PhD ceremony: Mr. J.T. Mika, 12.45 uur, Aula Academiegebouw, Broerstraat 5, Groningen
Dissertation: Molecule diffusion in bacteria and consequences of osmotic stress
Promotor(s): prof. B. Poolman
Faculty: Mathematics and Natural Sciences
The interior of a cell is full of different kinds of (macro)molecules, the crowding of which resembles a shopping mall just a few days before Christmas. The aim of the work of Jacek Mika was to understand how fast molecules move in such an environment. He has presented measurements on the mobility of small, medium and large molecules in the cytoplasm of E. coli. As a tool of manipulating the crowding of cells, he has exposed them to osmotic shifts. When the salt content of the external environment increases, bacterial cells, just like plant cells that have not been watered for a long time, lose water. This results in an increased crowding and a slowing down of the molecule mobility inside cells. An important outcome of the experiments presented in his thesis is that crowding and osmotic stress have more impact on macromolecules than on metabolites. In fact, severe osmotic stress ‘freezes’ proteins as they essentially become immobile. By using a special flow chamber, he has followed the fate of single E.coli cells as they experience osmotic stress. Due to their small size, bacterial cells are difficult to study with light (fluorescence) microscopy. Mika has implemented a new and exciting technique called PALM to perform microscopy of bacteria at increased resolution. This approach allows distinguishing details of structures inside cells that are as small as 10 nm, which is a great improvement as compared to the 250 nm that is the diffraction limit of conventional light microscopy.
Three researchers of the Faculty of Science and Engineering have been awarded an ENW-XS grant by the Dutch Research Council (Nederlandse Organisatie voor Wetenschappelijke Onderzoek, NWO): Prof. A.I. Guskov the Groningen Biomolecular Sciences and...
Bayu Jayawardhana has been appointed scientific director of DISC, the interuniversity research institute and graduate school that unites all university groups in the Netherlands that are active in systems and control theory and engineering. The...
Researchers Clemens Mayer and Danny Incarnato of the University of Groningen have been awarded an ERC Consolidator Grant.
The UG website uses functional and anonymous analytics cookies. Please answer the question of whether you want to accept
or reject other cookies (such as tracking cookies).
If no choice is made, only basic cookies will be stored. More information