PhD ceremony: Ms. K.Z du Plessis-Rosloniec, 11.00 uur, Academiegebouw, Broerstraat 5, Groningen
Title: Steroid transformation by Rhodococcus strains and bacterial cytochrome p450 enzymes
Promotor(s): prof. L. Dijkhuizen
Faculty: Mathematics and Natural Sciences
The thesis of Kamila du Plessis-Rosłoniec describes activities of four bacterial steroid-induced cytochrome P450 enzymes in terms of their potential biotechnological use for steroid transformations by engineered Rhodococcus strains into commercially interesting compounds. Three selected rhodococcal P450 enzymes were characterized by her by means of microbiological, genetic, biochemical, spectroscopic and chromatographical methods. Two sterol C26-hydroxylases were shown to be important for 3-hydroxysterol degradation. The role of the novel dextromethorphan N-demethylase in sterol metabolism was analysed. Furthermore, heterologous production of a mutated P450 enzyme from Bacillus megaterium was described in an engineered Rhodococcus strain. The activity of the biocatalyst on 4-androstene-3,17-dione was identified as 16β-hydroxylase by 1H and 13C NMR. The detailed characterization of the cytochrome P450 enzymes, described in the thesis of Du Plessis, provides insight into the catabolic repertoire of Rhodococcus strains, necessary for engineering of steroid catabolic pathways for production of valuable pharmaceuticals.
Three researchers of the Faculty of Science and Engineering have been awarded an ENW-XS grant by the Dutch Research Council (Nederlandse Organisatie voor Wetenschappelijke Onderzoek, NWO): Prof. A.I. Guskov the Groningen Biomolecular Sciences and...
Bayu Jayawardhana has been appointed scientific director of DISC, the interuniversity research institute and graduate school that unites all university groups in the Netherlands that are active in systems and control theory and engineering. The...
Researchers Clemens Mayer and Danny Incarnato of the University of Groningen have been awarded an ERC Consolidator Grant.
The UG website uses functional and anonymous analytics cookies. Please answer the question of whether you want to accept
or reject other cookies (such as tracking cookies).
If no choice is made, only basic cookies will be stored. More information