Skip to ContentSkip to Navigation
About us Latest news News News articles

Mapping of asthma susceptibility in recombinant congenic mouse strains

04 April 2012

PhD ceremony: Mr. B.I.A. Piavaux, 12.45 uur, Academiegebouw, Broerstraat 5, Groningen

Dissertation: Mapping of asthma susceptibility in recombinant congenic mouse strains

Promotor(s): prof. A.J.M. van Oosterhout

Faculty: Medical Sciences

Asthma is a pulmonary inflammatory disease in which patients suffer from wheezing and shortness of breath. Like all inflammatory disease it has shown to be influenced by many genes. We used recombinant congenic mouse strains to map asthma susceptibility genes on mouse chromosome 17. Recombinant congenic strains are series of strains which have inherited one or more chromosomal fragments from a donor strain and all other DNA from the background strain. First, we investigated the power of this approach for the mapping of asthma susceptibility using the CcS/Dem series. These strains show different intensities and types of asthmatic response, which indicates that this approach could successfully be used for asthma genetics. We then started to map asthma susceptibility in the Lmr series of recombinant congenic and subcongenic strains. We identified 3 strong asthma susceptibility loci, lmr1A, lmr1B and H2. Lmr1A was further fine-mapped in a F2 cross between the RC strains and their background strain. Based upon the literature, in silico SNP analysis and expression profiling, we were able to identify candidate genes in 2 of these loci: Pla2g7 in lmr1A and Apobec-2 in lmr1B. For the H2 locus different candidate genes remain. Further studies should be undertaken to investigate this. Our models were found to be a valuable tool to investigate this complex locus which, like Pla2g7, was also associated to human asthma in many studies. It also shows that genetic research in mice can generate data relevant to human.

Last modified:13 March 2020 12.59 a.m.
View this page in: Nederlands

More news

  • 29 November 2023

    UMCG opent nieuwe faciliteit voor microbioomonderzoek 

    Vandaag opent het UMCG de Groningen Microbioom Hub: een nieuwe faciliteit met state-of-the-art technologie waarin alle UMCG-expertise op het gebied van microbioomonderzoek samenkomt. Dit zorgt voor unieke mogelijkheden in onderzoek en maakt nieuwe...

  • 28 November 2023

    Science: more exciting than showbiz

    Science often seems very complicated. Most people probably don't really know what researchers do, and what it takes to make a breakthrough. That is why it is important to keep the general public informed of what is happening in science, says Iris...

  • 13 November 2023

    UMCG beantwoordt vragen patiënten met hulp van AI

    Het UMCG zet kunstmatige intelligentie (artificial intelligence, afgekort AI) in om zorgverleners te helpen bij het beantwoorden van schriftelijke vragen van patiënten. Dit gebeurt in samenwerking met andere ziekenhuizen vanuit de EPIC Nederlandse...