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Lecture Thorben Cordes


18 november 2010 FWN-Building 5114.0004, Nijenborgh 4, 9747 AG, Groningen
Speaker: Dr. Thorben Cordes
Affiliation: University of Oxford, United Kingdom
Title: High-resolution microscopy: single-molecule approaches to biology and optical super-resolution
Date: Thu Nov 18, 2010
Start: 11.00
Location: FWN-Building 5114.0004
Host: A. van Oijen
Telephone: +31 50 363 9883


In this talk an introduction on how to achieve sub-diffraction resolution in a standard fluorescence widefield microscope is given. It is shown how "super-resolution" images can be reconstructed by subsequent localization of single fluorophore labels using fluctuating emission or "blinking". The principles and photophysical requirements of localization-based super-resolution microscopy techniques (STORM/PALM type) are discussed and put into context of biological live-cell imaging. Finally, a novel characterization tool, based on the molecule-by-molecule assembly of DNA-oligomers, is introduced. The novel method is used to characterize parameters, such as the labelling density and photophysical properties of the fluorescent labels, which generally influence spatial and temporal resolution of localization-based super-resolution techniques.

The second part of the talk focuses on high-resolution spectroscopy using Förster resonance energy transfer (FRET) as a nanoscopic ruler for a mechanistic investigation of bacterial transcription by RNA polymerase.

Laatst gewijzigd:22 oktober 2012 14:30