PhD ceremony: Ms. D.S.M. Mueller, 13.15 uur, Academiegebouw, Broerstraat 5, Groningen
Thesis: The effects of urea and of pH on protein structure
studied by molecular dynamics simulation
Promotor(s): prof. A.E. Mark
Faculty: Mathematics and Natural Sciences
The effects of urea and of pH on protein structure studied by molecular dynamics simulation In my dissertation I present two studies in computational chemistry. The first examines the partial unfolding of an enzyme in urea. The enzyme cytochrome c reacts with peroxides, but normally this “peroxidase” property is weak. However, the solvation in urea leads to a 1000-fold increase of the peroxidase activity. Urea loosens the structure of the protein and thus makes the active site inside the protein more accessible for the peroxides, leading to a higher reaction rate. My simulations of cytochrome c in urea showed the partial opening of the reactive site. At the same time the overall structure of the protein remained intact, allowing it to return to its original, less active state when the concentration of urea is lowered. This may enable the recycling, stable storage and multiple activation of cytochrome c peroxidase for industrial application. The second study investigates the structural changes of a viral protein under acidic conditions. Many viruses are enveloped hollow particles covered with proteins. At acidic pH these proteins transform and enable the virus to merge with the host cell and infect the host. Histidine is a small component of proteins that is sensitive to the acidic pH at which the infection takes place. My simulations of a viral envelope protein showed that the local changes of the histidines at acidic pH are capable of triggering irreversible and global rearrangements in the protein. These may be important for the merger of the virus with the host cell and therefore for infection.
The timing, route, and destination for godwit migration is learned rather than innate. Researchers at the University of Groningen discovered this in a daring experiment, which has been published in the latest issue of the journal Current Biology.
The grant is worth EUR 500,000, of which Avraamidou and Sburlea receive around EUR 100,000.
George Azzopardi and Guru Swaroop Bennabhaktula from the Faculty of Science and Engineering have won the Ben Feringa Impact Award 2023 for their project ‘4NSEEK; Forensic Against Sexual Exploitation of Children’. In the ‘students’ category, Nine van...
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