Publication

Whole lifespan microscopic observation of budding yeast aging through a microfluidic dissection platform

Lee, S. S., Avalos Vizcarra, I., Huberts, D. H. E. W., Lee, L. P. & Heinemann, M., 27-Mar-2012, In : Proceedings of the National Academy of Sciences of the United States of America. 109, 13, p. 4916-4920 5 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Lee, S. S., Avalos Vizcarra, I., Huberts, D. H. E. W., Lee, L. P., & Heinemann, M. (2012). Whole lifespan microscopic observation of budding yeast aging through a microfluidic dissection platform. Proceedings of the National Academy of Sciences of the United States of America, 109(13), 4916-4920. https://doi.org/10.1073/pnas.1113505109

Author

Lee, Sung Sik ; Avalos Vizcarra, Ima ; Huberts, Daphne H E W ; Lee, Luke P ; Heinemann, Matthias. / Whole lifespan microscopic observation of budding yeast aging through a microfluidic dissection platform. In: Proceedings of the National Academy of Sciences of the United States of America. 2012 ; Vol. 109, No. 13. pp. 4916-4920.

Harvard

Lee, SS, Avalos Vizcarra, I, Huberts, DHEW, Lee, LP & Heinemann, M 2012, 'Whole lifespan microscopic observation of budding yeast aging through a microfluidic dissection platform', Proceedings of the National Academy of Sciences of the United States of America, vol. 109, no. 13, pp. 4916-4920. https://doi.org/10.1073/pnas.1113505109

Standard

Whole lifespan microscopic observation of budding yeast aging through a microfluidic dissection platform. / Lee, Sung Sik; Avalos Vizcarra, Ima; Huberts, Daphne H E W; Lee, Luke P; Heinemann, Matthias.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, No. 13, 27.03.2012, p. 4916-4920.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Lee SS, Avalos Vizcarra I, Huberts DHEW, Lee LP, Heinemann M. Whole lifespan microscopic observation of budding yeast aging through a microfluidic dissection platform. Proceedings of the National Academy of Sciences of the United States of America. 2012 Mar 27;109(13):4916-4920. https://doi.org/10.1073/pnas.1113505109


BibTeX

@article{5fe8f174547e442d849b9994d8bf99d2,
title = "Whole lifespan microscopic observation of budding yeast aging through a microfluidic dissection platform",
abstract = "Important insights into aging have been generated with the genetically tractable and short-lived budding yeast. However, it is still impossible today to continuously track cells by high-resolution microscopic imaging (e.g., fluorescent imaging) throughout their entire lifespan. Instead, the field still needs to rely on a 50-y-old laborious and time-consuming method to assess the lifespan of yeast cells and to isolate differentially aged cells for microscopic snapshots via manual dissection of daughter cells from the larger mother cell. Here, we are unique in achieving continuous and high-resolution microscopic imaging of the entire replicative lifespan of single yeast cells. Our microfluidic dissection platform features an optically prealigned single focal plane and an integrated array of soft elastomer-based micropads, used together to allow for trapping of mother cells, removal of daughter cells, monitoring gradual changes in aging, and unprecedented microscopic imaging of the whole aging process. Using the platform, we found remarkable age-associated changes in phenotypes (e.g., that cells can show strikingly differential cell and vacuole morphologies at the moment of their deaths), indicating substantial heterogeneity in cell aging and death. We envision the microfluidic dissection platform to become a major tool in aging research.",
keywords = "Microfluidics, Microscopy, Fluorescence, Phenotype, Saccharomycetales, Time Factors",
author = "Lee, {Sung Sik} and {Avalos Vizcarra}, Ima and Huberts, {Daphne H E W} and Lee, {Luke P} and Matthias Heinemann",
year = "2012",
month = mar,
day = "27",
doi = "10.1073/pnas.1113505109",
language = "English",
volume = "109",
pages = "4916--4920",
journal = "Proceedings of the National Academy of Science of the United States of America",
issn = "0027-8424",
publisher = "NATL ACAD SCIENCES",
number = "13",

}

RIS

TY - JOUR

T1 - Whole lifespan microscopic observation of budding yeast aging through a microfluidic dissection platform

AU - Lee, Sung Sik

AU - Avalos Vizcarra, Ima

AU - Huberts, Daphne H E W

AU - Lee, Luke P

AU - Heinemann, Matthias

PY - 2012/3/27

Y1 - 2012/3/27

N2 - Important insights into aging have been generated with the genetically tractable and short-lived budding yeast. However, it is still impossible today to continuously track cells by high-resolution microscopic imaging (e.g., fluorescent imaging) throughout their entire lifespan. Instead, the field still needs to rely on a 50-y-old laborious and time-consuming method to assess the lifespan of yeast cells and to isolate differentially aged cells for microscopic snapshots via manual dissection of daughter cells from the larger mother cell. Here, we are unique in achieving continuous and high-resolution microscopic imaging of the entire replicative lifespan of single yeast cells. Our microfluidic dissection platform features an optically prealigned single focal plane and an integrated array of soft elastomer-based micropads, used together to allow for trapping of mother cells, removal of daughter cells, monitoring gradual changes in aging, and unprecedented microscopic imaging of the whole aging process. Using the platform, we found remarkable age-associated changes in phenotypes (e.g., that cells can show strikingly differential cell and vacuole morphologies at the moment of their deaths), indicating substantial heterogeneity in cell aging and death. We envision the microfluidic dissection platform to become a major tool in aging research.

AB - Important insights into aging have been generated with the genetically tractable and short-lived budding yeast. However, it is still impossible today to continuously track cells by high-resolution microscopic imaging (e.g., fluorescent imaging) throughout their entire lifespan. Instead, the field still needs to rely on a 50-y-old laborious and time-consuming method to assess the lifespan of yeast cells and to isolate differentially aged cells for microscopic snapshots via manual dissection of daughter cells from the larger mother cell. Here, we are unique in achieving continuous and high-resolution microscopic imaging of the entire replicative lifespan of single yeast cells. Our microfluidic dissection platform features an optically prealigned single focal plane and an integrated array of soft elastomer-based micropads, used together to allow for trapping of mother cells, removal of daughter cells, monitoring gradual changes in aging, and unprecedented microscopic imaging of the whole aging process. Using the platform, we found remarkable age-associated changes in phenotypes (e.g., that cells can show strikingly differential cell and vacuole morphologies at the moment of their deaths), indicating substantial heterogeneity in cell aging and death. We envision the microfluidic dissection platform to become a major tool in aging research.

KW - Microfluidics

KW - Microscopy, Fluorescence

KW - Phenotype

KW - Saccharomycetales

KW - Time Factors

U2 - 10.1073/pnas.1113505109

DO - 10.1073/pnas.1113505109

M3 - Article

C2 - 22421136

VL - 109

SP - 4916

EP - 4920

JO - Proceedings of the National Academy of Science of the United States of America

JF - Proceedings of the National Academy of Science of the United States of America

SN - 0027-8424

IS - 13

ER -

ID: 5533071