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OnderzoekVan Swinderen Institute

Prof. dr. P. Tinnefeld, Technical University Braunschweig, Germany: Single-molecule and Superresolution Microscopy with DNA Origami

Roster

WhenWhere
18 October 2012 FWN-Building 5111.0080, Nijenborgh 4, 9747 AG, Groningen
Speaker: Prof. dr. Philip Tinnefeld
Affiliation: Technical University Braunschweig, Germany
Title: Single-molecule and Superresolution Microscopy with DNA Origami
Date: 18-10-2012
Start: 16.00 (Doors open and coffee available at 15.30)
Location: FWN-Building 5111.0080
Host: Thorben Cordes

Abstract

Scaffolded DNA origami is a revolutionary approach to design two- and three-dimensional objects of programmed shape and substantial dimensions. We used DNA origami as a biophysical tool to enhance single-molecule assays. Thereby, the DNA origami serves as molecular breadboard to arrange objects such as dyes and nanoparticles. We present examples of DNA origami applications including the development of nanoscopic rulers for 2D and 3D superresolution microscopy as well as for FRET, the measurement of ensemble and single-molecule kinetics without a change of the nano-environment, the switching of energy transfer pathways, and the enhancement of fluorescence signals.

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Last modified:12 September 2014 11.22 a.m.