T. (Tushar) Tomar

Research

Research interests

Cancer biologist with training in translational medicine research focusing on drug-resistance in gynecological cancers

Dry-lab work :

• Ability to design and perform all data analysis of high throughput sequencing experiments (Microarrays, Next generation sequencing, Infinium 27K/450K methylation arrays, SNP arrays) on patient tumor material

• Skilled in BIG Data analysis and in-silico validation using publically available datasets

• Competent in generating hypothesis based on patient cohorts genomic data including targets identification and their in-silico validation.

Wet-lab work :

• Proficient in designing primers and performing cancer epigenomics assays including  pyrosequencing, bisulphite-sequencing, methylation-specific PCR and quantitative-rtPCR

• Extensive experienced in designing and executing gene knock-in and knock-down (transient and stable) experiments using RNAi / shRNA / pcDNA (ORF construct)

• Experienced in all molecular and analytical techniques: various primary and immortalized cell line culturing, westernblotting, immuno and fluorescence staning, analytic quantitative assays like ELISA, FACS; drug cytotoxicity assays like MTT; Alamar Blue; in-vitro functional assays like proliferation, migration and invasion assays; and cell cycle analysis.

• Gained wealth of experience in establishing and studying cell-line derived and patient-derived xenograft (PDX) cancer mouse models.

• Competent in target specific in-vivo molecular optical imaging

Publications
  1. Integrative kinome exome sequencing and copy number profiling of ovarian clear cell carcinoma

    Caumanns, J. J., Wisman, G. B. A., Berns, K., Tomar, T., Fehrmann, R. S. N., Bernards, R., Van der Zee, A. G. J. & De Jong, S. Jul-2017 In : Cancer Research. 77, 13 Supplement, 1219

    Research output: ScientificMeeting Abstract

  2. Methylome analysis of extreme chemoresponsive patients identifies novel markers of platinum sensitivity in high-grade serous ovarian cancer

    Tomar, T., Alkema, N. G., Schreuder, L., Meersma, G. J., Meyer, T., Van Criekinge, W., Klip, H. G., Fiegl, H., Van Nieuwenhuysen, E., Vergote, I., Widschwendter, M., Schuuring, E., van der Zee, A. G. J., de Jong, S. & Wisman, G. B. A. 23-Jun-2017 In : BMC Medicine. 15, 1, p. 116 16 p., 116

    Research output: Scientific - peer-reviewArticle

  3. Protective effect of rosiglitazone on kidney function in high-fat challenged human-CRP transgenic mice: A possible role for adiponectin and miR-21?

    Morrison, M. C., Yakala, G. K., Liang, W., Wielinga, P. Y., Salic, K., van Koppen, A., Tomar, T., Kleemann, R., Heeringa, P. & Kooistra, T. Jun-2017 In : Scientific Reports. 7, 10 p., 2915

    Research output: Scientific - peer-reviewArticle

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