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Research GBB Cell Biochemistry Publications

Publications

2021

Rosenbusch, I., Oun, A., Sanislav, O., Lay, S. T., Keizer-Gunnink, I., Annesley, S. J., Fisher, P. R., Dolga, A., & Kortholt, A. (2021). A conserved role for LRRK2 and Roco proteins in the regulation of mitochondrial activity. Frontiers in Cell and Developmental Biology, 9, [734554].
Helton, L. G., Soliman, A., von Zweydorf, F., Kentros, M., Manschwetus, J. T., Hall, S., Gilsbach, B., Ho, F. Y., Athanasopoulos, P. S., Singh, R. K., LeClair, T. J., Versées, W., Raimondi, F., Herberg, F. W., Gloeckner, C. J., Rideout, H., Kortholt, A., & Kennedy, E. J. (2021). Allosteric Inhibition of Parkinson's-Linked LRRK2 by Constrained Peptides. ACS chemical biology, [acschembio.1c00487]. https://doi.org/10.1021/acschembio.1c00487
Kennedy, E., Kortholt, A., & Helton, L. G. (2021). Chemically-stabilized allosteric modulators of leucine-rich repeat kinase 2 (lrrk2). (Patent No. WO2021173802).
Nederveen-Schippers, L. M., Pathak, P., Keizer-Gunnink, I., Westphal, A. H., van Haastert, P. J. M., Borst, J. W., Kortholt, A., & Skakun, V. (2021). Combined FCS and PCH analysis to quantify protein dimerization in living cells. International Journal of Molecular Sciences, 22(14), [7300]. https://doi.org/10.3390/ijms22147300
Wojewska, D. N., & Kortholt, A. (2021). Lrrk2 targeting strategies as potential treatment of parkinson’s disease. Biomolecules, 11(8), [1101]. https://doi.org/10.3390/biom11081101
Xu, X., Bhimani, S., Pots, H., Wen, X., Jeon, T. J., Kortholt, A., & Jin, T. (2021). Membrane Targeting of C2GAP1 Enables Dictyostelium discoideum to Sense Chemoattractant Gradient at a Higher Concentration Range. Frontiers in Cell and Developmental Biology, 9, [725073]. https://doi.org/10.3389/fcell.2021.725073
van Haastert, P. J. M., Keizer-Gunnink, I., Pots, H., Ortiz-Mateos, C., Veltman, D., van Egmond, W., & Kortholt, A. (2021). 45 years of cGMP research in Dictyostelium: Understanding the regulation and function of the cGMP pathway for cell movement and chemotaxis. Molecular Biology of the Cell, 32(20), [mbcE21040171]. https://doi.org/10.1091/mbc.E21-04-0171
van Haastert, P. J. M. (2021). Short- and long-term memory of moving amoeboid cells. PLoS ONE, 16(2), [e0246345]. https://doi.org/10.1371/journal.pone.0246345

2020

Konstantinidou, M., Oun, A., Pathak, P., Zhang, B., Wang, Z., ter Brake, F., Dolga, A., Kortholt, A., & Doemling, A. (2021). The tale of proteolysis targeting chimeras (PROTACs) for leucine-rich repeat kinase 2 (LRRK2). ChemMedChem, 16(6), 959-965. https://doi.org/10.1002/cmdc.202000872
Soliman, A., Cankara, F. N., & Kortholt, A. (2020). Allosteric inhibition of LRRK2, where are we now. Biochemical Society Transactions, 48(5), 2185-2194. [BST20200424]. https://doi.org/10.1042/BST20200424
Leemans, M., Galicia, C., Deyaert, E., Daems, E., Krause, L., Paesmans, J., Pardon, E., Steyaert, J., Kortholt, A., Sobott, F., Klostermeier, D., & Versées, W. (2020). Allosteric modulation of the GTPase activity of a bacterial LRRK2 homolog by conformation-specific Nanobodies. Biochemical Journal, 477(7), 1203-1218. https://doi.org/10.1042/BCJ20190843
Yatskou, M. M., Skakun, V. V., Nederveen-Schippers, L., Kortholt, A., & Apanasovich, V. V. (2020). Complex Analysis of Fluorescence Intensity Fluctuations of Molecular Compounds. Journal of applied spectroscopy, 87(4), 685-692. https://doi.org/10.1007/s10812-020-01055-6
Buckley, C. M., Pots, H., Gueho, A., Vines, J. H., Munn, C. J., Phillips, B. A., Gilsbach, B., Traynor, D., Nikolaev, A., Soldati, T., Parnell, A. J., Kortholt, A., & King, J. S. (2020). Coordinated Ras and Rac Activity Shapes Macropinocytic Cups and Enables Phagocytosis of Geometrically Diverse Bacteria. Current Biology, 30(15), 2912-2926.e5. https://doi.org/10.1016/j.cub.2020.05.049
Cankara, F. N., Günaydın, C., Bilge, S. S., Özmen, Ö., & Kortholt, A. (2020). The neuroprotective action of lenalidomide on rotenone model of Parkinson's Disease: Neurotrophic and supportive actions in the substantia nigra pars compacta. Neuroscience Letters, 738, [135308]. https://doi.org/10.1016/j.neulet.2020.135308
van Haastert, P. J. M. (2020). Symmetry Breaking during Cell Movement in the Context of Excitability, Kinetic Fine-Tuning and Memory of Pseudopod Formation. Cells, 9(8), [1809]. https://doi.org/10.3390/cells9081809
van Haastert, P. J. M. (2020). Unified control of amoeboid pseudopod extension in multiple organisms by branched F-actin in the front and parallel F-actin/myosin in the cortex. PLoS ONE, 15(12), [e0243442]. https://doi.org/10.1371/journal.pone.0243442

