Visualization of Single Molecules Building a Viral Capsid Protein Lattice through Stochastic Pathways

Valbuena, A., Maity, S., Mateu, M. G. & Roos, W. H., 28-Jul-2020, In : Acs Nano. 14, 7, p. 8724-8734 11 p.

Research output: Contribution to journalArticleAcademicpeer-review

Direct visualization of pathways followed by single molecules while they spontaneously self-assemble into supramolecular biological machines may provide fundamental knowledge to guide molecular therapeutics and the bottom-up design of nanomaterials and nanodevices. Here, high-speed atomic force microscopy is used to visualize self-assembly of the bidimensional lattice of protein molecules that constitutes the framework of the mature human immunodeficiency virus capsid. By real-time imaging of the assembly reaction, individual transient intermediates and reaction pathways followed by single molecules could be revealed. As when assembling a jigsaw puzzle, the capsid protein lattice is randomly built. Lattice patches grow independently from separate nucleation events whereby individual molecules follow different paths. Protein subunits can be added individually, while others form oligomers before joining a lattice or are occasionally removed from the latter. Direct real-time imaging of supramolecular self-assembly has revealed a complex, chaotic process involving multiple routes followed by individual molecules that are inaccessible to bulk (averaging) techniques.

Original languageEnglish
Pages (from-to)8724-8734
Number of pages11
JournalAcs Nano
Issue number7
Early online date7-Jul-2020
Publication statusPublished - 28-Jul-2020


  • self-assembly, nanomaterials, virus, capsid protein, single-molecule, high-speed atomic force microscopy, SELF-ASSEMBLY PATHWAY, VIRUS, MICROSCOPY, DYNAMICS, POLYMERIZATION, EQUILIBRIUM, MECHANISMS, NUCLEATION, STABILITY, KINETICS

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