Zwitterionic hydrophilic interaction liquid chromatography-tandem mass spectrometry with HybridSPE-precipitation for the determination of intact cisplatin in human plasmaXie, F., Colin, P. & Van Bocxlaer, J., 1-Nov-2017, In : Talanta. 174, p. 171-178 8 p.
Research output: Contribution to journal › Article › Academic › peer-review
Cisplatin is a first-line chemotherapeutic for the treatment of a wide variety of cancers since its discovery in the 1960s. Although various techniques have been reported for the measurement of total platinum in biological matrices, such as inductively coupled plasma-mass spectrometry and derivatization procedures, a specific, sensitive and robust assay for the quantification of intact cisplatin is still lacking. Therefore, we present a rapid, selective, sensitive, and reliable UHPLC-MS/MS based method for the determination of intact cisplatin in human plasma in support of a Phase II clinical trial. The optimal chromatographic behavior of cisplatin was achieved on a Syncronis HILIC column (50 x 2.1 mm, 1.7 mu m, zwitterionic stationary phase). The retention behavior of cisplatin on this zwitterion-based stationary phase was well described by an adsorptive interaction model. A simple sample preparation based on protein precipitation combined with the removal of phospholipids by HybridSPE-precipitation was developed. The method was proven to be free of a relative matrix effect. The assay was validated within a range of 20 - 10,000 ng/mL using 100 mu L of plasma sample. The intra and inter day precisions were all less than 7.6%, and none of the bias was greater than 13.1%, thus corroborating that the developed method is precise and accurate. As a proof of concept, the assay has been successfully applied to plasma samples obtained from different patients who were enrolled in the Phase II trial and were treated with cisplatin.
|Number of pages||8|
|Publication status||Published - 1-Nov-2017|
- Cisplatin, UHPLC-MS/MS, HILIC, Matrix effects, HybirdSPE, Plasma, ICP-MS METHOD, POSTCOLUMN DERIVATIZATION, MONOHYDRATED COMPLEX, QUANTITATIVE-DETERMINATION, HYDRATED COMPLEXES, PLATINUM, PERFORMANCE, VALIDATION, BLOOD, PHOSPHOLIPIDS