2019

Sabogal-Guáqueta, A. M., Hobbie, F., Keerthi, A., Oun, A., Kortholt, A., Boddeke, E., & Dolga, A. (2019). Linalool attenuates oxidative stress and mitochondrial dysfunction mediated by glutamate and NMDA toxicity. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 118, [109295]. https://doi.org/10.1016/j.biopha.2019.109295
Wauters, L., Versées, W., & Kortholt, A. (2019). Roco Proteins: GTPases with a Baroque Structure and Mechanism. International Journal of Molecular Sciences, 20(1), [ijms20010147]. https://doi.org/10.3390/ijms20010147

2018

Deyaert, E., Leemans, M., Singh, R. K., Gallardo, R., Steyaert, J., Kortholt, A., Lauer, J., & Versées, W. (2019). Structure and nucleotide-induced conformational dynamics of the Chlorobium tepidum Roco protein. Biochemical Journal, 476(1), 51-66. https://doi.org/10.1042/BCJ20180803
Wauters, L., Terheyden, S., Gilsbach, B. K., Leemans, M., Athanasopoulos, P. S., Guaitoli, G., Wittinghofer, A., Gloeckner, C. J., Versées, W., & Kortholt, A. (2018). Biochemical and kinetic properties of the complex Roco G-protein cycle. Biological Chemistry, 399(12), 1447-1456. https://doi.org/10.1515/hsz-2018-0227
van Haastert, P. J. M., Keizer-Gunnink, I., & Kortholt, A. (2018). The cytoskeleton regulates symmetry transitions in moving amoeboid cells. Journal of Cell Science, 131(7), [UNSP jcs208892]. https://doi.org/10.1242/jcs.208892
Athanasopoulos, P. S., Heumann, R., & Kortholt, A. (2018). The role of (auto)-phosphorylation in the complex activation mechanism of LRRK2. Biological Chemistry, 399(7), 643-647. https://doi.org/10.1515/hsz-2017-0332

2017

Liu, Y., Lacal, J., Firtel, R. A., & Kortholt, A. (2018). Connecting G protein signaling to chemoattractant-mediated cell polarity and cytoskeletal reorganization. Small GTPases, 9(4), 360-364. https://doi.org/10.1080/21541248.2016.1235390
Deyaert, E., Wauters, L., Guaitoli, G., Konijnenberg, A., Leemans, M., Terheyden, S., Petrovic, A., Gallardo, R., Nederveen-Schippers, L. M., Athanasopoulos, P. S., Pots, H., Van Haastert, P. J. M., Sobott, F., Gloeckner, C. J., Efremov, R., Kortholt, A., & Versées, W. (2017). A homologue of the Parkinson's disease-associated protein LRRK2 undergoes a monomer-dimer transition during GTP turnover. Nature Communications, 8(1), 1-12. [1008]. https://doi.org/10.1038/s41467-017-01103-4
van Haastert, P. J. M., Keizer-Gunnink, I., & Kortholt, A. (2017). Coupled Excitable Ras and F-actin activation mediate spontaneous pseudopod formation and directed cell movement. Molecular Biology of the Cell, 28(7), 922-934. https://doi.org/10.1091/mbc.E16-10-0733
Xu, X., Wen, X., Veltman, D. M., Keizer-Gunnink, I., Pots, H., Kortholt, A., & Jin, T. (2017). GPCR-controlled membrane recruitment of negative regulator C2GAP1 locally inhibits Ras signaling for adaptation and long-range chemotaxis. Proceedings of the National Academy of Sciences of the United States of America, 114(47), 10092-10101. https://doi.org/10.1073/pnas.1703208114
Hilbi, H., & Kortholt, A. (2017). Role of the small GTPase Rap1 in signal transduction, cell dynamics and bacterial infection. Small GTPases, 10(5), 336-342. https://doi.org/10.1080/21541248.2017.1331721
Deyaert, E., Kortholt, A., & Versées, W. (2017). The LRR-Roc-COR module of the Chlorobium tepidum Roco protein: crystallization and X-ray crystallographic analysis. Acta Crystallographica Section F: Structural Biology Communications , 73(9), 520-524. https://doi.org/10.1107/S2053230X17011955

