Lecture A. Tokmakoff
|20 January 2005||FWN-Building 5118.-152, Nijenborgh 4, 9747 AG, Groningen|
|Speaker:||Prof.dr. A. Tokmakoff|
|Affiliation:||Massachusetts Institute of Technology, Cambridge, MA, USA|
|Title:||Two-dimensional infrared spectroscopy of protein conformation and unfolding|
|Date:||Thu Jan 20, 2005|
|Telephone:||+31 50 363 4369|
Two-dimensional infrared spectroscopy is a tool for probing transient molecular structure in solution through a characterization of vibrational couplings. I will discuss the application of this method to the study of protein conformation through 2D IR spectroscopy of amide I vibrations. Vibrational couplings lead to delocalized states that are sensitive to secondary structure, but also influenced by disorder. 2D IR spectroscopy helps to characterize these variables and can thereby reveal information on mesoscopic structure in protein, and how it changes on thermal denaturing. Studies of transient thermal unfolding on nanosecond to millisecond time scales will also be presented.
This lecture is part of the MSC mini-symposium New developments in two-dimensional infrared spectroscopy. Please pre-register with Margriet Derix if you wish to attend one or more of its three lectures.
|Last modified:||22 October 2012 2.30 p.m.|