmann, H., Keizer-Gunnink, I., Weeks, G., Wittinghofer, A., & Van Haastert, P. J. M. (2010). A Rap/Phosphatidylinositol 3-Kinase Pathway ControlsPseudopod Formation. Molecular Biology of the Cell, 21(6), 936-945. https://doi.org/10.1091/mbc.E09-03-0177

Van Haastert, P. J. M. (2010). A Model for a Correlated Random Walk Based on the Ordered Extension of Pseudopodia. PLoS Computational Biology, 6(8), Article e1000874. https://doi.org/10.1371/journal.pcbi.1000874

Van Haastert, P. J. M. (2010). A Stochastic Model for Chemotaxis Based on the Ordered Extension of Pseudopods. Biophysical Journal, 99(10), 3345-3354. https://doi.org/10.1016/j.bpj.2010.09.042

van Egmond, W. N., & van Haastert, P. J. M. (2010). Characterization of the Roco Protein Family in Dictyostelium discoideum. Eukaryotic Cell, 9(5), 751-761. https://doi.org/10.1128/EC.00366-09

Van Haastert, P. J. M. (2010). Chemotaxis: insights from the extending pseudopod. Journal of Cell Science, 123(18), 3031-3037. https://doi.org/10.1242/jcs.071118

Araki, T., van Egmond, W. N., van Haastert, P. J. M., & Williams, J. G. (2010). Dual regulation of a Dictyostelium STAT by cGMP and Ca2+ signalling. Journal of Cell Science, 123(6), 837-841. https://doi.org/10.1242/jcs.064436

Bosgraaf, L., & Van Haastert, P. J. M. (2010). Quimp3, an automated pseudopod-tracking algorithm. Cell Adhesion & Migration, 4(1), 46-55. https://doi.org/10.4161/cam.4.1.9953

Bosgraaf, L., van Haastert, P. J. M., & Bretschneider, T. (2009). Analysis of Cell Movement by Simultaneous Quantification of Local Membrane Displacement and Fluorescent Intensities Using Quimp2. Cell Motility and the Cytoskeleton, 66(3), 156-165. https://doi.org/10.1002/cm.20338

Veltman, D. M., Akar, G., Bosgraaf, L., & Van Haastert, P. J. M. (2009). A new set of small, extrachromosomal expression vectors for Dictyostelium discoideum. Plasmid, 61(2), 110-118. https://doi.org/10.1016/j.plasmid.2008.11.003

Veltman, D. M., Keizer-Gunnink, I., & Van Haastert, P. J. M. (2009). An extrachromosomal, inducible expression system for Dictyostelium discoideum. Plasmid, 61(2), 119-125. https://doi.org/10.1016/j.plasmid.2008.11.002

Van Haastert, P. J. M., & Bosgraaf, L. (2009). Food Searching Strategy of Amoeboid Cells by Starvation Induced Run Length Extension. PLoS ONE, 4(8), Article 6814. https://doi.org/10.1371/journal.pone.0006814

Bosgraaf, L., & Van Haastert, P. J. M. (2009). Navigation of Chemotactic Cells by Parallel Signaling to Pseudopod Persistence and Orientation. PLoS ONE, 4(8), Article 6842. https://doi.org/10.1371/journal.pone.0006842

Sato, M. J., Kuwayama, H., van Egmond, W. N., Takayama, A. L. K., Takagi, H., van Haastert, P. J. M., Yanagida, T., & Ueda, M. (2009). Switching direction in electric-signal-induced cell migration by cyclic guanosine monophosphate and phosphatidylinositol signaling. Proceedings of the National Academy of Sciences of the United States of America, 106(16), 6667-6672. https://doi.org/10.1073/pnas.0809974106

Van Haastert, P. J. M., & Bosgraaf, L. (2009). The local cell curvature guides pseudopodia towards chemoattractants. HFSP Journal, 3(4), 282-286. https://doi.org/10.2976/1.3185725

Bosgraaf, L., & Van Haastert, P. J. M. (2009). The Ordered Extension of Pseudopodia by Amoeboid Cells in the Absence of External Cues. PLoS ONE, 4(4), Article 5253. https://doi.org/10.1371/journal.pone.0005253

Kortholt, A., & van Haastert, P. J. M. (2008). Highlighting the role of Ras and Rap during Dictyostelium chemotaxis. Cellular Signalling, 20(8), 1415-1422. https://doi.org/10.1016/j.cellsig.2008.02.006