2016

Liu, Y., Lacal, J., Veltman, D. M., Fusetti, F., van Haastert, P. J. M., Firtel, R. A., & Kortholt, A. (2016). A Gα-Stimulated RapGEF Is a Receptor-Proximal Regulator of Dictyostelium Chemotaxis. Developmental Cell, 37(5), 458-472. https://doi.org/10.1016/j.devcel.2016.05.001
Skoge, M., Wong, E., Hamza, B., Bae, A., Martel, J., Kataria, R., Keizer-Gunnink, I., Kortholt, A., Van Haastert, P. J. M., Charras, G., Janetopoulos, C., & Irimia, D. (2016). A Worldwide Competition to Compare the Speed and Chemotactic Accuracy of Neutrophil-Like Cells. PLoS ONE, 11(6), [e0154491]. https://doi.org/10.1371/journal.pone.0154491
Rosenbusch, K. E., & Kortholt, A. (2016). Activation Mechanism of LRRK2 and Its Cellular Functions in Parkinson's Disease. Parkinsons disease, 2016, [ID 7351985]. https://doi.org/10.1155/2016/7351985
Plak, K., Pots, H., Van Haastert, P. J. M., & Kortholt, A. (2016). Direct Interaction between TalinB and Rap1 is necessary for adhesion of Dictyostelium cells. BMC Cell Biology, 17(1), 1-8. https://doi.org/10.1186/s12860-015-0078-0
Kamp, M. E., Liu, Y., & Kortholt, A. (2016). Function and Regulation of Heterotrimeric G Proteins during Chemotaxis. International Journal of Molecular Sciences, 17(1), [90]. https://doi.org/10.3390/ijms17010090
Guaitoli, G., Raimondi, F., Gilsbach, B. K., Gómez-Llorente, Y., Deyaert, E., Renzi, F., Li, X., Schaffner, A., Jagtap, P. K. A., Boldt, K., von Zweydorf, F., Gotthardt, K., Lorimer, D. D., Yue, Z., Burgin, A., Janjic, N., Sattler, M., Versées, W., Ueffing, M., ... Gloeckner, C. J. (2016). Structural model of the dimeric Parkinson's protein LRRK2 reveals a compact architecture involving distant interdomain contacts. Proceedings of the National Academy of Sciences of the United States of America, 113(30), E4357-E4366. https://doi.org/10.1073/pnas.1523708113
Khanna, A., Lotfi, P., Chavan, A. J., Montaño, N. M., Bolourani, P., Weeks, G., Shen, Z., Briggs, S. P., Pots, H., Van Haastert, P. J. M., Kortholt, A., & Charest, P. G. (2016). The small GTPases Ras and Rap1 bind to and control TORC2 activity. Scientific Reports, 6, 1-9. [25823]. https://doi.org/10.1038/srep25823
Terheyden, S., Nederveen-Schippers, L. M., & Kortholt, A. (2016). The unconventional G-protein cycle of LRRK2 and Roco proteins. Biochemical Society Transactions, 44(6), 1611-1616. https://doi.org/10.1042/BST20160224
Postma, M., & van Haastert, P. J. M. (2016). Mathematics of Experimentally Generated Chemoattractant Gradients. Methods in Molecular Biology, 1407, 381-396. https://doi.org/10.1007/978-1-4939-3480-5_26

2015

Rudi, K., Ho, F. Y., Gilsbach, B. K., Pots, H., Wittinghofer, A., Kortholt, A., & Klare, J. P. (2015). Conformational heterogeneity of the Roc domains in C. tepidum Roc-COR and implications for human LRRK2 Parkinson mutations. Bioscience reports, 35(5), 1-12. [e00254]. https://doi.org/10.1042/BSR20150128
Wu, J., Pipathsouk, A., Keizer-Gunnink, A., Fusetti, F., Alkema, W., Liu, S., Altschuler, S., Wu, L., Kortholt, A., & Weiner, O. D. (2015). Homer3 regulates the establishment of neutrophil polarity. Molecular Biology of the Cell, 26(9), 1629-1639. https://doi.org/10.1091/mbc.E14-07-1197
Terheyden, S., Ho, F. Y., Gilsbach, B. K., Wittinghofer, A., & Kortholt, A. (2015). Revisiting the Roco G-protein cycle. Biochemical Journal, 465(1), 139-147. https://doi.org/10.1042/BJ20141095
Gilsbach, B. K., Messias, A. C., Ito, G., Sattler, M., Alessi, D. R., Wittinghofer, A., & Kortholt, A. (2015). Structural Characterization of LRRK2 Inhibitors. Journal of Medicinal Chemistry, 58(9), 3751−3756. https://doi.org/10.1021/jm5018779

2014

Plak, K., Keizer-Gunnink, I., van Haastert, P. J. M., & Kortholt, A. (2014). Rap1-dependent pathways coordinate cytokinesis in Dictyostelium. Molecular Biology of the Cell, 25(25), 4195-4204. https://doi.org/10.1091/mbc.E14-08-1285
Gilsbach, B. K., & Kortholt, A. (2014). Structural biology of the LRRK2 GTPase and kinase domains: implications for regulation. Frontiers in Molecular Neuroscience, 7, [32]. https://doi.org/10.3389/fnmol.2014.00032
Ho, F. Y., Rosenbusch, K. E., & Kortholt, A. (2014). The Potential of Targeting LRRK2 in Parkinson’s Disease. In A. Qayyum Rana (Ed.), A Synopsis of Parkinson's Disease (pp. 161-192). IntechOpen. https://doi.org/10.5772/57362

