Lecture Peter Dedecker
|08 November 2010||FWN-Building 5116.0116, Nijenborgh 4, 9747 AG, Groningen|
|Speaker:||Dr. Peter Dedecker|
|Affiliation:||University of Leuven, Belgium|
|Title:||High-resolution microscopy using photoswitchable fluorophores|
|Date:||Mon Nov 8, 2010|
|Host:||Antoine van Oijen|
|Telephone:||+31 50 363 9883|
The past few years have seen the development of fluorescence microscopy with a resolution better than the diffraction limit. To a large extent this development has resulted from the observation of fluorophores at the single-molecule level, as well as the discovery of fluorophores that possess a dynamic fluorescence emission. These "smart" fluorophores allow the activation or deactivation of the fluorescence emission through irradiation with light. The resulting implications require the expansion of the classical view of fluorescence microscopy, and hence, provide a way to avoid the effects of the diffraction limit.
In this talk I will discuss my work on one of the first photoswitchable proteins to be discovered, the fluorescent protein Dronpa, as discuss its applications in subdiffraction microscopy. I will pay particular attention to the way in which the photophysics determine the imaging potential, and will highlight our current progress towards the engineering of 'smart labels'.
|Last modified:||22 October 2012 2.30 p.m.|