Egmond, W. N. V., Kortholt, A., Plak, K., Bosgraaf, L., Bosgraaf, S., Keizer-Gunnink, I., & Haastert, P. J. M. V. (2008). Intramolecular Activation Mechanism of the Dictyostelium LRRK2 Homolog Roco Protein GbpC. The Journal of Biological Chemistry, 283(44). https://doi.org/10.1074/jbc.M804265200

Gotthardt, K., Weyand, M., Kortholt, A., Van Haastert, P. J. M., & Wittinghofer, A. (2008). Structure of the Roc-COR domain tandem of C-tepidum, a prokaryotic homologue of the human LRRK2 Parkinson kinase. EMBO Journal, 27(16), 2239-2249. https://doi.org/10.1038/emboj.2008.150

Veltman, D. M., Keizer-Gunnink, I., & Van Haastert, P. J. M. (2008). Four key signaling pathways mediating chemotaxis in Dictyostelium discoideum. Journal of Cell Biology, 180(4), 747-753. https://doi.org/10.1083/jcb.200709180

Bosgraaf, L., Keizer-Gunnink, I., & van Haastert, P. J. M. (2008). PI3-kinase signaling contributes to orientation in shallow gradients and enhances speed in steep chemoattractant gradients. Journal of Cell Science, 121(21), 3589-3597. https://doi.org/10.1242/jcs.031781

Marin, I., van Egmond, W. N., & van Haastert, P. J. M. (2008). The Roco protein family: a functional perspective. The FASEB Journal, 22(9), 3103-3110. https://doi.org/10.1096/fj.08-111310

Veltman, D. M., & van Haastert, P. J. M. (2008). The role of cGMP and the rear of the cell in Dictyostelium chemotaxis and cell streaming. Journal of Cell Science, 121(1), 120-127. https://doi.org/10.1242/jcs.015602

Keizer-Gunnink, I., Kortholt, A., & Van Haastert, P. J. M. (2007). Chemoattractants and chemorepellents act by inducing opposite polarity in phospholipase C and PI3-kinase signaling. Journal of Cell Biology, 177(4), 579-585. https://doi.org/10.1083/jcb.200611046

Kae, H., Kortholt, A., Rehmann, H., Insall, R., Van Haastert, P. J. M., Spiegelman, G. B., & Weeks, G. (2007). Cyclic AMP signalling in Dictyostelium: G-proteins activate separate Ras pathways using specific RasGEFs. Embo Reports, 8(5), 477-482. https://doi.org/10.1038/sj.embor.7400936

van Haastert, P. J. M., Keizer-Gunnink, I., & Kortholt, A. (2007). Essential role of PI3-kinase and phospholipase A2 in Dictyostelium discoideum chemotaxis. Journal of Cell Biology, 177(5), 809-816. https://doi.org/10.1083/jcb.200701134

Kortholt, A., King, J. S., Keizer-Gunnink, I., Harwood, A. J., & Van Haastert, P. J. M. (2007). Phospholipase C regulation of phosphatidylinositol 3,4,5-trisphosphate-mediated chemotaxis. Molecular Biology of the Cell, 18(12), 4772-4779. https://doi.org/10.1091/mbc.E07-05-0407

Loovers, H. M., Kortholt, A., de Groote, H., Whitty, L., Nussbaum, R. L., & van Haastert, P. J. M. (2007). Regulation of phagocytosis in Dictyostelium by the inositol 5-phosphatase OCRL homolog Dd5P4. Traffic, 8(5), 618 - 628. https://doi.org/10.1111/j.1600-0854.2007.00546.x

Bader, S., Kortholt, A., & van Haastert, P. J. M. (2007). Seven Dictyostelium discoideum phosphodiesterases degrade three pools of cAMP and cGMP. Biochemical Journal, 402(1), 153 - 161. https://doi.org/10.1042/BJ20061153

van Haastert, P. J. M., & Postma, M. (2007). Biased random walk by Stochastic fluctuations of chemoattractant-receptor interactions at the lower limit of detection. Biophysical Journal, 93(5), 1787-1796. https://doi.org/10.1529/biophysj.107.104356

Kortholt, A., Rehmann, H., Kae, H., Bosgraaf, L., Keizer-Gunnink, I., Weeks, G., Wittinghofer, A., & Haastert, P. J. M. V. (2006). Characterization of the GbpD-activated Rap1 Pathway Regulating Adhesion and Cell Polarity in Dictyostelium discoideum. The Journal of Biological Chemistry, 281(33), 23367 - 23376. https://doi.org/10.1074/jbc.M600804200

