PhD ceremony: Mr. P.K. Madoori, 12.45 uur, Academiegebouw, Broerstraat 5, Groningen
Dissertation: On the mechanism of promiscuous ligand-binding by multidrug resistance regulator LmrR and structural investigations of lytic transglycosylase MltF
Promotor(s): prof. B.W. Dijkstra
Faculty: Mathematics and Natural Scienes
LmrR (Lactococcal multidrug resistance Regulator) is a protein required by Lactococcus lactis to survive in the presence of toxic compounds. In response to chemically diverse compounds like ethidium and daunomycin, LmrR enhances the production of the major multidrug transporter LmrCD, which excretes the toxic compounds, thus preventing them to reach their cellular targets.
Crystal structures of LmrR in a drug-free state and with several bound drugs, as presented in this thesis, reveal the key structural determinants of its multidrug specificity. The LmrR structure contains a typical β-winged helix-turn-helix domain with an additional C-terminal helix involved in dimerization. Its dimeric organization is highly unusual with a hydrophobic pore at the dimer centre harbouring the multidrug-binding site. Planar lipophilic drugs bind in a similar manner with their aromatic rings sandwiched in between the indole groups of two dimer-related tryptophan residues. The use of a single site for binding different ligands, instead of a large binding site with partially overlapping subsites, and the dominating role of the dyad-related tryptophan residues in drug recognition and binding, distinguish LmrR from other well-characterized multidrug related transcriptional regulators.
Inspired by the importance of the European Wadden Sea for migratory birds, last week China successfully nominated parts of its own Wadden Sea - in the Yellow Sea - for the Unesco World Heritage Status. This means that the list of protected areas is...
The University of Groningen (UG) and Wageningen University & Research (WUR) have decided to work together more closely in the area of nature-inclusive and circular agriculture, and the rural development associated with this, in the Northern Netherlands...
Thirteen researchers from the University of Groningen (UG) and the UMCG have been awarded Veni grants within the framework of NWO’s Innovational Research Incentives Scheme.
A terrific result building on last year's successes, where 12 Groningen researchers...