
Equipment

Microscopes:
◘ LEICA TCS SP2 aobs high resolution confocal laser scan microscope (specifications)
◘ SOLAMERE spinningdisk laser scan microscope (specifications)
◘ TissueGnostics TissueFAXS automated analysis system (specifications)
◘ Zeiss LSM 780 two photon/single photon confocal laser scan microscope (specifications)
◘ Zeiss LSM 7MP upright two photon only confocal laser scan microscope (specifications)
◘ Leica TCS SP8 high resolution confocal laser scan microscope (specifications)
◘ Deltavision Elite integrated fast and high-resolution fluorescence microscope . (specifications)
(All above mentioned microscopes are installed in ML1 approved rooms.)
◘ Olympus BX 50 histology microscope. With high resolution digital color camera.
2x, 4x, 10x, 20x, 40x, 100x(oil) objectives. Ph1, Ph2, DIC, polarisation. Upright, discussion optics.
◘ Leica DM4000B routine Fluorescence microscope (Upright) with digital Leica DFC3000 G camera.
2.5x, 5x, 10x, 20x, 40x( 1.0 Fluotar, oil), 40X Waterdipping, 63X(1.4 Plan APO, oil)objectives. Leica Filters: DAPI ET, TXR ET, Y5 ET, Y3 ET, L5 ET.
LAS 4.3 with auto overlay. This microscope is refurbished in September 2013.
Image Processing:
Umic has 4 workstations available for data analysis. They can be used free of charge by registered users.
◘ Quad core 64 bits Windows XP: (room1)
Installed software: ImageJ /FIJI
Huygens Proffesional (deconvolution)
Photoshop CS5
Imaris (3D viewing only)
MatLab
Scanscope
◘ Xeon 12 core 64 bits W7:(room 1)
Installed software: ImageJ /FIJI
MS Office
ZEN Black / ZEN Blue
Photoshop CS5
Imaris (3D rendering basic)
Scanscope
◘ i7 6 Core 64bits W7 (room 2)
Installed software: ImageJ/FIJI
Imaris (7.6) tracking, filament tracer, XT (3d rendering and analysis)
Photo Shop CS5
MS Office
MatLab
Scanscope
◘ Quad core 32 bits W7 (room3)
Installed software: Tissue Quest 3
TissueQuest 4
Histo Quest
ImageJ /FIJI
Open Office
Photo shop
Ms Office
◘ Dual core 32bits XP (room6)
Installed software:ImageJ, Photoshop CS4, MS Office
Attached to the Zeiss Stemi Correlative station
(the use of the workstations, the Leica DM4000 and the Olympus BX50 is free of charge)
About software:
◘ ImageJ (NIH, freeware, several versions) scientific image processing. Many plugins and macro's.
◘ Huygens deconvolution (SVI), for removing out of focus blurring, noise in confocal and Nipkow disk images (only on computer no1). Also colocalisation analysis.
◘ Imaris 7.0 (Bitplane) 3D reconstruction. With this program you can easily load, visualize and analyze data from a few megabytes to multiple gigabytes in size from almost all microscope image formats. 3D reconstructions, particle tracking, filament tracer and more. (only on computer no2, the lite version on no1)
◘ Photoshop (Adobe). Photo editor
◘ ZEN Zeiss output evaluation
◘ LCS lite (Leica) For easy evaluation of Leica images.
◘ HistoQuest (Tissuegnostics) to identify and analyze individual cells in their native tissue environment. For histological data acquired with the TissueFaxs microscope or a Hammamatsu system.
◘ TissueQuest (Tissuegnostics) As HistoQuest, for fluorescent labelled samples.
Laatst gewijzigd: | 08 november 2017 11:14 |