2013

Koelsch, V., Shen, Z., Lee, S., Plak, K., Lotfi, P., Chang, J., Charest, P. G., Romero, J. L., Jeon, T. J., Kortholt, A., Briggs, S. P., & Firtel, R. A. (2013). Daydreamer, a Ras effector and GSK-3 substrate, is important for directional sensing and cell motility. Molecular Biology of the Cell, 24(2), 100-114. https://doi.org/10.1091/mbc.E12-04-0271
Kataria, R., Xu, X., Fusetti, F., Keizer-Gunnink, I., Jin, T., van Haastert, P. J. M., & Kortholt, A. (2013). Dictyostelium Ric8 is a nonreceptor guanine exchange factor for heterotrimeric G proteins and is important for development and chemotaxis. Proceedings of the National Academy of Sciences of the United States of America, 110(16), 6424-6429. https://doi.org/10.1073/pnas.1301851110
Plak, K., Veltman, D., Fusetti, F., Beeksma, J., Rivero, F., Van Haastert, P. J. M., & Kortholt, A. (2013). GxcC connects Rap and Rac signaling during Dictyostelium development. BMC Cell Biology, 14(1), 6-1-6-9. [6]. https://doi.org/10.1186/1471-2121-14-6
Kortholt, A., Keizer-Gunnink, I., Kataria, R., & Van Haastert, P. J. M. (2013). Ras activation and symmetry breaking during Dictyostelium chemotaxis. Journal of Cell Science, 126(19), 4502-4513. https://doi.org/10.1242/jcs.132340
Kataria, R., van Haastert, P. J. M., & Kortholt, A. (2013). Reply to Tall et al. Dictyostelium Ric8 does not have a chaperoning function during development and chemotaxis. Proceedings of the National Academy of Sciences of the United States of America, 110(34), E3149-E3149. https://doi.org/10.1073/pnas.1309837110
Mueller-Taubenberger, A., Kortholt, A., & Eichinger, L. (2013). Simple system - substantial share: The use of Dictyosrelium in cell biology and molecular medicine. European Journal of Cell Biology, 92(2), 45-53. https://doi.org/10.1016/j.ejcb.2012.10.003
Veltman, D. M., & Van Haastert, P. J. M. (2013). Extrachromosomal inducible expression. Methods in Molecular Biology, 983, 269-281. https://doi.org/10.1007/978-1-62703-302-2_14

2012

Kortholt, A., van Egmond, W. N., Plak, K., Bosgraaf, L., Keizer-Gunnink, I., & van Haastert, P. J. M. (2012). Multiple Regulatory Mechanisms for the Dictyostelium Roco Protein GbpC. The Journal of Biological Chemistry, 287(4), 2749-2758. https://doi.org/10.1074/jbc.M111.315739
Gilsbach, B. K., Ho, F. Y., Vetter, I. R., van Haastert, P. J. M., Wittinghofer, A., & Kortholt, A. (2012). Roco kinase structures give insights into the mechanism of Parkinson disease-related leucine-rich-repeat kinase 2 mutations. Proceedings of the National Academy of Sciences of the United States of America, 109(26), 10322-10327. https://doi.org/10.1073/pnas.1203223109

2011

Kortholt, A., Kataria, R., Keizer-Gunnink, I., Van Egmond, W. N., Khanna, A., & Van Haastert, P. J. M. (2011). Dictyostelium chemotaxis: essential Ras activation and accessory signalling pathways for amplification. Embo Reports, 12(12), 1273-1279. https://doi.org/10.1038/embor.2011.210
Van Haastert, P. J. M. (2011). Amoeboid Cells Use Protrusions for Walking, Gliding and Swimming. PLoS ONE, 6(11), [e27532]. https://doi.org/10.1371/journal.pone.0027532
Neilson, M. P., Veltman, D. M., van Haastert, P. J. M., Webb, S. D., Mackenzie, J. A., & Insall, R. H. (2011). Chemotaxis: A Feedback-Based Computational Model Robustly Predicts Multiple Aspects of Real Cell Behaviour. PLOS BIOLOGY, 9(5), [1000618]. https://doi.org/10.1371/journal.pbio.1000618
Van Haastert, P. J. M. (2011). How Cells Use Pseudopods for Persistent Movement and Navigation. Science signaling, 4(159), [6]. https://doi.org/10.1126/scisignal.2001708

2010

Kortholt, A., Bolourani, P., Rehmann, H., Keizer-Gunnink, I., Weeks, G., Wittinghofer, A., & Van Haastert, P. J. M. (2010). A Rap/Phosphatidylinositol 3-Kinase Pathway ControlsPseudopod Formation. Molecular Biology of the Cell, 21(6), 936-945. https://doi.org/10.1091/mbc.E09-03-0177
Van Haastert, P. J. M. (2010). A Model for a Correlated Random Walk Based on the Ordered Extension of Pseudopodia. PLoS Computational Biology, 6(8), [e1000874]. https://doi.org/10.1371/journal.pcbi.1000874
Van Haastert, P. J. M. (2010). A Stochastic Model for Chemotaxis Based on the Ordered Extension of Pseudopods. Biophysical Journal, 99(10), 3345-3354. https://doi.org/10.1016/j.bpj.2010.09.042
van Egmond, W. N., & van Haastert, P. J. M. (2010). Characterization of the Roco Protein Family in Dictyostelium discoideum. Eukaryotic Cell, 9(5), 751-761. https://doi.org/10.1128/EC.00366-09
Van Haastert, P. J. M. (2010). Chemotaxis: insights from the extending pseudopod. Journal of Cell Science, 123(18), 3031-3037. https://doi.org/10.1242/jcs.071118
Araki, T., van Egmond, W. N., van Haastert, P. J. M., & Williams, J. G. (2010). Dual regulation of a Dictyostelium STAT by cGMP and Ca2+ signalling. Journal of Cell Science, 123(6), 837-841. https://doi.org/10.1242/jcs.064436
Bosgraaf, L., & Van Haastert, P. J. M. (2010). Quimp3, an automated pseudopod-tracking algorithm. Cell Adhesion & Migration, 4(1), 46-55. https://doi.org/10.4161/cam.4.1.9953

