prof. dr. J.P. (John) LaCava

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E-mail:
j.p.lacava umcg.nl

Research

  1. 2020
  2. LaCava, J., & Vaňáčová, Š. (Eds.) (2020). The Eukaryotic RNA Exosome: Methods and Protocols. (Methods in Molecular Biology ; Vol. 2062). Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9822-7
  3. 2019
  4. Ardeljan, D., Wang, X., Oghbaie, M., Taylor, M. S., Husband, D., Deshpande, V., Steranka, J. P., Gorbounov, M., Yang, W. R., Sie, B., Larman, H. B., Jiang, H., Molloy, K. R., Altukhov, I., Li, Z., McKerrow, W., Fenyo, D., Burns, K. H., & LaCava, J. (2019). LINE-1 ORF2p expression is nearly imperceptible in human cancers. Mobile dna, 11(1), [1]. https://doi.org/10.1186/s13100-019-0191-2
  5. Winczura, K., Domanski, M., & LaCava, J. (2019). Affinity Proteomic Analysis of the Human Exosome and Its Cofactor Complexes. (Methods in Molecular Biology). Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9822-7_15
  6. Ma, Y., Silveri, L., Lacava, J., Vassetzky, Y., & Dokudovskaya, S. (2019). Mtorc1 pathway in DNA damage response. Biopolymers and Cell, 35(3), 184. https://doi.org/10.7124/bc.0009BC
  7. 2018
  8. Pronier, E., Cifani, P., Merlinsky, T. R., Berman, K. B., Somasundara, A. V. H., Rampal, U. K., LaCava, J., Wei, K. E., Pastore, F., Maag, J. L., Park, J., Koche, R., Kentsis, A., & Levine, R. L. (2018). Targeting the CALR interactome in myeloproliferative neoplasms. JCI Insight, 3(22), [122703]. https://doi.org/10.1172/jci.insight.122703
  9. Zinoviev, A., Goyal, A., Jindal, S., LaCava, J., Komar, A. A., Rodnina, M. V., Hellen, C. U. T., & Pestova, T. V. (2018). Functions of unconventional mammalian translational GTPases GTPBP1 and GTPBP2. Genes & Development, 32(17-18), 1226-1241. https://doi.org/10.1101/gad.314724.118
  10. Chan, H. L., Beckedorff, F., Zhang, Y., Garcia-Huidobro, J., Jiang, H., Colaprico, A., Bilbao, D., Figueroa, M. E., LaCava, J., Shiekhattar, R., & Morey, L. (2018). Polycomb complexes associate with enhancers and promote oncogenic transcriptional programs in cancer through multiple mechanisms. Nature Communications, 9, [3377]. https://doi.org/10.1038/s41467-018-05728-x
  11. Altukhov, I., Taylor, M. S., Molloy, K. R., Mita, P., Jiang, H., Adney, E. M., Wudzinska, A., Badri, S., Ischenko, D. S., Eng, G., Burns, K. H., Fenyo, D., Chait, B. T., Alexeev, D., Rout, M. P., Boeke, J. D., & LaCava, J. (2018). Proteomic analysis of affinity captured LINE-1 macromolecular complexes. 25. https://doi.org/10.18699/BGRSSB-2018-003
  12. Taylor, M. S., Altukhov, I., Molloy, K. R., Mita, P., Jiang, H., Andey, E. M., Wudzinska, A., Badri, S., Ishenko, D., Eng, G., Burns, K. H., Fenyo, D., Chait, B. T., Alexeev, D., Rout, M. P., Boeke, J. D., & LaCava, J. (2018). Dissection of affinity captured LINE-1 macromolecular complexes. eLife, 7, [30094]. https://doi.org/10.7554/eLife.30094
  13. Mita, P., Wudzinska, A., Sun, X., Andrade, J., Nayak, S., Kahler, D. J., Badri, S., LaCava, J., Ueberheide, B., Yun, C. Y., Fenyo, D., & Boeke, J. D. (2018). LINE-1 protein localization and functional dynamics during the cell cycle. eLife, 7, [30058]. https://doi.org/10.7554/eLife.30058
  14. Winczura, K., Schmid, M., Iasillo, C., Molloy, K. R., Harder, L. M., Andersen, J. S., LaCava, J., & Jensen, T. H. (2018). Characterizing ZC3H18, a Multi-domain Protein at the Interface of RNA Production and Destruction Decisions. Cell reports, 22(1), 44-58. https://doi.org/10.1016/j.celrep.2017.12.037
  15. 2017
  16. Santanach, A., Blanco, E., Jiang, H., Molloy, K. R., Sanso, M., LaCava, J., Morey, L., & Di Croce, L. (2017). The Polycomb group protein CBX6 is an essential regulator of embryonic stem cell identity. Nature Communications, 8, [1235]. https://doi.org/10.1038/s41467-017-01464-w
