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About us Medical Sciences Research Microscopy Centre
University Medical Center Groningen

TissueFaxs

TissueFaxs
TissueFaxs

TissueGnostics TissueFAXS

This system combines detailed morphologic information of tissues and single cells offered by microscopy with the accuracy of multiparameter flowcytometry allowing quantitative expression measurements. Based on a high-end fully motorized Zeiss AxioObserver Z1 microscope

STAGE

◘ ultra precise automated Märzhäuser object table

◘ inserts for 12 separate slides or one multi (6 or 96) well plate

◘ pre-programmed for TMA analysis

◘ temperature, CO2 and O2 controlled live cell imaging insert  (PeCon)

AVAILABLE OBJECTIVES

◘ Zeiss-objectief EC "Plan-Neofluar" 2.5x/0.075 Dry

◘ Zeiss-objectief EC "Plan-Neofluar" 10x/0,3 Dry, Ph1

◘ Zeiss-objectief LD "Plan-Neofluar" 20x/0,4 Corr Dry, Ph2

◘ Zeiss-objectief EC "Plan-Neofluar" 40x/1,30 Oil, DIC

◘ Zeiss-objectief "Plan-Apochromat" 63x/1,40 Oil, DIC

LIGHT SOURCES

◘ Zeiss LED  Brightfield illumination

◘ EXFO Excite 120 PC fluorescence illumination.

FILTERS

◘ Zeiss-filter set 47 CFP shift free voor FRET

◘ Zeiss-filter set 46 YFP shift free voor FRET

◘ Zeiss-filter set 48 FRET CFP/YFP shift free voor FRET

◘ Zeiss Bleach Set 01 YFP voor FRET

Chroma ET Dapi-filter set  49000

Chroma ET FITC/GFP-filter set   49002

Chroma ET CY3-filter set   49004  

Chroma ET Cy5-filter set   49006

Chroma FITC-   filter set   49011  

Chroma TXred/mCherry-   filter set     49008

CAMERAS

◘ Monochrome camera system PCO Pixelfly II  1.4 Megapixels

◘ CMOS-color camera PL-B623 Pixelink 3.1 Megapixels

SOFTWARE

◘ TissueFaxs acquisition software

◘ TissueQuest fluorescence analysis software

◘ HistoQuest histology analysis software

Key features: fully automated slide scanning, multi-channel acquisition, generation of high-resolution overviews by Stitching, selectable ROI’s, powerful algorithms for image processing, automated identification of cells, analysis at the single cell level, FACS-style dot-plot operations, backward gating from dot-to-cell, storage of virtual slide data and analyzed results.

An extra workstation for the off-line analysis is available

See also the TissueGnostics website for more information on the system.

Laatst gewijzigd:22 april 2016 16:22