2009

Kortholt, A. (2009). Chemotaxis: a complex netwerk of interconnecting signalling pathways and amplification loops. [S.n.].
Veltman, D. M., Akar, G., Bosgraaf, L., & Van Haastert, P. J. M. (2009). A new set of small, extrachromosomal expression vectors for Dictyostelium discoideum. Plasmid, 61(2), 110-118. https://doi.org/10.1016/j.plasmid.2008.11.003
Veltman, D. M., Keizer-Gunnink, I., & Van Haastert, P. J. M. (2009). An extrachromosomal, inducible expression system for Dictyostelium discoideum. Plasmid, 61(2), 119-125. https://doi.org/10.1016/j.plasmid.2008.11.002
Bosgraaf, L., van Haastert, P. J. M., & Bretschneider, T. (2009). Analysis of Cell Movement by Simultaneous Quantification of Local Membrane Displacement and Fluorescent Intensities Using Quimp2. Cell Motility and the Cytoskeleton, 66(3), 156-165. https://doi.org/10.1002/cm.20338
Van Haastert, P. J. M., & Bosgraaf, L. (2009). Food Searching Strategy of Amoeboid Cells by Starvation Induced Run Length Extension. PLoS ONE, 4(8), [6814]. https://doi.org/10.1371/journal.pone.0006814
Bosgraaf, L., & Van Haastert, P. J. M. (2009). Navigation of Chemotactic Cells by Parallel Signaling to Pseudopod Persistence and Orientation. PLoS ONE, 4(8), [6842]. https://doi.org/10.1371/journal.pone.0006842
Sato, M. J., Kuwayama, H., van Egmond, W. N., Takayama, A. L. K., Takagi, H., van Haastert, P. J. M., Yanagida, T., & Ueda, M. (2009). Switching direction in electric-signal-induced cell migration by cyclic guanosine monophosphate and phosphatidylinositol signaling. Proceedings of the National Academy of Sciences of the United States of America, 106(16), 6667-6672. https://doi.org/10.1073/pnas.0809974106
Van Haastert, P. J. M., & Bosgraaf, L. (2009). The local cell curvature guides pseudopodia towards chemoattractants. HFSP Journal, 3(4), 282-286. https://doi.org/10.2976/1.3185725
Bosgraaf, L., & Van Haastert, P. J. M. (2009). The Ordered Extension of Pseudopodia by Amoeboid Cells in the Absence of External Cues. PLoS ONE, 4(4), [5253]. https://doi.org/10.1371/journal.pone.0005253

2008

Kortholt, A., & van Haastert, P. J. M. (2008). Highlighting the role of Ras and Rap during Dictyostelium chemotaxis. Cellular Signalling, 20(8), 1415-1422. https://doi.org/10.1016/j.cellsig.2008.02.006
Egmond, W. N. V., Kortholt, A., Plak, K., Bosgraaf, L., Bosgraaf, S., Keizer-Gunnink, I., & Haastert, P. J. M. V. (2008). Intramolecular Activation Mechanism of the Dictyostelium LRRK2 Homolog Roco Protein GbpC. The Journal of Biological Chemistry, 283(44). https://doi.org/10.1074/jbc.M804265200
Gotthardt, K., Weyand, M., Kortholt, A., Van Haastert, P. J. M., & Wittinghofer, A. (2008). Structure of the Roc-COR domain tandem of C-tepidum, a prokaryotic homologue of the human LRRK2 Parkinson kinase. EMBO Journal, 27(16), 2239-2249. https://doi.org/10.1038/emboj.2008.150
Veltman, D. M., Keizer-Gunnink, I., & Van Haastert, P. J. M. (2008). Four key signaling pathways mediating chemotaxis in Dictyostelium discoideum. Journal of Cell Biology, 180(4), 747-753. https://doi.org/10.1083/jcb.200709180
Bosgraaf, L., Keizer-Gunnink, I., & van Haastert, P. J. M. (2008). PI3-kinase signaling contributes to orientation in shallow gradients and enhances speed in steep chemoattractant gradients. Journal of Cell Science, 121(21), 3589-3597. https://doi.org/10.1242/jcs.031781
Marin, I., van Egmond, W. N., & van Haastert, P. J. M. (2008). The Roco protein family: a functional perspective. The FASEB Journal, 22(9), 3103-3110. https://doi.org/10.1096/fj.08-111310
Veltman, D. M., & van Haastert, P. J. M. (2008). The role of cGMP and the rear of the cell in Dictyostelium chemotaxis and cell streaming. Journal of Cell Science, 121(1), 120-127. https://doi.org/10.1242/jcs.015602

2007

Keizer-Gunnink, I., Kortholt, A., & Van Haastert, P. J. M. (2007). Chemoattractants and chemorepellents act by inducing opposite polarity in phospholipase C and PI3-kinase signaling. Journal of Cell Biology, 177(4), 579-585. https://doi.org/10.1083/jcb.200611046
Kae, H., Kortholt, A., Rehmann, H., Insall, R., Van Haastert, P. J. M., Spiegelman, G. B., & Weeks, G. (2007). Cyclic AMP signalling in Dictyostelium: G-proteins activate separate Ras pathways using specific RasGEFs. Embo Reports, 8(5), 477-482. https://doi.org/10.1038/sj.embor.7400936
van Haastert, P. J. M., Keizer-Gunnink, I., & Kortholt, A. (2007). Essential role of PI3-kinase and phospholipase A2 in Dictyostelium discoideum chemotaxis. Journal of Cell Biology, 177(5), 809-816. https://doi.org/10.1083/jcb.200701134
Kortholt, A., King, J. S., Keizer-Gunnink, I., Harwood, A. J., & Van Haastert, P. J. M. (2007). Phospholipase C regulation of phosphatidylinositol 3,4,5-trisphosphate-mediated chemotaxis. Molecular Biology of the Cell, 18(12), 4772-4779. https://doi.org/10.1091/mbc.E07-05-0407
Loovers, H. M., Kortholt, A., de Groote, H., Whitty, L., Nussbaum, R. L., & van Haastert, P. J. M. (2007). Regulation of phagocytosis in Dictyostelium by the inositol 5-phosphatase OCRL homolog Dd5P4. Traffic, 8(5), 618 - 628. https://doi.org/10.1111/j.1600-0854.2007.00546.x
Bader, S., Kortholt, A., & van Haastert, P. J. M. (2007). Seven Dictyostelium discoideum phosphodiesterases degrade three pools of cAMP and cGMP. Biochemical Journal, 402(1), 153 - 161. https://doi.org/10.1042/BJ20061153
van Haastert, P. J. M., & Postma, M. (2007). Biased random walk by Stochastic fluctuations of chemoattractant-receptor interactions at the lower limit of detection. Biophysical Journal, 93(5), 1787-1796. https://doi.org/10.1529/biophysj.107.104356

