PhD ceremony: Ms. W. Qu, 14.30 uur, Academiegebouw, Broerstraat 5, Groningen
Dissertation: Analysis of bacterial transmission in contact lens wear and care
Promotor(s): prof. H.C. van der Mei, prof. J.M.M. Hooymans, prof. H.J. Busscher
Faculty: Medical Sciences
Microbial keratitis is a serious complication of wearing contact lenses and can lead to serious visual impairment. Bacterial adhesion to contact lenses is one of the crucial steps in microbial keratitis, which can be regarded as a transmission phenomenon of pathogenic organisms from contact lens case to contact lens to cornea. The aim of this thesis is to develop a method to predict bacterial transmission probabilities based on adhesion force comparisons and to investigate the surface thermodynamic basis for bacterial transmission. Whether or not transmission occurs, depends on the relative magnitude of adhesion forces between causative bacteria with donating lens case and receiving contact lens surface. We first investigated the adhesion forces of bacteria isolated from keratitis to lens case, contact lens and cornea surfaces using Atomic Force Microscopy (AFM), and applied a Weibull analysis on these forces to calculate bacterial transmission probabilities from lens case to cornea with a contact lens as an intermediate. Calculated transmission probabilities coincided well with experimental data, and were in line with surface thermodynamic principles. In addition, preventive measures for the development of contact lens-related infections were analyzed from the transmission perspective, including the influence of Ag-coatings and an anti-adhesive VitroStealth® coating on contact lens cases. Weak adhesion forces and severe bacterial killing were found on Ag-impregnated and VitroStealth® - coated lens cases. These coatings demonstrated to be effective in discouraging microbial adhesion and simultaneously imply better cleaning ability.
Vandaag opent het UMCG de Groningen Microbioom Hub: een nieuwe faciliteit met state-of-the-art technologie waarin alle UMCG-expertise op het gebied van microbioomonderzoek samenkomt. Dit zorgt voor unieke mogelijkheden in onderzoek en maakt nieuwe...
Science often seems very complicated. Most people probably don't really know what researchers do, and what it takes to make a breakthrough. That is why it is important to keep the general public informed of what is happening in science, says Iris...
Het UMCG zet kunstmatige intelligentie (artificial intelligence, afgekort AI) in om zorgverleners te helpen bij het beantwoorden van schriftelijke vragen van patiënten. Dit gebeurt in samenwerking met andere ziekenhuizen vanuit de EPIC Nederlandse...
The UG website uses functional and anonymous analytics cookies. Please answer the question of whether you want to accept
or reject other cookies (such as tracking cookies).
If no choice is made, only basic cookies will be stored. More information