  17. Molloy, K. R., Taylor, M. S., Altukhov, I., Mita, P., Jiang, H., Adney, E. M., Wudzinska, A., Ischenko, D., Burns, K. H., Fenyö, D., Chait, B. T., Alexeev, D., Rout, M. P., Boeke, J. D., & LaCava, J. (2017). Dissection of purified LINE-1 reveals distinct nuclear and cytoplasmic intermediates. bioRxiv. https://doi.org/10.1101/157818
  18. 2016
  19. Domanski, M., Upla, P., Rice, W. J., Molloy, K. R., Ketaren, N. E., Stokes, D. L., Jensen, T. H., Rout, M. P., & Lacava, J. (2016). Purification and analysis of endogenous human RNA exosome complexes. RNA, 22(9), 1467-1475. https://doi.org/10.1261/rna.057760.116
  20. LaCava, J., Fernandez-Martinez, J., & Rout, M. P. (2016). Native Elution of Yeast Protein Complexes Obtained by Affinity Capture. (7 ed.) (Cold Spring Harbor Protocols). https://doi.org/10.1101/pdb.prot087940
  21. LaCava, J., Fernandez-Martinez, J., Hakhverdyan, Z., & Rout, M. P. (2016). Optimized Affinity Capture of Yeast Protein Complexes. (7 ed.) (Cold Spring Harbor Protocols). https://doi.org/10.1101/pdb.prot087932
  22. LaCava, J., Fernandez-Martinez, J., Hakhverdyan, Z., & Rout, M. P. (2016). Protein complex purification by affinity capture. In Cold Spring Harbor Protocols: July (7 ed., Vol. 2016, pp. 601-605). (Cold Spring Harbor Protocols). https://doi.org/10.1101/pdb.top077545
  23. LaCava, J., Molloy, K. R., Fenyo, D., Taylor, M. S., Chait, B. T., Boeke, J. D., & Rout, M. P. (2016). Interactomic and Enzymatic Analyses of Distinct Affinity Isolated Human Retrotransposon Intermediates. FASEB Journal, 30(Supp. 1).
  24. Taylor, M. S., LaCava, J., Dai, L., Mita, P., Burns, K. H., Rout, M. P., & Boeke, J. D. (2016). Characterization of L1-Ribonucleoprotein Particles. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-3372-3_20
  25. 2015
  26. Hakhverdyan, Z., Domanski, M., Hough, L. E., Oroskar, A. A., Oroskar, A. R., Keegan, S., Dilworth, D. J., Molloy, K. R., Sherman, V., Aitchison, J. D., Fenyoe, D., Chait, B. T., Jensen, T. H., Rout, M. P., & LaCava, J. (2015). Rapid, optimized interactomic screening. Nature Methods, 12(6), 553-560. https://doi.org/10.1038/nmeth.3395
  27. 2014
  28. 2013
  29. Andersen, P. R., Domanski, M., Kristiansen, M. S., Storvall, H., Ntini, E., Verheggen, C., Schein, A., Bunkenborg, J., Poser, I., Hallais, M., Sandberg, R., Hyman, A., LaCava, J., Rout, M. P., Andersen, J. S., Bertrand, E., & Jensen, T. H. (2013). The human cap-binding complex is functionally connected to the nuclear RNA exosome. Nature Structural & Molecular Biology, 20(12), 1367-1376. https://doi.org/10.1038/nsmb.2703
  30. Taylor, M. S., LaCava, J., Mita, P., Molloy, K. R., Huang, C. R. L., Li, D., Adney, E. M., Jiang, H., Burns, K. H., Chait, B. T., Rout, M. P., Boeke, J. D., & Dai, L. (2013). Affinity Proteomics Reveals Human Host Factors Implicated in Discrete Stages of LINE-1 Retrotransposition. Cell, 155(5), 1034-1048. https://doi.org/10.1016/j.cell.2013.10.021
  31. LaCava, J., Hakhverdyan, Z., Domanski, M., Akgol, B., Saleem, R., Aitchison, J. D., Chait, B. T., Jensen, T. H., Fenyo, D., & Rout, M. P. (2013). Complementary tools supporting comprehensive mapping of protein complexes via affinity capture/mass spectrometry. Febs Journal, 280, 484. https://doi.org/10.1111/febs.12340
  32. 2012
  33. LaCava, J., Hakhverdyan, Z., Fenyo, D., Domanski, M., Oroskar, A., Oroskar, A., Hough, L., Jensen, T. H., Chait, B. T., & Rout, M. P. (2012). High-Throughput, Single-Step Purification of Affinity-Tagged Protein Complexes. FASEB Journal, 26.
  34. 2006
  35. 2005
  36. LaCava, J., Houseley, J., Saveanu, C., Petfalski, E., Thompson, E., Jacquier, A., & Tollervey, D. (2005). RNA degradation by the exosome is promoted by a nuclear polyadenylation complex. Cell, 121(5), 713-724. https://doi.org/10.1016/j.cell.2005.04.029
  37. 2004

ID: 84178686