Dr. I. Bald, Interdisciplinary Nanoscience Center, Aarhus University, Denmark and the Institute of Chemistry, University of Potsdam, Germany: Probing DNA Damage by DNA Nanotechnology
|31 May 2012||FWN-Building 5111.0080, Nijenborgh 4, 9747 AG, Groningen|
|Speaker:||Dr. Ilko Bald|
Interdisciplinary Nanoscience Center, Aarhus University, Denmark and the Institute of Chemistry, University of Potsdam, Germany
|Title:||Probing DNA Damage by DNA Nanotechnology|
|Start:||16.00 (Doors open and coffee available at 15.30)|
Very recent ground-breaking advances in DNA nanotechnology allow for the simple fabrication of complex two- and three-dimensional nanostructures. The DNA origami nanostructures are particularly versatile and can be easily functionalised with different chemical and biochemical moieties, for example higher-order DNA structures, proteins or nanoparticles. Consequently, numerous potential applications of DNA origami structures are currently discussed ranging from drug delivery over biosensing up to the construction of complex photonic biomolecular circuits.
We use the DNA origami technique to study how radiation damages complex DNA structures. In cancer radiation therapy predetermined doses of high-energy radi ation are administered to reduce tumours. On a molecular level a large extent of the cell damage is ascribed to the production of secondary low-energy electrons along the high-energy radiation track that induce DNA single and double strand breaks. The physico-chemical mechanisms of DNA radiation damage can currently only be described for idealized small model systems (such as individual nucleobases) and it is not known, which DNA nucleotide sequences and higher-order DNA structures are most susceptible to damage. We implemented the DNA origami technique to reveal the influence of complex DNA structures on the radiation damage characteristics, and preliminary results will be presented.
|Last modified:||12 September 2014 11.21 a.m.|