Bader, S., Kortholt, A., Snippe, H., & van Haastert, P. J. M. (2006). DdPDE4, a novel cAMP-specific phosphodiesterase at the surface of dictyostelium cells. The Journal of Biological Chemistry, 281(29), 20018 - 20026. https://doi.org/10.1074/jbc.M600040200

Loovers, H., Postma, M., Keizer-Gunnink, I., Huang, Y. E., Devreotes, P. N., & Haastert, P. J. M. V. (2006). Distinct Roles of PI(3,4,5)P3 during Chemoattractant Signaling in Dictyostelium: A Quantitative In Vivo Analysis by Inhibition of PI3-Kinase. Molecular Biology of the Cell, 17(4), 1503-1513. https://doi.org/10.1091/mbc.E05-09-0825

Veltman, D. M., & van Haastert, P. J. M. (2006). Guanylyl cyclase protein and cGMP product independently control front and back of chemotaxing Dictyostelium cells. Molecular Biology of the Cell, 17(9), 3921 - 3929. https://doi.org/10.1091/mbc.E06-05-0381

Goldberg, J. M., Wolpin, E. S., Bosgraaf, L., Clarkson, B. K., van Haastert, P. J. M., & Smith, J. L. (2006). Myosin light chain kinase A is activated by cGMP-dependent and cGMP-independent pathways. FEBS Letters, 580(8), 2059 - 2064. https://doi.org/10.1016/j.febslet.2006.03.008

Engel, R., van Haastert, P. J. M., & Visser, A. J. W. G. (2006). Spectral characterization of Dictyostelium autofluorescence. Microscopy Research and Technique, 69(3), 168-174. https://doi.org/10.1002/jemt.20282

Bosgraaf, L., & van Haastert, P. J. M. (2006). The regulation of myosin II in Dictyostelium. European Journal of Cell Biology, 85(9-10), 969 - 979. https://doi.org/10.1016/j.ejcb.2006.04.004

Veltman, D. M., Roelofs, J., Engel, R., Visser, A. J. W. G., & Haastert, P. J. M. V. (2005). Activation of soluble guanylyl cyclase at the leading edge during Dictyostelium chemotaxis. Molecular Biology of the Cell, 16(2), 976 - 983. https://doi.org/10.1091/mbc.E04-08-0701

Bukharova, T., Weijer, G., Bosgraaf, L., Dormann, D., van Haastert, P. J., & Weijer, C. J. (2005). Paxillin is required for cell-substrate adhesion, cell sorting and slug migration during Dictyostelium development (vol 118, pg 4295, 2005). Journal of Cell Science, 118(18), 4295 - 4310. https://doi.org/10.1242/jcs.02557

Bosgraaf, L., Waijer, A., Engel, R., Visser, A. J. W. G., Wessels, D., Soll, D., & van Haastert, P. J. M. (2005). RasGEF-containing proteins GbpC and GbpD have differential effects on cell polarity and chemotaxis in Dictyostelium. Journal of Cell Science, 118(9), 1899-1910. https://doi.org/10.1242/jcs.02317

Knol, J., Engel, R., Blaauw, M., Visser, A. J. W. G., & Haastert, P. J. M. V. (2005). The Phosducin-Like Protein PhLP1 Is Essential for Gβγ Dimer Formation in Dictyostelium discoideum. Molecular and Cellular Biology, 25(18), 8393-8400. https://doi.org/10.1128/MCB.25.18.8393-8400.2005

Haastert, P. J. M. V., & Devreotes, P. N. (2004). Chemotaxis: signalling modules join hands at front and tail. Nature Reviews Molecular Cell Biology, 5(8), 626 - 634. https://doi.org/10.1038/sj.embor.7400051

Lu, Y. H., Knol, J. C., Linskens, M. H. K., Friehs, K., van Haastert, P. J. M., & Flaschel, E. (2004). Cultivation of immobilized Dictyostelium discoideum for the production of soluble human Fas ligand. Applied Microbiology and Biotechnology, 65(5), 547 - 552. https://doi.org/10.1007/s00253-004-1620-8

Verheijden, G. F. M., Metz, B., van Ravestein, A. A. M., Bos, E. S., van de Wetering, R., Westerveld, A., Kouwijzer, M., Hanssen, R. G. J. M., van Peij, N. V., van Haastert, P. J. M., Linskens, M. H. K., & van Peij, NN. (2004). Human gonadotropins expressed in dictyostelium discoideum. Biology of Reproduction, 177 - 177.

