Whole-Cell Patch-Clamp Recording of Mouse and Rat Inner Hair Cells in the Intact Organ of CortiGoutman, J. D. & Pyott, S., 4-Jun-2016, Auditory and Vestibular Research: Methods and Protocols. Sokolowski, B. (ed.). Springer, Vol. 1427. p. 471-485 15 p.
Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic › peer-review
Whole-cell patch clamping is a widely applied method to record currents across the entire membrane of a cell. This protocol describes application of this method to record currents from the sensory inner hair cells in the intact auditory sensory epithelium, the organ of Corti, isolated from rats or mice. This protocol particularly outlines the basic equipment required, provides instructions for the preparation of solutions and small equipment items, and methodology for recording voltage-activated and evoked synaptic currents from the inner hair cells.
|Title of host publication||Auditory and Vestibular Research|
|Subtitle of host publication||Methods and Protocols|
|Number of pages||15|
|Publication status||Published - 4-Jun-2016|