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Zernike Institute for Advanced Materials Colloquium Matthias Ballauff

Rooster

WanneerWaar
08 maart 2012 FWN-Building 5111.0080, Nijenborgh 4, 9747 AG, Groningen
Speaker: Prof. Dr. Matthias Ballauff
Affiliation:

Soft Matter and Functional Materials, Helmholtz-Zentrum Berlin für Materialien und Energie GmbH, Berlin, Germany

Title: Proteins and polyelectrolytes: a charged relationship
Date: Thu Mar 8, 2012
Start: 16.00 (Doors open and coffee available at 15.30)
Location: FWN-Building 5111.0080

Abstract

The interaction of proteins with solid surfaces is one of the central problems of modern biotechnology: On the one hand, adsorption on proteins must be prevented in many circumstances in order to prevent bacterial growth or biofouling, on the other hand, immobilization of enzymes on suitable solid supports is of considerable technical importance. Up to now, there is only a limited understanding of the driving forces for protein adsorption. Very often the so-called hydrophobic forces are held solely responsible for protein adsorption. However, Coulomb interactions are operative as well and may become decisive at low ionic strength. In my lecture I’ll present our recent work on this problem. The focus of our investigations is the basic understanding of the various forces leading to protein adsorption. The role of Coulombic forces will be discussed in detail. In addition to this a survey on recent experimental work done by us will be given. In particular, calorimetry has been used to demonstrate the central importance of entropic forces for protein adsorption [1]. Moreover, work done by small-angle x-ray scattering (SAXS) has revealed the dynamics of complexation and the spatial organization of proteins bound to polyelectrolytes [2]. All results obtained so far demonstrate that the interaction of proteins with surfaces can be understood in a semi-quantitative fashion by now.

1. K. Henzler, B. Haupt, K. Lauterbach, A. Wittemann, O. Borisov,M. Ballauff, Adsorption of beta-Lactoglobulin on Spherical Polyelectrolyte Brushes: Direct Proof of Counterion Release by Isothermal Titration Calorimetry, J Am Chem Soc, 2010, 132, 3159-3163; A. Becker, N. Welsch, M. Ballauff, in preparation

2. K. Henzler, S. Rosenfeldt, A. Wittemann, L. Harnau, S. Finet, T. Narayanan, M. Ballauff,

Directed motion of proteins along tethered polyelectrolytes , Phys. Rev. Lett . 2008, 100 , 158301; K. Henzler et al., Phys. Chem. Chem. Phys., submitted


Laatst gewijzigd:22 oktober 2012 14:30