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Yeast cells contain a heterogeneous population of peroxisomes that segregate asymmetrically during cell division

Kumar, S., de Boer, R. & van der Klei, I. J., 7-Feb-2018, In : Journal of Cell Science. 131, 3, 7 p., jcs.207522.

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DOI

Here we used fluorescence microscopy and a peroxisome-targeted tandem fluorescent protein timer to determine the relative age of peroxisomes in yeast. Our data indicate that yeast cells contain a heterogeneous population of relatively old and younger peroxisomes. During budding the peroxisome retention factor Inp1 selectively associates to the older organelles, which are retained in the mother cells. Inp2, a protein required for transport of peroxisomes to the bud, preferentially associates to younger organelles. Using a microfluidics device we demonstrate that the selective segregation of younger peroxisomes to the buds is carefully maintained during multiple budding events. The replicative lifespan of mother cells increased upon deletion of INP2, which resulted in the retention of all organelles in mother cells. These data suggest that in wild type yeast transport of aged and deteriorated peroxisomes to the bud is prevented, whereas the young and vital organelles are preferably transported to the newly forming buds.
Original languageEnglish
Article numberjcs.207522
Number of pages7
JournalJournal of Cell Science
Volume131
Issue number3
Early online date21-Dec-2017
Publication statusPublished - 7-Feb-2018

    Keywords

  • Peroxisome, Organelle inheritance, yeast, replicative lifespan, organelle, peroxisome inheritance, SACCHAROMYCES-CEREVISIAE; 3'-UNTRANSLATED REGION, HANSENULA-POLYMORPHA, FLUORESCENT PROTEIN, LIFE-SPAN, METABOLISM, FISSION, PROLIFERATION, CASSETTES, OXIDASE, DNM1P

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