2006

Kortholt, A., Rehmann, H., Kae, H., Bosgraaf, L., Keizer-Gunnink, I., Weeks, G., Wittinghofer, A., & Haastert, P. J. M. V. (2006). Characterization of the GbpD-activated Rap1 Pathway Regulating Adhesion and Cell Polarity in Dictyostelium discoideum. The Journal of Biological Chemistry, 281(33), 23367 - 23376. https://doi.org/10.1074/jbc.M600804200
Bader, S., Kortholt, A., Snippe, H., & van Haastert, P. J. M. (2006). DdPDE4, a novel cAMP-specific phosphodiesterase at the surface of dictyostelium cells. The Journal of Biological Chemistry, 281(29), 20018 - 20026. https://doi.org/10.1074/jbc.M600040200
van Haastert, P. J. M. (2006). Analysis of Signal Transduction: Formation of cAMP, cGMP, and Ins(1,4,5)P3 In Vivo and In Vitro. In L. Eichinger, & F. Rivero (Eds.), Dictyostelium discoideum Protocols (pp. 369 - 392). (Methods in Molecular Biology; No. IV). Springer.
Loovers, H., Postma, M., Keizer-Gunnink, I., Huang, Y. E., Devreotes, P. N., & Haastert, P. J. M. V. (2006). Distinct Roles of PI(3,4,5)P3 during Chemoattractant Signaling in Dictyostelium: A Quantitative In Vivo Analysis by Inhibition of PI3-Kinase. Molecular Biology of the Cell, 17(4), 1503-1513. https://doi.org/10.1091/mbc.E05-09-0825
Veltman, D. M., & van Haastert, P. J. M. (2006). Guanylyl cyclase protein and cGMP product independently control front and back of chemotaxing Dictyostelium cells. Molecular Biology of the Cell, 17(9), 3921 - 3929. https://doi.org/10.1091/mbc.E06-05-0381
Goldberg, J. M., Wolpin, E. S., Bosgraaf, L., Clarkson, B. K., van Haastert, P. J. M., & Smith, J. L. (2006). Myosin light chain kinase A is activated by cGMP-dependent and cGMP-independent pathways. FEBS Letters, 580(8), 2059 - 2064. https://doi.org/10.1016/j.febslet.2006.03.008
Engel, R., van Haastert, P. J. M., & Visser, A. J. W. G. (2006). Spectral characterization of Dictyostelium autofluorescence. Microscopy Research and Technique, 69(3), 168-174. https://doi.org/10.1002/jemt.20282
Bosgraaf, L., & van Haastert, P. J. M. (2006). The regulation of myosin II in Dictyostelium. European Journal of Cell Biology, 85(9-10), 969 - 979. https://doi.org/10.1016/j.ejcb.2006.04.004

2005

Veltman, D. M., Roelofs, J., Engel, R., Visser, A. J. W. G., & Haastert, P. J. M. V. (2005). Activation of soluble guanylyl cyclase at the leading edge during Dictyostelium chemotaxis. Molecular Biology of the Cell, 16(2), 976 - 983. https://doi.org/10.1091/mbc.E04-08-0701
Bukharova, T., Weijer, G., Bosgraaf, L., Dormann, D., van Haastert, P. J., & Weijer, C. J. (2005). Paxillin is required for cell-substrate adhesion, cell sorting and slug migration during Dictyostelium development (vol 118, pg 4295, 2005). Journal of Cell Science, 118(18), 4295 - 4310. https://doi.org/10.1242/jcs.02557
Bosgraaf, L., Waijer, A., Engel, R., Visser, A. J. W. G., Wessels, D., Soll, D., & van Haastert, P. J. M. (2005). RasGEF-containing proteins GbpC and GbpD have differential effects on cell polarity and chemotaxis in Dictyostelium. Journal of Cell Science, 118(9), 1899-1910. https://doi.org/10.1242/jcs.02317
Knol, J., Engel, R., Blaauw, M., Visser, A. J. W. G., & Haastert, P. J. M. V. (2005). The Phosducin-Like Protein PhLP1 Is Essential for Gβγ Dimer Formation in Dictyostelium discoideum. Molecular and Cellular Biology, 25(18), 8393-8400. https://doi.org/10.1128/MCB.25.18.8393-8400.2005

