Skip to ContentSkip to Navigation
About us Latest news News News articles

A proteomics approach to inner membrane biogenesis in Escherichia coli

26 February 2010

Promotie: mw. C.E. Price, 16.15 uur, Academiegebouw, Broerstraat 5, Groningen
Proefschrift: A proteomics approach to inner membrane biogenesis in Escherichia coli
Promotor(s): prof.dr. A.J.M. Driessen
Faculteit: Wiskunde en Natuurwetenschappen
Contact: Claire Price, tel. 050-368 2415, e-mail:

A proteomics approach to inner membrane biogenesis in Escherichia coli

Claire Price investigated the role of the Escherichia coli YidC protein in membrane biogenesis with a focus on subunits of respiratory complexes. A directed proteomics approach was applied to investigate the effect of YidC depletion on components of the respiratory chains expressed under anaerobic conditions. It was found that YidC depletion under these conditions caused a cessation in growth and that a number of respiratory complexes were negatively affected. This was further investigated, where metabolic labeling with 15N/14N was employed to study the membrane proteome-wide changes upon YidC depletion under both aerobic and anaerobic respiratory conditions. This allowed the identification of putative YidC substrates and the in vitro insertion requirements of subunit K of complex I were characterized. It was found that NuoK absolutely requires the Sec translocase and YidC for insertion. Essential membrane-embedded negative charges contained in NuoK were shown to confer YidC-dependence. Using the mechanosensitive channel of large conductance, MscL, it was shown further that in vitro translation and membrane insertion systems can be exploited to produce fully active oligomeric protein complexes in a cell-free system. Since YidC has been shown to interact with the SecDFYajC complex the effect of SecDFYajC depletion on the membrane proteome of E. coli was also investigated. The findings are discussed in the context of our current knowledge of the Oxa1/Alb3/YidC family of proteins with particular focus on the diverse functioning of YidC in membrane biogenesis in E. coli.

Last modified:13 March 2020 01.14 a.m.
View this page in: Nederlands

More news

  • 13 October 2023

    Moniek Tromp appointed Captain of Science of the Top Sector Chemistry

    Prof. Moniek Tromp has been appointed Captain of Science of the Chemistry Top Sector by the Minister of Economic Affairs and Climate Policy. As from 1 July 2023, she succeeded Prof. Bert Weckhuysen from Utrecht University.

  • 12 September 2023

    Art in times of AI

    Leonardo Arriagada Beltran conducted his PhD research on the interface of computer-generated art and the constantly evolving field of Artificial Intelligence (AI). He will defend his Phd thesis on 21 September. His research offers valuable insights...

  • 28 August 2023

    Harish Vedantham and Casper van der Kooi nominated for 'Wetenschapstalent 2023'

    Harish Vedantham and Casper van der Kooi have been nominated by New Scientist for Wetenschapstalent 2023 (Science Talent 2023). This election is meant to give young scientists and their research a stage.