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About us Medical Sciences Research Flow Cytometry Unit Instruments
University Medical Center Groningen

BD FACSVerse

BD FACSVerse
BD FACSVerse

General Information

In the FCU (Flow Cytometry Unit) one BD FACSVerse system is located. The BD FACSVerse system is used for research purposes.

The BD FACSVerse cytometer is a compact research flow cytometer with three lasers (405, 488 and 640 nm) that provide the ability to analyze up to 10 parameters (FSC, SSC and 8 colors ( 4 on the 488 laser, 2 on the 640 laser and 2 on the 405 laser ). Emission filters can be changed and filters are automatic detected. Also pulse processing (height, width and area) can be measured on all the parameters. The vacuum driven fluidics can measure at high (120 µl/min), medium (60 µl/min), and low (12 µl/min) flow rates.

Besides manual delivering the sample to the BD FACSVerse cytometer for acquisition, multisampling of samples using the BD FACS Universal Loader can be performed by using 30- and 40- tube racks (5 ml tubes) or multiple types of microtiter plates (96 wells plates)
Samples are acquired ( 18 bits digitalization in 5 decades) using the FACSuite software. Samples are saved as FCS3.0 files by which compensation can even be changed after measurement.

By using the BD FACSuite CS&T research beads, cytometer performance is checked. Automatic adjustments, ensuring consistent values from day to day and experiment to experiment is possible by calibrating with the CS&T beads (Reference or tube settings can be updated with CST beads to correct for instrumental variations)

Specifications

Lasers

Laser

Wavelength

Optical Power (at the flowcell)

Violet

405 nm

40 mW

Blue

488 nm

20 mW

Red

640 nm

40 mW

Standard Configuration (BV605)

405 nm Laser

Detector

Filter

Fluorochrome

Violet - A

613/18

BV605, E605, E605 NC

Violet - B

448/45

V450, Pacific Blue, BV421, Vivid, E450 (NC), FXCycle violet, DAPI

488 nm Laser

Detector

Filter

Fluorochrome

Blue - A

783/56

PE-Cy7

Blue - B

700/54

PerCP-Cy5.5, Propidium Iodide, PerCP, PerCP E710, 7-AAD, PE-Cy5, LDS751

Blue - D

586/42

PE, PI

Blue - E

527/32

FITC, Alexa 488, CFSE, EGFP, PicoGreen, CFDA, FAM

Blue - F

488/15

SSC

635 nm Laser

Detector

Filter

Fluorochrome

Red - A

783/56

APC-Cy7, APC-H7, APC-E780, APC-A750

Red - B

660/10

APC, Alexa 633, Alexa 647, Cya5

Additional Filter(s)

Mirror

Filter

Fluorochrome

535LP

545/20

YFP

500LP

528/45

V500, Hoechst, Pacific Orange, AmCyan

705LP

720/30

Alexa 700

510/10

510/10

GFP

Sample Formats

Sample format(s)

Manual port

5 mL (12 x 75-mm) tubes, BD Trucount™ 5 mL (12 x 75-mm), 15 mL tubes, 50 mL tubes, Eppendorf cups 2 mL

Tube racks

30-tube rack (12 x 75-mm tubes), 40-tube rack (12 x 75-mm tubes)

Plates

96 standard height, round, polystyrene, 96 standard height, flat, polystyrene, 96 standard height, round, polypropylene, 96 standard height, conical, polypropylene, 384 standard height, flat, polystyrene, 96, half deep, conical, polypropylene, 96, deep, conical, polypropylene, 96, matrix tube, 96, filter bottom, polypropylene

Last modified:15 October 2019 1.19 p.m.