Vitamin B-6 vitamers in human plasma and cerebrospinal fluid

Albersen, M., Bosma, M., Luykx, J. J., Jans, J. J. M., Bakker, S. C., Strengman, E., Borgdorff, P. J., Keijzers, P. J. M., van Dongen, E. P. A., Bruins, P., de Sain-van der Velden, M. G. M., Visser, G., Knoers, N. V. V. A. M., Ophoff, R. A. & Verhoeven-Duif, N. M., Aug-2014, In : American Journal of Clinical Nutrition. 100, 2, p. 587-592 6 p.

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  • Vitamin B-6 vitamers in human plasma and cerebrospinal fluid

    Final publisher's version, 213 KB, PDF-document


  • Monique Albersen
  • Marjolein Bosma
  • Jurjen J Luykx
  • Judith J M Jans
  • Steven C Bakker
  • Eric Strengman
  • Paul J Borgdorff
  • Peter J M Keijzers
  • Eric P A van Dongen
  • Peter Bruins
  • Monique G M de Sain-van der Velden
  • Gepke Visser
  • Nine V V A M Knoers
  • Roel A Ophoff
  • Nanda M Verhoeven-Duif

BACKGROUND: Vitamin B-6 comprises a group of 6 interrelated vitamers and is essential for numerous physiologic processes, including brain functioning. Genetic disorders disrupting vitamin B-6 metabolism have severe clinical consequences.

OBJECTIVE: To adequately diagnose known and novel disorders in vitamin B-6 metabolism, a reference set is required containing information on all vitamin B-6 vitamers in plasma and cerebrospinal fluid (CSF).

DESIGN: Concentrations of vitamin B-6 vitamers in the plasma and CSF of 533 adult subjects were measured by ultra high-performance liquid chromatography-tandem mass spectrometry.

RESULTS: The relative vitamin B-6 vitamer composition of plasma [pyridoxal phosphate (PLP) > pyridoxic acid (PA) > pyridoxal] differed from that of CSF (pyridoxal > PLP > PA > pyridoxamine). Sex influenced vitamin B-6 vitamer concentrations in plasma and CSF and should therefore be taken into account when interpreting vitamin B-6 vitamer concentrations. The strict ratios and strong correlations between vitamin B-6 vitamers point to a tight regulation of vitamin B-6 vitamer concentrations in blood and CSF. Given the unique design of this study, with simultaneously withdrawn blood and CSF from a large number of subjects, reliable CSF:plasma ratios and correlations of vitamin B-6 vitamers could be established.

CONCLUSIONS: We provide an extensive reference set of vitamin B-6 vitamer concentrations in plasma and CSF. In addition to providing insight on the regulation of individual vitamers and their intercompartmental distribution, we anticipate that these data will prove to be a valuable reference set for the diagnosis and treatment of conditions associated with altered vitamin B-6 metabolism.

Original languageEnglish
Pages (from-to)587-592
Number of pages6
JournalAmerican Journal of Clinical Nutrition
Issue number2
Publication statusPublished - Aug-2014


  • Adolescent, Adult, Algorithms, Chromatography, High Pressure Liquid, Female, Humans, Limit of Detection, Male, Middle Aged, Netherlands, Pyridoxal/blood, Pyridoxal Phosphate/blood, Pyridoxamine/cerebrospinal fluid, Pyridoxic Acid/blood, Reference Values, Reproducibility of Results, Sex Characteristics, Tandem Mass Spectrometry, Vitamin B 6/blood, Young Adult

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