Engel, R., Hink, M. A., Bosgraaf, L., Haastert, P. J. M. V., & Visser, A. J. W. G. (2004). Pleckstrin Homology Domain Diffusion in Dictyostelium Cytoplasm Studied Using Fluorescence Correlation Spectroscopy. The Journal of Biological Chemistry, 279(11), 10013-10019. https://doi.org/10.1074/jbc.M310039200

Lu, Y. H., Knol, J. C., Linskens, M. H. K., Friehs, K., van Haastert, P. J. M., & Flaschel, E. (2004). Production of the soluble human Fas ligand by Dictyostelium discoideum cultivated on a synthetic medium. Journal of Biotechnology, 108(3), 243-251. https://doi.org/10.1016/j.jbiotec.2003.12.006

Postma, M., Roelofs, J., Goedhart, J., Loovers, H. M., Visser, A. J. W. G., & van Haastert, P. J. M. (2004). Sensitization of Dictyostelium chemotaxis by phosphoinositide-3-kinase-mediated self-organizing signalling patches. Journal of Cell Science, 117(14), 2925-2935. https://doi.org/10.1242/jcs.01143

Veltman, D. M., Bosgraaf, L., & van Haastert, P. J. M. (2004). Unusual guanylyl cyclases and cGMP signaling in Dictyostelium discoideum. Vitamins and Hormones - Advances in Research and Applications, 69, 95-115.

Blaauw, M., Knol, JC., Kortholt, A., Roelofs, J., (Engel), R., Postma, M., Visser, AJWG., Van Haastert, PJM., Knol, J. C., & Visser, A. J. W. G. (2003). Phosducin-like proteins in Dictyostelium discoideum: implications for the phosducin family of proteins. EMBO Journal, 22(19), 5047-5057. https://doi.org/10.1093/emboj/cdg508

Loovers, HM., Veenstra, K., Snippe, H., Pesesse, N. V., Erneux, C., van Haastert, PJM., Loovers, H. M., & Pesesse, X. (2003). A diverse family of inositol 5-phosphatases playing a role in growth and development in Dictyostelium discoideum. The Journal of Biological Chemistry, 278(8), 5652-5658. https://doi.org/10.1074/jbc.M208396200

Roelofs, J., Smith, J. L., & Haastert, P. J. M. V. (2003). cGMP signalling: different ways to create a pathway. Trends in Genetics, 19(3), 132-134. https://doi.org/10.1016/S0168-9525(02)00044-6

Veltman, DM., De Boer, JS., Van Haastert, PJM., & Boer, J. S. D. (2003). Chemoattractant-stimulated calcium influx in Dictyostelium discoideum does not depend on cGMP. Biochimica et Biophysica Acta-General Subjects, 1623(2-3), 129-134. https://doi.org/10.1016/j.bbagen.2003.08.006

Veltman, D., & Van Haastert, PJM. (2003). Regulation of Dictyostelium guanylyl cyclases. Protist, 154(1), 33-42. https://doi.org/10.1078/143446103764928477

Bosgraaf, L., & Haastert, P. J. M. V. (2003). Roc, a Ras/GTPase domain in complex proteins. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1643(1), 5 - 10. https://doi.org/10.1016/j.bbamcr.2003.08.008

Postma, M., Roelofs, J., Goedhart, J., Gadella, TWJ., Visser, AJWG., Van Haastert, PJM., Gadella, T. W. J., & Visser, A. J. W. G. (2003). Uniform cAMP stimulation of Dictyostelium cells induces localized patches of signal transduction and pseudopodia. Molecular Biology of the Cell, 14(12), 5019-5027. https://doi.org/10.1091/mbc.E03-08-0566

Bosgraaf, L., & Haastert, P. J. M. V. (2002). A model for cGMP signal transduction in Dictyostelium in perspective of 25 years of cGMP research. Journal of muscle research and cell motility, 23(7-8), 781-791.

