Functional analysis of the YidC homologs of Bacillus subtilis: SpoIIIJ and YqjG
PhD ceremony: Mr. M.J. Saller, 13.15 uur, Doopsgezinde Kerk, Oude Boteringestraat 33, Groningen
Dissertation: Functional analysis of the YidC homologs of Bacillus subtilis: SpoIIIJ and YqjG
Promotor(s): prof. A.J.M. Driessen
Faculty: Mathematics and Natural Sciences
The goal of this thesis was the functional characterization of two membrane-integral proteins of Bacillus subtilis, denoted as SpoIIIJ and YqjG. Unlike other members of the YidC/Oxa1/Alb3 protein family, the B. subtilis homologs were proposed to function in protein secretion rather than membrane protein insertion. SpoIIIJ and YgjG were found to functionally complement the growth defect of an E. coli YidC depletion strain and functional analysis showed that SpoIIIJ and YqjG fulfill a role in membrane protein insertion and assembly. Both proteins could be isolated in complex with the entire F1FO ATP synthase, suggests that these proteins fulfill a role in a late stage of the biogenesis of this complex. Using a membrane proteomic study we could confirm that SpoIIIJ fulfills a specific role in sporulation whereas YqjG could be specifically associated with the development of natural competence in Bacillus subtilis that enables the cells to acquire DNA from the environment. In addition, both YidC proteins play a generic role in the assembly of energy transducing complexes and transport proteins showing that the function of the YidC/Oxa1/Alb3 protein family is highly conserved among organisms.
Last modified: | 13 March 2020 01.12 a.m. |
More news
-
23 April 2024
Nine MSCA Doctoral Network grants for FSE researchers
Nine researchers of the Faculty of Science and Engineering have received a Horizon Europe Marie Sklodowska Curie Doctoral Network grant.
-
22 April 2024
Charissa Roossien secures JTF subsidy to develop Health Tracker
Dr. Charissa Roossien (ENTEG) has successfully secured a Just Transition Fund (JTF) subsidy of 1.8 million euros to develop a Health Tracker for reliable respiratory and metabolic analysis.
-
16 April 2024
UG signs Barcelona Declaration on Open Research Information
In a significant stride toward advancing responsible research assessment and open science, the University of Groningen has officially signed the Barcelona Declaration on Open Research Information.