Equipment

Microscopes:

◘ LEICA TCS SP2 aobs high resolution confocal laser scan microscope (specifications)

◘ SOLAMERE spinningdisk laser scan microscope (specifications)

◘ TissueGnostics TissueFAXS automated analysis system (specifications)

◘ Zeiss LSM 780 two photon/single photon confocal laser scan microscope (specifications)

◘ Zeiss LSM 7MP upright two photon only confocal laser scan microscope (specifications)

◘ Leica TCS SP8 high resolution confocal laser scan microscope (specifications)

◘ Deltavision Elite integrated fast and high-resolution fluorescence microscope . (specifications)

            (All above mentioned microscopes are installed in ML1  approved rooms.)

◘ Olympus BX 50 histology microscope. With high resolution digital color camera.

  2x, 4x, 10x, 20x, 40x, 100x(oil) objectives. Ph1, Ph2, DIC, polarisation. Upright, discussion optics.

◘ Leica DM4000B routine Fluorescence microscope (Upright) with digital Leica DFC3000 G camera.

  2.5x, 5x, 10x, 20x, 40x( 1.0 Fluotar, oil), 40X Waterdipping, 63X(1.4 Plan APO, oil)objectives. Leica Filters: DAPI ET, TXR ET, Y5 ET, Y3 ET, L5 ET.

LAS 4.3 with auto overlay. This microscope is refurbished in September 2013.

             

Image Processing:

Umic has 4 workstations available for data analysis. They can be used free of charge by registered users.

◘ Quad core 64 bits Windows XP: (room1)

  Installed software:   ImageJ /FIJI

                                Huygens Proffesional (deconvolution)

                                Photoshop CS5

                                 Imaris (3D viewing only)

                                MatLab

                                Scanscope      

◘ Xeon 12 core 64 bits W7:(room 1)

  Installed software: ImageJ /FIJI

                              MS Office

                              ZEN Black / ZEN Blue

                              Photoshop CS5

                              Imaris (3D rendering basic)

                            Scanscope

◘ i7 6 Core 64bits W7   (room 2)

    Installed software: ImageJ/FIJI

                                Imaris (7.6) tracking, filament tracer, XT (3d rendering and analysis)                          

                                Photo Shop CS5

                                MS Office

                                MatLab

                                Scanscope

◘ Quad core 32 bits W7 (room3)

    Installed software: Tissue Quest 3

                               TissueQuest 4

                                Histo Quest

                                ImageJ /FIJI

                                Open Office

                                  Photo shop    

                                  Ms Office

◘ Dual core 32bits XP (room6)

Installed software:ImageJ, Photoshop CS4, MS Office

Attached to the Zeiss Stemi Correlative station

    (the use of the workstations, the Leica DM4000 and the Olympus BX50 is free of charge)

About software:

◘ ImageJ (NIH, freeware, several versions) scientific image processing. Many plugins and macro's.

◘ Huygens deconvolution (SVI), for removing out of focus blurring, noise in confocal and Nipkow disk images (only on computer no1). Also colocalisation analysis.

◘ Imaris 7.0 (Bitplane) 3D reconstruction. With this program you can easily load, visualize and analyze data from a few megabytes to multiple gigabytes in size from almost all microscope image formats. 3D reconstructions, particle tracking, filament tracer and more. (only on computer no2, the lite version on no1)

◘ Photoshop (Adobe). Photo editor

◘ ZEN Zeiss output evaluation

◘ LCS lite (Leica)  For easy evaluation of Leica images.

◘ HistoQuest (Tissuegnostics)  to identify and analyze individual cells in their native tissue environment. For histological data acquired with the TissueFaxs microscope or a Hammamatsu system.

◘ TissueQuest (Tissuegnostics)  As HistoQuest, for fluorescent labelled samples.