Publication

YOUNG RED-BLOOD-CELLS - OBSERVATIONS ABOUT THE CYTOMETRIC AND MORPHOLOGIC ALTERATIONS IN THE BEGINNING OF THEIR LIFE

BOSCH, FH., WERRE, JM., ROERDINKHOLDERSTOELWINDER, B., HULS, TH., VANDERVEGT, SGL., WILLEKENS, FLA., BINS, M. & HALIE, MR., 1993, In : Clinical and laboratory haematology. 15, 4, p. 265-274 10 p.

Research output: Contribution to journalArticleAcademicpeer-review

  • FH BOSCH
  • JM WERRE
  • B ROERDINKHOLDERSTOELWINDER
  • TH HULS
  • SGL VANDERVEGT
  • FLA WILLEKENS
  • M BINS
  • MR HALIE

A recently described separation technique consisting of a combination of counterflow centrifugation and subsequent density (Percoll) separation was tested for its ability to enrich red cell populations with young cells in comparison to either separation technique alone. The relative age of every fraction was determined by HbAlc measurements, resulting in the lowest HbAlc for the combination method. Conventional reticulocyte counting and flowcytometric counting with thiazole orange indicated that in the youngest fractions the combination method showed the highest reticulocyte counts. There was a good correlation between manual and flowcytometric counting results. Radio-iron studies showed a two-fold enrichment with young cells in the fraction with the lowest HbAlc acquired by the combination technique in comparison to the other two methods. Cytometric measurements showed that the fractions with the lowest HbAlc were the ones with the highest MCV and MCH and the lowest MCHC. Besides loss of their RNA-material, young cells already seem to loose water and haemoglobin like older cells, resulting in a decrease of MCV and MCH and in increase in MCHC. It is concluded that combining counterflow centrifugation with subsequent density fractionation results in superior enrichment with young cells in comparison to the results of each method alone.

Original languageEnglish
Pages (from-to)265-274
Number of pages10
JournalClinical and laboratory haematology
Volume15
Issue number4
Publication statusPublished - 1993

    Keywords

  • RETICULOCYTES, HBALC, THIAZOLE ORANGE, FLOW-CYTOMETRY, THIAZOLE ORANGE, RETICULOCYTES, CENTRIFUGATION, ERYTHROCYTES, SEPARATION

ID: 6367157