Bosgraaf, L., Russcher, H., Smith, J. L., Wessels, D., Soll, D. R., & Haastert, P. J. M. V. (2002). A novel cGMP signalling pathway mediating myosin phosphorylation and chemotaxis in Dictyostelium. The EMBO Journal, 21(17), 4560-4570. https://doi.org/10.1093/emboj/cdf438

Roelofs, J., & Haastert, P. J. M. V. (2002). Characterization of two unusual guanylyl cyclases from Dictyostelium. The Journal of Biological Chemistry, 277(11), 9167-9174. https://doi.org/10.1074/jbc.M111437200

Roelofs, J., & Haastert, P. J. M. V. (2002). Deducing the origin of soluble adenylyl cyclase, a gene lost in multiple lineages. Molecular Biology and Evolution, 19(12), 2239-2246. https://doi.org/10.1093/oxfordjournals.molbev.a004047

Bosgraaf, L., Russcher, H., Snippe, H., Bader, S., Wind, J., & Van Haastert, PJM. (2002). Identification and characterization of two unusual cGMP-stimulated phoshodiesterases in Dictyostelium. Molecular Biology of the Cell, 13(11), 3878-3889. https://doi.org/10.1091/mbc.E02-05-0302

Goldberg, JM., Bosgraaf, L., Van Haastert, PJM., & Smith, JL. (2002). Identification of four candidate cGMP targets in Dictylostelium. Proceedings of the National Academy of Sciences of the United States of America, 99(10), 6749-6754. https://doi.org/10.1073/pnas.102167299

Postma, M., & Van Haastert, PJM. (2001). A diffusion-translocation model for gradient sensing by chemotactic cells. Biophysical Journal, 81(3), 1314-1323. https://doi.org/10.1016/S0006-3495(01)75788-8

Roelofs, J., & Van Haastert, PJM. (2001). Genomics: Genes lost during evolution. Nature, 411(6841), 1013-1014. https://doi.org/10.1038/35082627

Roelofs, J., Loovers, H., & Van Haastert, PJM. (2001). GTPγS Regulation of a 12-Transmembrane Guanylyl Cyclase Is Retained after Mutation to an Adenylyl Cyclase. The Journal of Biological Chemistry, 276(44), 40740-40745. https://doi.org/10.1074/jbc.M105154200

Roelofs, J., Snippe, H., Kleineidam, RG., & Van Haastert, PJM. (2001). Guanylate cyclase in Dictyostelium discoideum with the topology of mammalian adenylate cyclase. Biochemical Journal, 354(3), 697-706. https://doi.org/10.1042/0264-6021:3540697

Kuwayama, H., Snippe, H., Derks, M., Roelofs, J., & van Haastert, PJM. (2001). Identification and characterization of DdPDE3, a cGMP-selective phosphodiesterase from Dictyostelium. Biochemical Journal, 353(3), 635-644. https://doi.org/10.1042/0264-6021:3530635

Dijken, P. V., & Haastert, P. J. M. V. (2001). Phospholipase Cδ regulates germination of Dictyostelium spores. BMC Cell Biology, 2, Article 25. https://doi.org/10.1186/1471-2121-2-25

Potma, E. O., Boeij, W. P. D., Haastert, P. J. M. V., & Wiersma, D. A. (2001). Real-time visualization of intracellular hydrodynamics in single living cells. Proceedings of the National Academy of Sciences, 98(4), 1577 - 1582. https://doi.org/10.1073/pnas.98.4.1577

Potma, E. O., de Boeij, W. P., Bosgraaf, L., Roelofs, J., van Haastert, P. J. M., & Wiersma, D. A. (2001). Reduced protein diffusion rate by cytoskeleton in vegetative and polarized Dictyostelium cells. Biophysical Journal, 81(4), 2010 - 2019. https://doi.org/10.1016/S0006-3495(01)75851-1

Roelofs, J., Meima, M., Schaap, P., & Van Haastert, PJM. (2001). The Dictyostelium homologue of mammalian soluble adenylyl cyclase encodes a guanylyl cyclase. EMBO Journal, 20(16), 4341-4348. https://doi.org/10.1093/emboj/20.16.4341

Blaauw, M., Linskens, MHK., & van Haastert, PJM. (2000). Efficient control of gene expression by a tetracycline-dependent transactivator in single Dictyostelium discoideum cells. Gene, 252(1-2), 71-82. https://doi.org/10.1016/S0378-1119(00)00227-4

Vervoort, E. B., Ravestein, A. V., Peij , van, N., Heikoop, J. C., Haastert, van, P. J. M., Verheijden, G. F., & Linskens, M. H. K. (2000). Optimizing heterologous expression in Dictyostelium: importance of 5 ' codon adaptation. Nucleic Acids Research, 28(10), 2069-2074. https://doi.org/10.1093/nar/28.10.2069