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University Medical Center Groningen

Miltenyi Biotec MACSQuant

Miltenyi Biotec MACSQuant
Miltenyi Biotec MACSQuant

General Information

In the FCU ( Flow Cytometry Unit ) one MACSQuant system is located. The MACSQuant system is used for both education and research purposes.

The MACSQuant is a compact bench top flowcytometer with three lasers (40, 488 and 640 nm) that provide the ability to analyze up to 9 parameters (FSC, SSC and seven colors (4 on the 488 laser, 2 on the 640 laser and 1 on the 405 laser). Also pulse processing (height, width and area) can be measured on all the parameters. The fluidics can measure at high (100 µl/min), medium (50 µl/min), and low (25 µl/min) flow rates. In addition to this absolute cell counting is possible (volumetric with a syringe pump). So there is no need of counting beads.

Besides manual delivering the sample to the MACSQuant for acquisition, multisampling using the MiniSampler can be performed. The MiniSampler can hold tube racks and varying formats of microtiter plates (96 well plates)

Samples are acquired (16 bits digitilizations in 5 decades) using the MACSQuantify software. Samples are saved as FCS3.0 files. Compensation afterwards is possible.

Specifications

Lasers

Laser

Wavelength

Optical Power (at the flowcell)

Violet

405 nm

40 mW

Blue (diode pumped solid state)

488 nm

30 mW

Red

635 nm

20 mW

Standard Configuration

Detector

Filter

Fluorochrome (s)

FSC

488/10

-

SSC

488/10

-

FL - 1

450/50

V450, Pacific Blue, BV421, DAPI, E450 (NC), Vivid, FxCycle Violet

FL - 2

525/50

FITC, GFP, Alexa 488, CFSE, CFDA, FAM, Picogreen

FL - 3

585/40

PE, PI

FL - 4

655-730 (655LP +split 730)

PI, PE-Cy5, Pe-Cy5.5, PerCP, PerCP-Cy5.5

FL - 5

750LP

PE-Cy7

FL - 6

655-730 (655LP + split 730)

APC, Alexa 633, Alexa 647, Cy5, Alexa 700

FL - 7

750LP

APC-Cy7, APC-H7, APC-A750, APC E780

Sample Format

Sample format

Manual port

5 ml tubes

Chill 5 rack (Using MiniSampler)

24 x 5ml tubes

Chill 96 rack (Using MiniSampler)

96-well microtiter plates

Last modified:15 October 2019 1.28 p.m.