2004

Haastert, P. J. M. V., & Devreotes, P. N. (2004). Chemotaxis: signalling modules join hands at front and tail. Nature Reviews Molecular Cell Biology, 5(8), 626 - 634. https://doi.org/10.1038/sj.embor.7400051
Lu, Y. H., Knol, J. C., Linskens, M. H. K., Friehs, K., van Haastert, P. J. M., & Flaschel, E. (2004). Cultivation of immobilized Dictyostelium discoideum for the production of soluble human Fas ligand. Applied Microbiology and Biotechnology, 65(5), 547 - 552. https://doi.org/10.1007/s00253-004-1620-8
Verheijden, G. F. M., Metz, B., van Ravestein, A. A. M., Bos, E. S., van de Wetering, R., Westerveld, A., Kouwijzer, M., Hanssen, R. G. J. M., van Peij, N. V., van Haastert, P. J. M., Linskens, M. H. K., & van Peij, NN. (2004). Human gonadotropins expressed in dictyostelium discoideum. Biology of Reproduction, 177 - 177.
Engel, R., Hink, M. A., Bosgraaf, L., Haastert, P. J. M. V., & Visser, A. J. W. G. (2004). Pleckstrin Homology Domain Diffusion in Dictyostelium Cytoplasm Studied Using Fluorescence Correlation Spectroscopy. The Journal of Biological Chemistry, 279(11), 10013-10019. https://doi.org/10.1074/jbc.M310039200
Lu, Y. H., Knol, J. C., Linskens, M. H. K., Friehs, K., van Haastert, P. J. M., & Flaschel, E. (2004). Production of the soluble human Fas ligand by Dictyostelium discoideum cultivated on a synthetic medium. Journal of Biotechnology, 108(3), 243-251. https://doi.org/10.1016/j.jbiotec.2003.12.006
Postma, M., Roelofs, J., Goedhart, J., Loovers, H. M., Visser, A. J. W. G., & van Haastert, P. J. M. (2004). Sensitization of Dictyostelium chemotaxis by phosphoinositide-3-kinase-mediated self-organizing signalling patches. Journal of Cell Science, 117(14), 2925-2935. https://doi.org/10.1242/jcs.01143
Veltman, D. M., Bosgraaf, L., & van Haastert, P. J. M. (2004). Unusual guanylyl cyclases and cGMP signaling in Dictyostelium discoideum. Vitamins and Hormones - Advances in Research and Applications, 69, 95-115.

2003

Blaauw, M., Knol, JC., Kortholt, A., Roelofs, J., (Engel), R., Postma, M., Visser, AJWG., Van Haastert, PJM., Knol, J. C., & Visser, A. J. W. G. (2003). Phosducin-like proteins in Dictyostelium discoideum: implications for the phosducin family of proteins. EMBO Journal, 22(19), 5047-5057. https://doi.org/10.1093/emboj/cdg508
Loovers, HM., Veenstra, K., Snippe, H., Pesesse, N. V., Erneux, C., van Haastert, PJM., Loovers, H. M., & Pesesse, X. (2003). A diverse family of inositol 5-phosphatases playing a role in growth and development in Dictyostelium discoideum. The Journal of Biological Chemistry, 278(8), 5652-5658. https://doi.org/10.1074/jbc.M208396200
Roelofs, J., Smith, J. L., & Haastert, P. J. M. V. (2003). cGMP signalling: different ways to create a pathway. Trends in Genetics, 19(3), 132-134. https://doi.org/10.1016/S0168-9525(02)00044-6
Veltman, DM., De Boer, JS., Van Haastert, PJM., & Boer, J. S. D. (2003). Chemoattractant-stimulated calcium influx in Dictyostelium discoideum does not depend on cGMP. Biochimica et Biophysica Acta-General Subjects, 1623(2-3), 129-134. https://doi.org/10.1016/j.bbagen.2003.08.006
Veltman, D., & Van Haastert, PJM. (2003). Regulation of Dictyostelium guanylyl cyclases. Protist, 154(1), 33-42. https://doi.org/10.1078/143446103764928477
Bosgraaf, L., & Haastert, P. J. M. V. (2003). Roc, a Ras/GTPase domain in complex proteins. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1643(1), 5 - 10. https://doi.org/10.1016/j.bbamcr.2003.08.008
Postma, M., Roelofs, J., Goedhart, J., Gadella, TWJ., Visser, AJWG., Van Haastert, PJM., Gadella, T. W. J., & Visser, A. J. W. G. (2003). Uniform cAMP stimulation of Dictyostelium cells induces localized patches of signal transduction and pseudopodia. Molecular Biology of the Cell, 14(12), 5019-5027. https://doi.org/10.1091/mbc.E03-08-0566

