Whole-Cell Patch-Clamp Recording of Mouse and Rat Inner Hair Cells in the Intact Organ of Corti

Goutman, J. D. & Pyott, S., 4-Jun-2016, Auditory and Vestibular Research: Methods and Protocols. Sokolowski, B. (ed.). Springer, Vol. 1427. p. 471-485 15 p.

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Whole-cell patch clamping is a widely applied method to record currents across the entire membrane of a cell. This protocol describes application of this method to record currents from the sensory inner hair cells in the intact auditory sensory epithelium, the organ of Corti, isolated from rats or mice. This protocol particularly outlines the basic equipment required, provides instructions for the preparation of solutions and small equipment items, and methodology for recording voltage-activated and evoked synaptic currents from the inner hair cells.
Original languageEnglish
Title of host publicationAuditory and Vestibular Research
Subtitle of host publicationMethods and Protocols
EditorsBernd Sokolowski
Number of pages15
ISBN (Electronic)978-1-4939-3615-1
ISBN (Print)978-1-4939-3613-7
Publication statusPublished - 4-Jun-2016

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