The influence of peptide structure on fragmentation pathways
PhD ceremony: Ms. S. Bari, 11.00 uur, Academiegebouw, Broerstraat 5, Groningen
Title: The influence of peptide structure on fragmentation pathways
Promotor(s): prof. R. Hoekstra
Faculty: Mathematics and Natural Sciences
The current increasing social and corporate interest in proton- and heavy ion-therapy of malignant tumours now results in the construction of first particle treatment centres. The chemical and biological aspects of biological radiation damage have been studied in great detail. However, little is known about biological radiation action on the molecular level. Experiments with small biomolecules in the gas phase have the advantage of studying ionization and fragmentation dynamics in finite systems but are less realistic radiation damage models. To be able to investigate more complex biomolecular systems, such as large proteins, we have developed a new apparatus in which a home built electrospray source is interfaced with a low energy (keV) ion beamline or a VUV photon beamline.
Being a pioneering study, this thesis aims at paving the way for studies on radiation upon complex biomolecule by an in depth investigation of ion- and photo-induced fragmentation of the relatively small model-peptide leucine enkephalin. Additionally, systematic fragmentation studies on different amino acids were performed. The central theme of the performed studies is the influence of the molecular structure on the fragmentation pathways upon ion or photon impact.
An explicit dependence of the fragmentation of leucine-enkephalin on the projectile parameters is observed and the distinguishing feature of amino acids, the sidechain, dominates the peptide spectra.
Last modified: | 13 March 2020 01.16 a.m. |
More news
-
23 April 2024
Nine MSCA Doctoral Network grants for FSE researchers
Nine researchers of the Faculty of Science and Engineering have received a Horizon Europe Marie Sklodowska Curie Doctoral Network grant.
-
22 April 2024
Charissa Roossien secures JTF subsidy to develop Health Tracker
Dr. Charissa Roossien (ENTEG) has successfully secured a Just Transition Fund (JTF) subsidy of 1.8 million euros to develop a Health Tracker for reliable respiratory and metabolic analysis.
-
16 April 2024
UG signs Barcelona Declaration on Open Research Information
In a significant stride toward advancing responsible research assessment and open science, the University of Groningen has officially signed the Barcelona Declaration on Open Research Information.