PhD ceremony: Ms. E. Papaioannou, 13.15 uur, Aula Academiegebouw, Broerstraat 5, Groningen
Dissertaton: Silencing Pseudomonas virulence
Promotor(s): prof. W.J. Quax
Faculty: Mathematics and Natural Sciences
The use of antibiotics in the treatment of bacterial diseases has led to the emergence of antibiotic-resistant “superbugs”. For preventing this emergence of resistant bacteria, alternative treatment methods are required. Evelina Papaiaoannou invented such a method.
By understanding the mechanism by which the Gram-negative opportunistic pathogen Pseudomonas aeruginosa imposes its virulence, potential targets for alternative antimicrobial therapy to the often-fatal infections caused by this pathogen can be identified. Today, we appreciate that P. aeruginosa imposes its virulence via a tightly controlled cell-to-cell communication mechanism referred to as quorum sensing (QS) through diffusible signal molecules referred to as acyl-homoserine lactones (AHL). Interfering with these signal molecules, either by preventing their synthesis and/or accumulation or by prohibiting their binding to their cognate receptor, offers a promising strategy for controlling and eradicating Pseudomonas-related infections.
Prevention of AHL accumulation can be achieved by the degradation of these molecules. Papaiaoannou made clear that there are two groups of enzymes known to degrade AHLs: (1) AHL-lactonases, which hydrolyse the lactone ring and (2) AHL-acylases, which hydrolyse the amide bond between the acyl chain and the homoserine lactone in the AHL molecule, thus generating the corresponding free fatty acid and the homoserine lactone. Papaiaoannou proved P. aeruginosa PAO1 possesses at least three genes encoding for AHL-acylases that are able to degrade the 3-oxo-C12-HSL signal molecule produced by the pathogen itself. These AHL-acylases are thereby able to silence the communication system of this opportunistic pathogen and may therefore serve as potential antimicrobials against Pseudomonas infections.
NWA Idea Generator project for Kottapalli to develop low-cost sensorized shoes for patients to monitor gait and avoid fall conditions through a biofeedback voice alert.
Lung doctor Sander de Hosson has been elected Alumnus of the Year 2019 by the University of Groningen. He is receiving the award for the incisive way in which he calls for attention for the emotional side of palliative care and for making death a...
The 2020 Gratama Science Award has been awarded to Dr Ymkje Anna de Vries of the Faculty of Behavioural and Social Sciences of the University of Groningen. De Vries followed degree programmes in the fields of neuroscience, clinical science and...