Lecture Thorben Cordes
|18 November 2010||FWN-Building 5114.0004, Nijenborgh 4, 9747 AG, Groningen|
|Speaker:||Dr. Thorben Cordes|
|Affiliation:||University of Oxford, United Kingdom|
|Title:||High-resolution microscopy: single-molecule approaches to biology and optical super-resolution|
|Date:||Thu Nov 18, 2010|
|Host:||A. van Oijen|
|Telephone:||+31 50 363 9883|
In this talk an introduction on how to achieve sub-diffraction resolution in a standard fluorescence widefield microscope is given. It is shown how "super-resolution" images can be reconstructed by subsequent localization of single fluorophore labels using fluctuating emission or "blinking". The principles and photophysical requirements of localization-based super-resolution microscopy techniques (STORM/PALM type) are discussed and put into context of biological live-cell imaging. Finally, a novel characterization tool, based on the molecule-by-molecule assembly of DNA-oligomers, is introduced. The novel method is used to characterize parameters, such as the labelling density and photophysical properties of the fluorescent labels, which generally influence spatial and temporal resolution of localization-based super-resolution techniques.
The second part of the talk focuses on high-resolution spectroscopy using Förster resonance energy transfer (FRET) as a nanoscopic ruler for a mechanistic investigation of bacterial transcription by RNA polymerase.
|Last modified:||22 October 2012 2.30 p.m.|