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University Medical Center Groningen

BD LSR-II

BD LSR-II
BD LSR-II

General Information

In the FCU (Flow Cytometry Unit) one BD LSR-II system is located. The BD LSR-II system is used for research purposes.

The BD LSR-II cytometer is a research flow cytometer with 5 lasers that provides the ability to analyze up to 20 parameters (FSC, SSC and 18 colors ). On these 5 lasers we can measure different fluorochromes (2 on Blue, 3 on Red, 6 on Violet, 4 on Yellow-Green and 3 on the UV-laser ). Bandpass and Dichroic filters can be changed with help of one of the operators (This can only be done by the user themselves after permission of the facility). Also Pulse processing (height, width and area) can be measured on all the parameters. The pressure driven fluidics can measure at high (120 ul/min), medium (60 ul/min), and low (12 ul/min) flow rates (fine adjustment is possible).

Samples are acquired (18 bits digitalization in 5 decades) using DIVA 8.0 software. Samples are saved as FCS 3.0 or 3.1 files. Compensation afterwards is always possible.

The LSR-II is located at the FCU (second door left)

Specifications

Lasers

Laser

Wavelength

Optical Power (at the flowcell)

Ultraviolet

355 nm

18 mW

Violet

405 nm

40 mW

Blue

488 nm

20 mW

Yellow-Green

561 nm

40 mW

Red

635 nm

35 mW

Standard configuration

355 nm Laser

Detector

Filter

Fluorochrome (s)

UV - A

386/23

BUV395

UV - B

586/20-25SR

BUV563

UV - C

740/40 BUV737

405 nm Laser

Detector

Filter

Fluorochrome (s)

Violet - A

785/60

Qdot 800, BV785

Violet - B

615/20

BV605, eFluor 605

Violet - C

586/15

BV570, Pacific Orange

Violet - D

520/30

Pacific Orange, Qdot 525, V500, BV510

Violet - E

460/36

Pacific Blue, V450, DAPI, eFluor 450 (NC)

Violet - F

430/24

BV421

488 nm Laser

Detector

Filter

Fluorochrome (s)

FSC

488/10

-

Blue - A

695/40

PerCP, PerCP-Cy5.5

Blue - B

530/30

Alexa Fluor 488, FITC, GFP, CFSE, PicoGreen, CFDA, FAM

Blue - C

488/10

SSC

561 nm Laser

Detector

Filter

Fluorochrome (s)

Yellow-Green - A

785/62

PE-Cy7

Yellow-Green - B

710/50

PE-Alexa 700, PE-Cy5.5

Yellow-Green - C

670/30

CyChrome, PE-Cy5, 7-AAD, CyQ

Yellow-Green - D

615/20

PE-Alexa 610, PE-Texas Red, PI, mCherry, mRaspberry, PE-CF594, ECD

Yellow-Green - E

585/15

dsRed, PE, dTomato, mBanana

635 nm Laser

Detector

Filter

Fluorochrome (s)

Red - A

780/60

APC-Cy7, APC-H7, APC-Alexa Fluor 750, APC-eFluor 780

Red - B

730/45

Alexa Fluor 700, APC-Alexa 700

Red - C

660/20

Alexa Fluor 647, Alexa Fluo3 633, APC, Cy5, ToPro-3

Sample Format

Sample format

Manual port

5 mL tubes (12 x 75-mm)

Last modified:15 October 2019 1.19 p.m.