Research Highlights

Dr. Max Fürst

Three top publications 2017-2022

1. Fürst MJLJ, Boonstra M, Bandstra S & Fraaije MW (2019) Stabilization of cyclohexanone monooxygenase by computational and experimental library design. Biotechnology and Bioengineering 116 (9): 2167-2177; DOI:https://doi.org/10.1002/bit.27022

A protein engineering study on an industrially-relevant oxidative biocatalyst showcasing the power of combined computational mutation predictions and plate-based activity screens.

2. Fürst MJLJ, Romero E, Gómez Castellanos JR, Fraaije MW & Mattevi A (2019) Side-chain pruning has limited impact on substrate preference in a promiscuous enzyme. ACS Catalysis 12 (8): 11648-11656; DOI: https://doi.org/10.1021/acscatal.8b03793

An example of how protein engineering is used to understand the intriguing mechanisms of enzyme catalysis. We show that active-site residues in cofactor-containing enzymes converting hydrophobic substrates are far less important than previously thought.

3. Korbeld KT & Fürst MJLJ (2023) Curse and blessing of non-proteinogenic parts in computational enzyme engineering. ChemBioChem: e202300192; DOI: https://doi.org/10.1002/cbic.202300192

The first publication of the Fürst lab is a guide for successful computational protein engineering of enzymes with non-proteinogenic parts, such as cofactors, metals, or non-canonical amino acids.