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OnderzoekVan Swinderen Institute

Physics: Prof. J. Hofkens: Studying (bio)molecular dynamics with single molecule spectroscopy


11 October 2007 FWN-Building 5111.0080, Nijenborgh 4, 9747 AG, Groningen
Speaker: Physics: Prof. Johan Hofkens
Affiliation: KU Leuven, Dept. of Chemistry
Title: Studying (bio)molecular dynamics with single molecule spectroscopy
Date: 11-10-2007
Start: 16.00
Location: FWN-Building 5111.0080


Over the last 15 years, single molecule spectroscopy (SMS) has been established as a new tool in the ever expanding range of spectroscopic methods. SMS is especially useful to study inhomogeneous systems. Biological systems are by their nature highly heterogeneous and as such perfect targets for SMS. From this it is clear that, next to biological samples, polymers form a study object of SMS as polymers are very often heterogeneous in their behavior. Many theories that describe polymer properties are based on a microscopic picture that now can be evaluated experimentally by applying single molecule techniques. Furthermore, single molecule techniques are very useful for the study of dynamic processes since no synchronization is needed when dynamic events are studied. In this contribution, we will show how different single molecule techniques can be used in the study of dynamic processes at the molecular level. The following topics will be discussed:

1) Defocused wide-field imaging of molecular rotation in [1-5].

2) Study of diffusion of individual polymers with wide-field microscopy [6]

3) Single molecule enzymatics [7,8]

4) Single molecule catalysis [9]


[1] D. Patra , I. Gregor and J. Enderlein, J. Phys. Chem. A 2004, 108, 6836-6841.

[2] M. Böhmer and J. Enderlein, J. Opt. Soc. Am. B 2003, 20, 554-559.

[3] W. Schroeyers, R. Vallée, D. Parta, J. Hofkens, S. Habuchi, T. Vosch, M. Cotlet, K. Müllen, J. Enderlein, F. C. De Schryver, J. Am. Chem. Soc. 2004, 126, 14310-14311.

[4] H.   Uji-i , S.M. Melnikov , A. Deres , G. Bergamini , F. De Schryver , A. Herrmann , K. Mullen , J. Enderlein , J. Hofkens Polymer 2006, 47, 2511-2518

[5] R. Vallee et al. J. Am. Chem. Soc. 2005, 127 (34), 12011-12020

[6] P.G. de Gennes, Scaling concepts in polymer physics, 1971

[7] K. Velonia, et al Angew. Chem., Int. Ed. 2005, 44 (4), 560-564

[8] O. flomenbom et al, Proc. Nat. Acad. Sci. USA 2005, 102 (7) 2368-2372

[9] M. Roeffaers et al, Nature 2006, 439, 572-575

Last modified:12 September 2014 11.21 a.m.