2002

Bosgraaf, L., & Haastert, P. J. M. V. (2002). A model for cGMP signal transduction in Dictyostelium in perspective of 25 years of cGMP research. Journal of muscle research and cell motility, 23(7-8), 781-791.
Bosgraaf, L., Russcher, H., Smith, J. L., Wessels, D., Soll, D. R., & Haastert, P. J. M. V. (2002). A novel cGMP signalling pathway mediating myosin phosphorylation and chemotaxis in Dictyostelium. The EMBO Journal, 21(17), 4560-4570. https://doi.org/10.1093/emboj/cdf438
Roelofs, J., & Haastert, P. J. M. V. (2002). Characterization of two unusual guanylyl cyclases from Dictyostelium. The Journal of Biological Chemistry, 277(11), 9167-9174. https://doi.org/10.1074/jbc.M111437200
Roelofs, J., & Haastert, P. J. M. V. (2002). Deducing the origin of soluble adenylyl cyclase, a gene lost in multiple lineages. Molecular Biology and Evolution, 19(12), 2239-2246. https://doi.org/10.1093/oxfordjournals.molbev.a004047
Bosgraaf, L., Russcher, H., Snippe, H., Bader, S., Wind, J., & Van Haastert, PJM. (2002). Identification and characterization of two unusual cGMP-stimulated phoshodiesterases in Dictyostelium. Molecular Biology of the Cell, 13(11), 3878-3889. https://doi.org/10.1091/mbc.E02-05-0302
Goldberg, JM., Bosgraaf, L., Van Haastert, PJM., & Smith, JL. (2002). Identification of four candidate cGMP targets in Dictylostelium. Proceedings of the National Academy of Sciences of the United States of America, 99(10), 6749-6754. https://doi.org/10.1073/pnas.102167299

2001

Postma, M., & Van Haastert, PJM. (2001). A diffusion-translocation model for gradient sensing by chemotactic cells. Biophysical Journal, 81(3), 1314-1323. https://doi.org/10.1016/S0006-3495(01)75788-8
Roelofs, J., & Van Haastert, PJM. (2001). Genomics: Genes lost during evolution. Nature, 411(6841), 1013-1014. https://doi.org/10.1038/35082627
Roelofs, J., Loovers, H., & Van Haastert, PJM. (2001). GTPγS Regulation of a 12-Transmembrane Guanylyl Cyclase Is Retained after Mutation to an Adenylyl Cyclase. The Journal of Biological Chemistry, 276(44), 40740-40745. https://doi.org/10.1074/jbc.M105154200
Roelofs, J., Snippe, H., Kleineidam, RG., & Van Haastert, PJM. (2001). Guanylate cyclase in Dictyostelium discoideum with the topology of mammalian adenylate cyclase. Biochemical Journal, 354(3), 697-706. https://doi.org/10.1042/0264-6021:3540697
Kuwayama, H., Snippe, H., Derks, M., Roelofs, J., & van Haastert, PJM. (2001). Identification and characterization of DdPDE3, a cGMP-selective phosphodiesterase from Dictyostelium. Biochemical Journal, 353(3), 635-644. https://doi.org/10.1042/0264-6021:3530635
Dijken, P. V., & Haastert, P. J. M. V. (2001). Phospholipase Cδ regulates germination of Dictyostelium spores. BMC Cell Biology, 2, [25]. https://doi.org/10.1186/1471-2121-2-25
Potma, E. O., Boeij, W. P. D., Haastert, P. J. M. V., & Wiersma, D. A. (2001). Real-time visualization of intracellular hydrodynamics in single living cells. Proceedings of the National Academy of Sciences, 98(4), 1577 - 1582. https://doi.org/10.1073/pnas.98.4.1577
Potma, E. O., de Boeij, W. P., Bosgraaf, L., Roelofs, J., van Haastert, P. J. M., & Wiersma, D. A. (2001). Reduced protein diffusion rate by cytoskeleton in vegetative and polarized Dictyostelium cells. Biophysical Journal, 81(4), 2010 - 2019. https://doi.org/10.1016/S0006-3495(01)75851-1
Roelofs, J., Meima, M., Schaap, P., & Van Haastert, PJM. (2001). The Dictyostelium homologue of mammalian soluble adenylyl cyclase encodes a guanylyl cyclase. EMBO Journal, 20(16), 4341-4348. https://doi.org/10.1093/emboj/20.16.4341

2000

Blaauw, M., Linskens, MHK., & van Haastert, PJM. (2000). Efficient control of gene expression by a tetracycline-dependent transactivator in single Dictyostelium discoideum cells. Gene, 252(1-2), 71-82. https://doi.org/10.1016/S0378-1119(00)00227-4
Vervoort, E. B., Ravestein, A. V., Peij , van, N., Heikoop, J. C., Haastert, van, P. J. M., Verheijden, G. F., & Linskens, M. H. K. (2000). Optimizing heterologous expression in Dictyostelium: importance of 5 ' codon adaptation. Nucleic Acids Research, 28(10), 2069-2074. https://doi.org/10.1093/nar/28.10.2069

1999

Van Haastert, PJM., & Van Dijken, P. (1999). Biochemistry and genetics of inositol phosphate metabolism in Dictyostelium. In JP. Thiery (Ed.), MOLECULAR MECHANISMS OF TRANSCELLULAR SIGNALING (pp. 75-84). (NATO ADVANCED SCIENCE INSTITUTES SERIES, SERIES A, LIFE SCIENCES; Vol. 309). I O S PRESS.
Van Haastert, PJM., Roelofs, J., & Kuwayama, H. (1999). CGMP as second messenger during Dictyostelium chemotaxis. In JP. Thiery (Ed.), MOLECULAR MECHANISMS OF TRANSCELLULAR SIGNALING (pp. 134-141). (NATO ADVANCED SCIENCE INSTITUTES SERIES, SERIES A, LIFE SCIENCES; Vol. 309). I O S PRESS.
Linskens, M. H. K., Grootenhuis, P. D. J., Blaauw, M., Huisman-de Winkel, B., Ravestein, A. V., Haastert, P. J. M. V., & Heikoop, J. C. (1999). Random mutagenesis and screening of complex glycoproteins: expression of human gonadotropins in Dictyostelium discoideum. European Journal of Biochemistry, 256(6), 639-645.
Last modified:17 March 2016 12.23 p.m.